Rroxscaffold_7G00216480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000977
Physical Location & Seq
Reverse (-)
67133789 .. 67139816
6028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00216480.1

Sequence Viewer

Length: 1752 bp
ATGGCCGATGTAAACTTCTATTTGGATTATAAATTGCAATCTTCAGTTTCAATAACTGCTACAGCAGAAGACTTCACCTTAACATACTCATGGCAAAGAAAAACTAACAAGGGAGAGGTTTTGGTTAAAAAGGTTGGTATATTAGAAAGACTGATGTTCTGGCTGGAGCGGCTCGTGGTCGTAGTATGCATCTTTTTGATATCCAGATTTGCTCGATTCTCTACTGTATTCTTCTGTTTTTCTGATTTCATCTACTTGATCTGCTTTGCTCTGCGGTTAGAGGTCAATCCTTTGCCAGTGGAAGTTGAAAAGGGTTTAAAAGCAGAGAAAAAGAAAGCCAAAATTGAGGATGAGCCACAAATAGGGAAGTTTGAAGTTCACCAAGGAGATCATAACAATTGGGAAAAGGAATTGGTGGCTAAATATAGGTTTATGGATCATATATCAGAGTCCACATGTACCAAAATTGAGGATGAGTCGCCAAAACAAAAGTTGGAAGTTCATCACCAAGATGAAAATAACAGTTGGGAAAAGGAATTGGTGGCAAAATATGGGTGTATGGAACATATGTCAGAATTCAAATGTACCAAACCTAAGGACATTGCCAAGTATGAAAATGAAGAAGAACCATCAAAAGATGAAACATACAATTGGGAAGAGGAATTGGCTGGGTTTATGGAGGAGCATGTATTGGGATTCACGTGCACCAAACCTAAGGAGACTAAGAAATCTGAAATTGAAGAAGAGGCACCAATAGAAGAGAATTACAGATGGCCAAGGGAAGAGGCCGGATTTGGAAGTTATCTTCCAAGTCCTTGGGATTATTTTGCTGCATATAAAGACATGGATAAAGTTGATGATAACTGGGGAAAACAATTGGTGATGGCAGATCAAATTGATACTGGCAATCACAATGGTAGCAGTGATCCGATGGAGACCACAACAGCTGAATATGAAAGCAATAAGGAAGCACTTCTTCTTGAGGACACTGGAGAAGTTGAGAGACTGAGAGCTGAAAATGATAATTTGAAGAGAGAAAAGGAAGCATTGGAGAGCGAAAAGGAAGCATTGGAGAGAGAAAAGGAAGCATTAGAGGATACATTGAAGGCAGAGATACTTAAACATGAAGATACTCTCCTCGAGTGGAAAATGCACGATCAGAAGCTTGATGCAATGCTTGTTGAAGCGAAGATGAAGTCTCAAGCTTTAGTTGAGGAGTTAGATTCTTACTCCAAGTTTGAGGAACAAATGCTGGAAACGCTTAAAGCTTACAAAAAAGATTGCAGAAAATGGGAGCAAGACAATAAAGACATTGGTGCAAAATTTGAAGCTGTAATTTCAGCTATTAGAAAAGATCAGCAGACTTTGCTAACTGGTCAAGGACATGTTTGCAAGTGCAAGGCAGAACGCAAGAAATGGGAAAATGAGAAAATCACACTCAATGAACAGCTTGAGGCAGTGAATTCTTGGTACGAGTACCATTTTGAAGTGGCAACGACAGAAAATTATTGGAATCAATACTATATGCAAAGGGAAAATTATTACAGTATTTGGTTGCGTTACTATGAGGACAACATCTACAGCCTTGTCAAAAGTAACAGTGACCTTGCTAAAAGGCTCGAGGTAGAAACTAGTTTGTCCGCTAGTTATCACAATCATGTTTTTGACTATGAAGAGAGATGCAGGGCGCTCGAGGAAGACAAGAACAATCGAGCTCACTGCAAAGCTTTGTGCTGCCAATGCCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

583

Amino Acids

68.81

Weight (kDa)

4.95

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 748
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 3 cut(s) 169, 274, 1641
AclWI GGATC 2 cut(s) 444, 920
AcoI YGGCCR 2 cut(s) 3, 773
AcsI RAATTY 3 cut(s) 575, 1322, 1462
AcuI CTGAAG 1 cut(s) 27
AcvI CACGTG 1 cut(s) 702
AfaI GTAC 4 cut(s) 460, 586, 1472, 1478
AfiI CCNNNNNNNGG 1 cut(s) 362
AflIII ACRYGT 2 cut(s) 455, 1384
AhlI ACTAGT 1 cut(s) 1631
Alw21I GWGCWC 2 cut(s) 707, 1717
Alw26I GTCTC 4 cut(s) 713, 929, 997, 1203
Alw44I GTGCAC 1 cut(s) 703
AlwI GGATC 2 cut(s) 444, 920
Ama87I CYCGRG 3 cut(s) 1139, 1619, 1691
AoxI GGCC 3 cut(s) 3, 773, 786
ApaLI GTGCAC 1 cut(s) 703
ApeKI GCWGC 2 cut(s) 830, 1734
ApoI RAATTY 3 cut(s) 575, 1322, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
Asp700I GAANNNNTTC 1 cut(s) 972
AspLEI GCGC 1 cut(s) 1690
AsuHPI GGTGA 4 cut(s) 67, 371, 497, 892
AvaI CYCGRG 3 cut(s) 1139, 1619, 1691
AxyI CCTNAGG 2 cut(s) 594, 714
BaeGI GKGCMC 1 cut(s) 707
BalI TGGCCA 1 cut(s) 775
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 1717
BauI CACGAG 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 702
BbsI GAAGAC 2 cut(s) 75, 1704
Bbv12I GWGCWC 2 cut(s) 707, 1717
BbvI GCAGC 2 cut(s) 817, 1721
BccI CCATC 4 cut(s) 637, 765, 877, 925
BcgI CGANNNNNNTGC 4 cut(s) 1672, 1701, 1706, 1735
BciVI GTATCC 1 cut(s) 1090
BcoDI GTCTC 4 cut(s) 713, 929, 997, 1203
BcuI ACTAGT 1 cut(s) 1631
BfaI CTAG 2 cut(s) 1632, 1644
BfmI CTRYAG 2 cut(s) 60, 1579
BfoI RGCGCY 1 cut(s) 1691
BfuI GTATCC 1 cut(s) 1090
BisI GCNGC 3 cut(s) 170, 831, 1735
BlsI GCNGC 3 cut(s) 171, 832, 1736
BmeT110I CYCGRG 3 cut(s) 1139, 1619, 1691
BmiI GGNNCC 1 cut(s) 750
BmrI ACTGGG 1 cut(s) 874
BmsI GCATC 3 cut(s) 198, 1159, 1670
BmuI ACTGGG 1 cut(s) 874
BpiI GAAGAC 2 cut(s) 75, 1704
BpmI CTGGAG 2 cut(s) 185, 1011
BpuEI CTTGAG 3 cut(s) 1001, 1185, 1472
BsaAI YACGTR 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 3 cut(s) 382, 776, 815
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 5 cut(s) 296, 869, 907, 994, 1378
Bse21I CCTNAGG 2 cut(s) 594, 714
Bse3DI GCAATG 2 cut(s) 600, 1179
BseDI CCNNGG 3 cut(s) 382, 776, 815
BseGI GGATG 2 cut(s) 355, 478
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMI GCAATG 2 cut(s) 600, 1179
BseMII CTCAG 1 cut(s) 998
BseNI ACTGG 5 cut(s) 296, 869, 907, 994, 1378
BseRI GAGGAG 3 cut(s) 695, 1127, 1229
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 2 cut(s) 817, 1721
BseYI CCCAGC 1 cut(s) 668
BshFI GGCC 3 cut(s) 5, 775, 788
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 707, 1717
BsiHKCI CYCGRG 3 cut(s) 1139, 1619, 1691
BsiSI CCGG 1 cut(s) 789
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 4 cut(s) 713, 929, 997, 1203
BsnI GGCC 3 cut(s) 5, 775, 788
Bso31I GGTCTC 1 cut(s) 929
BsoBI CYCGRG 3 cut(s) 1139, 1619, 1691
Bsp1286I GDGCHC 2 cut(s) 707, 1717
Bsp143I GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
BspACI CCGC 3 cut(s) 169, 274, 1641
BspANI GGCC 3 cut(s) 5, 775, 788
BspCNI CTCAG 1 cut(s) 999
BspLI GGNNCC 1 cut(s) 750
BspPI GGATC 2 cut(s) 444, 920
BspT107I GGYRCC 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 929
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 2 cut(s) 600, 1179
BsrI ACTGG 5 cut(s) 296, 869, 907, 994, 1378
BssECI CCNNGG 3 cut(s) 382, 776, 815
BssMI GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
BssSI CACGAG 1 cut(s) 173
BssT1I CCWWGG 3 cut(s) 382, 776, 815
Bst2BI CACGAG 1 cut(s) 173
Bst4CI ACNGT 4 cut(s) 226, 524, 1547, 1601
Bst6I CTCTTC 6 cut(s) 651, 738, 753, 777, 1025, 1668
BstAPI GCANNNNNTGC 1 cut(s) 1366
BstBAI YACGTR 1 cut(s) 702
BstDEI CTNAG 4 cut(s) 594, 714, 723, 1007
BstF5I GGATG 2 cut(s) 355, 478
BstH2I RGCGCY 1 cut(s) 1691
BstHHI GCGC 1 cut(s) 1690
BstKTI GATC 7 cut(s) 261, 391, 439, 892, 928, 1159, 1357
BstMAI GTCTC 4 cut(s) 713, 929, 997, 1203
BstMBI GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
BstMWI GCNNNNNNNGC 4 cut(s) 169, 1258, 1366, 1740
BstNSI RCATGY 3 cut(s) 459, 689, 1388
BstSFI CTRYAG 2 cut(s) 60, 1579
BstSLI GKGCMC 1 cut(s) 707
BstV1I GCAGC 2 cut(s) 817, 1721
BstV2I GAAGAC 2 cut(s) 75, 1704
BstXI CCANNNNNNTGG 1 cut(s) 816
Bsu36I CCTNAGG 2 cut(s) 594, 714
BsuI GTATCC 1 cut(s) 1090
BsuRI GGCC 3 cut(s) 5, 775, 788
BtsCI GGATG 2 cut(s) 355, 478
BtsI GCAGTG 3 cut(s) 928, 1464, 1717
BtsIMutI CAGTG 6 cut(s) 303, 928, 987, 1464, 1606, 1717
CfoI GCGC 1 cut(s) 1690
Csp6I GTAC 4 cut(s) 459, 585, 1471, 1477
CviAII CATG 7 cut(s) 90, 456, 686, 844, 1124, 1385, 1658
CviQI GTAC 4 cut(s) 459, 585, 1471, 1477
DdeI CTNAG 4 cut(s) 594, 714, 723, 1007
DpnI GATC 7 cut(s) 260, 390, 438, 891, 927, 1158, 1356
DpnII GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 2 cut(s) 3, 773
Eam1104I CTCTTC 6 cut(s) 651, 738, 753, 777, 1025, 1668
EarI CTCTTC 6 cut(s) 651, 738, 753, 777, 1025, 1668
Ecl136II GAGCTC 1 cut(s) 1715
Eco130I CCWWGG 3 cut(s) 382, 776, 815
Eco24I GRGCYC 1 cut(s) 1717
Eco31I GGTCTC 1 cut(s) 929
Eco32I GATATC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 1715
Eco57I CTGAAG 1 cut(s) 27
Eco72I CACGTG 1 cut(s) 702
Eco81I CCTNAGG 2 cut(s) 594, 714
Eco88I CYCGRG 3 cut(s) 1139, 1619, 1691
EcoICRI GAGCTC 1 cut(s) 1715
EcoRI GAATTC 2 cut(s) 575, 1462
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 3 cut(s) 382, 776, 815
EcoT22I ATGCAT 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 1717
ErhI CCWWGG 3 cut(s) 382, 776, 815
FaeI CATG 7 cut(s) 93, 459, 689, 847, 1127, 1388, 1661
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FatI CATG 7 cut(s) 89, 455, 685, 843, 1123, 1384, 1657
FauNDI CATATG 1 cut(s) 567
Fnu4HI GCNGC 3 cut(s) 170, 831, 1735
FokI GGATG 2 cut(s) 362, 485
FriOI GRGCYC 1 cut(s) 1717
Fsp4HI GCNGC 3 cut(s) 170, 831, 1735
FspBI CTAG 2 cut(s) 1632, 1644
GlaI GCGC 1 cut(s) 1689
GluI GCNGC 3 cut(s) 170, 831, 1735
GsaI CCCAGC 1 cut(s) 672
GsuI CTGGAG 2 cut(s) 185, 1011
HaeII RGCGCY 1 cut(s) 1691
HaeIII GGCC 3 cut(s) 5, 775, 788
HapII CCGG 1 cut(s) 789
HhaI GCGC 1 cut(s) 1690
Hin1II CATG 7 cut(s) 93, 459, 689, 847, 1127, 1388, 1661
Hin6I GCGC 1 cut(s) 1688
HinP1I GCGC 1 cut(s) 1688
HindIII AAGCTT 4 cut(s) 1163, 1203, 1266, 1725
HinfI GANTC 6 cut(s) 216, 449, 476, 696, 1223, 1513
HpaII CCGG 1 cut(s) 789
HphI GGTGA 4 cut(s) 67, 371, 497, 892
Hpy166II GTNNAC 4 cut(s) 13, 379, 453, 705
Hpy188I TCNGA 7 cut(s) 244, 448, 574, 733, 930, 1161, 1751
Hpy188III TCNNGA 2 cut(s) 204, 980
Hpy8I GTNNAC 4 cut(s) 13, 379, 453, 705
HpyAV CCTTC 1 cut(s) 1099
HpyCH4III ACNGT 4 cut(s) 226, 524, 1547, 1601
HpyCH4IV ACGT 1 cut(s) 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 1258, 1366, 1740
HpyF3I CTNAG 4 cut(s) 594, 714, 723, 1007
HpySE526I ACGT 1 cut(s) 701
Hsp92II CATG 7 cut(s) 93, 459, 689, 847, 1127, 1388, 1661
HspAI GCGC 1 cut(s) 1688
Kzo9I GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
LmnI GCTCC 3 cut(s) 166, 682, 1294
Lsp1109I GCAGC 2 cut(s) 817, 1721
LweI GCATC 3 cut(s) 198, 1159, 1670
MaeI CTAG 2 cut(s) 1632, 1644
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 3 cut(s) 1559, 1595, 1601
MalI GATC 7 cut(s) 260, 390, 438, 891, 927, 1158, 1356
MbiI CCGCTC 1 cut(s) 169
MboI GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
MfeI CAATTG 3 cut(s) 397, 649, 875
MhlI GDGCHC 2 cut(s) 707, 1717
MlsI TGGCCA 1 cut(s) 775
MluNI TGGCCA 1 cut(s) 775
MlyI GAGTC 2 cut(s) 458, 485
MmeI TCCRAC 1 cut(s) 474
Mox20I TGGCCA 1 cut(s) 775
Mph1103I ATGCAT 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 972
MscI TGGCCA 1 cut(s) 775
MseI TTAA 5 cut(s) 80, 126, 317, 1119, 1263
MslI CAYNNNNRTG 3 cut(s) 88, 510, 1656
Msp20I TGGCCA 1 cut(s) 775
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 1 cut(s) 789
MunI CAATTG 3 cut(s) 397, 649, 875
MwoI GCNNNNNNNGC 4 cut(s) 169, 1258, 1366, 1740
NdeI CATATG 1 cut(s) 567
NdeII GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
NlaIII CATG 7 cut(s) 93, 459, 689, 847, 1127, 1388, 1661
NlaIV GGNNCC 1 cut(s) 750
NmuCI GTSAC 1 cut(s) 1601
NsiI ATGCAT 1 cut(s) 191
NspI RCATGY 3 cut(s) 459, 689, 1388
PaeR7I CTCGAG 3 cut(s) 1139, 1619, 1691
PciI ACATGT 2 cut(s) 455, 1384
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 4 cut(s) 216, 696, 1223, 1513
PkrI GCNGC 3 cut(s) 171, 832, 1736
PleI GAGTC 2 cut(s) 457, 484
PmaCI CACGTG 1 cut(s) 702
PmlI CACGTG 1 cut(s) 702
PpsI GAGTC 2 cut(s) 457, 484
Ppu21I YACGTR 1 cut(s) 702
PscI ACATGT 2 cut(s) 455, 1384
PsiI TTATAA 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 1717
PspCI CACGTG 1 cut(s) 702
PspFI CCCAGC 1 cut(s) 668
PspN4I GGNNCC 1 cut(s) 750
PspXI VCTCGAGB 3 cut(s) 1139, 1619, 1691
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 4 cut(s) 460, 586, 1472, 1478
RsaNI GTAC 4 cut(s) 459, 585, 1471, 1477
RseI CAYNNNNRTG 3 cut(s) 88, 510, 1656
SacI GAGCTC 1 cut(s) 1717
SaqAI TTAA 5 cut(s) 80, 126, 317, 1119, 1263
SatI GCNGC 3 cut(s) 170, 831, 1735
Sau3AI GATC 7 cut(s) 258, 388, 436, 889, 925, 1156, 1354
SchI GAGTC 2 cut(s) 458, 485
SduI GDGCHC 2 cut(s) 707, 1717
SfaNI GCATC 3 cut(s) 198, 1159, 1670
SfcI CTRYAG 2 cut(s) 60, 1579
Sfr274I CTCGAG 3 cut(s) 1139, 1619, 1691
SlaI CTCGAG 3 cut(s) 1139, 1619, 1691
SmiMI CAYNNNNRTG 3 cut(s) 88, 510, 1656
SmlI CTYRAG 6 cut(s) 980, 1139, 1200, 1451, 1619, 1691
SmoI CTYRAG 6 cut(s) 980, 1139, 1200, 1451, 1619, 1691
SpeI ACTAGT 1 cut(s) 1631
SsiI CCGC 3 cut(s) 169, 274, 1641
SspMI CTAG 2 cut(s) 1632, 1644
SstI GAGCTC 1 cut(s) 1717
StyI CCWWGG 3 cut(s) 382, 776, 815
TaaI ACNGT 4 cut(s) 226, 524, 1547, 1601
TaiI ACGT 1 cut(s) 704
TaqI TCGA 5 cut(s) 214, 1140, 1620, 1692, 1711
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 4 cut(s) 216, 696, 1223, 1513
Tru1I TTAA 5 cut(s) 80, 126, 317, 1119, 1263
Tru9I TTAA 5 cut(s) 80, 126, 317, 1119, 1263
TscAI CASTG 6 cut(s) 303, 928, 994, 1464, 1606, 1724
TseFI GTSAC 1 cut(s) 1601
TseI GCWGC 2 cut(s) 830, 1734
Tsp45I GTSAC 1 cut(s) 1601
TspRI CASTG 6 cut(s) 303, 928, 994, 1464, 1606, 1724
VneI GTGCAC 1 cut(s) 703
XapI RAATTY 3 cut(s) 575, 1322, 1462
XceI RCATGY 3 cut(s) 459, 689, 1388
XhoI CTCGAG 3 cut(s) 1139, 1619, 1691
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 2 cut(s) 1632, 1644
Zsp2I ATGCAT 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.