pycom05g19130

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000837
Physical Location & Seq
Forward (+)
22026458 .. 22027983
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19130.1

Sequence Viewer

Length: 1086 bp
ATGGCAAACGGGCTGTCGAGTTATGACATAACCAAGGATATGAAGGATTTTGAGGAAACAAAAGCTGGAGTAAAAGGACTTGTGGACACAGGGCTGACAAAACTCCCCAGGATGTTCATCCAGCCAGCAGATAGACTTTCAGATTTGGCAATAAAAAATACAGAGTTACGAGTTCCTGTCATAGACTTTGAGGCCTTTGATCAAGATTTCCGACGTCCGCAGATTGTGAAGGAGGTTCGTGAAGCATGCGAAACGTGGGGGTTCTTTCAAATGGTTAACCATGGAGTTCCGGAGAAGGCGATGGATAAGGCGTTAGAAGGGTCTCGACGATTTCATGAGCAGCCGAAGGAGGATAGGATGGACTGGTATTCTCGTGACTATAATAAAAAAGTGGCATACTACAGCAGTGTAAAACCGTTGGCAACGAAAGCAGCAGTTTGGAGAGACTCGGTAGGGTTTGATTTCGCGGACGGTCCATTGGAACCTGAGAACATTCCATCGGTTTGCAGAGATGCGGTGCAGGAGTACACCAAACACATGAAGAACCTGGGAAACGTGCTATCCGGTTTGCTTTCGGAGGCCTTGGGGCTTAGCGCTGACTACCTGGAACGCAGAGAGTTCCTGAAGGTTCAAAGTCTGGTGCTCCATTACTCCCCGAGCTGTCCAGAACCCAAGCTGACCCTCAACACATCCAGGCATACAGATCCTTCCTCTTTTACCGTAGTCTTGCAAGACAAAACTGGCGGCCTGCAAGTCCTCCACGAAGGTCATTGGGTTGATGTTCCTCCAACTAAAGGAACTCTCGTGGCCAATGTCGGTGATCTTCTGCAATTTATCTCCAACGGCAAGTTCAAAAGCGTAGTGCACAGGGTACTGGCAGGAACAGTGGGGCCTAGAGTATCAGCTGTATGTTTTTTCTATCCAACCGAGAGAATCAAACCCATCGGACCAATCAAGGAGATTCTTTCTGGAGACCAGCCTATTTACAGGGAAGTAAGCGTTCGTTCGTATCTTGCTTACTACAGAGCAAATGGATTCGAGGGTAATTCAGTCGTTCCAGATTTCGAATTAGCCCCTACGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.57

Weight (kDa)

6.28

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 155 9e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 306 1.1e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 217
AccII CGCG 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 289
AciI CCGC 4 cut(s) 218, 467, 515, 744
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 807
AcuI CTGAAG 1 cut(s) 644
AcyI GRCGYC 1 cut(s) 214
AfaI GTAC 2 cut(s) 527, 873
AfeI AGCGCT 1 cut(s) 595
AfiI CCNNNNNNNGG 1 cut(s) 794
AgsI TTSAA 3 cut(s) 269, 632, 853
AjnI CCWGG 4 cut(s) 107, 546, 603, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 4 cut(s) 65, 660, 676, 905
AluI AGCT 4 cut(s) 65, 660, 676, 905
Alw21I GWGCWC 2 cut(s) 645, 867
Alw26I GTCTC 3 cut(s) 327, 438, 966
Alw44I GTGCAC 1 cut(s) 863
AlwI GGATC 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 655
Aor13HI TCCGGA 1 cut(s) 289
Aor51HI AGCGCT 1 cut(s) 595
AoxI GGCC 5 cut(s) 192, 579, 745, 807, 890
ApaLI GTGCAC 1 cut(s) 863
ApeKI GCWGC 2 cut(s) 340, 431
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 3 cut(s) 473, 890, 947
AsuHPI GGTGA 1 cut(s) 830
AsuII TTCGAA 1 cut(s) 1065
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 2 cut(s) 473, 947
BaeGI GKGCMC 1 cut(s) 867
BalI TGGCCA 1 cut(s) 809
BauI CACGAG 2 cut(s) 372, 803
Bbv12I GWGCWC 2 cut(s) 645, 867
BbvI GCAGC 2 cut(s) 352, 443
BccI CCATC 4 cut(s) 295, 352, 505, 950
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 4 cut(s) 109, 548, 605, 694
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 327, 438, 966
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 2 cut(s) 400, 1021
BfoI RGCGCY 1 cut(s) 597
BisI GCNGC 3 cut(s) 341, 432, 745
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 3 cut(s) 342, 433, 746
Bme1390I CCNGG 4 cut(s) 109, 548, 605, 694
Bme18I GGWCC 2 cut(s) 473, 947
BmeT110I CYCGRG 1 cut(s) 655
BmgT120I GGNCC 3 cut(s) 473, 890, 947
BmiI GGNNCC 2 cut(s) 483, 891
BmrFI CCNGG 4 cut(s) 109, 548, 605, 694
BmsI GCATC 1 cut(s) 502
BpmI CTGGAG 2 cut(s) 87, 990
Bpu1102I GCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 1065
BsaBI GATNNNNATC 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 214
BsaI GGTCTC 2 cut(s) 327, 966
BsaJI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BsaWI WCCGGW 2 cut(s) 289, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse1I ACTGG 3 cut(s) 368, 745, 879
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 289
BseBI CCWGG 4 cut(s) 109, 548, 605, 694
BseDI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BseGI GGATG 4 cut(s) 117, 117, 363, 689
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 3 cut(s) 368, 745, 879
BseSI GKGCMC 1 cut(s) 867
BseXI GCAGC 2 cut(s) 352, 443
BsgI GTGCAG 1 cut(s) 539
Bsh1236I CGCG 1 cut(s) 467
BshFI GGCC 5 cut(s) 194, 581, 747, 809, 892
BsiHKAI GWGCWC 2 cut(s) 645, 867
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 2 cut(s) 290, 564
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 3 cut(s) 327, 438, 966
BsnI GGCC 5 cut(s) 194, 581, 747, 809, 892
Bso31I GGTCTC 2 cut(s) 327, 966
BsoBI CYCGRG 1 cut(s) 655
Bsp119I TTCGAA 1 cut(s) 1065
Bsp1286I GDGCHC 2 cut(s) 645, 867
Bsp13I TCCGGA 1 cut(s) 289
Bsp143I GATC 3 cut(s) 199, 703, 820
Bsp1720I GCTNAGC 1 cut(s) 590
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 4 cut(s) 218, 467, 515, 744
BspANI GGCC 5 cut(s) 194, 581, 747, 809, 892
BspCNI CTCAG 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 289
BspFNI CGCG 1 cut(s) 467
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 483, 891
BspPI GGATC 1 cut(s) 698
BspT104I TTCGAA 1 cut(s) 1065
BspTNI GGTCTC 2 cut(s) 327, 966
BsrI ACTGG 3 cut(s) 368, 745, 879
BssECI CCNNGG 5 cut(s) 33, 107, 280, 547, 582
BssMI GATC 3 cut(s) 199, 703, 820
BssNI GRCGYC 1 cut(s) 214
BssSI CACGAG 2 cut(s) 372, 803
BssT1I CCWWGG 3 cut(s) 33, 280, 582
Bst2BI CACGAG 2 cut(s) 372, 803
Bst2UI CCWGG 4 cut(s) 109, 548, 605, 694
Bst4CI ACNGT 5 cut(s) 417, 473, 721, 886, 1081
BstACI GRCGYC 1 cut(s) 214
BstBI TTCGAA 1 cut(s) 1065
BstC8I GCNNGC 3 cut(s) 126, 247, 749
BstDEI CTNAG 2 cut(s) 486, 590
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 4 cut(s) 117, 117, 363, 689
BstFNI CGCG 1 cut(s) 467
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 3 cut(s) 202, 706, 823
BstMAI GTCTC 3 cut(s) 327, 438, 966
BstMBI GATC 3 cut(s) 199, 703, 820
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 4 cut(s) 109, 548, 605, 694
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 4 cut(s) 107, 546, 603, 692
BstSFI CTRYAG 2 cut(s) 400, 1021
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 352, 443
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuRI GGCC 5 cut(s) 194, 581, 747, 809, 892
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 4 cut(s) 117, 117, 363, 689
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 412, 891
Cac8I GCNNGC 3 cut(s) 126, 247, 749
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 3 cut(s) 473, 890, 947
Csp6I GTAC 2 cut(s) 526, 872
CviAII CATG 4 cut(s) 246, 281, 335, 538
CviQI GTAC 2 cut(s) 526, 872
DdeI CTNAG 2 cut(s) 486, 590
DpnI GATC 3 cut(s) 201, 705, 822
DpnII GATC 3 cut(s) 199, 703, 820
EaeI YGGCCR 1 cut(s) 807
Eco130I CCWWGG 3 cut(s) 33, 280, 582
Eco147I AGGCCT 2 cut(s) 194, 581
Eco31I GGTCTC 2 cut(s) 327, 966
Eco47I GGWCC 2 cut(s) 473, 947
Eco47III AGCGCT 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 644
Eco88I CYCGRG 1 cut(s) 655
EcoO109I RGGNCCY 1 cut(s) 890
EcoRII CCWGG 4 cut(s) 107, 546, 603, 692
EcoT14I CCWWGG 3 cut(s) 33, 280, 582
ErhI CCWWGG 3 cut(s) 33, 280, 582
FaeI CATG 4 cut(s) 249, 284, 338, 541
FatI CATG 4 cut(s) 245, 280, 334, 537
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 3 cut(s) 341, 432, 745
FokI GGATG 4 cut(s) 104, 124, 370, 676
Fsp4HI GCNGC 3 cut(s) 341, 432, 745
FspBI CTAG 1 cut(s) 894
GlaI GCGC 1 cut(s) 595
GluI GCNGC 3 cut(s) 341, 432, 745
GsuI CTGGAG 2 cut(s) 87, 990
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 5 cut(s) 194, 581, 747, 809, 892
HapII CCGG 2 cut(s) 290, 564
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 4 cut(s) 249, 284, 338, 541
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 4 cut(s) 446, 933, 961, 1035
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 2 cut(s) 290, 564
HphI GGTGA 1 cut(s) 830
Hpy166II GTNNAC 4 cut(s) 85, 277, 528, 865
Hpy188I TCNGA 4 cut(s) 142, 212, 577, 947
Hpy8I GTNNAC 4 cut(s) 85, 277, 528, 865
Hpy99I CGWCG 2 cut(s) 216, 330
HpyAV CCTTC 8 cut(s) 37, 223, 289, 311, 340, 619, 717, 758
HpyCH4III ACNGT 5 cut(s) 417, 473, 721, 886, 1081
HpyCH4IV ACGT 3 cut(s) 214, 254, 555
HpyCH4V TGCA 6 cut(s) 507, 520, 730, 751, 829, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 2 cut(s) 486, 590
HpySE526I ACGT 3 cut(s) 214, 254, 555
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 4 cut(s) 249, 284, 338, 541
HspAI GCGC 1 cut(s) 594
Kpn2I TCCGGA 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 199, 703, 820
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 2 cut(s) 352, 443
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 894
MaeII ACGT 3 cut(s) 214, 254, 555
MaeIII GTNAC 2 cut(s) 165, 374
MalI GATC 3 cut(s) 201, 705, 822
MboI GATC 3 cut(s) 199, 703, 820
MboII GAAGA 2 cut(s) 553, 815
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 2 cut(s) 645, 867
MlsI TGGCCA 1 cut(s) 809
MluCI AATT 3 cut(s) 830, 1045, 1067
MluNI TGGCCA 1 cut(s) 809
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 4 cut(s) 235, 812, 864, 947
Mox20I TGGCCA 1 cut(s) 809
MroI TCCGGA 1 cut(s) 289
MscI TGGCCA 1 cut(s) 809
MseI TTAA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 2 cut(s) 290, 564
MspR9I CCNGG 4 cut(s) 109, 548, 605, 694
MvaI CCWGG 4 cut(s) 109, 548, 605, 694
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 1 cut(s) 428
NcoI CCATGG 1 cut(s) 280
NdeII GATC 3 cut(s) 199, 703, 820
NlaIII CATG 4 cut(s) 249, 284, 338, 541
NlaIV GGNNCC 2 cut(s) 483, 891
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 1 cut(s) 249
NspV TTCGAA 1 cut(s) 1065
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 2 cut(s) 194, 581
PfeI GAWTC 3 cut(s) 933, 961, 1035
PkrI GCNGC 3 cut(s) 342, 433, 746
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp6I CCWGG 4 cut(s) 107, 546, 603, 692
PspGI CCWGG 4 cut(s) 107, 546, 603, 692
PspN4I GGNNCC 2 cut(s) 483, 891
PspPI GGNCC 3 cut(s) 473, 890, 947
PsuI RGATCY 1 cut(s) 703
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 527, 873
RsaNI GTAC 2 cut(s) 526, 872
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 3 cut(s) 341, 432, 745
Sau3AI GATC 3 cut(s) 199, 703, 820
Sau96I GGNCC 3 cut(s) 473, 890, 947
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 4 cut(s) 109, 548, 605, 694
SduI GDGCHC 2 cut(s) 645, 867
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 2 cut(s) 400, 1021
SfuI TTCGAA 1 cut(s) 1065
SinI GGWCC 2 cut(s) 473, 947
SphI GCATGC 1 cut(s) 249
Sse9I AATT 3 cut(s) 830, 1045, 1067
SseBI AGGCCT 2 cut(s) 194, 581
SsiI CCGC 4 cut(s) 218, 467, 515, 744
SspMI CTAG 1 cut(s) 894
StuI AGGCCT 2 cut(s) 194, 581
StyD4I CCNGG 4 cut(s) 107, 546, 603, 692
StyI CCWWGG 3 cut(s) 33, 280, 582
TaaI ACNGT 5 cut(s) 417, 473, 721, 886, 1081
TaiI ACGT 3 cut(s) 217, 257, 558
TaqI TCGA 4 cut(s) 17, 325, 1038, 1065
TasI AATT 3 cut(s) 830, 1045, 1067
TatI WGTACW 1 cut(s) 525
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 3 cut(s) 933, 961, 1035
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 412, 891
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 2 cut(s) 340, 431
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 56, 106, 323, 554
TspRI CASTG 2 cut(s) 412, 891
VneI GTGCAC 1 cut(s) 863
VpaK11BI GGWCC 2 cut(s) 473, 947
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 894
ZraI GACGTC 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.