MD05G1205100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000837
Physical Location & Seq
Forward (+)
33451505 .. 33455153
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1205100.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGCAAACGGACTGTCGAGTTATGACATAGCCAAGGATATGAAGGATTTTGAGGAAACAAAAGCTGGAGTAAAAGGACTTGTGGACACAGGGCTGACCAAACTCCCCAGGATGTTCATCCAGCCAGCAGACAGACTTTCAGATTTGGCAATAAAAAATATAGAATTACGAGTTCCTGTCATAGATTTTGAGGGCTTTGATCAAGATTTCCGGCGTCCGCAGATTGTGAAGGAGGTTCGTAAAGCATGCGAAACGTGGGGGTTCTTTCAAATGGTTAACCATGGAGTTCCGGAGAAGGCGATGGATAAGGCGTTAGAAGGGTCTCGACGATTTCATGAGCAGCCGAAGGAGGACAGGATGGACTGGTATTCTCGTGACTATAATAAAAAAGTGGCATACTACAGCAGTGTAAAACCGTTGGCAACCAAAGCAGCAGTTTGGAGAGACTCGGTAGGGTTTGATTTCCTGGACGGTCCATTGGATCCTGAGAACATTCCATCGGTTTGCAGAGAGGCGGTGCAGGAGTACACCAAACACATGAAGAACCTGGGAAATGTACTGTCCGGTTTGCTATCGGAGGCCTTGGGGCTTAGCGCTGCCTACCTGGAACGCAGAGAGTTCCTGAAGGCTGAAAGTCTGGTGCTCCATTACTCCCCGAGTTGTCCAGAACCCAAGCTGACCCTCAACACTTCCAGGCATACAGATCCTTCCTCGTTTACCGTAGTCTTACAAGACAAAACTGGCGGCCTGCAAGTCCTCCACGAAGGTCATTGGGTTGATGTTCCTCCAACTAAAGGAACTCTCGTGGCCAATGTTGGTGATCTTCTGCAATTTATCTCCAACGGCAAGTTCAAAAGCGTAGTGCATAGGGTACTGGCAGGAACAGCGGGGCCTAGAGTATCAGCTGTATGTTTTTTCTATCCAAGTGCAACCGAGAGAATCAAACCCATTGGACCAATCAAGGAGATTCTTTCTGGAGACCAGCCTATTTACAGGGAAGTAAGCGTTCGTTCGTATCTTGCTTACTACAGAGCAAATGGATTCGAGGGTAAATCAGTGGTTCCAGATTTCGAATTAGCCCCTACGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.65

Weight (kDa)

7.09

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 155 1.1e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 306 7.8e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 289
AciI CCGC 4 cut(s) 218, 515, 744, 887
AclWI GGATC 3 cut(s) 476, 489, 698
AcoI YGGCCR 1 cut(s) 807
AcuI CTGAAG 1 cut(s) 644
AcyI GRCGYC 1 cut(s) 214
AfaI GTAC 3 cut(s) 527, 558, 873
AfeI AGCGCT 1 cut(s) 595
AfiI CCNNNNNNNGG 1 cut(s) 794
AgsI TTSAA 2 cut(s) 269, 853
AjnI CCWGG 5 cut(s) 107, 465, 546, 603, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 3 cut(s) 65, 676, 905
AluI AGCT 3 cut(s) 65, 676, 905
Alw21I GWGCWC 1 cut(s) 645
Alw26I GTCTC 3 cut(s) 327, 438, 972
AlwI GGATC 3 cut(s) 476, 489, 698
Ama87I CYCGRG 1 cut(s) 655
Aor13HI TCCGGA 1 cut(s) 289
Aor51HI AGCGCT 1 cut(s) 595
AoxI GGCC 4 cut(s) 579, 745, 807, 890
ApeKI GCWGC 3 cut(s) 340, 431, 596
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 3 cut(s) 473, 890, 953
AsuHPI GGTGA 1 cut(s) 830
AsuII TTCGAA 1 cut(s) 1071
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 2 cut(s) 473, 953
BalI TGGCCA 1 cut(s) 809
BamHI GGATCC 1 cut(s) 481
BauI CACGAG 2 cut(s) 372, 803
Bbv12I GWGCWC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 352, 443, 583
BccI CCATC 3 cut(s) 295, 352, 505
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 5 cut(s) 109, 467, 548, 605, 694
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 327, 438, 972
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 2 cut(s) 400, 1027
BfoI RGCGCY 1 cut(s) 597
BisI GCNGC 4 cut(s) 341, 432, 597, 745
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 4 cut(s) 342, 433, 598, 746
Bme1390I CCNGG 5 cut(s) 109, 467, 548, 605, 694
Bme18I GGWCC 2 cut(s) 473, 953
BmeT110I CYCGRG 1 cut(s) 655
BmgT120I GGNCC 3 cut(s) 473, 890, 953
BmiI GGNNCC 3 cut(s) 483, 891, 1062
BmrFI CCNGG 5 cut(s) 109, 467, 548, 605, 694
BpmI CTGGAG 2 cut(s) 87, 996
Bpu1102I GCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 1071
BsaBI GATNNNNATC 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 214
BsaI GGTCTC 2 cut(s) 327, 972
BsaJI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BsaWI WCCGGW 2 cut(s) 289, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse1I ACTGG 3 cut(s) 368, 745, 879
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 289
BseBI CCWGG 5 cut(s) 109, 467, 548, 605, 694
BseDI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BseGI GGATG 3 cut(s) 117, 117, 363
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 3 cut(s) 368, 745, 879
BseXI GCAGC 3 cut(s) 352, 443, 583
BsgI GTGCAG 1 cut(s) 539
BshFI GGCC 4 cut(s) 581, 747, 809, 892
BsiHKAI GWGCWC 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 3 cut(s) 211, 290, 564
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 3 cut(s) 327, 438, 972
BsnI GGCC 4 cut(s) 581, 747, 809, 892
Bso31I GGTCTC 2 cut(s) 327, 972
BsoBI CYCGRG 1 cut(s) 655
Bsp119I TTCGAA 1 cut(s) 1071
Bsp1286I GDGCHC 1 cut(s) 645
Bsp13I TCCGGA 1 cut(s) 289
Bsp143I GATC 4 cut(s) 199, 481, 703, 820
Bsp1720I GCTNAGC 1 cut(s) 590
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 4 cut(s) 218, 515, 744, 887
BspANI GGCC 4 cut(s) 581, 747, 809, 892
BspCNI CTCAG 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 289
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 3 cut(s) 483, 891, 1062
BspPI GGATC 3 cut(s) 476, 489, 698
BspT104I TTCGAA 1 cut(s) 1071
BspTNI GGTCTC 2 cut(s) 327, 972
BsrI ACTGG 3 cut(s) 368, 745, 879
BssECI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BssMI GATC 4 cut(s) 199, 481, 703, 820
BssNI GRCGYC 1 cut(s) 214
BssSI CACGAG 2 cut(s) 372, 803
BssT1I CCWWGG 3 cut(s) 33, 280, 582
Bst2BI CACGAG 2 cut(s) 372, 803
Bst2UI CCWGG 5 cut(s) 109, 467, 548, 605, 694
Bst4CI ACNGT 6 cut(s) 15, 417, 473, 561, 721, 1087
BstACI GRCGYC 1 cut(s) 214
BstBI TTCGAA 1 cut(s) 1071
BstC8I GCNNGC 3 cut(s) 126, 247, 749
BstDEI CTNAG 2 cut(s) 486, 590
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 3 cut(s) 117, 117, 363
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 4 cut(s) 202, 484, 706, 823
BstMAI GTCTC 3 cut(s) 327, 438, 972
BstMBI GATC 4 cut(s) 199, 481, 703, 820
BstMWI GCNNNNNNNGC 2 cut(s) 428, 884
BstNI CCWGG 5 cut(s) 109, 467, 548, 605, 694
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 5 cut(s) 107, 465, 546, 603, 692
BstSFI CTRYAG 2 cut(s) 400, 1027
BstV1I GCAGC 3 cut(s) 352, 443, 583
BstX2I RGATCY 2 cut(s) 481, 703
BstYI RGATCY 2 cut(s) 481, 703
BsuRI GGCC 4 cut(s) 581, 747, 809, 892
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 3 cut(s) 117, 117, 363
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 412, 1062
Cac8I GCNNGC 3 cut(s) 126, 247, 749
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 3 cut(s) 473, 890, 953
CseI GACGC 1 cut(s) 203
Csp6I GTAC 3 cut(s) 526, 557, 872
CviAII CATG 4 cut(s) 246, 281, 335, 538
CviQI GTAC 3 cut(s) 526, 557, 872
DdeI CTNAG 2 cut(s) 486, 590
DpnI GATC 4 cut(s) 201, 483, 705, 822
DpnII GATC 4 cut(s) 199, 481, 703, 820
EaeI YGGCCR 1 cut(s) 807
Eco130I CCWWGG 3 cut(s) 33, 280, 582
Eco147I AGGCCT 1 cut(s) 581
Eco31I GGTCTC 2 cut(s) 327, 972
Eco47I GGWCC 2 cut(s) 473, 953
Eco47III AGCGCT 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 644
Eco88I CYCGRG 1 cut(s) 655
EcoO109I RGGNCCY 1 cut(s) 890
EcoRII CCWGG 5 cut(s) 107, 465, 546, 603, 692
EcoT14I CCWWGG 3 cut(s) 33, 280, 582
ErhI CCWWGG 3 cut(s) 33, 280, 582
FaeI CATG 4 cut(s) 249, 284, 338, 541
FatI CATG 4 cut(s) 245, 280, 334, 537
FauI CCCGC 1 cut(s) 880
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 4 cut(s) 341, 432, 597, 745
FokI GGATG 3 cut(s) 104, 124, 370
Fsp4HI GCNGC 4 cut(s) 341, 432, 597, 745
FspBI CTAG 1 cut(s) 894
GlaI GCGC 1 cut(s) 595
GluI GCNGC 4 cut(s) 341, 432, 597, 745
GsuI CTGGAG 2 cut(s) 87, 996
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 4 cut(s) 581, 747, 809, 892
HapII CCGG 3 cut(s) 211, 290, 564
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 4 cut(s) 249, 284, 338, 541
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 4 cut(s) 446, 939, 967, 1041
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 3 cut(s) 211, 290, 564
HphI GGTGA 1 cut(s) 830
Hpy166II GTNNAC 4 cut(s) 85, 277, 528, 717
Hpy188I TCNGA 2 cut(s) 142, 577
Hpy8I GTNNAC 4 cut(s) 85, 277, 528, 717
Hpy99I CGWCG 1 cut(s) 330
HpyAV CCTTC 8 cut(s) 37, 223, 289, 311, 340, 619, 717, 758
HpyCH4III ACNGT 6 cut(s) 15, 417, 473, 561, 721, 1087
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 6 cut(s) 507, 520, 751, 829, 865, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 884
HpyF3I CTNAG 2 cut(s) 486, 590
HpySE526I ACGT 1 cut(s) 254
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 4 cut(s) 249, 284, 338, 541
HspAI GCGC 1 cut(s) 594
Kpn2I TCCGGA 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 4 cut(s) 199, 481, 703, 820
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 3 cut(s) 352, 443, 583
MaeI CTAG 1 cut(s) 894
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 1 cut(s) 374
MalI GATC 4 cut(s) 201, 483, 705, 822
MboI GATC 4 cut(s) 199, 481, 703, 820
MboII GAAGA 2 cut(s) 553, 815
MflI RGATCY 2 cut(s) 481, 703
MhlI GDGCHC 1 cut(s) 645
MlsI TGGCCA 1 cut(s) 809
MluCI AATT 3 cut(s) 164, 830, 1073
MluNI TGGCCA 1 cut(s) 809
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 812, 864
Mox20I TGGCCA 1 cut(s) 809
MroI TCCGGA 1 cut(s) 289
MscI TGGCCA 1 cut(s) 809
MseI TTAA 2 cut(s) 276, 1090
Msp20I TGGCCA 1 cut(s) 809
MspA1I CMGCKG 2 cut(s) 887, 905
MspI CCGG 3 cut(s) 211, 290, 564
MspR9I CCNGG 5 cut(s) 109, 467, 548, 605, 694
MvaI CCWGG 5 cut(s) 109, 467, 548, 605, 694
MwoI GCNNNNNNNGC 2 cut(s) 428, 884
NcoI CCATGG 1 cut(s) 280
NdeII GATC 4 cut(s) 199, 481, 703, 820
NlaIII CATG 4 cut(s) 249, 284, 338, 541
NlaIV GGNNCC 3 cut(s) 483, 891, 1062
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 1 cut(s) 249
NspV TTCGAA 1 cut(s) 1071
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 1 cut(s) 581
PfeI GAWTC 3 cut(s) 939, 967, 1041
PfoI TCCNGGA 1 cut(s) 465
PkrI GCNGC 4 cut(s) 342, 433, 598, 746
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp6I CCWGG 5 cut(s) 107, 465, 546, 603, 692
PspGI CCWGG 5 cut(s) 107, 465, 546, 603, 692
PspN4I GGNNCC 3 cut(s) 483, 891, 1062
PspPI GGNCC 3 cut(s) 473, 890, 953
PsuI RGATCY 2 cut(s) 481, 703
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 3 cut(s) 527, 558, 873
RsaNI GTAC 3 cut(s) 526, 557, 872
SaqAI TTAA 2 cut(s) 276, 1090
SatI GCNGC 4 cut(s) 341, 432, 597, 745
Sau3AI GATC 4 cut(s) 199, 481, 703, 820
Sau96I GGNCC 3 cut(s) 473, 890, 953
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 5 cut(s) 109, 467, 548, 605, 694
SduI GDGCHC 1 cut(s) 645
SetI ASST 8 cut(s) 67, 237, 257, 549, 606, 678, 769, 907
SfcI CTRYAG 2 cut(s) 400, 1027
SfuI TTCGAA 1 cut(s) 1071
SinI GGWCC 2 cut(s) 473, 953
SphI GCATGC 1 cut(s) 249
Sse9I AATT 3 cut(s) 164, 830, 1073
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 4 cut(s) 218, 515, 744, 887
SspMI CTAG 1 cut(s) 894
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 5 cut(s) 107, 465, 546, 603, 692
StyI CCWWGG 3 cut(s) 33, 280, 582
TaaI ACNGT 6 cut(s) 15, 417, 473, 561, 721, 1087
TaiI ACGT 1 cut(s) 257
TaqI TCGA 4 cut(s) 17, 325, 1044, 1071
TasI AATT 3 cut(s) 164, 830, 1073
TatI WGTACW 2 cut(s) 525, 556
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 3 cut(s) 939, 967, 1041
Tru1I TTAA 2 cut(s) 276, 1090
Tru9I TTAA 2 cut(s) 276, 1090
TscAI CASTG 2 cut(s) 412, 1062
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 3 cut(s) 340, 431, 596
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 56, 106, 323, 554
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 412, 1062
VpaK11BI GGWCC 2 cut(s) 473, 953
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.