RchiOBHm_Chr4g0419491

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000354
Physical Location & Seq
Reverse (-)
44938192 .. 44939826
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38939

Sequence Viewer

Length: 864 bp
ATGATTGATTCAATCTCTGACGCATCAGCTTCATCTTCTCCTGTTAACCTTACTGAGAAGTTGTTTGCTCTCATGAGCAGTATAATTTTCAGGATTGTTTTCGGGACAAGTTTCCAAGGGAGCGATTTGGAGCATGAAAGATTTCTTCGAGTGCTCCTTGATAGATCTCCGGTAAACATAGAGAGTTTGACAGGGTTTCCAGGGAAGTTCATCGCTTTTTTCAGCAACATTGATGATCATCTCAGGCCTGGGAGGACACAACAGGAGCATGAAGATATAGTTGATGTGCTGCTTAAAATGGTCAAGACGCAAACTGAGATTGGAGCTGCTCAGCTTGGTCATAATAACATCAATGCAGTTCTCTTGGATTTGTTTTTAGGAGGAGTAGACACCACTGCAACTGCCATGGTTTGGGCAATGGCAGAGCTAGCTACGAATATAAGAGTGATGAAGAAAGCACAGGAAGAAATTAGAAACTGCATTGGAAATAAAGGAAAAGCCACTGAAATTGATACAGAGCAGCTGCAGTACCTCAAGATGATAGTGAAAGAAACACTTCGACAACATCCATCAGGTCCCCTACTTCTTCCAAGAGAAACCATGTCCCACTTCAAAGTCCTCAGCAATGATGTTGACCCCAAAACAACAATCCTTGTTAATGATTGGGCAATCTCACGAGATCCAGAATTTTGGAACAACCCAGAAACGTTCATTCCGGAAAGATTTGATGATAGGTCTGTTGATTTTAAAGGGCAACACTTTGACTTCTTGCCGTTTGGAGCTGGTCGAAGAATCTGTCCAGGGATTTTATATGGCAGCGACGGCAATGGAGCTTGTACTTGCCAAATTGTTGTGCTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

287

Amino Acids

32.07

Weight (kDa)

5.3

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 268 7.1e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 411
AccI GTMKAC 1 cut(s) 387
AccIII TCCGGA 1 cut(s) 715
AclI AACGTT 1 cut(s) 707
AclWI GGATC 1 cut(s) 674
AcsI RAATTY 1 cut(s) 686
AfaI GTAC 2 cut(s) 530, 838
AfiI CCNNNNNNNGG 1 cut(s) 411
AgsI TTSAA 2 cut(s) 12, 613
AjnI CCWGG 3 cut(s) 199, 247, 799
AluBI AGCT 8 cut(s) 29, 326, 334, 427, 431, 523, 782, 833
AluI AGCT 8 cut(s) 29, 326, 334, 427, 431, 523, 782, 833
Alw21I GWGCWC 1 cut(s) 156
AlwI GGATC 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 6 cut(s) 289, 326, 520, 523, 816, 856
ApoI RAATTY 1 cut(s) 686
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
Asp700I GAANNNNTTC 2 cut(s) 141, 555
AspS9I GGNCC 1 cut(s) 575
AsuNHI GCTAGC 1 cut(s) 427
AvaII GGWCC 1 cut(s) 575
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 620
BbvI GCAGC 6 cut(s) 276, 313, 510, 532, 828, 843
BccI CCATC 1 cut(s) 577
BceAI ACGGC 2 cut(s) 757, 838
BciT130I CCWGG 3 cut(s) 201, 249, 801
BclI TGATCA 1 cut(s) 235
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 524
BglII AGATCT 1 cut(s) 164
BisI GCNGC 6 cut(s) 290, 327, 521, 524, 817, 857
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 6 cut(s) 291, 328, 522, 525, 818, 858
Bme1390I CCNGG 3 cut(s) 201, 249, 801
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 3 cut(s) 201, 249, 801
BmsI GCATC 1 cut(s) 32
BmtI GCTAGC 1 cut(s) 431
Bpu10I CCTNAGC 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 237
BsaJI CCNNGG 5 cut(s) 115, 200, 248, 405, 800
BsaWI WCCGGW 2 cut(s) 169, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse3DI GCAATG 3 cut(s) 423, 631, 832
Bse8I GATNNNNATC 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 3 cut(s) 201, 249, 801
BseDI CCNNGG 5 cut(s) 115, 200, 248, 405, 800
BseGI GGATG 1 cut(s) 565
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMI GCAATG 3 cut(s) 423, 631, 832
BseMII CTCAG 5 cut(s) 45, 256, 306, 344, 634
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 6 cut(s) 276, 313, 510, 532, 828, 843
BshFI GGCC 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 2 cut(s) 170, 716
BslFI GGGAC 3 cut(s) 118, 561, 589
BslI CCNNNNNNNGG 1 cut(s) 411
BsmFI GGGAC 3 cut(s) 118, 561, 589
BsnI GGCC 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 715
Bsp143I GATC 3 cut(s) 164, 235, 679
Bsp1720I GCTNAGC 1 cut(s) 330
Bsp19I CCATGG 1 cut(s) 405
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 5 cut(s) 46, 255, 307, 343, 633
BspEI TCCGGA 1 cut(s) 715
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 577
BspMAI CTGCAG 1 cut(s) 528
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 1 cut(s) 674
BsrDI GCAATG 3 cut(s) 423, 631, 832
BssECI CCNNGG 5 cut(s) 115, 200, 248, 405, 800
BssMI GATC 3 cut(s) 164, 235, 679
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 2 cut(s) 115, 405
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 3 cut(s) 201, 249, 801
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 5 cut(s) 54, 242, 315, 330, 620
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 565
BstKTI GATC 3 cut(s) 167, 238, 682
BstMBI GATC 3 cut(s) 164, 235, 679
BstMWI GCNNNNNNNGC 2 cut(s) 428, 822
BstNI CCWGG 3 cut(s) 201, 249, 801
BstSCI CCNGG 3 cut(s) 199, 247, 799
BstSFI CTRYAG 1 cut(s) 524
BstV1I GCAGC 6 cut(s) 276, 313, 510, 532, 828, 843
BstX2I RGATCY 2 cut(s) 164, 679
BstXI CCANNNNNNTGG 1 cut(s) 690
BstYI RGATCY 2 cut(s) 164, 679
BsuRI GGCC 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 565
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 393, 501
Cac8I GCNNGC 1 cut(s) 429
CciI TCATGA 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 2 cut(s) 29, 316
Csp6I GTAC 2 cut(s) 529, 837
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 5 cut(s) 73, 134, 269, 406, 601
CviQI GTAC 2 cut(s) 529, 837
DdeI CTNAG 5 cut(s) 54, 242, 315, 330, 620
DpnI GATC 3 cut(s) 166, 237, 681
DpnII GATC 3 cut(s) 164, 235, 679
DraI TTTAAA 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 115, 405
Eco147I AGGCCT 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 199, 247, 799
EcoT14I CCWWGG 2 cut(s) 115, 405
ErhI CCWWGG 2 cut(s) 115, 405
FaeI CATG 5 cut(s) 76, 137, 272, 409, 604
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 3 cut(s) 118, 561, 589
FatI CATG 5 cut(s) 72, 133, 268, 405, 600
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 6 cut(s) 290, 327, 521, 524, 817, 857
FokI GGATG 1 cut(s) 552
Fsp4HI GCNGC 6 cut(s) 290, 327, 521, 524, 817, 857
FspBI CTAG 1 cut(s) 428
GluI GCNGC 6 cut(s) 290, 327, 521, 524, 817, 857
HaeIII GGCC 1 cut(s) 247
HapII CCGG 2 cut(s) 170, 716
HgaI GACGC 2 cut(s) 29, 316
Hin1II CATG 5 cut(s) 76, 137, 272, 409, 604
HincII GTYRAC 2 cut(s) 46, 634
HindII GTYRAC 2 cut(s) 46, 634
HinfI GANTC 2 cut(s) 8, 792
HpaI GTTAAC 1 cut(s) 46
HpaII CCGG 2 cut(s) 170, 716
Hpy166II GTNNAC 4 cut(s) 46, 175, 388, 634
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 8 cut(s) 73, 91, 103, 304, 535, 675, 683, 716
Hpy8I GTNNAC 4 cut(s) 46, 175, 388, 634
Hpy99I CGWCG 1 cut(s) 824
HpyCH4IV ACGT 1 cut(s) 707
HpyCH4V TGCA 4 cut(s) 356, 398, 480, 526
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 822
HpyF3I CTNAG 5 cut(s) 54, 242, 315, 330, 620
HpySE526I ACGT 1 cut(s) 707
Hsp92II CATG 5 cut(s) 76, 137, 272, 409, 604
Kpn2I TCCGGA 1 cut(s) 715
Ksp22I TGATCA 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 46
Kzo9I GATC 3 cut(s) 164, 235, 679
LmnI GCTCC 7 cut(s) 120, 130, 159, 265, 323, 779, 830
Lsp1109I GCAGC 6 cut(s) 276, 313, 510, 532, 828, 843
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 707
MalI GATC 3 cut(s) 166, 237, 681
MboI GATC 3 cut(s) 164, 235, 679
MboII GAAGA 7 cut(s) 27, 137, 284, 463, 476, 578, 801
MflI RGATCY 2 cut(s) 164, 679
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 5 cut(s) 84, 468, 507, 686, 846
MnlI CCTC 4 cut(s) 246, 374, 542, 629
MroI TCCGGA 1 cut(s) 715
MroXI GAANNNNTTC 2 cut(s) 141, 555
MseI TTAA 4 cut(s) 45, 294, 657, 747
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 2 cut(s) 170, 716
MspR9I CCNGG 3 cut(s) 201, 249, 801
MvaI CCWGG 3 cut(s) 201, 249, 801
MwoI GCNNNNNNNGC 2 cut(s) 428, 822
NcoI CCATGG 1 cut(s) 405
NdeII GATC 3 cut(s) 164, 235, 679
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 5 cut(s) 76, 137, 272, 409, 604
NlaIV GGNNCC 1 cut(s) 577
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 247
PdmI GAANNNNTTC 2 cut(s) 141, 555
PfeI GAWTC 2 cut(s) 8, 792
PflMI CCANNNNNTGG 1 cut(s) 411
PkrI GCNGC 6 cut(s) 291, 328, 522, 525, 818, 858
PpuMI RGGWCCY 1 cut(s) 575
Psp1406I AACGTT 1 cut(s) 707
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 3 cut(s) 199, 247, 799
PspGI CCWGG 3 cut(s) 199, 247, 799
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 1 cut(s) 575
PspPPI RGGWCCY 1 cut(s) 575
PstI CTGCAG 1 cut(s) 528
PsuI RGATCY 2 cut(s) 164, 679
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 2 cut(s) 530, 838
RsaNI GTAC 2 cut(s) 529, 837
SaqAI TTAA 4 cut(s) 45, 294, 657, 747
SatI GCNGC 6 cut(s) 290, 327, 521, 524, 817, 857
Sau3AI GATC 3 cut(s) 164, 235, 679
Sau96I GGNCC 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 201, 249, 801
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 524
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 5 cut(s) 84, 468, 507, 686, 846
SseBI AGGCCT 1 cut(s) 247
SspMI CTAG 1 cut(s) 428
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 3 cut(s) 199, 247, 799
StyI CCWWGG 2 cut(s) 115, 405
TaiI ACGT 1 cut(s) 710
TaqI TCGA 3 cut(s) 148, 559, 787
TasI AATT 5 cut(s) 84, 468, 507, 686, 846
TatI WGTACW 1 cut(s) 836
TfiI GAWTC 2 cut(s) 8, 792
Tru1I TTAA 4 cut(s) 45, 294, 657, 747
Tru9I TTAA 4 cut(s) 45, 294, 657, 747
TscAI CASTG 2 cut(s) 400, 508
TseI GCWGC 6 cut(s) 289, 326, 520, 523, 816, 856
TspDTI ATGAA 6 cut(s) 21, 150, 199, 285, 464, 700
TspRI CASTG 2 cut(s) 400, 508
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 1 cut(s) 686
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 2 cut(s) 141, 555
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.