FvH4_2g01110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000977
Physical Location & Seq
Forward (+)
1055076 .. 1059221
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01110.t1

Sequence Viewer

Length: 1770 bp
ATGTCCACCATATGCAGTTCTTCAATTGTTTACTTCTTGCCTACAAGAAAGTGGCAAGGACTTCCCAAGTATCCTAGCCCGAGAAAAACTAACAATCGAGAGGTTTTGGTTCAAAAGGTCGACATATTACAGCGACTGATGTTCTGGCTGGAGCGGCTTGTGGTCGTGGTGTGCATCTTTTTGATATCCAGATTTGCTCGTTTCTCTACCGTGTTCTTCTGTTTTTCTGATTTCATCTACTTGATCTGCTTCGCTCTGAGGTTAGAGGTTGATCCTTTGCCAGCGGAAGCTGTAAAGGGCATAGAAGCAGAGAAAAAGGAAGCCAAGACGGAGGATGAACCACAAAAGGAGAAATTTGAAGCTCACCAAGGAGAAAAGAGCGATTGGGAAAAGGAATTGGTGGCTAAATATGGGTTTATGGATCATATATCAGAGTTCACATATACCAAAATTGACGATGAGTCGCCTATACAAATGTCTGAAACCCATCACCAAGATGTAAGGAACATAAGGGAAGAGGTATTGGTGCCTGAATACGGGTATATGGAGCATATATCAGAAGTGACATGTGCCAGACATCAGCCAAAGGAAGAAAGATACAATTGGGAAGATAAATTGGTGGGTAAACATGGGATGATGGAGCATGGATTAGAATTCACCTTCACCAGACCTAAGGAGACTAATAAGCAATCTGAAATTGAAGAAGAAGCACCAGATGAAGAGAATTACAAATCACCGAGAGAAGAGGATTTGGAAGTTGTGGAAGCACCGAAAGAAGAAGACAGAAAATTTTTCGATGAGGGTTCAGGATGGCAATGGTTTGATGATATGTCGTCAGAAGGACTTGATCCTTATGGTTATTTTGATAGATATGGAAAAGTTGATGATAACTGCGGAAACCCATTGGTGATGGCTGATAATGATCTGATGGAATATTTTATGAAGAGCACAACAGCTGAAAATGAAAGCAATAAGGAAGCACTTCTTGTGGTCACTGGAGAAGTTGAGAGACTGAGAGCTGAAAATGATGACTTGAAGAGAGAAAAGGAAGCATTGGAGAAGGAAAAGGAAGCATTGGAGGATCAATTAGATGCAGAGAGACTTGAACTTCAAGATACTCTCCTCGCGTGGAAAATGGACAGTCAGAAGCTTGATGCAATGCCTGTTGAAGCGAAGATGAAGTCTCAAGCTTTAGTTGAAGAGTTAGATTCTCACTCCAAGTTTGAGGAACAAATGCTGGAAACGCTGAAAACTTATAAAAAGGATTGCAGAAAATGGGAGCAAGAAAATAAAGACATTAGGGCAAAATTTGAAGAGTTAACTGCAGCTATTGGAAAAGATCAGCAGACTTTGCTAAATGGTCAAGGACATGACTGCAGGTACAAGAAAAGAAGACAGAAATGGAAAAGTGAGAAAATCGCACTCAAACAACAGCTTGTGGCAGCAAGTTCTTGGTACCAGTACTATTTTCAAGTGGCAATGACAGCAAGTTACTGGAACCAATACTATATGCAAAGCGAAAATGATAACGGAATGTGGATGCGTTACTATGAACACATGATCAACAGCCTTGTCGAAAGTAACACTGACCTTGCTAGAAGGCTTGAAGTAGAAACTAGTTTGGCTACACGTTATCACAATCGTGTTTTGCACTACGAAGAGAGATGCAGGAAGCTTGAGGAAGACAAGAACAATCTCACAGCAAAGCTTTGTGCTGCCAATGCCTCATCTGAACTATATCGAAGCATGTTACATGACTTAGCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

590

Amino Acids

69.57

Weight (kDa)

5.02

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1257
Acc36I ACCTGC 1 cut(s) 1368
Acc65I GGTACC 1 cut(s) 1455
AccB1I GGYRCC 2 cut(s) 526, 1455
AccBSI CCGCTC 1 cut(s) 154
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 1127
AciI CCGC 3 cut(s) 154, 284, 894
AclWI GGATC 4 cut(s) 266, 429, 842, 1089
AcsI RAATTY 4 cut(s) 353, 653, 788, 1307
AfaI GTAC 3 cut(s) 1382, 1457, 1463
AfiI CCNNNNNNNGG 1 cut(s) 536
AflIII ACRYGT 2 cut(s) 566, 1628
AhdI GACNNNNNGTC 1 cut(s) 460
AhlI ACTAGT 1 cut(s) 1616
AleI CACNNNNGTG 1 cut(s) 1641
Alw21I GWGCWC 1 cut(s) 950
Alw26I GTCTC 4 cut(s) 671, 1003, 1093, 1188
AlwI GGATC 4 cut(s) 266, 429, 842, 1089
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 79
ApeKI GCWGC 3 cut(s) 1325, 1442, 1715
ApoI RAATTY 4 cut(s) 353, 653, 788, 1307
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
Asp700I GAANNNNTTC 1 cut(s) 981
Asp718I GGTACC 1 cut(s) 1455
AsuHPI GGTGA 6 cut(s) 356, 482, 649, 655, 726, 919
AvaI CYCGRG 1 cut(s) 79
AxyI CCTNAGG 1 cut(s) 672
BanI GGYRCC 2 cut(s) 526, 1455
BbsI GAAGAC 3 cut(s) 786, 1399, 1689
Bbv12I GWGCWC 1 cut(s) 950
BbvI GCAGC 3 cut(s) 1337, 1454, 1702
BccI CCATC 5 cut(s) 495, 631, 804, 904, 922
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 1560
BcoDI GTCTC 4 cut(s) 671, 1003, 1093, 1188
BcuI ACTAGT 1 cut(s) 1616
BfaI CTAG 3 cut(s) 75, 1596, 1617
BfmI CTRYAG 2 cut(s) 1323, 1375
BfuAI ACCTGC 1 cut(s) 1368
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 4 cut(s) 155, 1326, 1443, 1716
BlsI GCNGC 4 cut(s) 156, 1327, 1444, 1717
BmcAI AGTACT 1 cut(s) 1463
BmeRI GACNNNNNGTC 1 cut(s) 460
BmeT110I CYCGRG 1 cut(s) 79
BmiI GGNNCC 3 cut(s) 528, 1457, 1499
BmsI GCATC 5 cut(s) 183, 1081, 1144, 1530, 1655
BpiI GAAGAC 3 cut(s) 786, 1399, 1689
BpmI CTGGAG 2 cut(s) 170, 1017
BpuEI CTTGAG 2 cut(s) 1170, 1697
BsaBI GATNNNNATC 1 cut(s) 921
BsaJI CCNNGG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse1I ACTGG 3 cut(s) 1000, 1459, 1499
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 3 cut(s) 821, 1164, 1485
Bse8I GATNNNNATC 1 cut(s) 921
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 4 cut(s) 340, 639, 815, 1545
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMI GCAATG 3 cut(s) 821, 1164, 1485
BseMII CTCAG 2 cut(s) 248, 1004
BseNI ACTGG 3 cut(s) 1000, 1459, 1499
BseRI GAGGAG 1 cut(s) 1112
BseXI GCAGC 3 cut(s) 1337, 1454, 1702
Bsh1236I CGCG 1 cut(s) 1127
BshNI GGYRCC 2 cut(s) 526, 1455
BsiHKAI GWGCWC 1 cut(s) 950
BsiHKCI CYCGRG 1 cut(s) 79
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 4 cut(s) 671, 1003, 1093, 1188
BsoBI CYCGRG 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 950
Bsp143I GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
BspACI CCGC 3 cut(s) 154, 284, 894
BspCNI CTCAG 2 cut(s) 249, 1005
BspFNI CGCG 1 cut(s) 1127
BspLI GGNNCC 3 cut(s) 528, 1457, 1499
BspMAI CTGCAG 2 cut(s) 1327, 1379
BspMI ACCTGC 1 cut(s) 1368
BspPI GGATC 4 cut(s) 266, 429, 842, 1089
BspQI GCTCTTC 1 cut(s) 938
BspT107I GGYRCC 2 cut(s) 526, 1455
BsrBI CCGCTC 1 cut(s) 154
BsrDI GCAATG 3 cut(s) 821, 1164, 1485
BsrI ACTGG 3 cut(s) 1000, 1459, 1499
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
BssT1I CCWWGG 1 cut(s) 367
Bst4CI ACNGT 2 cut(s) 211, 1142
Bst6I CTCTTC 8 cut(s) 510, 714, 738, 938, 1031, 1194, 1308, 1653
BstAPI GCANNNNNTGC 1 cut(s) 1351
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 5 cut(s) 257, 672, 1013, 1759, 1767
BstF5I GGATG 4 cut(s) 340, 639, 815, 1545
BstFNI CGCG 1 cut(s) 1127
BstKTI GATC 8 cut(s) 246, 274, 424, 850, 925, 1084, 1342, 1563
BstMAI GTCTC 4 cut(s) 671, 1003, 1093, 1188
BstMBI GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
BstMWI GCNNNNNNNGC 5 cut(s) 154, 1243, 1351, 1484, 1721
BstNSI RCATGY 2 cut(s) 570, 1750
BstSFI CTRYAG 2 cut(s) 1323, 1375
BstUI CGCG 1 cut(s) 1127
BstV1I GCAGC 3 cut(s) 1337, 1454, 1702
BstV2I GAAGAC 3 cut(s) 786, 1399, 1689
Bsu36I CCTNAGG 1 cut(s) 672
BsuI GTATCC 1 cut(s) 81
BtsCI GGATG 4 cut(s) 340, 639, 815, 1545
BtsIMutI CAGTG 2 cut(s) 993, 1584
BveI ACCTGC 1 cut(s) 1368
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 136
Csp6I GTAC 3 cut(s) 1381, 1456, 1462
CviAII CATG 7 cut(s) 567, 629, 644, 1370, 1558, 1747, 1754
CviQI GTAC 3 cut(s) 1381, 1456, 1462
DdeI CTNAG 5 cut(s) 257, 672, 1013, 1759, 1767
DpnI GATC 8 cut(s) 245, 273, 423, 849, 924, 1083, 1341, 1562
DpnII GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
DriI GACNNNNNGTC 1 cut(s) 460
Eam1104I CTCTTC 8 cut(s) 510, 714, 738, 938, 1031, 1194, 1308, 1653
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 8 cut(s) 510, 714, 738, 938, 1031, 1194, 1308, 1653
Eco130I CCWWGG 1 cut(s) 367
Eco32I GATATC 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 653
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 7 cut(s) 570, 632, 647, 1373, 1561, 1750, 1757
FalI AAGNNNNNCTT 2 cut(s) 969, 1001
FatI CATG 7 cut(s) 566, 628, 643, 1369, 1557, 1746, 1753
FauNDI CATATG 1 cut(s) 11
FbaI TGATCA 1 cut(s) 1560
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 4 cut(s) 155, 1326, 1443, 1716
FokI GGATG 4 cut(s) 347, 646, 822, 1552
Fsp4HI GCNGC 4 cut(s) 155, 1326, 1443, 1716
FspBI CTAG 3 cut(s) 75, 1596, 1617
GluI GCNGC 4 cut(s) 155, 1326, 1443, 1716
GsuI CTGGAG 2 cut(s) 170, 1017
Hin1II CATG 7 cut(s) 570, 632, 647, 1373, 1561, 1750, 1757
HincII GTYRAC 2 cut(s) 121, 1320
HindII GTYRAC 2 cut(s) 121, 1320
HindIII AAGCTT 4 cut(s) 1148, 1188, 1673, 1706
HinfI GANTC 2 cut(s) 461, 1208
HpaI GTTAAC 1 cut(s) 1320
HphI GGTGA 6 cut(s) 356, 482, 649, 655, 726, 919
Hpy166II GTNNAC 6 cut(s) 6, 31, 121, 438, 626, 1320
Hpy188III TCNNGA 4 cut(s) 98, 189, 807, 1112
Hpy8I GTNNAC 6 cut(s) 6, 31, 121, 438, 626, 1320
HpyAV CCTTC 4 cut(s) 670, 833, 1054, 1593
HpyCH4III ACNGT 2 cut(s) 211, 1142
HpyCH4IV ACGT 1 cut(s) 1630
HpyF10VI GCNNNNNNNGC 5 cut(s) 154, 1243, 1351, 1484, 1721
HpyF3I CTNAG 5 cut(s) 257, 672, 1013, 1759, 1767
HpySE526I ACGT 1 cut(s) 1630
Hsp92II CATG 7 cut(s) 570, 632, 647, 1373, 1561, 1750, 1757
KpnI GGTACC 1 cut(s) 1459
Ksp22I TGATCA 1 cut(s) 1560
KspAI GTTAAC 1 cut(s) 1320
Kzo9I GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
LguI GCTCTTC 1 cut(s) 938
LmnI GCTCC 4 cut(s) 151, 547, 640, 1279
Lsp1109I GCAGC 3 cut(s) 1337, 1454, 1702
LweI GCATC 5 cut(s) 183, 1081, 1144, 1530, 1655
MaeI CTAG 3 cut(s) 75, 1596, 1617
MaeII ACGT 1 cut(s) 1630
MaeIII GTNAC 6 cut(s) 562, 991, 1490, 1544, 1580, 1749
MalI GATC 8 cut(s) 245, 273, 423, 849, 924, 1083, 1341, 1562
MbiI CCGCTC 1 cut(s) 154
MboI GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
MfeI CAATTG 2 cut(s) 24, 601
MhlI GDGCHC 1 cut(s) 950
MlyI GAGTC 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 981
MseI TTAA 1 cut(s) 1319
MslI CAYNNNNRTG 2 cut(s) 495, 1641
MspA1I CMGCKG 2 cut(s) 284, 956
MunI CAATTG 2 cut(s) 24, 601
MvnI CGCG 1 cut(s) 1127
MwoI GCNNNNNNNGC 5 cut(s) 154, 1243, 1351, 1484, 1721
NdeI CATATG 1 cut(s) 11
NdeII GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
NlaIII CATG 7 cut(s) 570, 632, 647, 1373, 1561, 1750, 1757
NlaIV GGNNCC 3 cut(s) 528, 1457, 1499
NmuCI GTSAC 2 cut(s) 562, 991
NspI RCATGY 2 cut(s) 570, 1750
OliI CACNNNNGTG 1 cut(s) 1641
PciI ACATGT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 938
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 1 cut(s) 1208
PkrI GCNGC 4 cut(s) 156, 1327, 1444, 1717
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PscI ACATGT 1 cut(s) 566
PsiI TTATAA 1 cut(s) 1257
PspN4I GGNNCC 3 cut(s) 528, 1457, 1499
PstI CTGCAG 2 cut(s) 1327, 1379
PstNI CAGNNNCTG 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 3 cut(s) 1382, 1457, 1463
RsaNI GTAC 3 cut(s) 1381, 1456, 1462
RseI CAYNNNNRTG 2 cut(s) 495, 1641
SalI GTCGAC 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 938
SaqAI TTAA 1 cut(s) 1319
SatI GCNGC 4 cut(s) 155, 1326, 1443, 1716
Sau3AI GATC 8 cut(s) 243, 271, 421, 847, 922, 1081, 1339, 1560
ScaI AGTACT 1 cut(s) 1463
SchI GAGTC 1 cut(s) 470
SduI GDGCHC 1 cut(s) 950
SfaNI GCATC 5 cut(s) 183, 1081, 1144, 1530, 1655
SfcI CTRYAG 2 cut(s) 1323, 1375
SmiMI CAYNNNNRTG 2 cut(s) 495, 1641
SmlI CTYRAG 2 cut(s) 1185, 1676
SmoI CTYRAG 2 cut(s) 1185, 1676
SpeI ACTAGT 1 cut(s) 1616
SsiI CCGC 3 cut(s) 154, 284, 894
SspI AATATT 1 cut(s) 935
SspMI CTAG 3 cut(s) 75, 1596, 1617
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 211, 1142
TaiI ACGT 1 cut(s) 1633
TaqI TCGA 5 cut(s) 97, 120, 795, 1575, 1741
TatI WGTACW 1 cut(s) 1461
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 1208
Tru1I TTAA 1 cut(s) 1319
Tru9I TTAA 1 cut(s) 1319
TscAI CASTG 2 cut(s) 1000, 1591
TseFI GTSAC 2 cut(s) 562, 991
TseI GCWGC 3 cut(s) 1325, 1442, 1715
Tsp45I GTSAC 2 cut(s) 562, 991
TspDTI ATGAA 7 cut(s) 223, 351, 732, 956, 978, 1193, 1566
TspGWI ACGGA 2 cut(s) 344, 1545
TspRI CASTG 2 cut(s) 1000, 1591
XapI RAATTY 4 cut(s) 353, 653, 788, 1307
XceI RCATGY 2 cut(s) 570, 1750
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 3 cut(s) 75, 1596, 1617
ZrmI AGTACT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.