Rh4CG233100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000354
Physical Location & Seq
Reverse (-)
47332411 .. 47333373
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG233100.1

Sequence Viewer

Length: 963 bp
ATGGCTGACAACACCGTCCTACTAATCTTATGGATTCCATTTCTCCTCCTTCTCCCTTTACTACTGCTTTTACTGAGAAAGCAGATCAATAAGCCACAAAAGCACTTCCCACCAAGCCCTCCTAAGCTTCCCATTATAGGCAACCTCCACCAGTTTACTGAATCTGGTTCATCACTTCATCAAAACCTATGGAGACTATCACAGAAATACGGACCACTCATGCTCTTACACATGGGTCGCAGACCGACCCTCATAATCTCTTCAGCTGAAGCAGCAAAACAGACCTTGAAAGATAATGATCTCCACTGTTGCAGTAGACCGGCCTCCTTGGGATCTCGCAGACTCACATTTAACTATCTAGACATAGCATTTTCGCCTTACGGTGAATATTGGCGAGAGATAAGAAAGATATGTGTGCATGAGATTTTCAGTGCGAAAGGGGTTGAGTCGTATAGGTCTATTAGAGAAGAAGAAGTCATGAAGATGATTGATTCAATCTCTGACGCATCAGCTTCATCTTCTCCTGTTAACCTTACTGAGAAGTTGTTTGCTCTCATGAGCAGTATAATTTTCAGGATTGTTTTCGGGACAAGTTTCCAAGGGAGCGATTTGGAGCATGAAAGATTTCTTCGAGTGCTTCTTGATAGTCGAGATATGCTTACAGGCTTCTCATCATGCGCAGCTGATTTCTTTCCTCCATGTATAGGGTGGATCATAGACAGGATCTCCGGTAAACATAGAGAGTTTGACAGAGTTTCCAGGGAAGTTCATCACTTTTTTCAGCAAGCGATTGATGATCATCTCAAACCTGGGAGGACACAACAGGAGCATGAAGATATAGTTGATGTGCTGCTTAAAATGGTCAAGAAGCAAACTGAGATTGGAGCTGCTCATCTCGGTCATAATAACATCAAGGCAGTTCTCTTGGTAAGCTGCGAACTAATTCAAATCACGGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

320

Amino Acids

36.45

Weight (kDa)

8.49

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 37 - 308 1.4e-35 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
Acc16I TGCGCA 1 cut(s) 679
AccI GTMKAC 1 cut(s) 316
AclWI GGATC 3 cut(s) 340, 719, 731
AcuI CTGAAG 2 cut(s) 246, 288
AfiI CCNNNNNNNGG 2 cut(s) 137, 704
AgsI TTSAA 3 cut(s) 289, 495, 947
AjnI CCWGG 2 cut(s) 758, 808
AluBI AGCT 6 cut(s) 127, 266, 512, 683, 887, 933
AluI AGCT 6 cut(s) 127, 266, 512, 683, 887, 933
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 3 cut(s) 340, 719, 731
AoxI GGCC 2 cut(s) 321, 954
ApeKI GCWGC 5 cut(s) 272, 680, 850, 887, 933
Asp700I GAANNNNTTC 2 cut(s) 624, 942
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 212
BbvI GCAGC 5 cut(s) 284, 692, 837, 874, 920
BciT130I CCWGG 2 cut(s) 760, 810
BclI TGATCA 1 cut(s) 796
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 1 cut(s) 359
BisI GCNGC 5 cut(s) 273, 681, 851, 888, 934
BlsI GCNGC 5 cut(s) 274, 682, 852, 889, 935
Bme1390I CCNGG 2 cut(s) 760, 810
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmrFI CCNGG 2 cut(s) 760, 810
BmsI GCATC 1 cut(s) 515
Bpu10I CCTNAGC 1 cut(s) 123
BsaBI GATNNNNATC 2 cut(s) 297, 798
BsaJI CCNNGG 4 cut(s) 327, 598, 759, 809
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 704
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 151
Bse8I GATNNNNATC 2 cut(s) 297, 798
BseBI CCWGG 2 cut(s) 760, 810
BseDI CCNNGG 4 cut(s) 327, 598, 759, 809
BseJI GATNNNNATC 2 cut(s) 297, 798
BseLI CCNNNNNNNGG 2 cut(s) 137, 704
BseMII CTCAG 3 cut(s) 65, 528, 867
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 5 cut(s) 284, 692, 837, 874, 920
BshFI GGCC 2 cut(s) 323, 956
BsiSI CCGG 2 cut(s) 320, 729
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 2 cut(s) 137, 704
BsmAI GTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 2 cut(s) 323, 956
Bsp143I GATC 6 cut(s) 84, 298, 332, 711, 723, 796
BspANI GGCC 2 cut(s) 323, 956
BspCNI CTCAG 3 cut(s) 66, 529, 868
BspHI TCATGA 2 cut(s) 477, 555
BspPI GGATC 3 cut(s) 340, 719, 731
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 4 cut(s) 327, 598, 759, 809
BssMI GATC 6 cut(s) 84, 298, 332, 711, 723, 796
BssT1I CCWWGG 2 cut(s) 327, 598
Bst2UI CCWGG 2 cut(s) 760, 810
Bst4CI ACNGT 3 cut(s) 16, 308, 383
Bst6I CTCTTC 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 4 cut(s) 74, 123, 537, 876
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 6 cut(s) 87, 301, 335, 714, 726, 799
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 6 cut(s) 84, 298, 332, 711, 723, 796
BstMWI GCNNNNNNNGC 2 cut(s) 100, 272
BstNI CCWGG 2 cut(s) 760, 810
BstSCI CCNGG 2 cut(s) 758, 808
BstV1I GCAGC 5 cut(s) 284, 692, 837, 874, 920
BstX2I RGATCY 2 cut(s) 332, 723
BstYI RGATCY 2 cut(s) 332, 723
BsuRI GGCC 2 cut(s) 323, 956
BtsIMutI CAGTG 2 cut(s) 304, 436
Cac8I GCNNGC 1 cut(s) 786
CciI TCATGA 2 cut(s) 477, 555
CfoI GCGC 1 cut(s) 680
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 1 cut(s) 512
CviAII CATG 9 cut(s) 220, 232, 419, 478, 556, 617, 675, 699, 830
DdeI CTNAG 4 cut(s) 74, 123, 537, 876
DpnI GATC 6 cut(s) 86, 300, 334, 713, 725, 798
DpnII GATC 6 cut(s) 84, 298, 332, 711, 723, 796
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 327, 598
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 246, 288
EcoRII CCWGG 2 cut(s) 758, 808
EcoT14I CCWWGG 2 cut(s) 327, 598
ErhI CCWWGG 2 cut(s) 327, 598
FaeI CATG 9 cut(s) 223, 235, 422, 481, 559, 620, 678, 702, 833
FaqI GGGAC 1 cut(s) 601
FatI CATG 9 cut(s) 219, 231, 418, 477, 555, 616, 674, 698, 829
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 1 cut(s) 316
Fnu4HI GCNGC 5 cut(s) 273, 681, 851, 888, 934
Fsp4HI GCNGC 5 cut(s) 273, 681, 851, 888, 934
FspBI CTAG 1 cut(s) 359
FspI TGCGCA 1 cut(s) 679
GlaI GCGC 1 cut(s) 679
GluI GCNGC 5 cut(s) 273, 681, 851, 888, 934
HaeIII GGCC 2 cut(s) 323, 956
HapII CCGG 2 cut(s) 320, 729
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 680
Hin1II CATG 9 cut(s) 223, 235, 422, 481, 559, 620, 678, 702, 833
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 5 cut(s) 34, 161, 342, 446, 491
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 2 cut(s) 320, 729
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 4 cut(s) 156, 317, 529, 734
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 8 cut(s) 359, 478, 556, 574, 586, 641, 650, 865
Hpy8I GTNNAC 4 cut(s) 156, 317, 529, 734
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 16, 308, 383
HpyCH4V TGCA 2 cut(s) 312, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 272
HpyF3I CTNAG 4 cut(s) 74, 123, 537, 876
Hsp92II CATG 9 cut(s) 223, 235, 422, 481, 559, 620, 678, 702, 833
HspAI GCGC 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 796
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 6 cut(s) 84, 298, 332, 711, 723, 796
LmnI GCTCC 4 cut(s) 603, 613, 826, 884
Lsp1109I GCAGC 5 cut(s) 284, 692, 837, 874, 920
LweI GCATC 1 cut(s) 515
MaeI CTAG 1 cut(s) 359
MalI GATC 6 cut(s) 86, 300, 334, 713, 725, 798
MboI GATC 6 cut(s) 84, 298, 332, 711, 723, 796
MboII GAAGA 7 cut(s) 252, 479, 482, 493, 510, 620, 845
MflI RGATCY 2 cut(s) 332, 723
MluCI AATT 2 cut(s) 567, 942
MlyI GAGTC 2 cut(s) 336, 455
MnlI CCTC 7 cut(s) 56, 129, 155, 260, 334, 705, 807
MroXI GAANNNNTTC 2 cut(s) 624, 942
MseI TTAA 4 cut(s) 351, 528, 855, 961
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 266, 683
MspI CCGG 2 cut(s) 320, 729
MspR9I CCNGG 2 cut(s) 760, 810
MvaI CCWGG 2 cut(s) 760, 810
MwoI GCNNNNNNNGC 2 cut(s) 100, 272
NdeII GATC 6 cut(s) 84, 298, 332, 711, 723, 796
NlaIII CATG 9 cut(s) 223, 235, 422, 481, 559, 620, 678, 702, 833
NsbI TGCGCA 1 cut(s) 679
PagI TCATGA 2 cut(s) 477, 555
PdmI GAANNNNTTC 2 cut(s) 624, 942
PfeI GAWTC 3 cut(s) 34, 161, 491
PkrI GCNGC 5 cut(s) 274, 682, 852, 889, 935
PleI GAGTC 2 cut(s) 336, 454
PpsI GAGTC 2 cut(s) 336, 454
Psp6I CCWGG 2 cut(s) 758, 808
PspGI CCWGG 2 cut(s) 758, 808
PspPI GGNCC 1 cut(s) 212
PsuI RGATCY 2 cut(s) 332, 723
PvuII CAGCTG 2 cut(s) 266, 683
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 4 cut(s) 351, 528, 855, 961
SatI GCNGC 5 cut(s) 273, 681, 851, 888, 934
Sau3AI GATC 6 cut(s) 84, 298, 332, 711, 723, 796
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 2 cut(s) 336, 455
ScrFI CCNGG 2 cut(s) 760, 810
SfaNI GCATC 1 cut(s) 515
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 2 cut(s) 567, 942
SspI AATATT 1 cut(s) 389
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 758, 808
StyI CCWWGG 2 cut(s) 327, 598
TaaI ACNGT 3 cut(s) 16, 308, 383
TaqI TCGA 2 cut(s) 631, 649
TaqII GACCGA 2 cut(s) 259, 887
TasI AATT 2 cut(s) 567, 942
TfiI GAWTC 3 cut(s) 34, 161, 491
Tru1I TTAA 4 cut(s) 351, 528, 855, 961
Tru9I TTAA 4 cut(s) 351, 528, 855, 961
TscAI CASTG 2 cut(s) 311, 436
TseI GCWGC 5 cut(s) 272, 680, 850, 887, 933
TspDTI ATGAA 7 cut(s) 159, 167, 494, 504, 633, 758, 846
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 2 cut(s) 311, 436
VpaK11BI GGWCC 1 cut(s) 212
XbaI TCTAGA 1 cut(s) 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XmiI GTMKAC 1 cut(s) 316
XmnI GAANNNNTTC 2 cut(s) 624, 942
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.