Rw6G026700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000837
Physical Location & Seq
Forward (+)
50170030 .. 50171404
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026700.1

Sequence Viewer

Length: 1083 bp
ATGGTAGAAAAAGAAAAAGAACTGTATGCGTTTGATGACCTTAAAATAGGTGTCAAAGGGCTCTCAGACTCCGGCGTGGCCAAGATTCCCAGAATCTTCATCCACCCACCGGAGACTGTAAAGAACACAGCACCAGATGATGGTGTCAAACTTCAAATCCCAATTGTTGATCTCAGATGCATTGCCAATGATCACGCTCGAGAAGGAATAGTGAAGGCAATTAAAGATGCATGTGAAACATGGGGGTTCTTTCAGATAGTCAACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGTATTCGTAGATTTCATGAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATTCGATCAATTTCTATTGCAGTGGAGATTTGAAAGTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCCTGCAGAGCCTTAGATAATAAATGGGACTTTGAAGCACTGCCTCAAGTGTGCAGACGTGAAATAAGTGAGTACATCAAAAACTTGATTGAACTTCAGAATGTGATATCCGAATTACTGTCGGAAGCATTAGGGCTTAGCAAGGATCACCTAGCACGCATGGGGTGCTTGAAAGCTTTCTCATTATCATGTCACTATTATCCAATTTGCCCCGAGCCTGAACTGACTCTAGGCACAATCAAACACTCAGATCCCTCATTTCTTACCTTAGTCCTGCAAGAAGAGATTGGAGGGCTCCAAGTTCTCCATAAGGATCAATGGGTTGACGTGCCGCCAGTGGATGGAGCATTTGTAGCAAATCTAGGTGACCTTATGCAGCTCGTTACTAATAACAAGTTCAAGAGTGTGGAACATAGAGTGCTAGCTACCCCGCTTGTCAAGCCACGAATATCAGTTGCATCTTTCTTTGCTCCTATGGATAGCGACAAACGTGTCAAGCCGTACGGGCCTATAAAGGAGCTTCTATCTGAGAACAATCCCCCAATATACAGGGACACCCTTTACGGAGAATATACTGGGTATTACAGAGTTAATGGACAAAATGGCAGCTCTGCCCTGCCTAATTTCAGACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

360

Amino Acids

40.65

Weight (kDa)

5.97

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 153 4.1e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 306 1.9e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 935
AccB7I CCANNNNNTGG 2 cut(s) 140, 785
AciI CCGC 2 cut(s) 776, 875
AclWI GGATC 3 cut(s) 597, 689, 765
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 3 cut(s) 353, 518, 947
AfiI CCNNNNNNNGG 3 cut(s) 109, 140, 785
AflIII ACRYGT 1 cut(s) 934
AgsI TTSAA 8 cut(s) 155, 302, 367, 403, 479, 536, 616, 844
AjiI CACGTC 2 cut(s) 503, 772
AluBI AGCT 5 cut(s) 620, 823, 869, 964, 1053
AluI AGCT 5 cut(s) 620, 823, 869, 964, 1053
Alw26I GTCTC 2 cut(s) 107, 429
AlwI GGATC 3 cut(s) 597, 689, 765
Ama87I CYCGRG 2 cut(s) 198, 656
AoxI GGCC 2 cut(s) 78, 950
ApeKI GCWGC 4 cut(s) 325, 422, 820, 1050
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 950
AsuHPI GGTGA 2 cut(s) 584, 821
AsuNHI GCTAGC 1 cut(s) 865
AvaI CYCGRG 2 cut(s) 198, 656
BalI TGGCCA 1 cut(s) 80
BanII GRGCYC 2 cut(s) 63, 741
BauI CACGAG 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 4 cut(s) 337, 434, 832, 1062
BccI CCATC 3 cut(s) 134, 337, 779
BceAI ACGGC 1 cut(s) 928
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 107, 429
BfaI CTAG 4 cut(s) 596, 674, 806, 866
BfmI CTRYAG 2 cut(s) 330, 448
BglI GCCNNNNNGGC 1 cut(s) 949
BisI GCNGC 5 cut(s) 326, 423, 776, 821, 1051
BlpI GCTNAGC 1 cut(s) 581
BlsI GCNGC 5 cut(s) 327, 424, 777, 822, 1052
BmeT110I CYCGRG 2 cut(s) 198, 656
BmgBI CACGTC 2 cut(s) 503, 772
BmgT120I GGNCC 1 cut(s) 950
BmiI GGNNCC 1 cut(s) 740
BmrI ACTGGG 1 cut(s) 1029
BmsI GCATC 4 cut(s) 167, 217, 285, 911
BmtI GCTAGC 1 cut(s) 869
BmuI ACTGGG 1 cut(s) 1029
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu1102I GCTNAGC 1 cut(s) 581
BpuEI CTTGAG 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 140, 785
Bse1I ACTGG 2 cut(s) 779, 1024
Bse3DI GCAATG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 99, 790
BseLI CCNNNNNNNGG 3 cut(s) 109, 140, 785
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 4 cut(s) 78, 187, 705, 963
BseNI ACTGG 2 cut(s) 779, 1024
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 337, 434, 832, 1062
BsgI GTGCAG 1 cut(s) 517
BshFI GGCC 2 cut(s) 80, 952
BsiHKCI CYCGRG 2 cut(s) 198, 656
BsiSI CCGG 2 cut(s) 72, 110
BsiWI CGTACG 1 cut(s) 945
BslFI GGGAC 2 cut(s) 485, 1010
BslI CCNNNNNNNGG 3 cut(s) 109, 140, 785
BsmAI GTCTC 2 cut(s) 107, 429
BsmFI GGGAC 2 cut(s) 485, 1010
BsnI GGCC 2 cut(s) 80, 952
BsoBI CYCGRG 2 cut(s) 198, 656
Bsp1286I GDGCHC 2 cut(s) 63, 741
Bsp143I GATC 6 cut(s) 169, 190, 375, 589, 694, 757
Bsp1720I GCTNAGC 1 cut(s) 581
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 2 cut(s) 776, 875
BspANI GGCC 2 cut(s) 80, 952
BspCNI CTCAG 4 cut(s) 77, 186, 704, 964
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 740
BspMAI CTGCAG 2 cut(s) 334, 452
BspOI GCTAGC 1 cut(s) 869
BspPI GGATC 3 cut(s) 597, 689, 765
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 2 cut(s) 779, 1024
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 6 cut(s) 169, 190, 375, 589, 694, 757
BssSI CACGAG 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 357
Bst4CI ACNGT 3 cut(s) 24, 118, 564
Bst6I CTCTTC 1 cut(s) 720
BstAPI GCANNNNNTGC 1 cut(s) 609
BstC8I GCNNGC 4 cut(s) 330, 411, 601, 867
BstDEI CTNAG 7 cut(s) 64, 173, 457, 581, 691, 712, 972
BstDSI CCRYGG 1 cut(s) 265
BstEII GGTNACC 1 cut(s) 809
BstF5I GGATG 2 cut(s) 99, 790
BstKTI GATC 6 cut(s) 172, 193, 378, 592, 697, 760
BstMAI GTCTC 2 cut(s) 107, 429
BstMBI GATC 6 cut(s) 169, 190, 375, 589, 694, 757
BstMWI GCNNNNNNNGC 5 cut(s) 419, 609, 797, 883, 949
BstNSI RCATGY 1 cut(s) 234
BstPI GGTNACC 1 cut(s) 809
BstSFI CTRYAG 2 cut(s) 330, 448
BstV1I GCAGC 4 cut(s) 337, 434, 832, 1062
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BsuRI GGCC 2 cut(s) 80, 952
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 2 cut(s) 503, 772
BtsCI GGATG 2 cut(s) 99, 790
BtsI GCAGTG 2 cut(s) 397, 482
BtsIMutI CAGTG 3 cut(s) 397, 482, 786
Cac8I GCNNGC 4 cut(s) 330, 411, 601, 867
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 352, 517, 946
CviAII CATG 6 cut(s) 231, 240, 266, 320, 604, 633
CviQI GTAC 3 cut(s) 352, 517, 946
DdeI CTNAG 7 cut(s) 64, 173, 457, 581, 691, 712, 972
DpnI GATC 6 cut(s) 171, 192, 377, 591, 696, 759
DpnII GATC 6 cut(s) 169, 190, 375, 589, 694, 757
DrdI GACNNNNNNGTC 1 cut(s) 935
DseDI GACNNNNNNGTC 1 cut(s) 935
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 63, 741
Eco32I GATATC 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 524
Eco88I CYCGRG 2 cut(s) 198, 656
Eco91I GGTNACC 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 370
EcoRV GATATC 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 265
EcoT22I ATGCAT 2 cut(s) 182, 232
EcoT38I GRGCYC 2 cut(s) 63, 741
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 6 cut(s) 234, 243, 269, 323, 607, 636
FaqI GGGAC 2 cut(s) 485, 1010
FatI CATG 6 cut(s) 230, 239, 265, 319, 603, 632
FauI CCCGC 1 cut(s) 882
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 5 cut(s) 326, 423, 776, 821, 1051
FokI GGATG 2 cut(s) 86, 797
FriOI GRGCYC 2 cut(s) 63, 741
Fsp4HI GCNGC 5 cut(s) 326, 423, 776, 821, 1051
FspBI CTAG 4 cut(s) 596, 674, 806, 866
GluI GCNGC 5 cut(s) 326, 423, 776, 821, 1051
HaeIII GGCC 2 cut(s) 80, 952
HapII CCGG 2 cut(s) 72, 110
Hin1II CATG 6 cut(s) 234, 243, 269, 323, 607, 636
HincII GTYRAC 2 cut(s) 262, 769
HindII GTYRAC 2 cut(s) 262, 769
HindIII AAGCTT 1 cut(s) 618
HinfI GANTC 4 cut(s) 68, 85, 93, 670
HpaII CCGG 2 cut(s) 72, 110
HphI GGTGA 2 cut(s) 584, 821
Hpy166II GTNNAC 2 cut(s) 262, 769
Hpy188I TCNGA 9 cut(s) 67, 176, 255, 543, 556, 568, 694, 973, 1073
Hpy188III TCNNGA 5 cut(s) 200, 320, 359, 367, 844
Hpy8I GTNNAC 2 cut(s) 262, 769
HpyAV CCTTC 2 cut(s) 197, 208
HpyCH4III ACNGT 3 cut(s) 24, 118, 564
HpyCH4IV ACGT 3 cut(s) 502, 771, 934
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 609, 797, 883, 949
HpyF3I CTNAG 7 cut(s) 64, 173, 457, 581, 691, 712, 972
HpySE526I ACGT 3 cut(s) 502, 771, 934
Hsp92II CATG 6 cut(s) 234, 243, 269, 323, 607, 636
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 6 cut(s) 169, 190, 375, 589, 694, 757
LmnI GCTCC 4 cut(s) 744, 788, 919, 961
Lsp1109I GCAGC 4 cut(s) 337, 434, 832, 1062
LweI GCATC 4 cut(s) 167, 217, 285, 911
MaeI CTAG 4 cut(s) 596, 674, 806, 866
MaeII ACGT 3 cut(s) 502, 771, 934
MaeIII GTNAC 4 cut(s) 359, 635, 809, 826
MalI GATC 6 cut(s) 171, 192, 377, 591, 696, 759
MboI GATC 6 cut(s) 169, 190, 375, 589, 694, 757
MboII GAAGA 3 cut(s) 88, 347, 737
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 2 cut(s) 63, 741
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 7 cut(s) 162, 219, 370, 379, 557, 648, 1066
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 2 cut(s) 62, 664
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 4 cut(s) 283, 498, 709, 728
Mox20I TGGCCA 1 cut(s) 80
Mph1103I ATGCAT 2 cut(s) 182, 232
MroXI GAANNNNTTC 1 cut(s) 620
MscI TGGCCA 1 cut(s) 80
MseI TTAA 3 cut(s) 42, 222, 1035
MslI CAYNNNNRTG 1 cut(s) 631
Msp20I TGGCCA 1 cut(s) 80
MspI CCGG 2 cut(s) 72, 110
MunI CAATTG 1 cut(s) 162
MwoI GCNNNNNNNGC 5 cut(s) 419, 609, 797, 883, 949
NcoI CCATGG 1 cut(s) 265
NdeII GATC 6 cut(s) 169, 190, 375, 589, 694, 757
NheI GCTAGC 1 cut(s) 865
NlaIII CATG 6 cut(s) 234, 243, 269, 323, 607, 636
NlaIV GGNNCC 1 cut(s) 740
NmuCI GTSAC 3 cut(s) 359, 635, 809
NsiI ATGCAT 2 cut(s) 182, 232
NspI RCATGY 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 198
PagI TCATGA 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 620
PfeI GAWTC 2 cut(s) 85, 93
Pfl23II CGTACG 1 cut(s) 945
PflMI CCANNNNNTGG 2 cut(s) 140, 785
PkrI GCNGC 5 cut(s) 327, 424, 777, 822, 1052
PleI GAGTC 2 cut(s) 62, 664
PpsI GAGTC 2 cut(s) 62, 664
PspEI GGTNACC 1 cut(s) 809
PspLI CGTACG 1 cut(s) 945
PspN4I GGNNCC 1 cut(s) 740
PspPI GGNCC 1 cut(s) 950
PstI CTGCAG 2 cut(s) 334, 452
PsuI RGATCY 1 cut(s) 694
RsaI GTAC 3 cut(s) 353, 518, 947
RsaNI GTAC 3 cut(s) 352, 517, 946
RseI CAYNNNNRTG 1 cut(s) 631
SaqAI TTAA 3 cut(s) 42, 222, 1035
SatI GCNGC 5 cut(s) 326, 423, 776, 821, 1051
Sau3AI GATC 6 cut(s) 169, 190, 375, 589, 694, 757
Sau96I GGNCC 1 cut(s) 950
SchI GAGTC 2 cut(s) 62, 664
SduI GDGCHC 2 cut(s) 63, 741
SfaNI GCATC 4 cut(s) 167, 217, 285, 911
SfcI CTRYAG 2 cut(s) 330, 448
Sfr274I CTCGAG 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 631
SmlI CTYRAG 2 cut(s) 198, 489
SmoI CTYRAG 2 cut(s) 198, 489
Sse9I AATT 7 cut(s) 162, 219, 370, 379, 557, 648, 1066
SsiI CCGC 2 cut(s) 776, 875
SspMI CTAG 4 cut(s) 596, 674, 806, 866
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 3 cut(s) 24, 118, 564
TaiI ACGT 3 cut(s) 505, 774, 937
TaqI TCGA 2 cut(s) 199, 374
TasI AATT 7 cut(s) 162, 219, 370, 379, 557, 648, 1066
TatI WGTACW 2 cut(s) 351, 516
TauI GCSGC 1 cut(s) 778
TfiI GAWTC 2 cut(s) 85, 93
Tru1I TTAA 3 cut(s) 42, 222, 1035
Tru9I TTAA 3 cut(s) 42, 222, 1035
TscAI CASTG 3 cut(s) 397, 489, 786
TseFI GTSAC 3 cut(s) 359, 635, 809
TseI GCWGC 4 cut(s) 325, 422, 820, 1050
Tsp45I GTSAC 3 cut(s) 359, 635, 809
TspDTI ATGAA 2 cut(s) 88, 308
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 3 cut(s) 397, 489, 786
Van91I CCANNNNNTGG 2 cut(s) 140, 785
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 234
XhoI CTCGAG 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 4 cut(s) 596, 674, 806, 866
Zsp2I ATGCAT 2 cut(s) 182, 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.