MD10G1186700.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000837
Physical Location & Seq
Forward (+)
28095661 .. 28097111
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1186700.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCAGCTCGGGATTACAATGCCGAAAAGGAGTTGTACGCGTTCGATGACCTGAAAATCGGCGTCAAAGGGCTCGCGGACTCTGGGGTGACCAAGATTCCCAGGATCTTCTTCCACCCACCGGAGAGCCCGAAGAACTCTCAGCCAGAAAATGGCGTTCACCTTCGAATCCCGATAGTTAACCATGGCGTTCCACTTGCTGTAATGGAGGAGATGCTGGAAGGTATAAGAAAATTTCACGGGCAGCCCCCCGAGGCCAAGAGGGAGTTCGTTAGGGCTCACCGCAGATCACCTAGCGCAGATGGGTGCTTGAAATCTTTCTCGTTATCATGCCACTACTACCCTGTTTGCCCGGAGCCCGATTTGACTCTTGGCACAATCAAACACTCAGACCCCTCCTTTCTCACCTTACTCCTGCAAGATAAGATTGGGGGCCTCCAAGTTCTCCATAAAAACCAATGGGTCGATGTGCCGCCAGTGGATGGAACTTTACTCGTTACTAATGACAAGTTCAAGAGTGTGGAGCACAGAGTACTCGCCACTCCGCTTGTCAAGCCTCGAATATCAGTTTCATCTTTTCTCGTTCCTAGTGCCAAGGACAAACTCAACCCCTACAAGCCAATCAAGGAGCTTCTGTCTGAAACCAATCCACCCAAATACAGAGATACGCTTCACGGAGAATATACTGCGTATTACAGAGTCAATGGACAAAATGGTGGCTCTGCCCTGCCTCATTTTCAACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

250

Amino Acids

27.87

Weight (kDa)

8.84

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 109 - 195 9.8e-23 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 152, 482
AccII CGCG 2 cut(s) 41, 77
AciI CCGC 4 cut(s) 77, 283, 473, 545
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 38, 534
AfiI CCNNNNNNNGG 3 cut(s) 121, 152, 482
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 3 cut(s) 313, 514, 740
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 2 cut(s) 8, 631
AluI AGCT 2 cut(s) 8, 631
Alw21I GWGCWC 1 cut(s) 528
AlwI GGATC 1 cut(s) 113
Ama87I CYCGRG 2 cut(s) 9, 251
AoxI GGCC 2 cut(s) 255, 433
ApeKI GCWGC 2 cut(s) 5, 244
ApoI RAATTY 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 317
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 433
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 5 cut(s) 100, 152, 272, 282, 397
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 2 cut(s) 9, 251
BanII GRGCYC 4 cut(s) 75, 131, 280, 360
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 17, 256
BccI CCATC 2 cut(s) 296, 476
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 353
BfaI CTAG 2 cut(s) 294, 588
BisI GCNGC 3 cut(s) 6, 245, 473
BlsI GCNGC 3 cut(s) 7, 246, 474
BmcAI AGTACT 1 cut(s) 534
Bme1390I CCNGG 2 cut(s) 103, 353
BmeT110I CYCGRG 2 cut(s) 9, 251
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 2 cut(s) 357, 434
BmrFI CCNGG 2 cut(s) 103, 353
BmsI GCATC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 353
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 101, 184, 252, 594
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 152, 482
Bse1I ACTGG 1 cut(s) 476
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 4 cut(s) 101, 184, 252, 594
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 3 cut(s) 121, 152, 482
BseMII CTCAG 2 cut(s) 155, 402
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 17, 256
Bsh1236I CGCG 2 cut(s) 41, 77
BshFI GGCC 2 cut(s) 257, 435
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 9, 251
BsiSI CCGG 2 cut(s) 122, 353
BslI CCNNNNNNNGG 3 cut(s) 121, 152, 482
BsnI GGCC 2 cut(s) 257, 435
BsoBI CYCGRG 2 cut(s) 9, 251
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 5 cut(s) 75, 131, 280, 360, 528
Bsp143I GATC 2 cut(s) 105, 287
Bsp19I CCATGG 1 cut(s) 184
BspACI CCGC 4 cut(s) 77, 283, 473, 545
BspANI GGCC 2 cut(s) 257, 435
BspCNI CTCAG 2 cut(s) 154, 401
BspFNI CGCG 2 cut(s) 41, 77
BspLI GGNNCC 2 cut(s) 357, 434
BspPI GGATC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 166
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 4 cut(s) 101, 184, 252, 594
BssMI GATC 2 cut(s) 105, 287
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 184, 594
Bst2UI CCWGG 1 cut(s) 103
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 141, 388
BstDSI CCRYGG 1 cut(s) 184
BstEII GGTNACC 1 cut(s) 88
BstF5I GGATG 1 cut(s) 487
BstFNI CGCG 2 cut(s) 41, 77
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 108, 290
BstMBI GATC 2 cut(s) 105, 287
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 1 cut(s) 103
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 101, 351
BstUI CGCG 2 cut(s) 41, 77
BstV1I GCAGC 2 cut(s) 17, 256
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 2 cut(s) 257, 435
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 1 cut(s) 75
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 37, 533
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 2 cut(s) 185, 330
CviQI GTAC 2 cut(s) 37, 533
DdeI CTNAG 2 cut(s) 141, 388
DpnI GATC 2 cut(s) 107, 289
DpnII GATC 2 cut(s) 105, 287
Eco130I CCWWGG 2 cut(s) 184, 594
Eco24I GRGCYC 4 cut(s) 75, 131, 280, 360
Eco88I CYCGRG 2 cut(s) 9, 251
Eco91I GGTNACC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 433
EcoO65I GGTNACC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 2 cut(s) 184, 594
EcoT38I GRGCYC 4 cut(s) 75, 131, 280, 360
ErhI CCWWGG 2 cut(s) 184, 594
FaeI CATG 2 cut(s) 188, 333
FaiI YATR 5 cut(s) 186, 227, 331, 450, 684
FatI CATG 2 cut(s) 184, 329
Fnu4HI GCNGC 3 cut(s) 6, 245, 473
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 4 cut(s) 75, 131, 280, 360
Fsp4HI GCNGC 3 cut(s) 6, 245, 473
FspBI CTAG 2 cut(s) 294, 588
GlaI GCGC 1 cut(s) 298
GluI GCNGC 3 cut(s) 6, 245, 473
HaeIII GGCC 2 cut(s) 257, 435
HapII CCGG 2 cut(s) 122, 353
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 2 cut(s) 188, 333
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 5 cut(s) 80, 97, 168, 367, 699
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 2 cut(s) 122, 353
HphI GGTGA 5 cut(s) 100, 152, 272, 282, 397
Hpy166II GTNNAC 2 cut(s) 160, 181
Hpy188I TCNGA 2 cut(s) 391, 640
Hpy188III TCNNGA 3 cut(s) 11, 172, 514
Hpy8I GTNNAC 2 cut(s) 160, 181
HpyAV CCTTC 2 cut(s) 173, 215
HpyCH4V TGCA 2 cut(s) 418, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 2 cut(s) 141, 388
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 2 cut(s) 188, 333
HspAI GCGC 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 105, 287
LmnI GCTCC 3 cut(s) 355, 523, 628
Lsp1109I GCAGC 2 cut(s) 17, 256
LweI GCATC 1 cut(s) 204
MaeI CTAG 2 cut(s) 294, 588
MaeIII GTNAC 2 cut(s) 88, 496
MalI GATC 2 cut(s) 107, 289
MboI GATC 2 cut(s) 105, 287
MboII GAAGA 3 cut(s) 100, 103, 145
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 5 cut(s) 75, 131, 280, 360, 528
MluCI AATT 1 cut(s) 233
MluI ACGCGT 1 cut(s) 39
MlyI GAGTC 3 cut(s) 74, 361, 708
MnlI CCTC 7 cut(s) 202, 247, 255, 406, 446, 567, 741
MroXI GAANNNNTTC 1 cut(s) 317
MseI TTAA 1 cut(s) 180
MspI CCGG 2 cut(s) 122, 353
MspR9I CCNGG 2 cut(s) 103, 353
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 2 cut(s) 41, 77
MwoI GCNNNNNNNGC 1 cut(s) 553
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 1 cut(s) 184
NdeII GATC 2 cut(s) 105, 287
NlaIII CATG 2 cut(s) 188, 333
NlaIV GGNNCC 2 cut(s) 357, 434
NmuCI GTSAC 1 cut(s) 88
NspV TTCGAA 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 317
PfeI GAWTC 2 cut(s) 97, 168
PflMI CCANNNNNTGG 2 cut(s) 152, 482
PkrI GCNGC 3 cut(s) 7, 246, 474
PleI GAGTC 3 cut(s) 74, 361, 707
PpsI GAGTC 3 cut(s) 74, 361, 707
Psp6I CCWGG 1 cut(s) 101
PspEI GGTNACC 1 cut(s) 88
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 357, 434
PspPI GGNCC 1 cut(s) 433
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 2 cut(s) 38, 534
RsaNI GTAC 2 cut(s) 37, 533
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 3 cut(s) 6, 245, 473
Sau3AI GATC 2 cut(s) 105, 287
Sau96I GGNCC 1 cut(s) 433
ScaI AGTACT 1 cut(s) 534
SchI GAGTC 3 cut(s) 74, 361, 708
ScrFI CCNGG 2 cut(s) 103, 353
SduI GDGCHC 5 cut(s) 75, 131, 280, 360, 528
SetI ASST 7 cut(s) 10, 54, 165, 226, 295, 410, 633
SfaNI GCATC 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 166
Sse9I AATT 1 cut(s) 233
SsiI CCGC 4 cut(s) 77, 283, 473, 545
SspMI CTAG 2 cut(s) 294, 588
StyD4I CCNGG 2 cut(s) 101, 351
StyI CCWWGG 2 cut(s) 184, 594
TaqI TCGA 4 cut(s) 45, 166, 465, 559
TasI AATT 1 cut(s) 233
TatI WGTACW 1 cut(s) 532
TauI GCSGC 1 cut(s) 475
TfiI GAWTC 2 cut(s) 97, 168
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 483
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 5, 244
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 1 cut(s) 561
TspGWI ACGGA 1 cut(s) 690
TspRI CASTG 1 cut(s) 483
Van91I CCANNNNNTGG 2 cut(s) 152, 482
XapI RAATTY 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 317
XspI CTAG 2 cut(s) 294, 588
ZrmI AGTACT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.