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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 63 |
| AfaI | GTAC | 1 cut(s) | 275 |
| AflIII | ACRYGT | 1 cut(s) | 113 |
| AgsI | TTSAA | 4 cut(s) | 23, 242, 368, 422 |
| AjnI | CCWGG | 1 cut(s) | 14 |
| AluBI | AGCT | 4 cut(s) | 106, 173, 329, 353 |
| AluI | AGCT | 4 cut(s) | 106, 173, 329, 353 |
| AoxI | GGCC | 1 cut(s) | 313 |
| ApeKI | GCWGC | 2 cut(s) | 32, 173 |
| ApoI | RAATTY | 1 cut(s) | 63 |
| AspS9I | GGNCC | 2 cut(s) | 12, 313 |
| AsuHPI | GGTGA | 1 cut(s) | 419 |
| AvaII | GGWCC | 1 cut(s) | 12 |
| BbvI | GCAGC | 2 cut(s) | 44, 160 |
| BciT130I | CCWGG | 1 cut(s) | 16 |
| BisI | GCNGC | 2 cut(s) | 33, 174 |
| BlsI | GCNGC | 2 cut(s) | 34, 175 |
| BmcAI | AGTACT | 1 cut(s) | 275 |
| Bme1390I | CCNGG | 1 cut(s) | 16 |
| Bme18I | GGWCC | 1 cut(s) | 12 |
| BmgT120I | GGNCC | 2 cut(s) | 12, 313 |
| BmrFI | CCNGG | 1 cut(s) | 16 |
| BsaJI | CCNNGG | 1 cut(s) | 430 |
| Bse1I | ACTGG | 2 cut(s) | 77, 158 |
| BseBI | CCWGG | 1 cut(s) | 16 |
| BseDI | CCNNGG | 1 cut(s) | 430 |
| BseGI | GGATG | 1 cut(s) | 403 |
| BseMII | CTCAG | 1 cut(s) | 358 |
| BseNI | ACTGG | 2 cut(s) | 77, 158 |
| BseXI | GCAGC | 2 cut(s) | 44, 160 |
| BshFI | GGCC | 1 cut(s) | 315 |
| BslFI | GGGAC | 1 cut(s) | 83 |
| BsmFI | GGGAC | 1 cut(s) | 83 |
| BsnI | GGCC | 1 cut(s) | 315 |
| BspANI | GGCC | 1 cut(s) | 315 |
| BspCNI | CTCAG | 1 cut(s) | 357 |
| BsrI | ACTGG | 2 cut(s) | 77, 158 |
| BssECI | CCNNGG | 1 cut(s) | 430 |
| BssT1I | CCWWGG | 1 cut(s) | 430 |
| Bst2UI | CCWGG | 1 cut(s) | 16 |
| Bst4CI | ACNGT | 1 cut(s) | 278 |
| BstDEI | CTNAG | 3 cut(s) | 203, 257, 344 |
| BstF5I | GGATG | 1 cut(s) | 403 |
| BstNI | CCWGG | 1 cut(s) | 16 |
| BstNSI | RCATGY | 1 cut(s) | 117 |
| BstSCI | CCNGG | 1 cut(s) | 14 |
| BstV1I | GCAGC | 2 cut(s) | 44, 160 |
| BsuRI | GGCC | 1 cut(s) | 315 |
| BtsCI | GGATG | 1 cut(s) | 403 |
| Cfr13I | GGNCC | 2 cut(s) | 12, 313 |
| Csp6I | GTAC | 1 cut(s) | 274 |
| CviAII | CATG | 1 cut(s) | 114 |
| CviJI | RGCY | 7 cut(s) | 106, 173, 315, 329, 353, 386, 403 |
| CviKI_1 | RGCY | 7 cut(s) | 106, 173, 315, 329, 353, 386, 403 |
| CviQI | GTAC | 1 cut(s) | 274 |
| DdeI | CTNAG | 3 cut(s) | 203, 257, 344 |
| Eco130I | CCWWGG | 1 cut(s) | 430 |
| Eco47I | GGWCC | 1 cut(s) | 12 |
| EcoO109I | RGGNCCY | 1 cut(s) | 313 |
| EcoRII | CCWGG | 1 cut(s) | 14 |
| EcoT14I | CCWWGG | 1 cut(s) | 430 |
| ErhI | CCWWGG | 1 cut(s) | 430 |
| FaeI | CATG | 1 cut(s) | 117 |
| FaiI | YATR | 6 cut(s) | 115, 190, 192, 212, 291, 320 |
| FaqI | GGGAC | 1 cut(s) | 83 |
| FatI | CATG | 1 cut(s) | 113 |
| Fnu4HI | GCNGC | 2 cut(s) | 33, 174 |
| FokI | GGATG | 1 cut(s) | 410 |
| Fsp4HI | GCNGC | 2 cut(s) | 33, 174 |
| GluI | GCNGC | 2 cut(s) | 33, 174 |
| HaeIII | GGCC | 1 cut(s) | 315 |
| Hin1II | CATG | 1 cut(s) | 117 |
| HindIII | AAGCTT | 1 cut(s) | 327 |
| HinfI | GANTC | 3 cut(s) | 19, 93, 199 |
| HphI | GGTGA | 1 cut(s) | 419 |
| Hpy166II | GTNNAC | 1 cut(s) | 427 |
| Hpy188I | TCNGA | 4 cut(s) | 9, 127, 151, 347 |
| Hpy188III | TCNNGA | 1 cut(s) | 196 |
| Hpy8I | GTNNAC | 1 cut(s) | 427 |
| Hpy99I | CGWCG | 1 cut(s) | 93 |
| HpyCH4III | ACNGT | 1 cut(s) | 278 |
| HpyCH4V | TGCA | 2 cut(s) | 100, 268 |
| HpyF3I | CTNAG | 3 cut(s) | 203, 257, 344 |
| Hsp92II | CATG | 1 cut(s) | 117 |
| LpnPI | CCDG | 5 cut(s) | 28, 58, 139, 181, 309 |
| Lsp1109I | GCAGC | 2 cut(s) | 44, 160 |
| MaeIII | GTNAC | 1 cut(s) | 278 |
| MluCI | AATT | 5 cut(s) | 63, 158, 224, 390, 458 |
| MnlI | CCTC | 7 cut(s) | 3, 30, 144, 188, 326, 352, 388 |
| MspA1I | CMGCKG | 1 cut(s) | 173 |
| MspR9I | CCNGG | 1 cut(s) | 16 |
| MvaI | CCWGG | 1 cut(s) | 16 |
| NlaIII | CATG | 1 cut(s) | 117 |
| NspI | RCATGY | 1 cut(s) | 117 |
| PciI | ACATGT | 1 cut(s) | 113 |
| PfeI | GAWTC | 3 cut(s) | 19, 93, 199 |
| PkrI | GCNGC | 2 cut(s) | 34, 175 |
| PscI | ACATGT | 1 cut(s) | 113 |
| Psp6I | CCWGG | 1 cut(s) | 14 |
| PspGI | CCWGG | 1 cut(s) | 14 |
| PspPI | GGNCC | 2 cut(s) | 12, 313 |
| PvuII | CAGCTG | 1 cut(s) | 173 |
| RsaI | GTAC | 1 cut(s) | 275 |
| RsaNI | GTAC | 1 cut(s) | 274 |
| SatI | GCNGC | 2 cut(s) | 33, 174 |
| Sau96I | GGNCC | 2 cut(s) | 12, 313 |
| ScaI | AGTACT | 1 cut(s) | 275 |
| ScrFI | CCNGG | 1 cut(s) | 16 |
| SetI | ASST | 4 cut(s) | 108, 175, 331, 355 |
| SinI | GGWCC | 1 cut(s) | 12 |
| Sse9I | AATT | 5 cut(s) | 63, 158, 224, 390, 458 |
| StyD4I | CCNGG | 1 cut(s) | 14 |
| StyI | CCWWGG | 1 cut(s) | 430 |
| TaaI | ACNGT | 1 cut(s) | 278 |
| TasI | AATT | 5 cut(s) | 63, 158, 224, 390, 458 |
| TatI | WGTACW | 1 cut(s) | 273 |
| TfiI | GAWTC | 3 cut(s) | 19, 93, 199 |
| TseI | GCWGC | 2 cut(s) | 32, 173 |
| VpaK11BI | GGWCC | 1 cut(s) | 12 |
| XapI | RAATTY | 1 cut(s) | 63 |
| XceI | RCATGY | 1 cut(s) | 117 |
| ZrmI | AGTACT | 1 cut(s) | 275 |