Rh6BG314800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000837
Physical Location & Seq
Forward (+)
54485001 .. 54486665
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG314800.1

Sequence Viewer

Length: 321 bp
ATGGTGTCAAACTTCAGATCCCAGTCATTGATCTCAAAGGCATTGGGAATGATCAGTCTAGAGGAAATGGTCAATGCACTTAAAGATGCATGTGAAACATGGGGTTTCTTTCAAATAGTTAACCATGGAGTTCCACTTGCTGCTATGGAGGAGATGCTTGATAGTATTCGTAGATTCCATGAGCAGGCCCCAGAAGACAAGATGGAGCTGTACTCTCGTGACTTTAAGAAGCAGGTCAACTTTAATGGCAGTGGAGATCCGAAATTGTGTGCAAAATCAGGAGCAGATTGGAGAGACACTATTCTGCAGAGCCGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

106

Amino Acids

12.1

Weight (kDa)

6.09

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 21 - 101 3.8e-17 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AclWI GGATC 2 cut(s) 12, 251
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 184
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 2 cut(s) 12, 251
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 187
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 1 cut(s) 296
BclI TGATCA 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 189
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 2 cut(s) 76, 144
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 201
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BsaJI CCNNGG 1 cut(s) 124
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 288
BsnI GGCC 1 cut(s) 188
Bsp143I GATC 4 cut(s) 17, 30, 51, 256
Bsp19I CCATGG 1 cut(s) 124
BspANI GGCC 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 2 cut(s) 12, 251
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 4 cut(s) 17, 30, 51, 256
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 124
Bst2BI CACGAG 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 124
BstKTI GATC 4 cut(s) 20, 33, 54, 259
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 17, 30, 51, 256
BstNSI RCATGY 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 17, 256
BstYI RGATCY 2 cut(s) 17, 256
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 211
CviAII CATG 4 cut(s) 90, 99, 125, 179
CviJI RGCY 3 cut(s) 188, 208, 312
CviKI_1 RGCY 3 cut(s) 188, 208, 312
CviQI GTAC 1 cut(s) 211
DpnI GATC 4 cut(s) 19, 32, 53, 258
DpnII GATC 4 cut(s) 17, 30, 51, 256
Eco130I CCWWGG 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 4 cut(s) 93, 102, 128, 182
FaiI YATR 5 cut(s) 91, 100, 126, 146, 180
FatI CATG 4 cut(s) 89, 98, 124, 178
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 59
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 188
Hin1II CATG 4 cut(s) 93, 102, 128, 182
HincII GTYRAC 2 cut(s) 121, 238
HindII GTYRAC 2 cut(s) 121, 238
HinfI GANTC 1 cut(s) 174
HpaI GTTAAC 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 121, 238
Hpy188I TCNGA 2 cut(s) 17, 261
Hpy188III TCNNGA 3 cut(s) 59, 218, 279
Hpy8I GTNNAC 2 cut(s) 121, 238
Hpy99I CGWCG 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 77, 89, 272, 307
Hsp92II CATG 4 cut(s) 93, 102, 128, 182
Ksp22I TGATCA 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 4 cut(s) 17, 30, 51, 256
LmnI GCTCC 2 cut(s) 205, 281
LpnPI CCDG 5 cut(s) 35, 170, 204, 218, 264
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 2 cut(s) 76, 144
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 218
MalI GATC 4 cut(s) 19, 32, 53, 258
MboI GATC 4 cut(s) 17, 30, 51, 256
MboII GAAGA 1 cut(s) 206
MflI RGATCY 2 cut(s) 17, 256
MluCI AATT 1 cut(s) 263
MnlI CCTC 2 cut(s) 55, 142
Mph1103I ATGCAT 1 cut(s) 91
MseI TTAA 4 cut(s) 81, 120, 225, 243
NcoI CCATGG 1 cut(s) 124
NdeII GATC 4 cut(s) 17, 30, 51, 256
NlaIII CATG 4 cut(s) 93, 102, 128, 182
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 91
NspI RCATGY 1 cut(s) 93
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 2 cut(s) 17, 256
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SaqAI TTAA 4 cut(s) 81, 120, 225, 243
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 17, 30, 51, 256
Sau96I GGNCC 1 cut(s) 187
SetI ASST 2 cut(s) 210, 237
SfaNI GCATC 2 cut(s) 76, 144
SfcI CTRYAG 1 cut(s) 305
Sse9I AATT 1 cut(s) 263
SspMI CTAG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 124
TaqI TCGA 1 cut(s) 316
TasI AATT 1 cut(s) 263
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 4 cut(s) 81, 120, 225, 243
Tru9I TTAA 4 cut(s) 81, 120, 225, 243
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 218
TspRI CASTG 1 cut(s) 256
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 1 cut(s) 93
XspI CTAG 1 cut(s) 59
Zsp2I ATGCAT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.