RLG00000012386

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000837
Physical Location & Seq
Reverse (-)
18172443 .. 18173856
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012386

Sequence Viewer

Length: 1104 bp
ATGGCAGATGCAGACTACATGGTAGAAAAAGAAAAAGAACTGTATGCGTTTGATGACCTTAAAATAGGTGTCAAAGGGCTCTCAGACTCCGGCGTGGCCAAGAATATTCCCAGAAACTTCATCCAGCCACCGGAGACTGTAAAGAACACAGCACCAGATGATGGTGTCAAACTTCAAATCCCAACTGTTGATCTCAGATGCATTGGCAATGATCACGCTCGAGAAGGAATAGTGAAGGCAATTAAAGATGCATGTGAAACATGGGGATTCTTTCAGATAGTTAACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGTATTCGTAGATTTCATGAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATTCGATCAACTTCTATTGCAGTGGAGATTTGAAAGTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCCTGCAGAGCCGTAGATGATCAATGGGACTTTGAAGCACTGCCTCAAGTGTGCAGACGTGAAATAAGTGAGTACATCAAAAACTTGATTGAACTTCAGAATGTGCTATCCGAATTACTATTGGAAGCATTAGGGCTTAGCAAGGATCACCTAGCACTCATGGAGTGCTTGAAAGCTTTCTCACTATCATGCCACTATTATCCAATTTGCCCTGAGCCTGAACTGACTCTAGGCACAATCAAACACTCAGATCCCTCATTTCTTACCTTAGTCCTGCAAGACAAGATTGGAGGGCTCCAAGTTCTCCATAAGGATCAATGGGTTGATGTACCGCCAGTAGATGGAGCATTAGTAGCAAATCTAGGTGACCTTATGCAGCTCGTTACTAATAACAAGTTCAAGAGTGTGGAACATAGTGTGCTAGCTACCCCGCTTGTCAAGCCACGAATATCAGTTGCATCATTCTTTGCTCCTATGGATAGCGACAAACGTGTCAAGCCGTATGGGCCTATAAAGGAGCTTCTATCTGAGAACAATCCCCCAATATACAGGGACACCCTTTATGGAGAATATACTGGGTATTACAGAGTTAATGGACAAAATGGCAGCTCTGCCCTGCCTAATTTCAGACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

368

Amino Acids

41.32

Weight (kDa)

5.38

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 60 - 160 1e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 218 - 313 7.9e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 956
AccB7I CCANNNNNTGG 2 cut(s) 161, 806
AciI CCGC 2 cut(s) 797, 896
AclWI GGATC 3 cut(s) 618, 710, 786
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 391
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 3 cut(s) 374, 539, 795
AfiI CCNNNNNNNGG 3 cut(s) 130, 161, 806
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 8 cut(s) 176, 323, 388, 424, 500, 557, 637, 865
AjiI CACGTC 1 cut(s) 524
AluBI AGCT 5 cut(s) 641, 844, 890, 985, 1074
AluI AGCT 5 cut(s) 641, 844, 890, 985, 1074
Alw26I GTCTC 2 cut(s) 128, 450
AlwI GGATC 3 cut(s) 618, 710, 786
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 2 cut(s) 96, 971
ApeKI GCWGC 4 cut(s) 346, 443, 841, 1071
ApoI RAATTY 1 cut(s) 391
Asp700I GAANNNNTTC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 971
AsuHPI GGTGA 2 cut(s) 605, 842
AsuNHI GCTAGC 1 cut(s) 886
AvaI CYCGRG 1 cut(s) 219
BalI TGGCCA 1 cut(s) 98
BanII GRGCYC 2 cut(s) 81, 762
BauI CACGAG 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 363
BbvI GCAGC 4 cut(s) 358, 455, 853, 1083
BccI CCATC 3 cut(s) 155, 358, 800
BceAI ACGGC 2 cut(s) 461, 949
BclI TGATCA 2 cut(s) 211, 484
BcoDI GTCTC 2 cut(s) 128, 450
BfaI CTAG 4 cut(s) 617, 695, 827, 887
BfmI CTRYAG 2 cut(s) 351, 469
BglI GCCNNNNNGGC 1 cut(s) 970
BisI GCNGC 4 cut(s) 347, 444, 842, 1072
BlpI GCTNAGC 1 cut(s) 602
BlsI GCNGC 4 cut(s) 348, 445, 843, 1073
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 971
BmiI GGNNCC 1 cut(s) 761
BmrI ACTGGG 1 cut(s) 1050
BmsI GCATC 4 cut(s) 188, 238, 306, 932
BmtI GCTAGC 1 cut(s) 890
BmuI ACTGGG 1 cut(s) 1050
BpiI GAAGAC 1 cut(s) 363
BplI GAGNNNNNCTC 2 cut(s) 359, 391
Bpu10I CCTNAGC 1 cut(s) 678
Bpu1102I GCTNAGC 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 161, 806
Bse1I ACTGG 2 cut(s) 800, 1045
Bse3DI GCAATG 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 130, 161, 806
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 5 cut(s) 96, 208, 669, 726, 984
BseNI ACTGG 2 cut(s) 800, 1045
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 4 cut(s) 358, 455, 853, 1083
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 2 cut(s) 98, 973
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 90, 131
BslFI GGGAC 2 cut(s) 506, 1031
BslI CCNNNNNNNGG 3 cut(s) 130, 161, 806
BsmAI GTCTC 2 cut(s) 128, 450
BsmFI GGGAC 2 cut(s) 506, 1031
BsnI GGCC 2 cut(s) 98, 973
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 81, 762
Bsp143I GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
Bsp1720I GCTNAGC 1 cut(s) 602
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 2 cut(s) 797, 896
BspANI GGCC 2 cut(s) 98, 973
BspCNI CTCAG 5 cut(s) 95, 207, 670, 725, 985
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 761
BspMAI CTGCAG 2 cut(s) 355, 473
BspOI GCTAGC 1 cut(s) 890
BspPI GGATC 3 cut(s) 618, 710, 786
BsrDI GCAATG 1 cut(s) 214
BsrI ACTGG 2 cut(s) 800, 1045
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 378
Bst4CI ACNGT 3 cut(s) 42, 139, 187
BstC8I GCNNGC 3 cut(s) 351, 432, 888
BstDEI CTNAG 7 cut(s) 82, 194, 602, 678, 712, 733, 993
BstDSI CCRYGG 1 cut(s) 286
BstEII GGTNACC 1 cut(s) 830
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 7 cut(s) 193, 214, 399, 487, 613, 718, 781
BstMAI GTCTC 2 cut(s) 128, 450
BstMBI GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
BstMWI GCNNNNNNNGC 4 cut(s) 440, 818, 904, 970
BstNSI RCATGY 1 cut(s) 255
BstPI GGTNACC 1 cut(s) 830
BstSFI CTRYAG 2 cut(s) 351, 469
BstV1I GCAGC 4 cut(s) 358, 455, 853, 1083
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 2 cut(s) 98, 973
BtgI CCRYGG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 524
BtsCI GGATG 1 cut(s) 120
BtsI GCAGTG 2 cut(s) 418, 503
BtsIMutI CAGTG 2 cut(s) 418, 503
Cac8I GCNNGC 3 cut(s) 351, 432, 888
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 971
Csp6I GTAC 3 cut(s) 373, 538, 794
CviAII CATG 7 cut(s) 19, 252, 261, 287, 341, 625, 654
CviQI GTAC 3 cut(s) 373, 538, 794
DdeI CTNAG 7 cut(s) 82, 194, 602, 678, 712, 733, 993
DpnI GATC 7 cut(s) 192, 213, 398, 486, 612, 717, 780
DpnII GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
DrdI GACNNNNNNGTC 1 cut(s) 956
DseDI GACNNNNNNGTC 1 cut(s) 956
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 286
Eco24I GRGCYC 2 cut(s) 81, 762
Eco57I CTGAAG 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 830
EcoRI GAATTC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 286
EcoT22I ATGCAT 2 cut(s) 203, 253
EcoT38I GRGCYC 2 cut(s) 81, 762
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 7 cut(s) 22, 255, 264, 290, 344, 628, 657
FaqI GGGAC 2 cut(s) 506, 1031
FatI CATG 7 cut(s) 18, 251, 260, 286, 340, 624, 653
FauI CCCGC 1 cut(s) 903
FbaI TGATCA 2 cut(s) 211, 484
Fnu4HI GCNGC 4 cut(s) 347, 444, 842, 1072
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 2 cut(s) 81, 762
Fsp4HI GCNGC 4 cut(s) 347, 444, 842, 1072
FspBI CTAG 4 cut(s) 617, 695, 827, 887
GluI GCNGC 4 cut(s) 347, 444, 842, 1072
HaeIII GGCC 2 cut(s) 98, 973
HapII CCGG 2 cut(s) 90, 131
Hin1II CATG 7 cut(s) 22, 255, 264, 290, 344, 628, 657
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 3 cut(s) 86, 267, 691
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 90, 131
HphI GGTGA 2 cut(s) 605, 842
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 8 cut(s) 85, 197, 276, 564, 577, 715, 994, 1094
Hpy188III TCNNGA 5 cut(s) 221, 341, 380, 388, 865
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 2 cut(s) 218, 229
HpyCH4III ACNGT 3 cut(s) 42, 139, 187
HpyCH4IV ACGT 2 cut(s) 523, 955
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 818, 904, 970
HpyF3I CTNAG 7 cut(s) 82, 194, 602, 678, 712, 733, 993
HpySE526I ACGT 2 cut(s) 523, 955
Hsp92II CATG 7 cut(s) 22, 255, 264, 290, 344, 628, 657
Ksp22I TGATCA 2 cut(s) 211, 484
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
LmnI GCTCC 4 cut(s) 765, 809, 940, 982
Lsp1109I GCAGC 4 cut(s) 358, 455, 853, 1083
LweI GCATC 4 cut(s) 188, 238, 306, 932
MaeI CTAG 4 cut(s) 617, 695, 827, 887
MaeII ACGT 2 cut(s) 523, 955
MaeIII GTNAC 3 cut(s) 380, 830, 847
MalI GATC 7 cut(s) 192, 213, 398, 486, 612, 717, 780
MboI GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
MboII GAAGA 1 cut(s) 368
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 81, 762
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 5 cut(s) 240, 391, 578, 669, 1087
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 80, 685
MnlI CCTC 4 cut(s) 304, 519, 730, 749
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 203, 253
MroXI GAANNNNTTC 1 cut(s) 641
MscI TGGCCA 1 cut(s) 98
MseI TTAA 4 cut(s) 60, 243, 282, 1056
MslI CAYNNNNRTG 1 cut(s) 652
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 2 cut(s) 90, 131
MwoI GCNNNNNNNGC 4 cut(s) 440, 818, 904, 970
NcoI CCATGG 1 cut(s) 286
NdeII GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
NheI GCTAGC 1 cut(s) 886
NlaIII CATG 7 cut(s) 22, 255, 264, 290, 344, 628, 657
NlaIV GGNNCC 1 cut(s) 761
NmuCI GTSAC 2 cut(s) 380, 830
NsiI ATGCAT 2 cut(s) 203, 253
NspI RCATGY 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 219
PagI TCATGA 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 267
PflMI CCANNNNNTGG 2 cut(s) 161, 806
PkrI GCNGC 4 cut(s) 348, 445, 843, 1073
PleI GAGTC 2 cut(s) 80, 685
PpsI GAGTC 2 cut(s) 80, 685
PspEI GGTNACC 1 cut(s) 830
PspN4I GGNNCC 1 cut(s) 761
PspPI GGNCC 1 cut(s) 971
PstI CTGCAG 2 cut(s) 355, 473
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 3 cut(s) 374, 539, 795
RsaNI GTAC 3 cut(s) 373, 538, 794
RseI CAYNNNNRTG 1 cut(s) 652
SaqAI TTAA 4 cut(s) 60, 243, 282, 1056
SatI GCNGC 4 cut(s) 347, 444, 842, 1072
Sau3AI GATC 7 cut(s) 190, 211, 396, 484, 610, 715, 778
Sau96I GGNCC 1 cut(s) 971
SchI GAGTC 2 cut(s) 80, 685
SduI GDGCHC 2 cut(s) 81, 762
SfaNI GCATC 4 cut(s) 188, 238, 306, 932
SfcI CTRYAG 2 cut(s) 351, 469
Sfr274I CTCGAG 1 cut(s) 219
SlaI CTCGAG 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 652
SmlI CTYRAG 2 cut(s) 219, 510
SmoI CTYRAG 2 cut(s) 219, 510
Sse9I AATT 5 cut(s) 240, 391, 578, 669, 1087
SsiI CCGC 2 cut(s) 797, 896
SspI AATATT 1 cut(s) 106
SspMI CTAG 4 cut(s) 617, 695, 827, 887
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 42, 139, 187
TaiI ACGT 2 cut(s) 526, 958
TaqI TCGA 2 cut(s) 220, 395
TasI AATT 5 cut(s) 240, 391, 578, 669, 1087
TatI WGTACW 2 cut(s) 372, 537
TfiI GAWTC 1 cut(s) 267
Tru1I TTAA 4 cut(s) 60, 243, 282, 1056
Tru9I TTAA 4 cut(s) 60, 243, 282, 1056
TscAI CASTG 2 cut(s) 418, 510
TseFI GTSAC 2 cut(s) 380, 830
TseI GCWGC 4 cut(s) 346, 443, 841, 1071
Tsp45I GTSAC 2 cut(s) 380, 830
TspDTI ATGAA 2 cut(s) 109, 329
TspRI CASTG 2 cut(s) 418, 510
Van91I CCANNNNNTGG 2 cut(s) 161, 806
XapI RAATTY 1 cut(s) 391
XceI RCATGY 1 cut(s) 255
XhoI CTCGAG 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 4 cut(s) 617, 695, 827, 887
Zsp2I ATGCAT 2 cut(s) 203, 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.