RLG00000012370

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000837
Physical Location & Seq
Reverse (-)
17989539 .. 17990134
596 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012370

Sequence Viewer

Length: 480 bp
ATGGCAGATGAAGACTGCACTGCAGAAAAAGAAAAAGAATTGTGTGCGTTTGATGACCTCAAAATAGGTGTCAAAGGTCTTGCAGACTCCGGGGTGACCAGGATTCCCAGAATCTTCATCCACCCACCAGAGACTGTAAAGTACACAACACCAGAAAATGGTGTCAAACTTCAGATCCCAGTCATTGATCTCAAAGGCATTGGGAATGATCACAGTCTAGAGGAAATGGTCAATGCACTTAAAGATGCATGTGAAACATGGGGGTTCTTTCAAATAGTTAACCATGGAGTTCCACTTGCTGCTATGGAGGAGATGCTTGATAGTATTCGTAGATTCCATGAGCAGGCCCCAGAAGACAAGATGGAGCTCTACTCTCGTGACTTTAAGAAGCAGGTCAACTTTAATGGCAGTGGAGATCCGAAATTGTGTGCAAAATCAGGAGCAGATTGGAGAGACACTATTCTGCAGAGCCGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

160

Amino Acids

17.91

Weight (kDa)

5.13

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 154 1.2e-20 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 158
AclWI GGATC 2 cut(s) 169, 410
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 158, 343
AgsI TTSAA 1 cut(s) 272
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 1 cut(s) 367
AluI AGCT 1 cut(s) 367
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 125, 447
AlwI GGATC 2 cut(s) 169, 410
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 299
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AspS9I GGNCC 1 cut(s) 346
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 106
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 2 cut(s) 18, 360
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 355
BceAI ACGGC 1 cut(s) 456
BcgI CGANNNNNNTGC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 100
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 2 cut(s) 125, 447
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 2 cut(s) 21, 464
BfuAI ACCTGC 1 cut(s) 382
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 2 cut(s) 91, 100
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 91, 100
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 235, 303
BmuI ACTGGG 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 18, 360
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpuMI CCSGG 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 90, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 343
Bse1I ACTGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 90, 283
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 158, 343
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 1 cut(s) 286
BshFI GGCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 158, 343
BsmAI GTCTC 2 cut(s) 125, 447
BsnI GGCC 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 4 cut(s) 174, 187, 208, 415
Bsp19I CCATGG 1 cut(s) 283
BspANI GGCC 1 cut(s) 347
BspLI GGNNCC 1 cut(s) 348
BspMAI CTGCAG 2 cut(s) 25, 468
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 2 cut(s) 169, 410
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 90, 283
BssMI GATC 4 cut(s) 174, 187, 208, 415
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 283
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 100
Bst4CI ACNGT 2 cut(s) 136, 215
BstC8I GCNNGC 1 cut(s) 345
BstDSI CCRYGG 1 cut(s) 283
BstEII GGTNACC 1 cut(s) 94
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 4 cut(s) 177, 190, 211, 418
BstMAI GTCTC 2 cut(s) 125, 447
BstMBI GATC 4 cut(s) 174, 187, 208, 415
BstNI CCWGG 1 cut(s) 100
BstNSI RCATGY 1 cut(s) 252
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 89, 98
BstSFI CTRYAG 2 cut(s) 21, 464
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 2 cut(s) 18, 360
BstX2I RGATCY 2 cut(s) 174, 415
BstYI RGATCY 2 cut(s) 174, 415
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 2 cut(s) 18, 415
BtsIMutI CAGTG 2 cut(s) 18, 415
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 1 cut(s) 142
CviAII CATG 4 cut(s) 249, 258, 284, 338
CviJI RGCY 3 cut(s) 347, 367, 471
CviKI_1 RGCY 3 cut(s) 347, 367, 471
CviQI GTAC 1 cut(s) 142
DpnI GATC 4 cut(s) 176, 189, 210, 417
DpnII GATC 4 cut(s) 174, 187, 208, 415
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 155
Eco91I GGTNACC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 4 cut(s) 252, 261, 287, 341
FaiI YATR 5 cut(s) 250, 259, 285, 305, 339
FatI CATG 4 cut(s) 248, 257, 283, 337
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 1 cut(s) 218
GluI GCNGC 1 cut(s) 300
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 90
Hin1II CATG 4 cut(s) 252, 261, 287, 341
HincII GTYRAC 2 cut(s) 280, 397
HindII GTYRAC 2 cut(s) 280, 397
HinfI GANTC 4 cut(s) 86, 103, 111, 333
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 144, 280, 397
Hpy188I TCNGA 2 cut(s) 174, 420
Hpy188III TCNNGA 3 cut(s) 218, 377, 438
Hpy8I GTNNAC 3 cut(s) 144, 280, 397
Hpy99I CGWCG 1 cut(s) 477
HpyCH4III ACNGT 2 cut(s) 136, 215
HpyCH4V TGCA 7 cut(s) 18, 23, 83, 236, 248, 431, 466
Hsp92II CATG 4 cut(s) 252, 261, 287, 341
Ksp22I TGATCA 1 cut(s) 208
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 4 cut(s) 174, 187, 208, 415
LmnI GCTCC 2 cut(s) 364, 440
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 2 cut(s) 235, 303
MaeI CTAG 1 cut(s) 218
MaeIII GTNAC 2 cut(s) 94, 377
MalI GATC 4 cut(s) 176, 189, 210, 417
MboI GATC 4 cut(s) 174, 187, 208, 415
MboII GAAGA 3 cut(s) 23, 106, 365
MflI RGATCY 2 cut(s) 174, 415
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 2 cut(s) 38, 422
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 3 cut(s) 68, 214, 301
Mph1103I ATGCAT 1 cut(s) 250
MseI TTAA 4 cut(s) 240, 279, 384, 402
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 91, 100
MvaI CCWGG 1 cut(s) 100
NciI CCSGG 1 cut(s) 91
NcoI CCATGG 1 cut(s) 283
NdeII GATC 4 cut(s) 174, 187, 208, 415
NlaIII CATG 4 cut(s) 252, 261, 287, 341
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 2 cut(s) 94, 377
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 1 cut(s) 252
PfeI GAWTC 3 cut(s) 103, 111, 333
PflMI CCANNNNNTGG 1 cut(s) 158
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 98
PspEI GGTNACC 1 cut(s) 94
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 1 cut(s) 346
PstI CTGCAG 2 cut(s) 25, 468
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 2 cut(s) 174, 415
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SacI GAGCTC 1 cut(s) 369
SaqAI TTAA 4 cut(s) 240, 279, 384, 402
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 4 cut(s) 174, 187, 208, 415
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 91, 100
SduI GDGCHC 1 cut(s) 369
SetI ASST 5 cut(s) 60, 70, 79, 369, 396
SfaNI GCATC 2 cut(s) 235, 303
SfcI CTRYAG 2 cut(s) 21, 464
Sse9I AATT 2 cut(s) 38, 422
SspMI CTAG 1 cut(s) 218
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 89, 98
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 136, 215
TaqI TCGA 1 cut(s) 475
TasI AATT 2 cut(s) 38, 422
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 3 cut(s) 103, 111, 333
Tru1I TTAA 4 cut(s) 240, 279, 384, 402
Tru9I TTAA 4 cut(s) 240, 279, 384, 402
TscAI CASTG 2 cut(s) 25, 415
TseFI GTSAC 2 cut(s) 94, 377
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 2 cut(s) 94, 377
TspDTI ATGAA 2 cut(s) 24, 106
TspRI CASTG 2 cut(s) 25, 415
Van91I CCANNNNNTGG 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 252
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.