AT5G52480

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000586
Physical Location & Seq
Reverse (-)
21296833 .. 21297858
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52480.2

Sequence Viewer

Length: 726 bp
ATGCGAATCTTGAGTTACAACAAACCAGTACTGGTGAACAATCCTGAGGCGATATGTCGTAACGCCGTTGATCGTAGCGAGGGTGGCTTGCTGGAGATCAACATTGGGGACTTCGGGAGTGATTCTCTCCTTACATACATCGCGGAGAGGTCAAGTAATCTTAGAAGCCTTAGACTAATGTGCTCAGAAATAACAGACGATGGATTTGTTCAAGCAGTTGTGAAACTTCCAATGCTTGAAGAGCTTGAGGTTTCAGGAATATCATTGTCAGGAGAGTCTATGAAACTTGCAGGGCTGTCTTGTCCAAATCTGAAGACATTGATGCTAAACCGCCTCTTTTACTTAAGTTCGGATGATGATGATCATGATGCCATAGCAATTGCAGAAAGCATGCCTAAACTACGCCATCTCCAGCTTTGTGGGAACAAACTAACCAAAACAGGCTTAAACGCCATTCTTGACGGTTGTCCTCACCTGGAACACCTCGATTTACGCCAATGTATCAACCTTGTCGGGAATCTAGAGAAGCGTTGTTTTGAGAAAATTAAAGATTTGAGACGCCACAATGATTCTACCTCTGCTGATTACCCGTTTTCTTTTGAAACTTACTATTTTCATTGGTCACGCCAATTGGCAATACTTAAAAATGCGCTACCGATGGAAGAAGGCGCCTATAACTTTGATCCAATAGTGGAAGAAGCCGAATATTACTTGAAACTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

241

Amino Acids

27.29

Weight (kDa)

5.11

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 83 - 136 1.1e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 668
AccII CGCG 1 cut(s) 143
AciI CCGC 2 cut(s) 143, 331
AclWI GGATC 1 cut(s) 677
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 2 cut(s) 559, 669
AfaI GTAC 1 cut(s) 30
AflII CTTAAG 1 cut(s) 343
AgsI TTSAA 4 cut(s) 212, 239, 602, 715
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 2 cut(s) 244, 415
AluI AGCT 2 cut(s) 244, 415
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 550
AlwI GGATC 1 cut(s) 677
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AspLEI GCGC 2 cut(s) 652, 671
AsuHPI GGTGA 2 cut(s) 46, 464
AxyI CCTNAGG 1 cut(s) 45
BanI GGYRCC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 185
BccI CCATC 3 cut(s) 194, 414, 652
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 550
BfaI CTAG 1 cut(s) 521
BfoI RGCGCY 1 cut(s) 672
BfrI CTTAAG 1 cut(s) 343
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 476
BmiI GGNNCC 1 cut(s) 670
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 2 cut(s) 312, 358
BoxI GACNNNNGTC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 320
BplI GAGNNNNNCTC 2 cut(s) 109, 141
BpmI CTGGAG 2 cut(s) 113, 395
BpuEI CTTGAG 2 cut(s) 31, 266
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 559, 669
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bse1I ACTGG 2 cut(s) 26, 36
Bse21I CCTNAGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 1 cut(s) 476
BseGI GGATG 1 cut(s) 358
BseJI GATNNNNATC 1 cut(s) 360
BseMII CTCAG 2 cut(s) 36, 198
BseNI ACTGG 2 cut(s) 26, 36
Bsh1236I CGCG 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 185
BslFI GGGAC 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 550
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 4 cut(s) 70, 96, 361, 682
BspACI CCGC 2 cut(s) 143, 331
BspCNI CTCAG 2 cut(s) 37, 197
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 670
BspPI GGATC 1 cut(s) 677
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 668
BspTI CTTAAG 1 cut(s) 343
BsrI ACTGG 2 cut(s) 26, 36
BssMI GATC 4 cut(s) 70, 96, 361, 682
BssNI GRCGYC 2 cut(s) 559, 669
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 464, 720
Bst6I CTCTTC 1 cut(s) 234
BstACI GRCGYC 2 cut(s) 559, 669
BstAFI CTTAAG 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 89, 392
BstDEI CTNAG 4 cut(s) 45, 161, 170, 184
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 2 cut(s) 652, 671
BstKTI GATC 4 cut(s) 73, 99, 364, 685
BstMAI GTCTC 1 cut(s) 550
BstMBI GATC 4 cut(s) 70, 96, 361, 682
BstMWI GCNNNNNNNGC 2 cut(s) 84, 241
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 394
BstPAI GACNNNNGTC 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 474
BstUI CGCG 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 419
Bsu36I CCTNAGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 89, 392
CciI TCATGA 1 cut(s) 364
CfoI GCGC 2 cut(s) 652, 671
CseI GACGC 1 cut(s) 567
Csp6I GTAC 1 cut(s) 29
CviAII CATG 2 cut(s) 365, 391
CviJI RGCY 7 cut(s) 87, 168, 244, 295, 415, 444, 701
CviKI_1 RGCY 7 cut(s) 87, 168, 244, 295, 415, 444, 701
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 4 cut(s) 45, 161, 170, 184
DinI GGCGCC 1 cut(s) 670
DpnI GATC 4 cut(s) 72, 98, 363, 684
DpnII GATC 4 cut(s) 70, 96, 361, 682
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 332
Eco81I CCTNAGG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 670
EheI GGCGCC 1 cut(s) 670
Esp3I CGTCTC 1 cut(s) 550
FaeI CATG 2 cut(s) 368, 394
FaiI YATR 7 cut(s) 55, 136, 281, 366, 374, 392, 675
FaqI GGGAC 1 cut(s) 122
FatI CATG 2 cut(s) 364, 390
FbaI TGATCA 1 cut(s) 361
FokI GGATG 1 cut(s) 365
FspBI CTAG 1 cut(s) 521
GlaI GCGC 2 cut(s) 651, 670
GsuI CTGGAG 2 cut(s) 113, 395
HaeII RGCGCY 1 cut(s) 672
HgaI GACGC 1 cut(s) 567
HhaI GCGC 2 cut(s) 652, 671
Hin1I GRCGYC 2 cut(s) 559, 669
Hin1II CATG 2 cut(s) 368, 394
Hin6I GCGC 2 cut(s) 650, 669
HinP1I GCGC 2 cut(s) 650, 669
HinfI GANTC 5 cut(s) 6, 122, 275, 517, 569
HphI GGTGA 2 cut(s) 46, 464
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 187, 312, 352
Hpy188III TCNNGA 9 cut(s) 10, 44, 115, 255, 270, 365, 458, 514, 521
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 2 cut(s) 464, 720
HpyCH4V TGCA 2 cut(s) 290, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 241
HpyF3I CTNAG 4 cut(s) 45, 161, 170, 184
Hsp92I GRCGYC 2 cut(s) 559, 669
Hsp92II CATG 2 cut(s) 368, 394
HspAI GCGC 2 cut(s) 650, 669
KasI GGCGCC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 4 cut(s) 70, 96, 361, 682
LguI GCTCTTC 1 cut(s) 234
LweI GCATC 2 cut(s) 312, 358
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 3 cut(s) 14, 59, 621
MalI GATC 4 cut(s) 72, 98, 363, 684
MboI GATC 4 cut(s) 70, 96, 361, 682
MboII GAAGA 4 cut(s) 251, 325, 674, 707
MfeI CAATTG 2 cut(s) 378, 629
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 3 cut(s) 378, 543, 629
Mly113I GGCGCC 1 cut(s) 669
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 8 cut(s) 40, 73, 141, 241, 344, 480, 494, 586
MseI TTAA 4 cut(s) 344, 446, 546, 642
MspCI CTTAAG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 476
MunI CAATTG 2 cut(s) 378, 629
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 84, 241
NarI GGCGCC 1 cut(s) 669
NdeII GATC 4 cut(s) 70, 96, 361, 682
NlaIII CATG 2 cut(s) 368, 394
NlaIV GGNNCC 1 cut(s) 670
NmuCI GTSAC 1 cut(s) 621
NspI RCATGY 1 cut(s) 394
PaeI GCATGC 1 cut(s) 394
PagI TCATGA 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 234
PfeI GAWTC 4 cut(s) 6, 122, 517, 569
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 672
PpsI GAGTC 1 cut(s) 283
PshAI GACNNNNGTC 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 670
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 4 cut(s) 344, 446, 546, 642
Sau3AI GATC 4 cut(s) 70, 96, 361, 682
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 1 cut(s) 185
SetI ASST 8 cut(s) 152, 246, 252, 417, 477, 486, 510, 578
SfaNI GCATC 2 cut(s) 312, 358
SfoI GGCGCC 1 cut(s) 670
SmlI CTYRAG 3 cut(s) 10, 245, 343
SmoI CTYRAG 3 cut(s) 10, 245, 343
SphI GCATGC 1 cut(s) 394
Sse9I AATT 3 cut(s) 378, 543, 629
SsiI CCGC 2 cut(s) 143, 331
SspDI GGCGCC 1 cut(s) 668
SspI AATATT 1 cut(s) 707
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 464, 720
TaqI TCGA 1 cut(s) 486
TasI AATT 3 cut(s) 378, 543, 629
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 4 cut(s) 6, 122, 517, 569
Tru1I TTAA 4 cut(s) 344, 446, 546, 642
Tru9I TTAA 4 cut(s) 344, 446, 546, 642
TseFI GTSAC 1 cut(s) 621
Tsp45I GTSAC 1 cut(s) 621
TspDTI ATGAA 2 cut(s) 296, 605
Vha464I CTTAAG 1 cut(s) 343
XbaI TCTAGA 1 cut(s) 520
XceI RCATGY 1 cut(s) 394
XspI CTAG 1 cut(s) 521
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.