No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 43 |
| AccB1I | GGYRCC | 1 cut(s) | 43 |
| AccBSI | CCGCTC | 1 cut(s) | 475 |
| AccI | GTMKAC | 1 cut(s) | 351 |
| AciI | CCGC | 3 cut(s) | 212, 475, 580 |
| AclWI | GGATC | 3 cut(s) | 73, 948, 1511 |
| AcoI | YGGCCR | 3 cut(s) | 1277, 1476, 2367 |
| AcsI | RAATTY | 4 cut(s) | 290, 1079, 1913, 2053 |
| AcvI | CACGTG | 1 cut(s) | 1206 |
| AfaI | GTAC | 7 cut(s) | 45, 196, 667, 964, 1090, 2063, 2069 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 866 |
| AflIII | ACRYGT | 2 cut(s) | 959, 1975 |
| AgsI | TTSAA | 8 cut(s) | 878, 1084, 1244, 1621, 1696, 1712, 1919, 2078 |
| AhlI | ACTAGT | 2 cut(s) | 2222, 2234 |
| Alw21I | GWGCWC | 1 cut(s) | 1211 |
| Alw26I | GTCTC | 5 cut(s) | 710, 776, 1217, 1588, 1794 |
| Alw44I | GTGCAC | 1 cut(s) | 1207 |
| AlwI | GGATC | 3 cut(s) | 73, 948, 1511 |
| Ama87I | CYCGRG | 2 cut(s) | 1730, 2282 |
| AoxI | GGCC | 4 cut(s) | 21, 1277, 1476, 2367 |
| ApaLI | GTGCAC | 1 cut(s) | 1207 |
| ApeKI | GCWGC | 2 cut(s) | 2048, 2321 |
| ApoI | RAATTY | 4 cut(s) | 290, 1079, 1913, 2053 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1772, 1804 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1563 |
| Asp718I | GGTACC | 1 cut(s) | 43 |
| AspLEI | GCGC | 1 cut(s) | 2281 |
| AspS9I | GGNCC | 2 cut(s) | 22, 1525 |
| AsuHPI | GGTGA | 3 cut(s) | 875, 1001, 1483 |
| AvaI | CYCGRG | 2 cut(s) | 1730, 2282 |
| AvaII | GGWCC | 1 cut(s) | 1525 |
| AxyI | CCTNAGG | 1 cut(s) | 163 |
| BaeGI | GKGCMC | 1 cut(s) | 1211 |
| BalI | TGGCCA | 2 cut(s) | 1279, 2369 |
| BanI | GGYRCC | 1 cut(s) | 43 |
| BauI | CACGAG | 1 cut(s) | 479 |
| BbrPI | CACGTG | 1 cut(s) | 1206 |
| BbsI | GAAGAC | 1 cut(s) | 2295 |
| Bbv12I | GWGCWC | 1 cut(s) | 1211 |
| BbvI | GCAGC | 2 cut(s) | 2060, 2308 |
| BccI | CCATC | 7 cut(s) | 34, 137, 1141, 1166, 1269, 1468, 1516 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2263, 2297 |
| BciVI | GTATCC | 1 cut(s) | 1681 |
| BcoDI | GTCTC | 5 cut(s) | 710, 776, 1217, 1588, 1794 |
| BcuI | ACTAGT | 2 cut(s) | 2222, 2234 |
| BfaI | CTAG | 4 cut(s) | 396, 402, 2223, 2235 |
| BfoI | RGCGCY | 1 cut(s) | 2282 |
| BfuI | GTATCC | 1 cut(s) | 1681 |
| BglII | AGATCT | 1 cut(s) | 1393 |
| BisI | GCNGC | 4 cut(s) | 212, 476, 2049, 2322 |
| BlsI | GCNGC | 4 cut(s) | 213, 477, 2050, 2323 |
| BmcAI | AGTACT | 1 cut(s) | 2069 |
| Bme18I | GGWCC | 1 cut(s) | 1525 |
| BmeT110I | CYCGRG | 2 cut(s) | 1730, 2282 |
| BmgT120I | GGNCC | 2 cut(s) | 22, 1525 |
| BmiI | GGNNCC | 3 cut(s) | 24, 45, 1527 |
| BmrI | ACTGGG | 1 cut(s) | 1465 |
| BmsI | GCATC | 3 cut(s) | 504, 1750, 2261 |
| BmuI | ACTGGG | 1 cut(s) | 1465 |
| BpiI | GAAGAC | 1 cut(s) | 2295 |
| BpmI | CTGGAG | 3 cut(s) | 491, 1602, 2155 |
| BpuEI | CTTGAG | 3 cut(s) | 1592, 1776, 2063 |
| Bsa29I | ATCGAT | 1 cut(s) | 135 |
| BsaAI | YACGTR | 2 cut(s) | 1206, 2360 |
| BsaBI | GATNNNNATC | 1 cut(s) | 77 |
| BsaI | GGTCTC | 1 cut(s) | 776 |
| BsaJI | CCNNGG | 3 cut(s) | 886, 1280, 1406 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1058, 1088, 2153, 2183 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 866 |
| Bse1I | ACTGG | 4 cut(s) | 602, 1460, 1585, 1969 |
| Bse21I | CCTNAGG | 1 cut(s) | 163 |
| Bse3DI | GCAATG | 2 cut(s) | 1510, 1770 |
| Bse8I | GATNNNNATC | 1 cut(s) | 77 |
| BseCI | ATCGAT | 1 cut(s) | 135 |
| BseDI | CCNNGG | 3 cut(s) | 886, 1280, 1406 |
| BseGI | GGATG | 5 cut(s) | 45, 859, 982, 1177, 1345 |
| BseJI | GATNNNNATC | 1 cut(s) | 77 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 866 |
| BseMI | GCAATG | 2 cut(s) | 1510, 1770 |
| BseMII | CTCAG | 4 cut(s) | 46, 814, 840, 1589 |
| BseNI | ACTGG | 4 cut(s) | 602, 1460, 1585, 1969 |
| BseRI | GAGGAG | 3 cut(s) | 1199, 1718, 1820 |
| BseSI | GKGCMC | 1 cut(s) | 1211 |
| BseXI | GCAGC | 2 cut(s) | 2060, 2308 |
| BshFI | GGCC | 4 cut(s) | 23, 1279, 1478, 2369 |
| BshNI | GGYRCC | 1 cut(s) | 43 |
| BshVI | ATCGAT | 1 cut(s) | 135 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1211 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1730, 2282 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 866 |
| BsmAI | GTCTC | 5 cut(s) | 710, 776, 1217, 1588, 1794 |
| BsnI | GGCC | 4 cut(s) | 23, 1279, 1478, 2369 |
| Bso31I | GGTCTC | 1 cut(s) | 776 |
| BsoBI | CYCGRG | 2 cut(s) | 1730, 2282 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1211 |
| Bsp143I | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| BspACI | CCGC | 3 cut(s) | 212, 475, 580 |
| BspANI | GGCC | 4 cut(s) | 23, 1279, 1478, 2369 |
| BspCNI | CTCAG | 4 cut(s) | 45, 813, 841, 1590 |
| BspDI | ATCGAT | 1 cut(s) | 135 |
| BspLI | GGNNCC | 3 cut(s) | 24, 45, 1527 |
| BspPI | GGATC | 3 cut(s) | 73, 948, 1511 |
| BspT107I | GGYRCC | 1 cut(s) | 43 |
| BspTNI | GGTCTC | 1 cut(s) | 776 |
| BsrBI | CCGCTC | 1 cut(s) | 475 |
| BsrDI | GCAATG | 2 cut(s) | 1510, 1770 |
| BsrI | ACTGG | 4 cut(s) | 602, 1460, 1585, 1969 |
| BssECI | CCNNGG | 3 cut(s) | 886, 1280, 1406 |
| BssMI | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| BssNAI | GTATAC | 1 cut(s) | 352 |
| BssSI | CACGAG | 1 cut(s) | 479 |
| BssT1I | CCWWGG | 3 cut(s) | 886, 1280, 1406 |
| Bst1107I | GTATAC | 1 cut(s) | 352 |
| Bst2BI | CACGAG | 1 cut(s) | 479 |
| Bst6I | CTCTTC | 5 cut(s) | 1155, 1257, 1281, 1616, 2259 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1957 |
| BstBAI | YACGTR | 2 cut(s) | 1206, 2360 |
| BstC8I | GCNNGC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 9 cut(s) | 32, 163, 780, 800, 849, 1098, 1227, 1598, 2373 |
| BstF5I | GGATG | 5 cut(s) | 45, 859, 982, 1177, 1345 |
| BstH2I | RGCGCY | 1 cut(s) | 2282 |
| BstHHI | GCGC | 1 cut(s) | 2281 |
| BstKTI | GATC | 9 cut(s) | 81, 567, 895, 943, 1396, 1483, 1519, 1750, 1948 |
| BstMAI | GTCTC | 5 cut(s) | 710, 776, 1217, 1588, 1794 |
| BstMBI | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 475, 492, 607, 1509, 1849, 1957, 2090, 2327 |
| BstNSI | RCATGY | 4 cut(s) | 963, 1193, 1979, 2356 |
| BstSLI | GKGCMC | 1 cut(s) | 1211 |
| BstV1I | GCAGC | 2 cut(s) | 2060, 2308 |
| BstV2I | GAAGAC | 1 cut(s) | 2295 |
| BstX2I | RGATCY | 2 cut(s) | 78, 1393 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1407 |
| BstYI | RGATCY | 2 cut(s) | 78, 1393 |
| BstZ17I | GTATAC | 1 cut(s) | 352 |
| Bsu15I | ATCGAT | 1 cut(s) | 135 |
| Bsu36I | CCTNAGG | 1 cut(s) | 163 |
| BsuI | GTATCC | 1 cut(s) | 1681 |
| BsuRI | GGCC | 4 cut(s) | 23, 1279, 1478, 2369 |
| BsuTUI | ATCGAT | 1 cut(s) | 135 |
| BtsCI | GGATG | 5 cut(s) | 45, 859, 982, 1177, 1345 |
| BtsI | GCAGTG | 3 cut(s) | 1104, 1519, 2304 |
| BtsIMutI | CAGTG | 7 cut(s) | 609, 1104, 1310, 1519, 1578, 2197, 2304 |
| Cac8I | GCNNGC | 1 cut(s) | 246 |
| CfoI | GCGC | 1 cut(s) | 2281 |
| Cfr13I | GGNCC | 2 cut(s) | 22, 1525 |
| ClaI | ATCGAT | 1 cut(s) | 135 |
| Csp6I | GTAC | 7 cut(s) | 44, 195, 666, 963, 1089, 2062, 2068 |
| CviAII | CATG | 8 cut(s) | 358, 960, 1190, 1435, 1715, 1976, 2249, 2353 |
| CviQI | GTAC | 7 cut(s) | 44, 195, 666, 963, 1089, 2062, 2068 |
| DdeI | CTNAG | 9 cut(s) | 32, 163, 780, 800, 849, 1098, 1227, 1598, 2373 |
| DpnI | GATC | 9 cut(s) | 80, 566, 894, 942, 1395, 1482, 1518, 1749, 1947 |
| DpnII | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| DraI | TTTAAA | 1 cut(s) | 822 |
| EaeI | YGGCCR | 3 cut(s) | 1277, 1476, 2367 |
| Eam1104I | CTCTTC | 5 cut(s) | 1155, 1257, 1281, 1616, 2259 |
| EarI | CTCTTC | 5 cut(s) | 1155, 1257, 1281, 1616, 2259 |
| Eco130I | CCWWGG | 3 cut(s) | 886, 1280, 1406 |
| Eco31I | GGTCTC | 1 cut(s) | 776 |
| Eco32I | GATATC | 1 cut(s) | 171 |
| Eco47I | GGWCC | 1 cut(s) | 1525 |
| Eco72I | CACGTG | 1 cut(s) | 1206 |
| Eco81I | CCTNAGG | 1 cut(s) | 163 |
| Eco88I | CYCGRG | 2 cut(s) | 1730, 2282 |
| EcoRI | GAATTC | 1 cut(s) | 1079 |
| EcoRV | GATATC | 1 cut(s) | 171 |
| EcoT14I | CCWWGG | 3 cut(s) | 886, 1280, 1406 |
| EcoT22I | ATGCAT | 1 cut(s) | 497 |
| ErhI | CCWWGG | 3 cut(s) | 886, 1280, 1406 |
| FaeI | CATG | 8 cut(s) | 361, 963, 1193, 1438, 1718, 1979, 2252, 2356 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1551, 1583 |
| FatI | CATG | 8 cut(s) | 357, 959, 1189, 1434, 1714, 1975, 2248, 2352 |
| FauNDI | CATATG | 1 cut(s) | 1071 |
| FblI | GTMKAC | 1 cut(s) | 351 |
| Fnu4HI | GCNGC | 4 cut(s) | 212, 476, 2049, 2322 |
| FokI | GGATG | 5 cut(s) | 52, 866, 989, 1184, 1352 |
| Fsp4HI | GCNGC | 4 cut(s) | 212, 476, 2049, 2322 |
| FspBI | CTAG | 4 cut(s) | 396, 402, 2223, 2235 |
| GlaI | GCGC | 1 cut(s) | 2280 |
| GluI | GCNGC | 4 cut(s) | 212, 476, 2049, 2322 |
| GsuI | CTGGAG | 3 cut(s) | 491, 1602, 2155 |
| HaeII | RGCGCY | 1 cut(s) | 2282 |
| HaeIII | GGCC | 4 cut(s) | 23, 1279, 1478, 2369 |
| HhaI | GCGC | 1 cut(s) | 2281 |
| Hin1II | CATG | 8 cut(s) | 361, 963, 1193, 1438, 1718, 1979, 2252, 2356 |
| Hin6I | GCGC | 1 cut(s) | 2279 |
| HinP1I | GCGC | 1 cut(s) | 2279 |
| HincII | GTYRAC | 1 cut(s) | 185 |
| HindII | GTYRAC | 1 cut(s) | 185 |
| HindIII | AAGCTT | 3 cut(s) | 1794, 1857, 2312 |
| HinfI | GANTC | 7 cut(s) | 125, 740, 953, 980, 1200, 1814, 2104 |
| HphI | GGTGA | 3 cut(s) | 875, 1001, 1483 |
| Hpy166II | GTNNAC | 7 cut(s) | 185, 327, 352, 883, 957, 1209, 2232 |
| Hpy188III | TCNNGA | 4 cut(s) | 510, 1197, 1571, 1751 |
| Hpy8I | GTNNAC | 7 cut(s) | 185, 327, 352, 883, 957, 1209, 2232 |
| HpyAV | CCTTC | 2 cut(s) | 7, 1690 |
| HpyCH4IV | ACGT | 2 cut(s) | 1205, 2359 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 475, 492, 607, 1509, 1849, 1957, 2090, 2327 |
| HpyF3I | CTNAG | 9 cut(s) | 32, 163, 780, 800, 849, 1098, 1227, 1598, 2373 |
| HpySE526I | ACGT | 2 cut(s) | 1205, 2359 |
| Hsp92II | CATG | 8 cut(s) | 361, 963, 1193, 1438, 1718, 1979, 2252, 2356 |
| HspAI | GCGC | 1 cut(s) | 2279 |
| KpnI | GGTACC | 1 cut(s) | 47 |
| Kzo9I | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| LmnI | GCTCC | 8 cut(s) | 241, 472, 796, 1066, 1186, 1642, 1753, 1885 |
| Lsp1109I | GCAGC | 2 cut(s) | 2060, 2308 |
| LweI | GCATC | 3 cut(s) | 504, 1750, 2261 |
| MaeI | CTAG | 4 cut(s) | 396, 402, 2223, 2235 |
| MaeII | ACGT | 2 cut(s) | 1205, 2359 |
| MaeIII | GTNAC | 7 cut(s) | 728, 1358, 2150, 2186, 2192, 2355, 2360 |
| MalI | GATC | 9 cut(s) | 80, 566, 894, 942, 1395, 1482, 1518, 1749, 1947 |
| MbiI | CCGCTC | 1 cut(s) | 475 |
| MboI | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| MfeI | CAATTG | 3 cut(s) | 901, 1153, 1466 |
| MflI | RGATCY | 2 cut(s) | 78, 1393 |
| MhlI | GDGCHC | 1 cut(s) | 1211 |
| MlsI | TGGCCA | 2 cut(s) | 1279, 2369 |
| MluNI | TGGCCA | 2 cut(s) | 1279, 2369 |
| MlyI | GAGTC | 3 cut(s) | 734, 962, 989 |
| MmeI | TCCRAC | 3 cut(s) | 219, 790, 978 |
| Mox20I | TGGCCA | 2 cut(s) | 1279, 2369 |
| Mph1103I | ATGCAT | 1 cut(s) | 497 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1563 |
| MscI | TGGCCA | 2 cut(s) | 1279, 2369 |
| MseI | TTAA | 3 cut(s) | 432, 821, 1854 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1014, 2247 |
| Msp20I | TGGCCA | 2 cut(s) | 1279, 2369 |
| MspA1I | CMGCKG | 1 cut(s) | 1538 |
| MunI | CAATTG | 3 cut(s) | 901, 1153, 1466 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 475, 492, 607, 1509, 1849, 1957, 2090, 2327 |
| NdeI | CATATG | 1 cut(s) | 1071 |
| NdeII | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| NlaIII | CATG | 8 cut(s) | 361, 963, 1193, 1438, 1718, 1979, 2252, 2356 |
| NlaIV | GGNNCC | 3 cut(s) | 24, 45, 1527 |
| NmuCI | GTSAC | 2 cut(s) | 2192, 2360 |
| NsiI | ATGCAT | 1 cut(s) | 497 |
| NspI | RCATGY | 4 cut(s) | 963, 1193, 1979, 2356 |
| PaeR7I | CTCGAG | 2 cut(s) | 1730, 2282 |
| PciI | ACATGT | 2 cut(s) | 959, 1975 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1202 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1563 |
| PfeI | GAWTC | 4 cut(s) | 125, 1200, 1814, 2104 |
| PkrI | GCNGC | 4 cut(s) | 213, 477, 2050, 2323 |
| PleI | GAGTC | 3 cut(s) | 734, 961, 988 |
| PmaCI | CACGTG | 1 cut(s) | 1206 |
| PmlI | CACGTG | 1 cut(s) | 1206 |
| PpsI | GAGTC | 3 cut(s) | 734, 961, 988 |
| Ppu21I | YACGTR | 2 cut(s) | 1206, 2360 |
| PscI | ACATGT | 2 cut(s) | 959, 1975 |
| PspCI | CACGTG | 1 cut(s) | 1206 |
| PspN4I | GGNNCC | 3 cut(s) | 24, 45, 1527 |
| PspPI | GGNCC | 2 cut(s) | 22, 1525 |
| PspXI | VCTCGAGB | 2 cut(s) | 1730, 2282 |
| PsuI | RGATCY | 2 cut(s) | 78, 1393 |
| PvuII | CAGCTG | 1 cut(s) | 1538 |
| RsaI | GTAC | 7 cut(s) | 45, 196, 667, 964, 1090, 2063, 2069 |
| RsaNI | GTAC | 7 cut(s) | 44, 195, 666, 963, 1089, 2062, 2068 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1014, 2247 |
| SaqAI | TTAA | 3 cut(s) | 432, 821, 1854 |
| SatI | GCNGC | 4 cut(s) | 212, 476, 2049, 2322 |
| Sau3AI | GATC | 9 cut(s) | 78, 564, 892, 940, 1393, 1480, 1516, 1747, 1945 |
| Sau96I | GGNCC | 2 cut(s) | 22, 1525 |
| ScaI | AGTACT | 1 cut(s) | 2069 |
| SchI | GAGTC | 3 cut(s) | 734, 962, 989 |
| SduI | GDGCHC | 1 cut(s) | 1211 |
| SfaNI | GCATC | 3 cut(s) | 504, 1750, 2261 |
| Sfr274I | CTCGAG | 2 cut(s) | 1730, 2282 |
| SinI | GGWCC | 1 cut(s) | 1525 |
| SlaI | CTCGAG | 2 cut(s) | 1730, 2282 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1014, 2247 |
| SmlI | CTYRAG | 5 cut(s) | 1571, 1730, 1791, 2042, 2282 |
| SmoI | CTYRAG | 5 cut(s) | 1571, 1730, 1791, 2042, 2282 |
| SpeI | ACTAGT | 2 cut(s) | 2222, 2234 |
| SsiI | CCGC | 3 cut(s) | 212, 475, 580 |
| SspMI | CTAG | 4 cut(s) | 396, 402, 2223, 2235 |
| StyI | CCWWGG | 3 cut(s) | 886, 1280, 1406 |
| TaiI | ACGT | 2 cut(s) | 1208, 2362 |
| TaqI | TCGA | 6 cut(s) | 135, 660, 1731, 1773, 2283, 2347 |
| TaqII | GACCGA | 1 cut(s) | 135 |
| TatI | WGTACW | 2 cut(s) | 665, 2067 |
| TauI | GCSGC | 2 cut(s) | 214, 478 |
| TfiI | GAWTC | 4 cut(s) | 125, 1200, 1814, 2104 |
| Tru1I | TTAA | 3 cut(s) | 432, 821, 1854 |
| Tru9I | TTAA | 3 cut(s) | 432, 821, 1854 |
| TscAI | CASTG | 7 cut(s) | 609, 1111, 1317, 1519, 1585, 2197, 2311 |
| TseFI | GTSAC | 2 cut(s) | 2192, 2360 |
| TseI | GCWGC | 2 cut(s) | 2048, 2321 |
| Tsp45I | GTSAC | 2 cut(s) | 2192, 2360 |
| TspRI | CASTG | 7 cut(s) | 609, 1111, 1317, 1519, 1585, 2197, 2311 |
| VneI | GTGCAC | 1 cut(s) | 1207 |
| VpaK11BI | GGWCC | 1 cut(s) | 1525 |
| XapI | RAATTY | 4 cut(s) | 290, 1079, 1913, 2053 |
| XceI | RCATGY | 4 cut(s) | 963, 1193, 1979, 2356 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 222 |
| XhoI | CTCGAG | 2 cut(s) | 1730, 2282 |
| XmiI | GTMKAC | 1 cut(s) | 351 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1563 |
| XspI | CTAG | 4 cut(s) | 396, 402, 2223, 2235 |
| ZrmI | AGTACT | 1 cut(s) | 2069 |
| Zsp2I | ATGCAT | 1 cut(s) | 497 |