MD16G1104200.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000354
Physical Location & Seq
Forward (+)
7305685 .. 7306291
607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1104200.v1.1.491

Sequence Viewer

Length: 366 bp
ATGCTCCTCCACCTCGGCCGCATCCCCACGATCATAATCTCTTCCGCCGAAGCAGCCAAAGATGTCTTAAAAACCAATGATCTCTACTGCTGCAGCAGACCCTCCTCCACCGGTGCTCGCAGGCTCACCTACAACTACCTAGACATTGGATTTTCGCCTTACGGTGATTACTGGAGAGAGATGAGAAAAATATGCGTGCGCGAGCTTTTCAGTGTGAAGAGAGTCCAGTCATACGGGACAATCCGGGAAGAAGAAGTGGATAAAATGGTCAATTCAATCTCTGCCCCTTCATCTTCTGGTGTTCCTGTTGATCTCACGGAAAAGTTGTTTGCTTTCGCAGCTATTATAATTTTTAGGATTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

122

Amino Acids

13.64

Weight (kDa)

8.84

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 120 3.5e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AccII CGCG 1 cut(s) 201
AciI CCGC 2 cut(s) 19, 45
AcoI YGGCCR 1 cut(s) 16
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 276
AluBI AGCT 2 cut(s) 205, 341
AluI AGCT 2 cut(s) 205, 341
Alw21I GWGCWC 1 cut(s) 118
AoxI GGCC 1 cut(s) 16
ApeKI GCWGC 4 cut(s) 53, 90, 93, 338
AsiGI ACCGGT 1 cut(s) 110
AspLEI GCGC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 118, 176
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 4 cut(s) 65, 77, 105, 350
BcnI CCSGG 1 cut(s) 245
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 5 cut(s) 19, 54, 91, 94, 339
BlsI GCNGC 5 cut(s) 20, 55, 92, 95, 340
Bme1390I CCNGG 1 cut(s) 245
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 176, 226
Bse8I GATNNNNATC 1 cut(s) 35
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 1 cut(s) 21
BseJI GATNNNNATC 1 cut(s) 35
BseNI ACTGG 2 cut(s) 176, 226
BseRI GAGGAG 1 cut(s) 94
BseX3I CGGCCG 1 cut(s) 16
BseXI GCAGC 4 cut(s) 65, 77, 105, 350
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 18
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 111, 244
BslFI GGGAC 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 1 cut(s) 18
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 3 cut(s) 30, 79, 310
BspACI CCGC 2 cut(s) 19, 45
BspANI GGCC 1 cut(s) 18
BspFNI CGCG 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 2 cut(s) 176, 226
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 3 cut(s) 30, 79, 310
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 2 cut(s) 46, 212
BstC8I GCNNGC 4 cut(s) 118, 122, 197, 203
BstF5I GGATG 1 cut(s) 21
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 3 cut(s) 33, 82, 313
BstMBI GATC 3 cut(s) 30, 79, 310
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 2 cut(s) 53, 338
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 4 cut(s) 65, 77, 105, 350
BstZI CGGCCG 1 cut(s) 16
BsuRI GGCC 1 cut(s) 18
BtsCI GGATG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 4 cut(s) 118, 122, 197, 203
CfoI GCGC 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 110
CspAI ACCGGT 1 cut(s) 110
CviJI RGCY 5 cut(s) 18, 56, 124, 205, 341
CviKI_1 RGCY 5 cut(s) 18, 56, 124, 205, 341
DpnI GATC 3 cut(s) 32, 81, 312
DpnII GATC 3 cut(s) 30, 79, 310
EaeI YGGCCR 1 cut(s) 16
EagI CGGCCG 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 46, 212
EarI CTCTTC 2 cut(s) 46, 212
EciI GGCGGA 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 16
Eco52I CGGCCG 1 cut(s) 16
FaiI YATR 4 cut(s) 35, 193, 232, 347
FaqI GGGAC 1 cut(s) 250
Fnu4HI GCNGC 5 cut(s) 19, 54, 91, 94, 339
FokI GGATG 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 19, 54, 91, 94, 339
FspBI CTAG 1 cut(s) 140
GlaI GCGC 1 cut(s) 200
GluI GCNGC 5 cut(s) 19, 54, 91, 94, 339
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 1 cut(s) 18
HapII CCGG 2 cut(s) 111, 244
HhaI GCGC 1 cut(s) 201
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HinfI GANTC 1 cut(s) 222
HpaII CCGG 2 cut(s) 111, 244
HphI GGTGA 2 cut(s) 118, 176
HpyAV CCTTC 1 cut(s) 297
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 338
HspAI GCGC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 30, 79, 310
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 7 cut(s) 106, 124, 157, 239, 257, 282, 318
Lsp1109I GCAGC 4 cut(s) 65, 77, 105, 350
LweI GCATC 1 cut(s) 30
MaeI CTAG 1 cut(s) 140
MalI GATC 3 cut(s) 32, 81, 312
MboI GATC 3 cut(s) 30, 79, 310
MboII GAAGA 5 cut(s) 33, 229, 260, 263, 285
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 2 cut(s) 271, 348
MlyI GAGTC 1 cut(s) 231
MnlI CCTC 4 cut(s) 17, 23, 112, 115
MseI TTAA 1 cut(s) 68
MspI CCGG 2 cut(s) 111, 244
MspR9I CCNGG 1 cut(s) 245
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 53, 338
NciI CCSGG 1 cut(s) 245
NdeII GATC 3 cut(s) 30, 79, 310
PfoI TCCNGGA 1 cut(s) 243
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 5 cut(s) 20, 55, 92, 95, 340
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PsiI TTATAA 1 cut(s) 347
PstI CTGCAG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 5 cut(s) 19, 54, 91, 94, 339
Sau3AI GATC 3 cut(s) 30, 79, 310
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 118
SetI ASST 5 cut(s) 15, 131, 141, 207, 343
SfaNI GCATC 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 91
SgrAI CRCCGGYG 1 cut(s) 110
Sse9I AATT 2 cut(s) 271, 348
SsiI CCGC 2 cut(s) 19, 45
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 164
TasI AATT 2 cut(s) 271, 348
TauI GCSGC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 1 cut(s) 217
TseI GCWGC 4 cut(s) 53, 90, 93, 338
TspDTI ATGAA 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 1 cut(s) 217
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.