Rh6BG315300

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000837
Physical Location & Seq
Reverse (-)
54501238 .. 54502936
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG315300.1

Sequence Viewer

Length: 864 bp
ATGGCAGCAGTTGTGGATTGTCATCAGCATGAGAAGGAGCTCAAAACATTCGATGAAAGCAAAGCTGGCGTCAAAGGACTTGCAGACGCTGGGGTGACCAAGCTCCCAATCATGTTCATACACCCTCCAGAGAATCTACTAAACTCATCATTCCCGGAAGATGATGAGCAATATCACAAAATTCGCAACCTTCAATTTCCGGTGGTTGATCTCAGTGGTTTTGATAACAGCGAGAGGCGAAAAGAAATCGTCAATGACATAAACAAAGCAGCTGAGTCATGGGGCTTCTTTCAGTTGGTGAACCATGGAATTCCGCTTGGTGTTATGGAGGGGATCCAGAAAAGTATCCGAGGGTTTCATGAGCTGCCCCAGGAGGAGAAGGTGAAGTGGTACTCGCGCGATTTTACGCAGAAGGTTAACTTCTTCAGCTATGGAGACTTGAAGGTGTCGACGCCAGCTGACTGGAGGGATACCTTGTCATGCAAGGTCCTAGAAGACCCGGAAAGCTTTGAAGCAATACCGCAAGTGTGCAGGCGAGAAGTAAGTGAGTATATGAAACACATCGATGGAGTGCAAGAGAAGTTATCCGAGTTATTTTCAGAAGCTTTAGGTCTTAGCAGTGATAATCTTGCAAGCGCAGGGTGCTTCAAATCGAGGTCATTGGCGTGCCACTATTTCCCGATTTGCCCTGAACCGCATTTGACTATGGGCGGAACCAAGCATTCGGACCTCGGCTTTCTCACCTTGCTTCTTCAAGACAGTGTTGGCGGCCTACAAGTTCTTCATCAAAATGTTTGGATTGATGTTCCCCCAGTGAAGGGAGCTTTGCTAGCAAATATTGGTGACATGATGCAGGTGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

287

Amino Acids

32.19

Weight (kDa)

5.29

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 67 - 166 1.2e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 220 - 286 2.2e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccI GTMKAC 1 cut(s) 449
AccII CGCG 2 cut(s) 397, 399
AciI CCGC 5 cut(s) 314, 521, 695, 711, 768
AclWI GGATC 2 cut(s) 328, 341
AcsI RAATTY 2 cut(s) 180, 309
AcuI CTGAAG 1 cut(s) 409
AcyI GRCGYC 2 cut(s) 69, 452
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 817, 818
AgsI TTSAA 5 cut(s) 194, 442, 512, 649, 755
AjnI CCWGG 1 cut(s) 369
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 328, 341
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 3 cut(s) 5, 269, 364
ApoI RAATTY 2 cut(s) 180, 309
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AspLEI GCGC 2 cut(s) 399, 638
AspS9I GGNCC 2 cut(s) 487, 727
AsuC2I CCSGG 2 cut(s) 155, 500
AsuHPI GGTGA 5 cut(s) 106, 310, 394, 733, 854
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 2 cut(s) 487, 727
BamHI GGATCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 3 cut(s) 17, 281, 351
BccI CCATC 1 cut(s) 560
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 2 cut(s) 356, 463
BcnI CCSGG 2 cut(s) 155, 500
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 2 cut(s) 491, 830
BfuAI ACCTGC 1 cut(s) 844
BfuI GTATCC 2 cut(s) 356, 463
BisI GCNGC 4 cut(s) 6, 270, 365, 769
BlsI GCNGC 4 cut(s) 7, 271, 366, 770
Bme1390I CCNGG 3 cut(s) 155, 371, 500
Bme18I GGWCC 2 cut(s) 487, 727
BmgT120I GGNCC 2 cut(s) 487, 727
BmiI GGNNCC 2 cut(s) 335, 715
BmrFI CCNGG 3 cut(s) 155, 371, 500
BmrI ACTGGG 1 cut(s) 806
BmsI GCATC 1 cut(s) 840
BmtI GCTAGC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 2 cut(s) 111, 484
BpuMI CCSGG 2 cut(s) 155, 500
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 69, 452
BsaJI CCNNGG 4 cut(s) 304, 349, 369, 730
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 2 cut(s) 817, 818
Bse1I ACTGG 2 cut(s) 467, 812
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 4 cut(s) 304, 349, 369, 730
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 817, 818
BseMII CTCAG 2 cut(s) 226, 264
BseNI ACTGG 2 cut(s) 467, 812
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 3 cut(s) 17, 281, 351
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 2 cut(s) 397, 399
BshFI GGCC 1 cut(s) 771
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 3 cut(s) 155, 200, 500
BslI CCNNNNNNNGG 2 cut(s) 817, 818
BsmAI GTCTC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 1 cut(s) 771
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 208, 333
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 5 cut(s) 314, 521, 695, 711, 768
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 2 cut(s) 225, 265
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 2 cut(s) 397, 399
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 335, 715
BspMI ACCTGC 1 cut(s) 844
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 2 cut(s) 328, 341
BsrI ACTGG 2 cut(s) 467, 812
BssECI CCNNGG 4 cut(s) 304, 349, 369, 730
BssMI GATC 2 cut(s) 208, 333
BssNI GRCGYC 2 cut(s) 69, 452
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 761
BstACI GRCGYC 2 cut(s) 69, 452
BstC8I GCNNGC 6 cut(s) 67, 456, 533, 634, 667, 831
BstDEI CTNAG 3 cut(s) 212, 273, 614
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 94
BstFNI CGCG 2 cut(s) 397, 399
BstHHI GCGC 2 cut(s) 399, 638
BstKTI GATC 2 cut(s) 211, 336
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 2 cut(s) 208, 333
BstMWI GCNNNNNNNGC 3 cut(s) 66, 642, 830
BstNI CCWGG 1 cut(s) 371
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 3 cut(s) 153, 369, 498
BstUI CGCG 2 cut(s) 397, 399
BstV1I GCAGC 3 cut(s) 17, 281, 351
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 462
BstYI RGATCY 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 2 cut(s) 356, 463
BsuRI GGCC 1 cut(s) 771
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 4 cut(s) 220, 625, 766, 819
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 6 cut(s) 67, 456, 533, 634, 667, 831
CaiI CAGNNNCTG 1 cut(s) 89
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 399, 638
Cfr13I GGNCC 2 cut(s) 487, 727
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 3 cut(s) 58, 95, 460
Csp6I GTAC 1 cut(s) 391
CviAII CATG 7 cut(s) 29, 112, 279, 305, 359, 480, 847
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 212, 273, 614
DpnI GATC 2 cut(s) 210, 335
DpnII GATC 2 cut(s) 208, 333
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 487, 727
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 409
Eco91I GGTNACC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 487
EcoO65I GGTNACC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 7 cut(s) 32, 115, 282, 308, 362, 483, 850
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FatI CATG 7 cut(s) 28, 111, 278, 304, 358, 479, 846
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 4 cut(s) 6, 270, 365, 769
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 6, 270, 365, 769
FspBI CTAG 2 cut(s) 491, 830
GlaI GCGC 2 cut(s) 398, 637
GluI GCNGC 4 cut(s) 6, 270, 365, 769
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 2 cut(s) 111, 484
HaeIII GGCC 1 cut(s) 771
HapII CCGG 3 cut(s) 155, 200, 500
HgaI GACGC 3 cut(s) 58, 95, 460
HhaI GCGC 2 cut(s) 399, 638
Hin1I GRCGYC 2 cut(s) 69, 452
Hin1II CATG 7 cut(s) 32, 115, 282, 308, 362, 483, 850
Hin6I GCGC 2 cut(s) 397, 636
HinP1I GCGC 2 cut(s) 397, 636
HincII GTYRAC 2 cut(s) 418, 450
HindII GTYRAC 2 cut(s) 418, 450
HindIII AAGCTT 2 cut(s) 505, 603
HinfI GANTC 2 cut(s) 133, 275
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 3 cut(s) 155, 200, 500
HphI GGTGA 5 cut(s) 106, 310, 394, 733, 854
Hpy166II GTNNAC 3 cut(s) 301, 418, 450
Hpy188I TCNGA 4 cut(s) 350, 589, 601, 727
Hpy188III TCNNGA 5 cut(s) 128, 337, 359, 679, 755
Hpy8I GTNNAC 3 cut(s) 301, 418, 450
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 6 cut(s) 28, 200, 373, 406, 436, 811
HpyCH4III ACNGT 1 cut(s) 761
HpyCH4V TGCA 6 cut(s) 83, 483, 531, 574, 632, 853
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 642, 830
HpyF3I CTNAG 3 cut(s) 212, 273, 614
Hsp92I GRCGYC 2 cut(s) 69, 452
Hsp92II CATG 7 cut(s) 32, 115, 282, 308, 362, 483, 850
HspAI GCGC 2 cut(s) 397, 636
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 208, 333
LmnI GCTCC 3 cut(s) 37, 108, 821
Lsp1109I GCAGC 3 cut(s) 17, 281, 351
LweI GCATC 1 cut(s) 840
MaeI CTAG 2 cut(s) 491, 830
MaeIII GTNAC 2 cut(s) 94, 842
MalI GATC 2 cut(s) 210, 335
MboI GATC 2 cut(s) 208, 333
MboII GAAGA 5 cut(s) 170, 415, 506, 743, 773
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 3 cut(s) 180, 194, 309
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 8 cut(s) 135, 228, 322, 344, 367, 459, 648, 740
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
MspA1I CMGCKG 2 cut(s) 272, 458
MspI CCGG 3 cut(s) 155, 200, 500
MspR9I CCNGG 3 cut(s) 155, 371, 500
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 2 cut(s) 397, 399
MwoI GCNNNNNNNGC 3 cut(s) 66, 642, 830
NciI CCSGG 2 cut(s) 155, 500
NcoI CCATGG 1 cut(s) 304
NdeII GATC 2 cut(s) 208, 333
NheI GCTAGC 1 cut(s) 829
NlaIII CATG 7 cut(s) 32, 115, 282, 308, 362, 483, 850
NlaIV GGNNCC 2 cut(s) 335, 715
NmeAIII GCCGAG 1 cut(s) 711
NmuCI GTSAC 2 cut(s) 94, 842
PagI TCATGA 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 844
PctI GAATGC 1 cut(s) 721
PfeI GAWTC 1 cut(s) 133
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 4 cut(s) 7, 271, 366, 770
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 369
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 335, 715
PspPI GGNCC 2 cut(s) 487, 727
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 1 cut(s) 333
PvuII CAGCTG 2 cut(s) 272, 458
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
SacI GAGCTC 1 cut(s) 42
SalI GTCGAC 1 cut(s) 448
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 4 cut(s) 6, 270, 365, 769
Sau3AI GATC 2 cut(s) 208, 333
Sau96I GGNCC 2 cut(s) 487, 727
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 155, 371, 500
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 1 cut(s) 840
SinI GGWCC 2 cut(s) 487, 727
SmiMI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
Sse9I AATT 3 cut(s) 180, 194, 309
SsiI CCGC 5 cut(s) 314, 521, 695, 711, 768
SspI AATATT 1 cut(s) 838
SspMI CTAG 2 cut(s) 491, 830
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 3 cut(s) 153, 369, 498
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 761
TaqI TCGA 4 cut(s) 51, 449, 564, 653
TasI AATT 3 cut(s) 180, 194, 309
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 4 cut(s) 220, 625, 766, 819
TseFI GTSAC 2 cut(s) 94, 842
TseI GCWGC 3 cut(s) 5, 269, 364
Tsp45I GTSAC 2 cut(s) 94, 842
TspDTI ATGAA 5 cut(s) 69, 106, 347, 569, 773
TspRI CASTG 4 cut(s) 220, 625, 766, 819
VpaK11BI GGWCC 2 cut(s) 487, 727
XapI RAATTY 2 cut(s) 180, 309
XmiI GTMKAC 1 cut(s) 449
XspI CTAG 2 cut(s) 491, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.