Rh4AG316100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000354
Physical Location & Seq
Reverse (-)
62664356 .. 62666846
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG316100.1

Sequence Viewer

Length: 1539 bp
ATGTCTCTCAACACCCTACCGCTAACCTTATGGCTTCCATTTCTCCTTCTTCTCCCTCTACTGATTTTACTGAAAAAGAATAAAAAGCCACAAAAACACTTCCCGCCAAGCCCTCCTAAGCTTCCCATCATAGGCAACTTACACCAATTTGCACAATCTGGTTCATCACCTCACCAAAATCTATGGCGCCTCTCGAAAAAGTTCGGTCCAATCATGTTCCTACACCTCGGTCGCATACCGACCCTCATCATCTCATCAGCTGAAGCAGCAAAACAGGTCTTGAAAGATAATGATCTCAACTGCTGCAGCAGACCCTCCTCCTCGGGGTCTCGAAAACTCACCTACAACTATATAGACATGGGATTTGCGCCTTATGGTGAGTATTGGCGAGAAATGAGAAAACTATGTGTCCTTGAGCTTTTCAGTGTGAAAAGGGTGCAGTCTTATCGGTTGATTAGGGAAGAAGAAGTGGCTAAAATGATTGACTCAATATCCAAAGAATCTGCATCTTCGTCCTCTAATTCATGTGTTAATATTACTGAGAAGTTGTATGCTGTCATGGGCAGTATAACTTTCAGGGTTATTTTCGGGACAAGTTTCGAGGGAAGTGATTTCGAGCACGATAGGTTTCATCAAGTGCTTCATGAAGCTGAAATCATGCTTGCAGGCTTCTCTGCAGCTGATTATTTTCCATTTGGTTATCTTATAGACAGGATCTCCGGGAAACATAAACAATTTGAAAGGGTATCCGGGGAACTTCATCACTTTTTTCAGCAGGTGATTGATGATCATCTCAAGCCAGGGAGGACACAACCAGAGCATGAAGACATAGTTGATGTGTTGCTTAAAATAGTGAAGGAGCAAACTGGTTTTGGAGCTGCTCATTTTGGTCATGATAACATCAAGGCACTTCTTGTGAACTTATTTCTAGGTGGAATAGATACTGGTGCAATTACCATGCTGTGGGCGATGGCTGAGCTAGCTAAGAATCCTAGACTGATGAAGAAAGCACAGGATGAAGTTAGAAGCTGCGTTGGAAACAAAGGAAAAGTCTCTGAAAGTGATACGGAACAGCTTCCGTACCTCAAGATGATAATCAAAGAAACACTTCGACTGCATCCTCCAGCTCCACTGATGCTTCCAAGAGAGTCCATGTCCCCCTTTAAAGTCCTTGGGTATGATGTTGAACCCAAAACACTCGTCATAATTAATGACTGGGCAATTTCACGAGATCCAGAATTTTGGAAAGACCCAGAAGAGTTCATCCCAGAAAGGTTTGATGATAGCTCTGTCGATTTTAGGGGGCAACACTTTGAGTTCTTGCCATTTGGAGCTGGTCGGAGAATGTGTCCGGGAATTTACATGGCAACAACAACTGTGGAGCTTGGACTTGCAAATCTGCTGTACAGCTTTGATTGGAAATCGCCAGAGGGAATGAAAGAAGCTGATATCAACATGGAAGAAACAACTGGGGAACTTTCCCTAACTATCTCCAAGAAAACTGCTCTCAACCTTGTCCCCGTGAAGTTTTCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

512

Amino Acids

58.06

Weight (kDa)

6.98

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 492 6.4e-101 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 766
Acc36I ACCTGC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 963
AciI CCGC 2 cut(s) 20, 104
AclWI GGATC 2 cut(s) 722, 1226
AcsI RAATTY 2 cut(s) 1238, 1356
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 2 cut(s) 1082, 1406
AfiI CCNNNNNNNGG 3 cut(s) 131, 324, 963
AgsI TTSAA 3 cut(s) 283, 740, 1187
AjnI CCWGG 1 cut(s) 799
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 3 cut(s) 9, 333, 1057
AlwI GGATC 2 cut(s) 722, 1226
Ama87I CYCGRG 1 cut(s) 322
ApeKI GCWGC 6 cut(s) 266, 303, 306, 677, 878, 1029
ApoI RAATTY 2 cut(s) 1238, 1356
ArsI GACNNNNNNTTYG 6 cut(s) 347, 379, 1035, 1067, 1185, 1217
AseI ATTAAT 1 cut(s) 1209
Asp700I GAANNNNTTC 3 cut(s) 200, 1074, 1107
AspLEI GCGC 2 cut(s) 189, 370
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 3 cut(s) 721, 751, 1353
AsuHPI GGTGA 5 cut(s) 159, 164, 331, 389, 790
AsuNHI GCTAGC 1 cut(s) 979
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 186
BauI CACGAG 1 cut(s) 1227
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 6 cut(s) 278, 290, 318, 689, 865, 1016
BccI CCATC 2 cut(s) 134, 964
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 801
BciVI GTATCC 1 cut(s) 757
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 3 cut(s) 721, 751, 1353
BcoDI GTCTC 3 cut(s) 9, 333, 1057
BfaI CTAG 3 cut(s) 929, 980, 993
BfmI CTRYAG 2 cut(s) 304, 675
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 766
BfuI GTATCC 1 cut(s) 757
BisI GCNGC 6 cut(s) 267, 304, 307, 678, 879, 1030
BlpI GCTNAGC 1 cut(s) 975
BlsI GCNGC 6 cut(s) 268, 305, 308, 679, 880, 1031
Bme1390I CCNGG 4 cut(s) 721, 751, 801, 1353
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 4 cut(s) 721, 751, 801, 1353
BmrI ACTGGG 2 cut(s) 1225, 1479
BmsI GCATC 3 cut(s) 515, 1125, 1126
BmtI GCTAGC 1 cut(s) 983
BmuI ACTGGG 2 cut(s) 1225, 1479
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 975
BpuEI CTTGAG 3 cut(s) 434, 779, 1070
BpuMI CCSGG 3 cut(s) 721, 751, 1353
BsaBI GATNNNNATC 3 cut(s) 291, 789, 1094
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 5 cut(s) 226, 321, 750, 800, 1171
BsaXI ACNNNNNCTCC 4 cut(s) 701, 731, 1333, 1363
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 324, 963
Bse1I ACTGG 4 cut(s) 871, 949, 1220, 1474
Bse8I GATNNNNATC 3 cut(s) 291, 789, 1094
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 5 cut(s) 226, 321, 750, 800, 1171
BseGI GGATG 3 cut(s) 1021, 1117, 1263
BseJI GATNNNNATC 3 cut(s) 291, 789, 1094
BseLI CCNNNNNNNGG 3 cut(s) 131, 324, 963
BseMII CTCAG 2 cut(s) 531, 966
BseNI ACTGG 4 cut(s) 871, 949, 1220, 1474
BseRI GAGGAG 2 cut(s) 307, 310
BseXI GCAGC 6 cut(s) 278, 290, 318, 689, 865, 1016
BsgI GTGCAG 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 720, 750, 1352
BslFI GGGAC 3 cut(s) 604, 1141, 1502
BslI CCNNNNNNNGG 3 cut(s) 131, 324, 963
BsmAI GTCTC 3 cut(s) 9, 333, 1057
BsmFI GGGAC 3 cut(s) 604, 1141, 1502
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 621
Bsp1407I TGTACA 1 cut(s) 1404
Bsp143I GATC 4 cut(s) 292, 714, 787, 1231
Bsp1720I GCTNAGC 1 cut(s) 975
BspACI CCGC 2 cut(s) 20, 104
BspCNI CTCAG 2 cut(s) 532, 967
BspHI TCATGA 2 cut(s) 643, 892
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 2 cut(s) 308, 679
BspMI ACCTGC 1 cut(s) 766
BspOI GCTAGC 1 cut(s) 983
BspPI GGATC 2 cut(s) 722, 1226
BspT107I GGYRCC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 1404
BsrI ACTGG 4 cut(s) 871, 949, 1220, 1474
BssECI CCNNGG 5 cut(s) 226, 321, 750, 800, 1171
BssMI GATC 4 cut(s) 292, 714, 787, 1231
BssNI GRCGYC 1 cut(s) 187
BssSI CACGAG 1 cut(s) 1227
BssT1I CCWWGG 1 cut(s) 1171
Bst2BI CACGAG 1 cut(s) 1227
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 1 cut(s) 1378
Bst6I CTCTTC 1 cut(s) 1251
BstACI GRCGYC 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 1404
BstC8I GCNNGC 3 cut(s) 663, 667, 981
BstDEI CTNAG 4 cut(s) 117, 540, 975, 984
BstF5I GGATG 3 cut(s) 1021, 1117, 1263
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 189, 370
BstKTI GATC 4 cut(s) 295, 717, 790, 1234
BstMAI GTCTC 3 cut(s) 9, 333, 1057
BstMBI GATC 4 cut(s) 292, 714, 787, 1231
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 2 cut(s) 266, 980
BstNI CCWGG 1 cut(s) 801
BstSCI CCNGG 4 cut(s) 719, 749, 799, 1351
BstSFI CTRYAG 2 cut(s) 304, 675
BstV1I GCAGC 6 cut(s) 278, 290, 318, 689, 865, 1016
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 2 cut(s) 714, 1231
BstXI CCANNNNNNTGG 1 cut(s) 1242
BstYI RGATCY 2 cut(s) 714, 1231
BsuI GTATCC 1 cut(s) 757
BtgZI GCGATG 1 cut(s) 983
BtsCI GGATG 3 cut(s) 1021, 1117, 1263
BtsIMutI CAGTG 2 cut(s) 430, 1130
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 3 cut(s) 663, 667, 981
CciI TCATGA 2 cut(s) 643, 892
CfoI GCGC 2 cut(s) 189, 370
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 1081, 1405
CspCI CAANNNNNGTGG 2 cut(s) 1359, 1394
CviQI GTAC 2 cut(s) 1081, 1405
DdeI CTNAG 4 cut(s) 117, 540, 975, 984
DinI GGCGCC 1 cut(s) 188
DpnI GATC 4 cut(s) 294, 716, 789, 1233
DpnII GATC 4 cut(s) 292, 714, 787, 1231
DraI TTTAAA 1 cut(s) 1165
Eam1104I CTCTTC 1 cut(s) 1251
EarI CTCTTC 1 cut(s) 1251
Eco130I CCWWGG 1 cut(s) 1171
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 1450
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 1 cut(s) 1450
EcoT14I CCWWGG 1 cut(s) 1171
EgeI GGCGCC 1 cut(s) 188
EheI GGCGCC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 1171
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FaqI GGGAC 3 cut(s) 604, 1141, 1502
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 6 cut(s) 267, 304, 307, 678, 879, 1030
FokI GGATG 3 cut(s) 1028, 1104, 1250
Fsp4HI GCNGC 6 cut(s) 267, 304, 307, 678, 879, 1030
FspBI CTAG 3 cut(s) 929, 980, 993
GlaI GCGC 2 cut(s) 188, 369
GluI GCNGC 6 cut(s) 267, 304, 307, 678, 879, 1030
GsuI CTGGAG 1 cut(s) 1107
HaeII RGCGCY 1 cut(s) 190
HapII CCGG 3 cut(s) 720, 750, 1352
HhaI GCGC 2 cut(s) 189, 370
Hin1I GRCGYC 1 cut(s) 187
Hin6I GCGC 2 cut(s) 187, 368
HinP1I GCGC 2 cut(s) 187, 368
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 4 cut(s) 485, 500, 988, 1148
HpaII CCGG 3 cut(s) 720, 750, 1352
HphI GGTGA 5 cut(s) 159, 164, 331, 389, 790
Hpy166II GTNNAC 1 cut(s) 919
Hpy188I TCNGA 2 cut(s) 1057, 1341
Hpy188III TCNNGA 9 cut(s) 193, 280, 330, 589, 644, 893, 1087, 1227, 1235
Hpy8I GTNNAC 1 cut(s) 919
HpyAV CCTTC 2 cut(s) 56, 850
HpyCH4III ACNGT 1 cut(s) 1378
HpyCH4V TGCA 9 cut(s) 152, 306, 439, 506, 665, 677, 950, 1117, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 980
HpyF3I CTNAG 4 cut(s) 117, 540, 975, 984
Hsp92I GRCGYC 1 cut(s) 187
HspAI GCGC 2 cut(s) 187, 368
KasI GGCGCC 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 4 cut(s) 292, 714, 787, 1231
LmnI GCTCC 5 cut(s) 859, 875, 1132, 1331, 1381
Lsp1109I GCAGC 6 cut(s) 278, 290, 318, 689, 865, 1016
LweI GCATC 3 cut(s) 515, 1125, 1126
MaeI CTAG 3 cut(s) 929, 980, 993
MalI GATC 4 cut(s) 294, 716, 789, 1233
MboI GATC 4 cut(s) 292, 714, 787, 1231
MboII GAAGA 8 cut(s) 41, 473, 476, 501, 836, 1015, 1268, 1472
MflI RGATCY 2 cut(s) 714, 1231
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 8 cut(s) 146, 520, 734, 951, 1206, 1221, 1238, 1356
Mly113I GGCGCC 1 cut(s) 187
MlyI GAGTC 2 cut(s) 479, 1157
MmeI TCCRAC 2 cut(s) 1015, 1319
MroXI GAANNNNTTC 3 cut(s) 200, 1074, 1107
MseI TTAA 4 cut(s) 531, 846, 1164, 1209
MspA1I CMGCKG 2 cut(s) 260, 680
MspI CCGG 3 cut(s) 720, 750, 1352
MspR9I CCNGG 4 cut(s) 721, 751, 801, 1353
MvaI CCWGG 1 cut(s) 801
MwoI GCNNNNNNNGC 2 cut(s) 266, 980
NarI GGCGCC 1 cut(s) 187
NciI CCSGG 3 cut(s) 721, 751, 1353
NdeII GATC 4 cut(s) 292, 714, 787, 1231
NheI GCTAGC 1 cut(s) 979
NlaIV GGNNCC 1 cut(s) 188
PagI TCATGA 2 cut(s) 643, 892
PaqCI CACCTGC 1 cut(s) 766
PdmI GAANNNNTTC 3 cut(s) 200, 1074, 1107
PfeI GAWTC 2 cut(s) 500, 988
PflMI CCANNNNNTGG 1 cut(s) 963
PfoI TCCNGGA 2 cut(s) 719, 1351
PkrI GCNGC 6 cut(s) 268, 305, 308, 679, 880, 1031
PleI GAGTC 2 cut(s) 479, 1156
PluTI GGCGCC 1 cut(s) 190
PpsI GAGTC 2 cut(s) 479, 1156
PshBI ATTAAT 1 cut(s) 1209
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 2 cut(s) 308, 679
PsuI RGATCY 2 cut(s) 714, 1231
PvuII CAGCTG 2 cut(s) 260, 680
RsaI GTAC 2 cut(s) 1082, 1406
RsaNI GTAC 2 cut(s) 1081, 1405
SaqAI TTAA 4 cut(s) 531, 846, 1164, 1209
SatI GCNGC 6 cut(s) 267, 304, 307, 678, 879, 1030
Sau3AI GATC 4 cut(s) 292, 714, 787, 1231
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 2 cut(s) 479, 1157
ScrFI CCNGG 4 cut(s) 721, 751, 801, 1353
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 3 cut(s) 515, 1125, 1126
SfcI CTRYAG 2 cut(s) 304, 675
SfoI GGCGCC 1 cut(s) 188
SinI GGWCC 1 cut(s) 206
SmlI CTYRAG 3 cut(s) 413, 794, 1085
SmoI CTYRAG 3 cut(s) 413, 794, 1085
Sse9I AATT 8 cut(s) 146, 520, 734, 951, 1206, 1221, 1238, 1356
SsiI CCGC 2 cut(s) 20, 104
SspDI GGCGCC 1 cut(s) 186
SspI AATATT 1 cut(s) 535
SspMI CTAG 3 cut(s) 929, 980, 993
StyD4I CCNGG 4 cut(s) 719, 749, 799, 1351
StyI CCWWGG 1 cut(s) 1171
TaaI ACNGT 1 cut(s) 1378
TaqI TCGA 6 cut(s) 194, 331, 600, 615, 1111, 1293
TaqII GACCGA 2 cut(s) 194, 218
TasI AATT 8 cut(s) 146, 520, 734, 951, 1206, 1221, 1238, 1356
TatI WGTACW 1 cut(s) 1404
TfiI GAWTC 2 cut(s) 500, 988
Tru1I TTAA 4 cut(s) 531, 846, 1164, 1209
Tru9I TTAA 4 cut(s) 531, 846, 1164, 1209
TscAI CASTG 2 cut(s) 430, 1137
TseI GCWGC 6 cut(s) 266, 303, 306, 677, 878, 1029
TspGWI ACGGA 2 cut(s) 1068, 1082
TspRI CASTG 2 cut(s) 430, 1137
Van91I CCANNNNNTGG 1 cut(s) 963
VpaK11BI GGWCC 1 cut(s) 206
VspI ATTAAT 1 cut(s) 1209
XapI RAATTY 2 cut(s) 1238, 1356
XmnI GAANNNNTTC 3 cut(s) 200, 1074, 1107
XspI CTAG 3 cut(s) 929, 980, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.