FvH4_2g21830

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000837
Physical Location & Seq
Reverse (-)
18042555 .. 18044308
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21830.t1

Sequence Viewer

Length: 1086 bp
ATGGAAGATGGATCAGAAAGTTATGACAGAGCCAAAGAAGTGAAGAAGTTTGATGAAACAAAAGCTGGAGTGAAAGGGCTTATAGACTCCGGAGTGACCAAAGTGCCAAAGTTCCTCATGCATCCACCAGAGAACCTTTCAAGTTCAAGCACTCCAACCAGTACAGACTTCCAAGTTCCAGTTATAGATCTTGAGGGGTTCCATGAGAATCTCCATCGGGTGCAGATTGTTAAGGAGATTCGTGAAGCATCGGAAACATGGGGGTTCTTTCAAATGGTTAATCATGGAGTTCCAAACACTGTTATAGACAATTTGTTACAAGCAATAAAAGAATTTCATGAGCAGCCAAAGGAGGTGAAGATGGAGTGGTACTCACGCGACTCTACGAAAAGGGTAAGATACTACTGCAATGGAGATCTGCTGGTGGCAAAAAGAGCAAACTGGAGGGATTCAATTGTTTTCGATTTCCAGGATGGACCTTTGGAACCTGAGGCATTTCCCTTGGTTTGCAGACAGGCAGTGCAAGAATACATGGAACACTTGATCAACCTAAGAGATGCGCTATCAAGTTTGTTGTCAGAGGCCTTGGGGATCAGCAGTGACTACCTAGCTAGCATAGAGTGCATGAAGGGTGAAAATCTGGTGTGCCATTATTACCCGGTATGTCCAGAACCAGACCTCACCATAGGGACAACCAAGCATTCAGATCCTTCATCTTTAACTATACTCTTACAAGACAACATCGGCGGCCTCCAAGTCCTCCACCAAGGTCACTGGATCGACGTTCCCCCTAGGCACGGAACCCTGGTGGCGAATATCGGTGATCTTATGCAGCTTATCACCAACGACAAGTTCAAAAGCGTGGAACATAGGGTGCTGGCAAGAAGGGTTGGTCCCAGGATATCAGCTGCATGCTTTCTCTATCCAAGTGCTACACATAGGTTCAAACCTTATGGTCCAATCAAGGAGCTTCTGTCCGGTGACTTACCTATATATAGGGAAACTCATCTGGGAGAATATCTTGGCTACTACAAATCAAAGGGTTTGGATGGTAACTCTGCCATTAGTCACTTTAAACTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.8

Weight (kDa)

5.93

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 157 1e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 307 9.5e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 89
AciI CCGC 1 cut(s) 747
AclWI GGATC 4 cut(s) 19, 599, 701, 785
AcsI RAATTY 1 cut(s) 332
AfaI GTAC 2 cut(s) 163, 371
AfiI CCNNNNNNNGG 1 cut(s) 797
AgsI TTSAA 6 cut(s) 141, 147, 272, 453, 856, 946
AjnI CCWGG 3 cut(s) 468, 804, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
AluBI AGCT 5 cut(s) 65, 611, 835, 908, 970
AluI AGCT 5 cut(s) 65, 611, 835, 908, 970
AlwI GGATC 4 cut(s) 19, 599, 701, 785
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 2 cut(s) 582, 748
ApeKI GCWGC 3 cut(s) 343, 832, 908
ApoI RAATTY 1 cut(s) 332
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 1 cut(s) 562
AspS9I GGNCC 3 cut(s) 476, 893, 956
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 6 cut(s) 367, 644, 673, 832, 833, 992
AsuNHI GCTAGC 1 cut(s) 611
AvaII GGWCC 3 cut(s) 476, 893, 956
AvrII CCTAGG 1 cut(s) 791
AxyI CCTNAGG 1 cut(s) 489
BbvI GCAGC 3 cut(s) 355, 844, 895
BccI CCATC 5 cut(s) 2, 222, 355, 467, 1043
BciT130I CCWGG 3 cut(s) 470, 806, 898
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 659
BfaI CTAG 3 cut(s) 608, 612, 792
BglII AGATCT 2 cut(s) 187, 415
BisI GCNGC 4 cut(s) 344, 748, 833, 909
BlnI CCTAGG 1 cut(s) 791
BlsI GCNGC 4 cut(s) 345, 749, 834, 910
Bme1390I CCNGG 4 cut(s) 470, 659, 806, 898
Bme18I GGWCC 3 cut(s) 476, 893, 956
BmgT120I GGNCC 3 cut(s) 476, 893, 956
BmiI GGNNCC 4 cut(s) 200, 486, 802, 895
BmrFI CCNGG 4 cut(s) 470, 659, 806, 898
BmsI GCATC 3 cut(s) 130, 257, 547
BmtI GCTAGC 1 cut(s) 615
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 2 cut(s) 87, 463
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 659
BsaJI CCNNGG 6 cut(s) 501, 585, 766, 791, 804, 896
BsaWI WCCGGW 2 cut(s) 89, 977
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 4 cut(s) 159, 179, 446, 779
Bse21I CCTNAGG 1 cut(s) 489
Bse3DI GCAATG 1 cut(s) 415
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 3 cut(s) 470, 806, 898
BseDI CCNNGG 6 cut(s) 501, 585, 766, 791, 804, 896
BseGI GGATG 3 cut(s) 121, 478, 1054
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 4 cut(s) 159, 179, 446, 779
BseXI GCAGC 3 cut(s) 355, 844, 895
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 2 cut(s) 584, 750
BsiSI CCGG 3 cut(s) 90, 659, 978
BslFI GGGAC 2 cut(s) 703, 879
BslI CCNNNNNNNGG 1 cut(s) 797
BsmFI GGGAC 2 cut(s) 703, 879
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 2 cut(s) 584, 750
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
BspACI CCGC 1 cut(s) 747
BspANI GGCC 2 cut(s) 584, 750
BspCNI CTCAG 1 cut(s) 481
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 4 cut(s) 200, 486, 802, 895
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 4 cut(s) 19, 599, 701, 785
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 4 cut(s) 159, 179, 446, 779
BssECI CCNNGG 6 cut(s) 501, 585, 766, 791, 804, 896
BssMI GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
BssT1I CCWWGG 4 cut(s) 501, 585, 766, 791
Bst2UI CCWGG 3 cut(s) 470, 806, 898
Bst4CI ACNGT 1 cut(s) 301
BstAPI GCANNNNNTGC 1 cut(s) 621
BstC8I GCNNGC 3 cut(s) 613, 879, 913
BstDEI CTNAG 2 cut(s) 489, 551
BstF5I GGATG 3 cut(s) 121, 478, 1054
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 8 cut(s) 14, 190, 418, 546, 594, 709, 780, 826
BstMBI GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
BstMWI GCNNNNNNNGC 2 cut(s) 434, 621
BstNI CCWGG 3 cut(s) 470, 806, 898
BstNSI RCATGY 1 cut(s) 915
BstSCI CCNGG 4 cut(s) 468, 657, 804, 896
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 3 cut(s) 355, 844, 895
BstX2I RGATCY 3 cut(s) 187, 415, 706
BstYI RGATCY 3 cut(s) 187, 415, 706
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 2 cut(s) 584, 750
BtsCI GGATG 3 cut(s) 121, 478, 1054
BtsI GCAGTG 2 cut(s) 525, 604
BtsIMutI CAGTG 4 cut(s) 297, 525, 604, 772
Cac8I GCNNGC 3 cut(s) 613, 879, 913
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 562
Cfr13I GGNCC 3 cut(s) 476, 893, 956
Csp6I GTAC 2 cut(s) 162, 370
CviAII CATG 8 cut(s) 118, 203, 258, 284, 338, 532, 625, 912
CviQI GTAC 2 cut(s) 162, 370
DdeI CTNAG 2 cut(s) 489, 551
DpnI GATC 8 cut(s) 13, 189, 417, 545, 593, 708, 779, 825
DpnII GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
DraI TTTAAA 1 cut(s) 1075
Eco130I CCWWGG 4 cut(s) 501, 585, 766, 791
Eco147I AGGCCT 1 cut(s) 584
Eco32I GATATC 1 cut(s) 903
Eco47I GGWCC 3 cut(s) 476, 893, 956
Eco81I CCTNAGG 1 cut(s) 489
EcoRII CCWGG 3 cut(s) 468, 804, 896
EcoRV GATATC 1 cut(s) 903
EcoT14I CCWWGG 4 cut(s) 501, 585, 766, 791
EcoT22I ATGCAT 1 cut(s) 123
ErhI CCWWGG 4 cut(s) 501, 585, 766, 791
FaeI CATG 8 cut(s) 121, 206, 261, 287, 341, 535, 628, 915
FaqI GGGAC 2 cut(s) 703, 879
FatI CATG 8 cut(s) 117, 202, 257, 283, 337, 531, 624, 911
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 4 cut(s) 344, 748, 833, 909
FokI GGATG 3 cut(s) 108, 485, 1061
Fsp4HI GCNGC 4 cut(s) 344, 748, 833, 909
FspBI CTAG 3 cut(s) 608, 612, 792
GlaI GCGC 1 cut(s) 561
GluI GCNGC 4 cut(s) 344, 748, 833, 909
GsuI CTGGAG 2 cut(s) 87, 463
HaeIII GGCC 2 cut(s) 584, 750
HapII CCGG 3 cut(s) 90, 659, 978
HhaI GCGC 1 cut(s) 562
Hin1II CATG 8 cut(s) 121, 206, 261, 287, 341, 535, 628, 915
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HinfI GANTC 5 cut(s) 86, 208, 238, 380, 449
HpaII CCGG 3 cut(s) 90, 659, 978
HphI GGTGA 6 cut(s) 367, 644, 673, 832, 833, 992
Hpy188I TCNGA 4 cut(s) 16, 253, 580, 706
Hpy188III TCNNGA 5 cut(s) 90, 191, 242, 338, 668
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 622, 720, 879
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 8 cut(s) 121, 223, 408, 510, 523, 624, 832, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 621
HpyF3I CTNAG 2 cut(s) 489, 551
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 8 cut(s) 121, 206, 261, 287, 341, 535, 628, 915
HspAI GCGC 1 cut(s) 560
Kpn2I TCCGGA 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
LmnI GCTCC 1 cut(s) 967
Lsp1109I GCAGC 3 cut(s) 355, 844, 895
LweI GCATC 3 cut(s) 130, 257, 547
MaeI CTAG 3 cut(s) 608, 612, 792
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 7 cut(s) 94, 315, 599, 770, 980, 1052, 1067
MalI GATC 8 cut(s) 13, 189, 417, 545, 593, 708, 779, 825
MboI GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
MboII GAAGA 3 cut(s) 17, 55, 370
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 3 cut(s) 187, 415, 706
MluCI AATT 3 cut(s) 310, 332, 453
MlyI GAGTC 2 cut(s) 80, 374
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 9 cut(s) 125, 187, 346, 438, 484, 574, 689, 761, 770
Mph1103I ATGCAT 1 cut(s) 123
MroI TCCGGA 1 cut(s) 89
MseI TTAA 4 cut(s) 231, 279, 719, 1074
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 3 cut(s) 90, 659, 978
MspR9I CCNGG 4 cut(s) 470, 659, 806, 898
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 3 cut(s) 470, 806, 898
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 2 cut(s) 434, 621
NciI CCSGG 1 cut(s) 659
NdeII GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 8 cut(s) 121, 206, 261, 287, 341, 535, 628, 915
NlaIV GGNNCC 4 cut(s) 200, 486, 802, 895
NmuCI GTSAC 5 cut(s) 94, 599, 770, 980, 1067
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 1 cut(s) 915
PaeI GCATGC 1 cut(s) 915
PagI TCATGA 1 cut(s) 337
PceI AGGCCT 1 cut(s) 584
PctI GAATGC 1 cut(s) 700
PfeI GAWTC 3 cut(s) 208, 238, 449
PfoI TCCNGGA 1 cut(s) 468
PkrI GCNGC 4 cut(s) 345, 749, 834, 910
PleI GAGTC 2 cut(s) 80, 374
PpsI GAGTC 2 cut(s) 80, 374
Psp6I CCWGG 3 cut(s) 468, 804, 896
PspGI CCWGG 3 cut(s) 468, 804, 896
PspN4I GGNNCC 4 cut(s) 200, 486, 802, 895
PspPI GGNCC 3 cut(s) 476, 893, 956
PsuI RGATCY 3 cut(s) 187, 415, 706
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 2 cut(s) 163, 371
RsaNI GTAC 2 cut(s) 162, 370
SaqAI TTAA 4 cut(s) 231, 279, 719, 1074
SatI GCNGC 4 cut(s) 344, 748, 833, 909
Sau3AI GATC 8 cut(s) 11, 187, 415, 543, 591, 706, 777, 823
Sau96I GGNCC 3 cut(s) 476, 893, 956
SchI GAGTC 2 cut(s) 80, 374
ScrFI CCNGG 4 cut(s) 470, 659, 806, 898
SfaNI GCATC 3 cut(s) 130, 257, 547
SinI GGWCC 3 cut(s) 476, 893, 956
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SphI GCATGC 1 cut(s) 915
Sse9I AATT 3 cut(s) 310, 332, 453
SseBI AGGCCT 1 cut(s) 584
SsiI CCGC 1 cut(s) 747
SspMI CTAG 3 cut(s) 608, 612, 792
StuI AGGCCT 1 cut(s) 584
StyD4I CCNGG 4 cut(s) 468, 657, 804, 896
StyI CCWWGG 4 cut(s) 501, 585, 766, 791
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 1 cut(s) 786
TaqI TCGA 2 cut(s) 462, 780
TasI AATT 3 cut(s) 310, 332, 453
TatI WGTACW 1 cut(s) 161
TauI GCSGC 1 cut(s) 750
TfiI GAWTC 3 cut(s) 208, 238, 449
Tru1I TTAA 4 cut(s) 231, 279, 719, 1074
Tru9I TTAA 4 cut(s) 231, 279, 719, 1074
TscAI CASTG 4 cut(s) 304, 525, 604, 779
TseFI GTSAC 5 cut(s) 94, 599, 770, 980, 1067
TseI GCWGC 3 cut(s) 343, 832, 908
Tsp45I GTSAC 5 cut(s) 94, 599, 770, 980, 1067
TspDTI ATGAA 4 cut(s) 69, 326, 641, 702
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 4 cut(s) 304, 525, 604, 779
VpaK11BI GGWCC 3 cut(s) 476, 893, 956
XapI RAATTY 1 cut(s) 332
XceI RCATGY 1 cut(s) 915
XmaJI CCTAGG 1 cut(s) 791
XspI CTAG 3 cut(s) 608, 612, 792
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.