Rw6G043250

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000837
Physical Location & Seq
Forward (+)
66630947 .. 66632387
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043250.1

Sequence Viewer

Length: 1065 bp
ATGGCAGTAGTTGTGGATTGTGATCAGCGTGAGAAGGAGCTGAAAGAATTCTACGAAAGCAAAGCTGGCGTCAAAGGACTTGCAGACGCTGGGGTGACCAAGCTCCCAAGCTTGTTCGTACACCCTCCTGAGAATCTACTTAACTCGTTATTCCCACAAGATGATGAGCAATATCAGAAACTTCGCAACCTTCAACTTCCGGTGATTGATCTCAGCGGTTTTGATAACAGCGAGAGGCGAAAAGTAATCATCACAGACATAAACAAAGCAGCTGAGTCATGGGGCTTCTTTCAGTTGGTGAACCATGGAATTCCGCTAGGTCTTATGGAGGGGATCCAGAAAAGTATCCGAGGGTTCCATGAGCTGCCCCAGAAGGAGAAGGTGAAGTGGTACTCGCGCGATTTTACGCAGAAGGTTAGGTTCTTCAGCAATTTTAATTTGAAGGTGTCGACGCCAGCTGCCTGGAGGGATACCTTGTCATGCAAGGTCCTGCAAGACCCAGAAAGCTTTGAAGCAATACCGCAAGTGTGCAGACGAGAAGTAAGTGAGTATATGAAACACATCGATGGAGTGCAAGAGAAGTTATCCGAGTTATTTTCGGAAGCTTTAGGTCTTAGTAGTGACTATCTTGCAAGCATAGGGTGCTTCAAATCGAGGTCATTGGAGTGCCACTATCACCCGATTTGCCCTGAACCCCATTTGACTATGGGTGGAGTCAAGCATTCAGACCTCCCCTTTCTCACCTTGCTCCTTCAAGACGGTGTTGGCGGCCTACAAGTTCTTCATCAAAATGTTTGGATTGATGTTGCCCCCGTGAAGGGAGCTTTGATAGCAAATATTGGTGACATGATGCAGGTTATTACAAATGGCAAGTTCAAGAGCGTGGAGCATAGAGTTTTGATGAAACCAACTGTAGAGCCTCGTATCTCAATTGCTTGTTTTTTAAATACGGATGACTTCCAAAAATCATACGGGCCGATAAAAGAGCTTATATCGGAAAACAATCCACCGATTTATAGCGAAGTCAGATTCGAAGAACAGATAAAAAAATTATATAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

40.21

Weight (kDa)

6.09

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 66 - 166 3.3e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 222 - 316 1.7e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1063
Acc36I ACCTGC 1 cut(s) 844
AccI GTMKAC 1 cut(s) 449
AccII CGCG 2 cut(s) 397, 399
AciI CCGC 4 cut(s) 216, 314, 521, 768
AclWI GGATC 2 cut(s) 328, 341
AcsI RAATTY 2 cut(s) 47, 309
AcuI CTGAAG 1 cut(s) 409
AcyI GRCGYC 2 cut(s) 69, 452
AfaI GTAC 2 cut(s) 120, 392
AfiI CCNNNNNNNGG 2 cut(s) 817, 818
AgsI TTSAA 6 cut(s) 194, 442, 512, 649, 755, 877
AjnI CCWGG 1 cut(s) 461
AlwI GGATC 2 cut(s) 328, 341
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 2 cut(s) 769, 974
ApeKI GCWGC 3 cut(s) 269, 364, 458
ApoI RAATTY 2 cut(s) 47, 309
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 487, 974
AsuHPI GGTGA 7 cut(s) 106, 214, 310, 394, 668, 733, 854
AsuII TTCGAA 1 cut(s) 1032
AvaII GGWCC 1 cut(s) 487
BamHI GGATCC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 281, 351, 445
BccI CCATC 1 cut(s) 560
BciT130I CCWGG 1 cut(s) 463
BciVI GTATCC 2 cut(s) 356, 463
BclI TGATCA 1 cut(s) 22
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 844
BfuI GTATCC 2 cut(s) 356, 463
BisI GCNGC 4 cut(s) 270, 365, 459, 769
BlsI GCNGC 4 cut(s) 271, 366, 460, 770
Bme1390I CCNGG 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 487, 974
BmiI GGNNCC 2 cut(s) 335, 356
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 484
Bpu14I TTCGAA 1 cut(s) 1032
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 69, 452
BsaJI CCNNGG 2 cut(s) 304, 349
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 2 cut(s) 817, 818
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 463
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 304, 349
BseGI GGATG 1 cut(s) 958
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 817, 818
BseMII CTCAG 3 cut(s) 120, 226, 264
BseXI GCAGC 3 cut(s) 281, 351, 445
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 2 cut(s) 397, 399
BshFI GGCC 2 cut(s) 771, 976
BshVI ATCGAT 1 cut(s) 564
BsiSI CCGG 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 817, 818
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 2 cut(s) 771, 976
Bsp119I TTCGAA 1 cut(s) 1032
Bsp143I GATC 3 cut(s) 22, 208, 333
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 4 cut(s) 216, 314, 521, 768
BspANI GGCC 2 cut(s) 771, 976
BspCNI CTCAG 3 cut(s) 121, 225, 265
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 2 cut(s) 397, 399
BspLI GGNNCC 2 cut(s) 335, 356
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 2 cut(s) 328, 341
BspT104I TTCGAA 1 cut(s) 1032
BssECI CCNNGG 2 cut(s) 304, 349
BssMI GATC 3 cut(s) 22, 208, 333
BssNI GRCGYC 2 cut(s) 69, 452
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 761, 913
BstACI GRCGYC 2 cut(s) 69, 452
BstAPI GCANNNNNTGC 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 3 cut(s) 67, 456, 634
BstDEI CTNAG 4 cut(s) 129, 212, 273, 614
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 94
BstF5I GGATG 1 cut(s) 958
BstFNI CGCG 2 cut(s) 397, 399
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 3 cut(s) 25, 211, 336
BstMBI GATC 3 cut(s) 22, 208, 333
BstMWI GCNNNNNNNGC 3 cut(s) 66, 642, 830
BstNI CCWGG 1 cut(s) 463
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 2 cut(s) 397, 399
BstV1I GCAGC 3 cut(s) 281, 351, 445
BstX2I RGATCY 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 462
BstYI RGATCY 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 2 cut(s) 356, 463
BsuRI GGCC 2 cut(s) 771, 976
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 1 cut(s) 958
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 67, 456, 634
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 487, 974
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 3 cut(s) 58, 95, 460
Csp6I GTAC 2 cut(s) 119, 391
CviAII CATG 5 cut(s) 279, 305, 359, 480, 847
CviQI GTAC 2 cut(s) 119, 391
DdeI CTNAG 4 cut(s) 129, 212, 273, 614
DpnI GATC 3 cut(s) 24, 210, 335
DpnII GATC 3 cut(s) 22, 208, 333
DraI TTTAAA 1 cut(s) 945
Eco130I CCWWGG 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 409
Eco91I GGTNACC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 487
EcoO65I GGTNACC 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 47, 309
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 5 cut(s) 282, 308, 362, 483, 850
FatI CATG 5 cut(s) 278, 304, 358, 479, 846
FbaI TGATCA 1 cut(s) 22
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 4 cut(s) 270, 365, 459, 769
FokI GGATG 1 cut(s) 965
Fsp4HI GCNGC 4 cut(s) 270, 365, 459, 769
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 398
GluI GCNGC 4 cut(s) 270, 365, 459, 769
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 2 cut(s) 771, 976
HapII CCGG 1 cut(s) 200
HgaI GACGC 3 cut(s) 58, 95, 460
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 2 cut(s) 69, 452
Hin1II CATG 5 cut(s) 282, 308, 362, 483, 850
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 3 cut(s) 109, 505, 603
HinfI GANTC 4 cut(s) 133, 275, 714, 1029
HpaII CCGG 1 cut(s) 200
HphI GGTGA 7 cut(s) 106, 214, 310, 394, 668, 733, 854
Hpy166II GTNNAC 3 cut(s) 121, 301, 450
Hpy188I TCNGA 7 cut(s) 177, 350, 589, 601, 727, 997, 1028
Hpy188III TCNNGA 4 cut(s) 128, 337, 755, 877
Hpy8I GTNNAC 3 cut(s) 121, 301, 450
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 8 cut(s) 28, 200, 367, 373, 406, 436, 761, 811
HpyCH4III ACNGT 2 cut(s) 761, 913
HpyCH4V TGCA 7 cut(s) 83, 483, 493, 531, 574, 632, 853
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 642, 830
HpyF3I CTNAG 4 cut(s) 129, 212, 273, 614
Hsp92I GRCGYC 2 cut(s) 69, 452
Hsp92II CATG 5 cut(s) 282, 308, 362, 483, 850
HspAI GCGC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 3 cut(s) 22, 208, 333
LmnI GCTCC 5 cut(s) 37, 108, 753, 821, 886
Lsp1109I GCAGC 3 cut(s) 281, 351, 445
LweI GCATC 1 cut(s) 840
MaeI CTAG 1 cut(s) 317
MaeIII GTNAC 3 cut(s) 94, 620, 842
MalI GATC 3 cut(s) 24, 210, 335
MboI GATC 3 cut(s) 22, 208, 333
MboII GAAGA 3 cut(s) 415, 773, 1046
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 1 cut(s) 333
MluCI AATT 7 cut(s) 47, 309, 430, 436, 930, 1049, 1057
MlyI GAGTC 2 cut(s) 284, 723
MnlI CCTC 8 cut(s) 135, 228, 322, 344, 459, 648, 740, 930
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 3 cut(s) 141, 435, 944
MslI CAYNNNNRTG 3 cut(s) 564, 664, 789
MspA1I CMGCKG 3 cut(s) 216, 272, 458
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 463
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 2 cut(s) 397, 399
MwoI GCNNNNNNNGC 3 cut(s) 66, 642, 830
NcoI CCATGG 1 cut(s) 304
NdeII GATC 3 cut(s) 22, 208, 333
NlaIII CATG 5 cut(s) 282, 308, 362, 483, 850
NlaIV GGNNCC 2 cut(s) 335, 356
NmuCI GTSAC 3 cut(s) 94, 620, 842
NspV TTCGAA 1 cut(s) 1032
PctI GAATGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 2 cut(s) 133, 1029
PkrI GCNGC 4 cut(s) 271, 366, 460, 770
PleI GAGTC 2 cut(s) 283, 722
PpsI GAGTC 2 cut(s) 283, 722
PpuMI RGGWCCY 1 cut(s) 487
PsiI TTATAA 1 cut(s) 1063
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 461
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 335, 356
PspPI GGNCC 2 cut(s) 487, 974
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 1 cut(s) 333
PvuII CAGCTG 2 cut(s) 272, 458
RsaI GTAC 2 cut(s) 120, 392
RsaNI GTAC 2 cut(s) 119, 391
RseI CAYNNNNRTG 3 cut(s) 564, 664, 789
SalI GTCGAC 1 cut(s) 448
SaqAI TTAA 3 cut(s) 141, 435, 944
SatI GCNGC 4 cut(s) 270, 365, 459, 769
Sau3AI GATC 3 cut(s) 22, 208, 333
Sau96I GGNCC 2 cut(s) 487, 974
SchI GAGTC 2 cut(s) 284, 723
ScrFI CCNGG 1 cut(s) 463
SfaNI GCATC 1 cut(s) 840
SfcI CTRYAG 1 cut(s) 912
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 1 cut(s) 487
SmiMI CAYNNNNRTG 3 cut(s) 564, 664, 789
Sse9I AATT 7 cut(s) 47, 309, 430, 436, 930, 1049, 1057
SsiI CCGC 4 cut(s) 216, 314, 521, 768
SspI AATATT 1 cut(s) 838
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 761, 913
TaqI TCGA 4 cut(s) 449, 564, 653, 1032
TasI AATT 7 cut(s) 47, 309, 430, 436, 930, 1049, 1057
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 2 cut(s) 133, 1029
Tru1I TTAA 3 cut(s) 141, 435, 944
Tru9I TTAA 3 cut(s) 141, 435, 944
TseFI GTSAC 3 cut(s) 94, 620, 842
TseI GCWGC 3 cut(s) 269, 364, 458
Tsp45I GTSAC 3 cut(s) 94, 620, 842
TspDTI ATGAA 3 cut(s) 569, 773, 917
TspGWI ACGGA 1 cut(s) 965
VpaK11BI GGWCC 1 cut(s) 487
XapI RAATTY 2 cut(s) 47, 309
XmiI GTMKAC 1 cut(s) 449
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.