pycom05g25740

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000586
Physical Location & Seq
Reverse (-)
27085876 .. 27087406
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25740.3

Sequence Viewer

Length: 723 bp
ATGATCTCCTCCGATATATCGCTCTTAGGACCCTCGGATGTGGCACACTATGCTGATCCTGACCTGCCGTATAGCCTTGATAGGATGTGCCGCCATGCCGTAAATCTTAGTGCTGGCAATTTGGTCGATATCAATATTGAGTCATTTGGCGAATTTGAGCTCCTTCAGTATATCACAAATAGTTCGAGTAAAATCAGAAGGCTCCGAATTGTGTGTTGCTATGGCATTTGCGATGAGGGATTGTCTGAAGTTGTTTCAAAATTTCCAATGTTGGAGGACATTGACTTAACATTATGCAAAAATATTACGCATAGGTCTCTGAAACTGATTGGCCGCTCTTACGACTGGTTCAAAGATGATAGTGATGATGAATTCTCTTTGAATAGAGATGAGGATGCAATTGCTGCAGCTCAAACGATGCCCGGTGTGCGCCACCTCCAGCTGTTTGGGAATCAGATAACTAATGAGCGCTTGAAGAAGATTCTAGATGGTTGTCCTCATCTCGAGTCACTTGATCTGCGGCATTGTTTCAATCTTGATCTCGGGGGAGATTTGGGAAGAATATGCGCTGAACGAATTAAAAAGCTGCTGCTTCCTAATGATTCCATTGCTGGCTATGAATTTAGCCCTTCAATTGATTGGCGTAATCAACAATTAAGTCGTCGTGAAAGCCCTGTACGACACTGGCGAGATCCGTACGGAAATTATGATAGTAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

241

Amino Acids

27.33

Weight (kDa)

5.08

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 72
AccBSI CCGCTC 1 cut(s) 336
AciI CCGC 3 cut(s) 91, 334, 520
AclWI GGATC 2 cut(s) 50, 686
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 4 cut(s) 152, 260, 371, 620
AcuI CTGAAG 2 cut(s) 149, 267
AfaI GTAC 2 cut(s) 678, 698
AfeI AGCGCT 1 cut(s) 470
AgsI TTSAA 6 cut(s) 258, 352, 382, 475, 532, 633
AluBI AGCT 4 cut(s) 160, 410, 442, 586
AluI AGCT 4 cut(s) 160, 410, 442, 586
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 2 cut(s) 50, 686
Ama87I CYCGRG 2 cut(s) 503, 542
Aor51HI AGCGCT 1 cut(s) 470
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 4 cut(s) 404, 407, 586, 589
ApoI RAATTY 4 cut(s) 152, 260, 371, 620
AspLEI GCGC 3 cut(s) 432, 471, 569
AspS9I GGNCC 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 423
AvaI CYCGRG 2 cut(s) 503, 542
AvaII GGWCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 391, 419, 573, 576
BccI CCATC 1 cut(s) 482
BceAI ACGGC 2 cut(s) 52, 83
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 7 cut(s) 91, 334, 405, 408, 521, 587, 590
BlsI GCNGC 7 cut(s) 92, 335, 406, 409, 522, 588, 591
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 29
BmeT110I CYCGRG 2 cut(s) 503, 542
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 2 cut(s) 31, 203
BmrFI CCNGG 1 cut(s) 423
BmsI GCATC 2 cut(s) 385, 408
BpmI CTGGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 350, 689
Bse3DI GCAATG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 43, 90, 400
BseMI GCAATG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 350, 689
BseXI GCAGC 4 cut(s) 391, 419, 573, 576
BshFI GGCC 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 2 cut(s) 503, 542
BsiSI CCGG 1 cut(s) 423
BsiWI CGTACG 1 cut(s) 696
BsmAI GTCTC 1 cut(s) 321
BsnI GGCC 1 cut(s) 333
Bso31I GGTCTC 1 cut(s) 321
BsoBI CYCGRG 2 cut(s) 503, 542
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 5 cut(s) 3, 55, 514, 538, 691
BspACI CCGC 3 cut(s) 91, 334, 520
BspANI GGCC 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 31, 203
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 2 cut(s) 50, 686
BspTNI GGTCTC 1 cut(s) 321
BsrBI CCGCTC 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 606
BsrI ACTGG 2 cut(s) 350, 689
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 5 cut(s) 3, 55, 514, 538, 691
BstAPI GCANNNNNTGC 2 cut(s) 50, 404
BstC8I GCNNGC 2 cut(s) 115, 613
BstDEI CTNAG 2 cut(s) 25, 107
BstF5I GGATG 3 cut(s) 43, 90, 400
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 3 cut(s) 432, 471, 569
BstKTI GATC 5 cut(s) 6, 58, 517, 541, 694
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 5 cut(s) 3, 55, 514, 538, 691
BstMWI GCNNNNNNNGC 3 cut(s) 50, 404, 427
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 4 cut(s) 391, 419, 573, 576
BstX2I RGATCY 1 cut(s) 691
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 1 cut(s) 691
BsuRI GGCC 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 43, 90, 400
BtsIMutI CAGTG 1 cut(s) 682
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 115, 613
CfoI GCGC 3 cut(s) 432, 471, 569
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 677, 697
CviAII CATG 1 cut(s) 95
CviQI GTAC 2 cut(s) 677, 697
DdeI CTNAG 2 cut(s) 25, 107
DpnI GATC 5 cut(s) 5, 57, 516, 540, 693
DpnII GATC 5 cut(s) 3, 55, 514, 538, 691
EaeI YGGCCR 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 29
Eco47III AGCGCT 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 149, 267
Eco88I CYCGRG 2 cut(s) 503, 542
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 371
EcoRV GATATC 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 1 cut(s) 98
FatI CATG 1 cut(s) 94
Fnu4HI GCNGC 7 cut(s) 91, 334, 405, 408, 521, 587, 590
FokI GGATG 3 cut(s) 50, 97, 407
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 7 cut(s) 91, 334, 405, 408, 521, 587, 590
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 431, 470, 568
GluI GCNGC 7 cut(s) 91, 334, 405, 408, 521, 587, 590
GsuI CTGGAG 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 423
HhaI GCGC 3 cut(s) 432, 471, 569
Hin1II CATG 1 cut(s) 98
Hin6I GCGC 3 cut(s) 430, 469, 567
HinP1I GCGC 3 cut(s) 430, 469, 567
HinfI GANTC 5 cut(s) 140, 451, 481, 506, 602
HpaII CCGG 1 cut(s) 423
Hpy188I TCNGA 7 cut(s) 13, 37, 197, 206, 247, 321, 456
Hpy188III TCNNGA 5 cut(s) 59, 485, 503, 536, 665
Hpy99I CGWCG 1 cut(s) 666
HpyAV CCTTC 3 cut(s) 173, 192, 639
HpyCH4V TGCA 3 cut(s) 297, 398, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 404, 427
HpyF3I CTNAG 2 cut(s) 25, 107
Hsp92II CATG 1 cut(s) 98
HspAI GCGC 3 cut(s) 430, 469, 567
Kzo9I GATC 5 cut(s) 3, 55, 514, 538, 691
LmnI GCTCC 2 cut(s) 165, 207
LpnPI CCDG 9 cut(s) 72, 77, 99, 331, 436, 452, 597, 670, 687
Lsp1109I GCAGC 4 cut(s) 391, 419, 573, 576
LweI GCATC 2 cut(s) 385, 408
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 507
MalI GATC 5 cut(s) 5, 57, 516, 540, 693
MbiI CCGCTC 1 cut(s) 336
MboI GATC 5 cut(s) 3, 55, 514, 538, 691
MboII GAAGA 3 cut(s) 487, 490, 570
MfeI CAATTG 2 cut(s) 399, 633
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 1 cut(s) 162
MlyI GAGTC 2 cut(s) 149, 515
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 7 cut(s) 19, 43, 229, 268, 385, 446, 507
MseI TTAA 3 cut(s) 287, 579, 656
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 1 cut(s) 423
MunI CAATTG 2 cut(s) 399, 633
MwoI GCNNNNNNNGC 3 cut(s) 50, 404, 427
NciI CCSGG 1 cut(s) 423
NdeII GATC 5 cut(s) 3, 55, 514, 538, 691
NlaIII CATG 1 cut(s) 98
NlaIV GGNNCC 2 cut(s) 31, 203
NmuCI GTSAC 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PfeI GAWTC 3 cut(s) 451, 481, 602
Pfl23II CGTACG 1 cut(s) 696
PkrI GCNGC 7 cut(s) 92, 335, 406, 409, 522, 588, 591
PleI GAGTC 2 cut(s) 148, 514
PpsI GAGTC 2 cut(s) 148, 514
PpuMI RGGWCCY 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 29
PspLI CGTACG 1 cut(s) 696
PspN4I GGNNCC 2 cut(s) 31, 203
PspPI GGNCC 1 cut(s) 29
PspPPI RGGWCCY 1 cut(s) 29
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 442
RsaI GTAC 2 cut(s) 678, 698
RsaNI GTAC 2 cut(s) 677, 697
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 3 cut(s) 287, 579, 656
SatI GCNGC 7 cut(s) 91, 334, 405, 408, 521, 587, 590
Sau3AI GATC 5 cut(s) 3, 55, 514, 538, 691
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 2 cut(s) 149, 515
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 162
SetI ASST 7 cut(s) 66, 162, 317, 412, 438, 444, 588
SfaNI GCATC 2 cut(s) 385, 408
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 503
SinI GGWCC 1 cut(s) 29
SlaI CTCGAG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SsiI CCGC 3 cut(s) 91, 334, 520
SspI AATATT 2 cut(s) 136, 304
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 421
TaqI TCGA 3 cut(s) 126, 185, 504
TauI GCSGC 3 cut(s) 93, 336, 523
TfiI GAWTC 3 cut(s) 451, 481, 602
Tru1I TTAA 3 cut(s) 287, 579, 656
Tru9I TTAA 3 cut(s) 287, 579, 656
TscAI CASTG 1 cut(s) 689
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 4 cut(s) 404, 407, 586, 589
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 2 cut(s) 384, 633
TspGWI ACGGA 2 cut(s) 684, 714
TspRI CASTG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 4 cut(s) 152, 260, 371, 620
XbaI TCTAGA 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 503
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.