Rorug05G0025800

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000586
Physical Location & Seq
Forward (+)
1652965 .. 1653906
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0025800.1

Sequence Viewer

Length: 564 bp
ATGAGTACTATCAGGAACATTGTCCGCAACCTCATGCGCCATAAGGGTCTTGGTAAGTTTTCGATTTTTGTTTCAGGGTTCGTGAGAAAGATTATCAGCAATTTTCTGGGGCTGATCCGTTTGTTCCGAAGGAAAAAAGAAACGATCGCGGTATGTGCTAGTTCGATTGATCAGGATCTGAAGCTAGGGCTCCTTAGTGAGGCTGAACACAAGAAAGAACATTATGAAGCATCTAGCTCCTTTACTCCTATGGAACTGCAAAGAGGTACCAAGGCAGAAGAACCCGAAGCTGCTGGAAACCCTTTGATTCAACCTGTAGTCAAAAGAGAACACATTTTCTGTCGGTACTGTTTTAGCGTTGGAGATCATAAAACTTCAGTTTGCCCGAAGAAACATTACATATGTACAGACTGTGGTACCCTATTTCCCTGCGAGAAGAAGGACGTCCATGGAGAAGAACATAAAGTATTAAATTATTGCTATGGTTGTAAAAGATGGGGCCACTTGTTTGAAATGTGCCCTGACCTTGATAAATTGGATGTAAACTTTGACCCTCTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

187

Amino Acids

21.45

Weight (kDa)

8.65

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 447
Acc65I GGTACC 2 cut(s) 266, 416
AccB1I GGYRCC 2 cut(s) 266, 416
AccII CGCG 1 cut(s) 149
AciI CCGC 2 cut(s) 25, 149
AclWI GGATC 2 cut(s) 109, 183
AcuI CTGAAG 2 cut(s) 200, 360
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 5 cut(s) 7, 268, 347, 406, 418
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 2 cut(s) 311, 512
AluBI AGCT 3 cut(s) 184, 237, 290
AluI AGCT 3 cut(s) 184, 237, 290
AlwI GGATC 2 cut(s) 109, 183
AlwNI CAGNNNCTG 1 cut(s) 178
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 1 cut(s) 290
Asp718I GGTACC 2 cut(s) 266, 416
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 499
BaeGI GKGCMC 1 cut(s) 521
BaeI ACNNNNGTAYC 2 cut(s) 400, 433
BanI GGYRCC 2 cut(s) 266, 416
BanII GRGCYC 1 cut(s) 192
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BbvI GCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 489
BclI TGATCA 1 cut(s) 169
BfaI CTAG 4 cut(s) 159, 185, 234, 557
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
BmcAI AGTACT 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 4 cut(s) 191, 268, 418, 500
BmsI GCATC 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 270, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 270, 448
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 501
BshNI GGYRCC 2 cut(s) 266, 416
BsiEI CGRYCG 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 199
BsnI GGCC 1 cut(s) 501
Bsp1286I GDGCHC 2 cut(s) 192, 521
Bsp1407I TGTACA 1 cut(s) 404
Bsp143I GATC 5 cut(s) 114, 144, 169, 175, 364
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 2 cut(s) 25, 149
BspANI GGCC 1 cut(s) 501
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 4 cut(s) 191, 268, 418, 500
BspPI GGATC 2 cut(s) 109, 183
BspT107I GGYRCC 2 cut(s) 266, 416
BsrGI TGTACA 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 270, 448
BssMI GATC 5 cut(s) 114, 144, 169, 175, 364
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 2 cut(s) 270, 448
Bst4CI ACNGT 2 cut(s) 350, 413
BstACI GRCGYC 1 cut(s) 444
BstAUI TGTACA 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 197
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 5 cut(s) 117, 147, 172, 178, 367
BstMBI GATC 5 cut(s) 114, 144, 169, 175, 364
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 1 cut(s) 544
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 5 cut(s) 6, 267, 346, 405, 417
CviAII CATG 2 cut(s) 34, 449
CviJI RGCY 7 cut(s) 112, 184, 190, 203, 237, 290, 501
CviKI_1 RGCY 7 cut(s) 112, 184, 190, 203, 237, 290, 501
CviQI GTAC 5 cut(s) 6, 267, 346, 405, 417
DdeI CTNAG 1 cut(s) 194
DpnI GATC 5 cut(s) 116, 146, 171, 177, 366
DpnII GATC 5 cut(s) 114, 144, 169, 175, 364
Eco130I CCWWGG 2 cut(s) 270, 448
Eco24I GRGCYC 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 200, 360
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoT14I CCWWGG 2 cut(s) 270, 448
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 270, 448
FaeI CATG 2 cut(s) 37, 452
FatI CATG 2 cut(s) 33, 448
FauNDI CATATG 1 cut(s) 401
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 4 cut(s) 159, 185, 234, 557
GlaI GCGC 1 cut(s) 38
GluI GCNGC 1 cut(s) 291
HaeIII GGCC 1 cut(s) 501
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 2 cut(s) 37, 452
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 2 cut(s) 128, 180
Hpy188III TCNNGA 4 cut(s) 13, 82, 173, 557
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 2 cut(s) 123, 433
HpyCH4III ACNGT 2 cut(s) 350, 413
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 2 cut(s) 37, 452
HspAI GCGC 1 cut(s) 37
KpnI GGTACC 2 cut(s) 270, 420
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 114, 144, 169, 175, 364
LmnI GCTCC 2 cut(s) 195, 242
LpnPI CCDG 7 cut(s) 60, 92, 158, 279, 327, 442, 534
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 239
MaeI CTAG 4 cut(s) 159, 185, 234, 557
MaeII ACGT 1 cut(s) 444
MalI GATC 5 cut(s) 116, 146, 171, 177, 366
MboI GATC 5 cut(s) 114, 144, 169, 175, 364
MboII GAAGA 4 cut(s) 290, 400, 448, 467
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 192, 521
MluCI AATT 3 cut(s) 100, 472, 533
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 4 cut(s) 41, 193, 257, 564
MseI TTAA 1 cut(s) 470
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 401
NdeII GATC 5 cut(s) 114, 144, 169, 175, 364
NlaIII CATG 2 cut(s) 37, 452
NlaIV GGNNCC 4 cut(s) 191, 268, 418, 500
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 1 cut(s) 292
Ple19I CGATCG 1 cut(s) 147
PspN4I GGNNCC 4 cut(s) 191, 268, 418, 500
PspPI GGNCC 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 175
PvuI CGATCG 1 cut(s) 147
RsaI GTAC 5 cut(s) 7, 268, 347, 406, 418
RsaNI GTAC 5 cut(s) 6, 267, 346, 405, 417
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 5 cut(s) 114, 144, 169, 175, 364
Sau96I GGNCC 1 cut(s) 499
ScaI AGTACT 1 cut(s) 7
SduI GDGCHC 2 cut(s) 192, 521
SetI ASST 8 cut(s) 33, 186, 239, 268, 292, 316, 447, 528
SfaNI GCATC 1 cut(s) 239
SfcI CTRYAG 1 cut(s) 315
Sse9I AATT 3 cut(s) 100, 472, 533
SsiI CCGC 2 cut(s) 25, 149
SspMI CTAG 4 cut(s) 159, 185, 234, 557
StyI CCWWGG 2 cut(s) 270, 448
TaaI ACNGT 2 cut(s) 350, 413
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 62, 164
TasI AATT 3 cut(s) 100, 472, 533
TatI WGTACW 2 cut(s) 5, 404
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TseI GCWGC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 240
TspGWI ACGGA 1 cut(s) 107
XagI CCTNNNNNAGG 1 cut(s) 197
XbaI TCTAGA 1 cut(s) 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XspI CTAG 4 cut(s) 159, 185, 234, 557
ZraI GACGTC 1 cut(s) 445
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.