Rorug05G0025500

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000586
Physical Location & Seq
Reverse (-)
1626218 .. 1628416
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0025500.1

Sequence Viewer

Length: 1353 bp
ATGAAAGATTGTCGAGTGAATACTGTGTCTATGGTTTCGGCAGTGAATGATGAACTAATGAAAGCTAGATTGAGCATTCGAGAGCTTGAAGCTAAGCGGAGGTCTTTCAGGAAGAGAGTCAAACACTTTTTGAGTAAGCTTGAAGAGGAAAGAGTATTGCAGAAGCGCAGAGAGTGCCAGAGAGATCGTGCGGTTATTAAGGAGTTGATGAAAGAACTAAGCAGGGAAAGGAGGAGTCGTCAATGGATGGAAAATCTTAGCACCAATCTGGTCAGTCGGTTAGCCAATGCAAAGTTGTCTGCAGACCAGTTCATGAAAACGTACAAGGAAGAGAAACAAAACAGAAGATTCATGGAACAAGTTTGTAGCGAACTAGCTGAACACCTTGGAGAGAACAAGGCTGAATTGAAGGCATTGAAAAGAGAATCCATGAAAATTATTGATGAATTGGAAGAAGAGAGAAAGATGTTGCAGATGACTGAGGCTTGGCGTGAAGAACGAGTGCAGATGAAGCTGATTGATGCAAAACTGGCTCTTGAAGATAAATATCATCAGGTGAACAAGCTGATAACAGATGTTGAAACTTTCTTAAGGTCAAGGTTTGCTACCCTGGATATGCCGGAATTAAGAAAGGCTGAGTTGATCCTACAGGAAGTTAAGTCGTTGAATATTCAAGATATCGAGGAATTTACATATATTGCTCTGAAATCAGATGATATATTCTCTACTTTTGAAGAGTTCCGACTATATGAAGGTAAAGGGAGAGAGATTAAACCTTGCATTTGTCACAGTGCTTCTAGCCATGATTCGAAATTCCATCCGGTGAGTCCTGACAATAATCGGTTCAACAAAACCCCTTTGCCAAGTTACTCGAATTGTCTCGACGGTTATAGTAGTGGTGTGCAAGAAAGTGCTAGAGGTTGGGCAAATGGGAGTCATGGTCAATCCTCTCCAGATACTGAAATCATTGAAGTTTGCCCAGTATCGGAGCAGCAATCAGAGCAGAAGCTGTCTTCTATGTCCAAGCTTTGGAGATCATGCCCAACTAATGGGGAAGTTTTTAAGATAATTTTGGATGAAGACAAGAGCAGGCTCTCAAATGGGGTTACATCATGTTTGCAAAGAAATGCTGATAGTAGACTGACTGAACATGAGACCAGACACCGGGATTCAAATGAACATTTGTTCCCACCGGACATAGCGAATCCACACATCACTCGAGGGATGAAAGGACGCATTGAATGGCCCCGAGCCATCCAAAAGAATGGTTCAAATGCCAAGCTTTTGGAAGCAAAAATAGAAAGCCAGAGATCTCAACTGCGCCATGTTTTAGGGTTTCGTTATGATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

450

Amino Acids

52.61

Weight (kDa)

8.79

Isoelectric Point (pI)

62.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1029, 1049
AccI GTMKAC 1 cut(s) 1138
AciI CCGC 2 cut(s) 97, 191
AclWI GGATC 1 cut(s) 637
AcsI RAATTY 2 cut(s) 686, 812
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 4 cut(s) 985, 1029, 1049, 1164
AflII CTTAAG 1 cut(s) 589
AjnI CCWGG 1 cut(s) 609
AloI GAACNNNNNNTCC 2 cut(s) 1170, 1202
Alw26I GTCTC 2 cut(s) 884, 1148
AlwI GGATC 1 cut(s) 637
AlwNI CAGNNNCTG 2 cut(s) 959, 1009
Ama87I CYCGRG 2 cut(s) 1218, 1248
AoxI GGCC 1 cut(s) 1244
ApeKI GCWGC 1 cut(s) 991
ApoI RAATTY 2 cut(s) 686, 812
AspLEI GCGC 2 cut(s) 168, 1323
AspS9I GGNCC 1 cut(s) 1245
AsuC2I CCSGG 1 cut(s) 1166
AsuHPI GGTGA 2 cut(s) 568, 835
AsuII TTCGAA 1 cut(s) 809
AvaI CYCGRG 2 cut(s) 1218, 1248
BbsI GAAGAC 2 cut(s) 1005, 1086
BbvI GCAGC 1 cut(s) 1003
BccI CCATC 3 cut(s) 241, 825, 1262
BciT130I CCWGG 1 cut(s) 611
BcnI CCSGG 1 cut(s) 1166
BcoDI GTCTC 2 cut(s) 884, 1148
BfaI CTAG 4 cut(s) 66, 374, 798, 915
BfmI CTRYAG 2 cut(s) 300, 647
BfrI CTTAAG 1 cut(s) 589
BglII AGATCT 1 cut(s) 1310
BisI GCNGC 1 cut(s) 992
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 993
Bme1390I CCNGG 2 cut(s) 611, 1166
BmeT110I CYCGRG 2 cut(s) 1218, 1248
BmgT120I GGNCC 1 cut(s) 1245
BmiI GGNNCC 1 cut(s) 1247
BmrFI CCNGG 2 cut(s) 611, 1166
BmrI ACTGGG 1 cut(s) 974
BmsI GCATC 1 cut(s) 511
BmuI ACTGGG 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 1005, 1086
BpmI CTGGAG 1 cut(s) 936
Bpu1102I GCTNAGC 1 cut(s) 93
Bpu14I TTCGAA 1 cut(s) 809
BpuMI CCSGG 1 cut(s) 1166
BsaBI GATNNNNATC 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 2 cut(s) 385, 609
BsaWI WCCGGW 2 cut(s) 820, 1192
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 925, 955
Bsc4I CCNNNNNNNGG 4 cut(s) 985, 1029, 1049, 1164
Bse1I ACTGG 3 cut(s) 307, 534, 980
Bse8I GATNNNNATC 1 cut(s) 546
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 385, 609
BseGI GGATG 5 cut(s) 252, 817, 1081, 1230, 1254
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 4 cut(s) 985, 1029, 1049, 1164
BseMII CTCAG 2 cut(s) 471, 627
BseNI ACTGG 3 cut(s) 307, 534, 980
BseRI GAGGAG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 1003
BsgI GTGCAG 1 cut(s) 524
BshFI GGCC 1 cut(s) 1246
BsiHKCI CYCGRG 2 cut(s) 1218, 1248
BsiSI CCGG 4 cut(s) 620, 821, 1165, 1193
BslI CCNNNNNNNGG 4 cut(s) 985, 1029, 1049, 1164
BsmAI GTCTC 2 cut(s) 884, 1148
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 1 cut(s) 1246
Bso31I GGTCTC 1 cut(s) 1148
BsoBI CYCGRG 2 cut(s) 1218, 1248
Bsp119I TTCGAA 1 cut(s) 809
Bsp143I GATC 4 cut(s) 184, 642, 1034, 1310
Bsp1720I GCTNAGC 1 cut(s) 93
BspACI CCGC 2 cut(s) 97, 191
BspANI GGCC 1 cut(s) 1246
BspCNI CTCAG 2 cut(s) 472, 628
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 1247
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 637
BspT104I TTCGAA 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 1148
BsrI ACTGG 3 cut(s) 307, 534, 980
BssECI CCNNGG 2 cut(s) 385, 609
BssMI GATC 4 cut(s) 184, 642, 1034, 1310
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 3 cut(s) 25, 791, 887
Bst6I CTCTTC 5 cut(s) 107, 138, 324, 450, 729
BstAFI CTTAAG 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 1 cut(s) 1091
BstDEI CTNAG 5 cut(s) 93, 218, 257, 480, 636
BstF5I GGATG 5 cut(s) 252, 817, 1081, 1230, 1254
BstHHI GCGC 2 cut(s) 168, 1323
BstKTI GATC 4 cut(s) 187, 645, 1037, 1313
BstMAI GTCTC 2 cut(s) 884, 1148
BstMBI GATC 4 cut(s) 184, 642, 1034, 1310
BstMWI GCNNNNNNNGC 4 cut(s) 174, 511, 530, 1000
BstNI CCWGG 1 cut(s) 611
BstSCI CCNGG 2 cut(s) 609, 1164
BstSFI CTRYAG 2 cut(s) 300, 647
BstV1I GCAGC 1 cut(s) 1003
BstV2I GAAGAC 2 cut(s) 1005, 1086
BstX2I RGATCY 1 cut(s) 1310
BstXI CCANNNNNNTGG 2 cut(s) 1265, 1285
BstYI RGATCY 1 cut(s) 1310
BsuRI GGCC 1 cut(s) 1246
BtsCI GGATG 5 cut(s) 252, 817, 1081, 1230, 1254
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 796
Cac8I GCNNGC 1 cut(s) 1091
CaiI CAGNNNCTG 2 cut(s) 959, 1009
CciI TCATGA 1 cut(s) 312
CfoI GCGC 2 cut(s) 168, 1323
Cfr13I GGNCC 1 cut(s) 1245
CseI GACGC 1 cut(s) 1242
Csp6I GTAC 1 cut(s) 322
CviAII CATG 9 cut(s) 313, 352, 430, 803, 938, 1038, 1113, 1151, 1325
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 5 cut(s) 93, 218, 257, 480, 636
DpnI GATC 4 cut(s) 186, 644, 1036, 1312
DpnII GATC 4 cut(s) 184, 642, 1034, 1310
Eam1104I CTCTTC 5 cut(s) 107, 138, 324, 450, 729
EarI CTCTTC 5 cut(s) 107, 138, 324, 450, 729
Eco130I CCWWGG 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 1148
Eco32I GATATC 1 cut(s) 679
Eco88I CYCGRG 2 cut(s) 1218, 1248
EcoRII CCWGG 1 cut(s) 609
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 9 cut(s) 316, 355, 433, 806, 941, 1041, 1116, 1154, 1328
FatI CATG 9 cut(s) 312, 351, 429, 802, 937, 1037, 1112, 1150, 1324
FblI GTMKAC 1 cut(s) 1138
Fnu4HI GCNGC 1 cut(s) 992
FokI GGATG 5 cut(s) 259, 804, 1088, 1237, 1241
Fsp4HI GCNGC 1 cut(s) 992
FspBI CTAG 4 cut(s) 66, 374, 798, 915
GlaI GCGC 2 cut(s) 167, 1322
GluI GCNGC 1 cut(s) 992
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 1 cut(s) 1246
HapII CCGG 4 cut(s) 620, 821, 1165, 1193
HgaI GACGC 1 cut(s) 1242
HhaI GCGC 2 cut(s) 168, 1323
Hin1II CATG 9 cut(s) 316, 355, 433, 806, 941, 1041, 1116, 1154, 1328
Hin6I GCGC 2 cut(s) 166, 1321
HinP1I GCGC 2 cut(s) 166, 1321
HindIII AAGCTT 3 cut(s) 137, 1025, 1280
HinfI GANTC 9 cut(s) 117, 235, 348, 425, 806, 826, 934, 1169, 1204
HpaII CCGG 4 cut(s) 620, 821, 1165, 1193
HphI GGTGA 2 cut(s) 568, 835
Hpy166II GTNNAC 2 cut(s) 559, 1139
Hpy188I TCNGA 5 cut(s) 705, 712, 743, 988, 1000
Hpy188III TCNNGA 8 cut(s) 80, 109, 313, 536, 674, 830, 881, 953
Hpy8I GTNNAC 2 cut(s) 559, 1139
Hpy99I CGWCG 1 cut(s) 887
HpyAV CCTTC 2 cut(s) 403, 746
HpyCH4III ACNGT 3 cut(s) 25, 791, 887
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 9 cut(s) 160, 290, 302, 472, 505, 524, 780, 904, 1120
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 511, 530, 1000
HpyF3I CTNAG 5 cut(s) 93, 218, 257, 480, 636
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 9 cut(s) 316, 355, 433, 806, 941, 1041, 1116, 1154, 1328
HspAI GCGC 2 cut(s) 166, 1321
Kzo9I GATC 4 cut(s) 184, 642, 1034, 1310
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 1 cut(s) 1003
LweI GCATC 1 cut(s) 511
MaeI CTAG 4 cut(s) 66, 374, 798, 915
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 3 cut(s) 785, 866, 1105
MalI GATC 4 cut(s) 186, 644, 1036, 1312
MboI GATC 4 cut(s) 184, 642, 1034, 1310
MflI RGATCY 1 cut(s) 1310
MluCI AATT 8 cut(s) 404, 435, 446, 623, 686, 812, 874, 1068
MlyI GAGTC 4 cut(s) 126, 244, 835, 943
MmeI TCCRAC 1 cut(s) 766
MnlI CCTC 8 cut(s) 93, 139, 225, 475, 676, 911, 958, 1214
MseI TTAA 6 cut(s) 198, 590, 626, 657, 771, 1062
MspCI CTTAAG 1 cut(s) 589
MspI CCGG 4 cut(s) 620, 821, 1165, 1193
MspR9I CCNGG 2 cut(s) 611, 1166
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 4 cut(s) 174, 511, 530, 1000
NciI CCSGG 1 cut(s) 1166
NdeII GATC 4 cut(s) 184, 642, 1034, 1310
NlaIII CATG 9 cut(s) 316, 355, 433, 806, 941, 1041, 1116, 1154, 1328
NlaIV GGNNCC 1 cut(s) 1247
NmuCI GTSAC 1 cut(s) 785
NspV TTCGAA 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 1218
PagI TCATGA 1 cut(s) 312
PctI GAATGC 1 cut(s) 75
PfeI GAWTC 5 cut(s) 348, 425, 806, 1169, 1204
PflMI CCANNNNNTGG 2 cut(s) 1029, 1049
PkrI GCNGC 1 cut(s) 993
PleI GAGTC 4 cut(s) 125, 243, 834, 942
PpsI GAGTC 4 cut(s) 125, 243, 834, 942
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 1 cut(s) 1247
PspPI GGNCC 1 cut(s) 1245
PspXI VCTCGAGB 1 cut(s) 1218
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 2 cut(s) 959, 1009
PsuI RGATCY 1 cut(s) 1310
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SaqAI TTAA 6 cut(s) 198, 590, 626, 657, 771, 1062
SatI GCNGC 1 cut(s) 992
Sau3AI GATC 4 cut(s) 184, 642, 1034, 1310
Sau96I GGNCC 1 cut(s) 1245
SchI GAGTC 4 cut(s) 126, 244, 835, 943
ScrFI CCNGG 2 cut(s) 611, 1166
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 2 cut(s) 300, 647
Sfr274I CTCGAG 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 809
SlaI CTCGAG 1 cut(s) 1218
SmlI CTYRAG 2 cut(s) 589, 1218
SmoI CTYRAG 2 cut(s) 589, 1218
Sse9I AATT 8 cut(s) 404, 435, 446, 623, 686, 812, 874, 1068
SsiI CCGC 2 cut(s) 97, 191
SspI AATATT 1 cut(s) 670
SspMI CTAG 4 cut(s) 66, 374, 798, 915
StyD4I CCNGG 2 cut(s) 609, 1164
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 3 cut(s) 25, 791, 887
TaiI ACGT 1 cut(s) 323
TaqI TCGA 7 cut(s) 13, 79, 681, 809, 872, 882, 1219
TasI AATT 8 cut(s) 404, 435, 446, 623, 686, 812, 874, 1068
TfiI GAWTC 5 cut(s) 348, 425, 806, 1169, 1204
Tru1I TTAA 6 cut(s) 198, 590, 626, 657, 771, 1062
Tru9I TTAA 6 cut(s) 198, 590, 626, 657, 771, 1062
TscAI CASTG 2 cut(s) 48, 796
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 1 cut(s) 991
Tsp45I GTSAC 1 cut(s) 785
TspRI CASTG 2 cut(s) 48, 796
Van91I CCANNNNNTGG 2 cut(s) 1029, 1049
Vha464I CTTAAG 1 cut(s) 589
XapI RAATTY 2 cut(s) 686, 812
XhoI CTCGAG 1 cut(s) 1218
XmiI GTMKAC 1 cut(s) 1138
XspI CTAG 4 cut(s) 66, 374, 798, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.