MD16G1104300.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000354
Physical Location & Seq
Forward (+)
7306293 .. 7307796
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1104300.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCTCGGAGGCATGTACGGAGCTGACTACTTTCCGTCTTGGATCTGGTGGGTTATCGACAGGGTTTCCTGTATGCACAAGGAGTTGGATAGGATTTCGAAGGAGTTGGATGGTTTTTTCCAGCAGGTGATTGACGATCATCTGAAACCTAGGAGGAGAGTTGACGAACAAACTCATGAAGATATTGTTGACGTCCTGCTGAACATAGTGAAGGAGCGTAGTGAGTTAAGAGCTTCTAATCATCTTGGTCACAACAATATCAAGGCAGTCCTCTTGAATCTATTTTTAGGTGGAATAGACACAGGCGCAATTACAATGGTGTGGGCGATGGCAGAGCTGGCTAACAACCCTGGACTGATGAAGAAAGCACAAGAAGAAGTCAGAAAATACATCGGAAACAAAGGAAAAGTCACAGAAACTGAAACAGATCAGCTCCAATACCTCAAGATGGTAATCAAAGAAACCCTAGGACTGCATCCTCTAGCTCCAATGATCCTTCCCAGGGAAACCACGTCCCGTTGCAAAATCCAGGGTTATGACATTGACCCCAAAACCCTAGTTCTTGTTAATGCATGGGCAATCGCACGCAACCCCGGGTTTTGGAAGGACCCAGAAGAGTTCATCCCTGAAAGGTTTGATGGAAGCTCGGTTGATTACCAAGGACAACATTTTGAGTTTCTGCCGTTCGGAGCTGGTCGAAGAGTTTGTCCAGGAATATATATGGGAACGACAACGGTGAAGCTTGGACTTGCAAATCTTGTCTACTGGTTCGACTGGAAATTACCTGATGGAATTGTGGAAGATCTCGACATGGAAGAAACAAGCGGCTCCCCTTCCCTCACTGTCTCTAAGAAAACTCCTCTCAACCTCTCAAATTCTCTCAGAAAGAGTAAAGAGGTTCTTCTCTATGAGCTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

307

Amino Acids

34.84

Weight (kDa)

5.72

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 10 - 273 8.3e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 762
AciI CCGC 1 cut(s) 825
AclWI GGATC 2 cut(s) 50, 487
AcsI RAATTY 1 cut(s) 874
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 3 cut(s) 448, 502, 600
AgsI TTSAA 1 cut(s) 277
AjiI CACGTC 1 cut(s) 513
AjnI CCWGG 4 cut(s) 349, 500, 528, 709
AluBI AGCT 9 cut(s) 23, 233, 337, 433, 485, 645, 692, 742, 913
AluI AGCT 9 cut(s) 23, 233, 337, 433, 485, 645, 692, 742, 913
Alw26I GTCTC 1 cut(s) 850
AlwI GGATC 2 cut(s) 50, 487
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 593
ApoI RAATTY 1 cut(s) 874
ArsI GACNNNNNNTTYG 4 cut(s) 393, 425, 691, 723
AspA2I CCTAGG 2 cut(s) 149, 466
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 607
AsuC2I CCSGG 2 cut(s) 594, 595
AsuHPI GGTGA 2 cut(s) 139, 748
AsuII TTCGAA 1 cut(s) 98
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 607
AvrII CCTAGG 2 cut(s) 149, 466
BccI CCATC 5 cut(s) 104, 322, 442, 632, 782
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 4 cut(s) 351, 502, 530, 711
BcnI CCSGG 2 cut(s) 594, 595
BcoDI GTCTC 1 cut(s) 850
BfaI CTAG 5 cut(s) 150, 467, 482, 557, 919
BfuAI ACCTGC 1 cut(s) 115
BglII AGATCT 1 cut(s) 802
BisI GCNGC 1 cut(s) 826
BlnI CCTAGG 2 cut(s) 149, 466
BlsI GCNGC 1 cut(s) 827
Bme1390I CCNGG 6 cut(s) 351, 502, 530, 594, 595, 711
Bme18I GGWCC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 2 cut(s) 609, 829
BmrFI CCNGG 6 cut(s) 351, 502, 530, 594, 595, 711
BmsI GCATC 1 cut(s) 484
Bpu14I TTCGAA 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 2 cut(s) 594, 595
BsaBI GATNNNNATC 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 9 cut(s) 149, 349, 466, 500, 501, 529, 592, 593, 658
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 502, 600
Bse1I ACTGG 2 cut(s) 770, 779
Bse8I GATNNNNATC 1 cut(s) 452
BseBI CCWGG 4 cut(s) 351, 502, 530, 711
BseDI CCNNGG 9 cut(s) 149, 349, 466, 500, 501, 529, 592, 593, 658
BseGI GGATG 3 cut(s) 115, 475, 621
BseJI GATNNNNATC 1 cut(s) 452
BseLI CCNNNNNNNGG 3 cut(s) 448, 502, 600
BseMII CTCAG 1 cut(s) 895
BseNI ACTGG 2 cut(s) 770, 779
BseRI GAGGAG 2 cut(s) 169, 849
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 3 cut(s) 448, 502, 600
BsmAI GTCTC 1 cut(s) 850
BsmFI GGGAC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 98
Bsp143I GATC 5 cut(s) 42, 136, 427, 492, 802
BspACI CCGC 1 cut(s) 825
BspCNI CTCAG 1 cut(s) 894
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 609, 829
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 2 cut(s) 50, 487
BspT104I TTCGAA 1 cut(s) 98
BsrI ACTGG 2 cut(s) 770, 779
BssECI CCNNGG 9 cut(s) 149, 349, 466, 500, 501, 529, 592, 593, 658
BssMI GATC 5 cut(s) 42, 136, 427, 492, 802
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 149, 466, 658
Bst2UI CCWGG 4 cut(s) 351, 502, 530, 711
Bst4CI ACNGT 2 cut(s) 736, 844
Bst6I CTCTTC 2 cut(s) 609, 694
BstACI GRCGYC 1 cut(s) 192
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 339, 586
BstDEI CTNAG 2 cut(s) 849, 881
BstF5I GGATG 3 cut(s) 115, 475, 621
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 5 cut(s) 45, 139, 430, 495, 805
BstMAI GTCTC 1 cut(s) 850
BstMBI GATC 5 cut(s) 42, 136, 427, 492, 802
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 4 cut(s) 351, 502, 530, 711
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 6 cut(s) 349, 500, 528, 592, 593, 709
BstX2I RGATCY 2 cut(s) 42, 802
BstYI RGATCY 2 cut(s) 42, 802
BtgZI GCGATG 1 cut(s) 341
BtrI CACGTC 1 cut(s) 513
BtsCI GGATG 3 cut(s) 115, 475, 621
BtsIMutI CAGTG 1 cut(s) 840
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 339, 586
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 175
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 607
Cfr9I CCCGGG 1 cut(s) 593
Csp6I GTAC 1 cut(s) 16
CviAII CATG 4 cut(s) 13, 176, 573, 811
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 2 cut(s) 849, 881
DpnI GATC 5 cut(s) 44, 138, 429, 494, 804
DpnII GATC 5 cut(s) 42, 136, 427, 492, 802
Eam1104I CTCTTC 2 cut(s) 609, 694
EarI CTCTTC 2 cut(s) 609, 694
Eco130I CCWWGG 3 cut(s) 149, 466, 658
Eco47I GGWCC 1 cut(s) 607
Eco88I CYCGRG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 607
EcoRII CCWGG 4 cut(s) 349, 500, 528, 709
EcoT14I CCWWGG 3 cut(s) 149, 466, 658
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 3 cut(s) 149, 466, 658
FaeI CATG 4 cut(s) 16, 179, 576, 814
FalI AAGNNNNNCTT 2 cut(s) 885, 917
FaqI GGGAC 1 cut(s) 499
FatI CATG 4 cut(s) 12, 175, 572, 810
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 1 cut(s) 826
FokI GGATG 3 cut(s) 122, 462, 608
Fsp4HI GCNGC 1 cut(s) 826
FspBI CTAG 5 cut(s) 150, 467, 482, 557, 919
GlaI GCGC 1 cut(s) 307
GluI GCNGC 1 cut(s) 826
HapII CCGG 1 cut(s) 594
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 16, 179, 576, 814
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HincII GTYRAC 2 cut(s) 163, 190
HindII GTYRAC 2 cut(s) 163, 190
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 1 cut(s) 277
HpaII CCGG 1 cut(s) 594
HphI GGTGA 2 cut(s) 139, 748
Hpy166II GTNNAC 3 cut(s) 163, 190, 763
Hpy188I TCNGA 6 cut(s) 8, 144, 383, 395, 689, 884
Hpy188III TCNNGA 4 cut(s) 176, 274, 445, 806
Hpy8I GTNNAC 3 cut(s) 163, 190, 763
HpyAV CCTTC 5 cut(s) 94, 205, 506, 598, 843
HpyCH4III ACNGT 2 cut(s) 736, 844
HpyCH4IV ACGT 2 cut(s) 192, 512
HpyCH4V TGCA 5 cut(s) 76, 475, 522, 572, 752
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 2 cut(s) 849, 881
HpySE526I ACGT 2 cut(s) 192, 512
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 16, 179, 576, 814
HspAI GCGC 1 cut(s) 306
Kzo9I GATC 5 cut(s) 42, 136, 427, 492, 802
LmnI GCTCC 6 cut(s) 20, 214, 438, 490, 689, 833
LweI GCATC 1 cut(s) 484
MaeI CTAG 5 cut(s) 150, 467, 482, 557, 919
MaeII ACGT 2 cut(s) 192, 512
MaeIII GTNAC 2 cut(s) 248, 409
MalI GATC 5 cut(s) 44, 138, 429, 494, 804
MboI GATC 5 cut(s) 42, 136, 427, 492, 802
MboII GAAGA 8 cut(s) 191, 373, 386, 626, 711, 812, 827, 893
MflI RGATCY 2 cut(s) 42, 802
MluCI AATT 4 cut(s) 309, 779, 792, 874
MmeI TCCRAC 2 cut(s) 66, 87
MnlI CCTC 8 cut(s) 147, 281, 452, 489, 848, 870, 878, 889
Mph1103I ATGCAT 1 cut(s) 574
MseI TTAA 2 cut(s) 227, 567
MspI CCGG 1 cut(s) 594
MspR9I CCNGG 6 cut(s) 351, 502, 530, 594, 595, 711
MvaI CCWGG 4 cut(s) 351, 502, 530, 711
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 2 cut(s) 594, 595
NdeII GATC 5 cut(s) 42, 136, 427, 492, 802
NlaIII CATG 4 cut(s) 16, 179, 576, 814
NlaIV GGNNCC 2 cut(s) 609, 829
NmuCI GTSAC 2 cut(s) 248, 409
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 16
NspV TTCGAA 1 cut(s) 98
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 115
PasI CCCWGGG 1 cut(s) 501
PfeI GAWTC 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 1 cut(s) 827
PpuMI RGGWCCY 1 cut(s) 607
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 4 cut(s) 349, 500, 528, 709
PspGI CCWGG 4 cut(s) 349, 500, 528, 709
PspN4I GGNNCC 2 cut(s) 609, 829
PspPI GGNCC 1 cut(s) 607
PspPPI RGGWCCY 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 2 cut(s) 42, 802
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 227, 567
SatI GCNGC 1 cut(s) 826
Sau3AI GATC 5 cut(s) 42, 136, 427, 492, 802
Sau96I GGNCC 1 cut(s) 607
ScrFI CCNGG 6 cut(s) 351, 502, 530, 594, 595, 711
SfaNI GCATC 1 cut(s) 484
SfuI TTCGAA 1 cut(s) 98
SinI GGWCC 1 cut(s) 607
SmaI CCCGGG 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 4 cut(s) 309, 779, 792, 874
SsiI CCGC 1 cut(s) 825
SspMI CTAG 5 cut(s) 150, 467, 482, 557, 919
StyD4I CCNGG 6 cut(s) 349, 500, 528, 592, 593, 709
StyI CCWWGG 3 cut(s) 149, 466, 658
TaaI ACNGT 2 cut(s) 736, 844
TaiI ACGT 2 cut(s) 195, 515
TaqI TCGA 5 cut(s) 57, 98, 697, 771, 807
TasI AATT 4 cut(s) 309, 779, 792, 874
TauI GCSGC 1 cut(s) 828
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 2 cut(s) 227, 567
Tru9I TTAA 2 cut(s) 227, 567
TscAI CASTG 1 cut(s) 847
TseFI GTSAC 2 cut(s) 248, 409
Tsp45I GTSAC 2 cut(s) 248, 409
TspDTI ATGAA 3 cut(s) 192, 374, 610
TspGWI ACGGA 2 cut(s) 24, 33
TspMI CCCGGG 1 cut(s) 593
TspRI CASTG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 607
XapI RAATTY 1 cut(s) 874
XceI RCATGY 1 cut(s) 16
XmaI CCCGGG 1 cut(s) 593
XmaJI CCTAGG 2 cut(s) 149, 466
XmiI GTMKAC 1 cut(s) 762
XspI CTAG 5 cut(s) 150, 467, 482, 557, 919
ZraI GACGTC 1 cut(s) 193
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.