pycom05g18720

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000837
Physical Location & Seq
Reverse (-)
21565721 .. 21567637
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18720.1

Sequence Viewer

Length: 1035 bp
ATGCCTGGGTTGGATCATGAGCTGGAGGAAGAAATGAAACAATTCGACGAAACAAAAGCTGGTGTGAAAGGCCTTGTAGATTCTGGGGTGACCAAGCTCCCAAGGATGTTCAAACACCCTCCAGAGGATCTACCGTCCCTTAACCATAACAATGGCATTAGCAACCTCCAAGTTCCTGTCATTGATCTCGAAGATGTCGAGGACAGCGAAACGCGGAGCAAAATCATCAATGACCTCAGAAAGGCAGCTGAGGAATGGGGTTTCTTTCAGATTGTGAACCATTCAGTTCCAGTTGGTGTGATGGATGATGTGCTGAAAGGCATTCGACGATTTCATGAGCAGCCCCAGGAAGCCAAGGAGGTGTGGTATTCGCGCGATTTCACGAAGAAGGTCAACTTCGTCAGTAATGCAGAATTCAAGGTGTCTCTTCCAGCTTGTTGGAGAGACACATTGTCTTGCAAGGTCCTAGAAGATGAGCATAACTTCGAGGCAATCCCTGAAATCTGCAGGGCGGAAATAAAAGAGTATATGAGGTACCTTGTTCAATTGAAGGATAAACTTTCCAAATTAATTTCGGAAGCTTTAGGTCTAACCAGCGATTACCTTGACAACCTAAGGTGCTTCGAATCGAGGTCATTGGCATGCCACTATTATCCAGTTTGCCCAGAGCCCCATTTGACTCTCGGCGGACACAAGCACTCTGACCTCGGCTTCATCACCTTGCTCTTGCGAGCTAGTGAAGGCCTACAGTTACTTCATCAAAATGTTTGGATTGACGTTCAACCGGTGGAGGGAGCTTTGCTTGCCAATGTATCTGATATGCTGCAGATCATTACTAATGGCAAGTTCAAGAGTGCAGAGCACAGAGTAGTACTTCCACCAACCCTCGAGCCCCTTACATCAATTGCATGTTTCATTGGCACAGACGACCTCCAAAAACTATACGGGCCGTTAAAGGAGCTTATATCCGAAAACAATCCACCAATTTACAAAGAAGTCGTTTTTGGAGAGTACGTGAAACGTTACAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.21

Weight (kDa)

5.37

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 157 1.6e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 307 1.8e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 534
AccII CGCG 3 cut(s) 214, 373, 375
AciI CCGC 3 cut(s) 214, 512, 687
AclI AACGTT 1 cut(s) 1021
AclWI GGATC 2 cut(s) 21, 135
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 3 cut(s) 536, 873, 1013
AfiI CCNNNNNNNGG 3 cut(s) 124, 241, 791
AgeI ACCGGT 1 cut(s) 784
AgsI TTSAA 6 cut(s) 112, 418, 545, 550, 782, 850
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 4, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
AluBI AGCT 9 cut(s) 22, 59, 97, 248, 434, 581, 734, 797, 961
AluI AGCT 9 cut(s) 22, 59, 97, 248, 434, 581, 734, 797, 961
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 2 cut(s) 429, 438
AlwI GGATC 2 cut(s) 21, 135
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 3 cut(s) 70, 742, 947
ApeKI GCWGC 3 cut(s) 245, 340, 823
ApoI RAATTY 1 cut(s) 413
AseI ATTAAT 1 cut(s) 569
AsiGI ACCGGT 1 cut(s) 784
Asp700I GAANNNNTTC 1 cut(s) 41
Asp718I GGTACC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 463, 947
AsuHPI GGTGA 2 cut(s) 100, 709
AsuII TTCGAA 1 cut(s) 624
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 1 cut(s) 463
AxyI CCTNAGG 1 cut(s) 614
BanI GGYRCC 1 cut(s) 534
BanII GRGCYC 2 cut(s) 672, 894
Bbv12I GWGCWC 1 cut(s) 864
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 3 cut(s) 257, 352, 810
BccI CCATC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 2 cut(s) 6, 347
BcoDI GTCTC 2 cut(s) 429, 438
BfaI CTAG 2 cut(s) 467, 735
BfmI CTRYAG 4 cut(s) 505, 746, 824, 1031
BisI GCNGC 3 cut(s) 246, 341, 824
BlsI GCNGC 3 cut(s) 247, 342, 825
BmcAI AGTACT 1 cut(s) 873
Bme1390I CCNGG 2 cut(s) 6, 347
Bme18I GGWCC 1 cut(s) 463
BmeRI GACNNNNNGTC 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 463, 947
BmiI GGNNCC 1 cut(s) 536
BmrFI CCNGG 2 cut(s) 6, 347
BpmI CTGGAG 2 cut(s) 44, 105
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 1015
BsaJI CCNNGG 5 cut(s) 5, 101, 345, 354, 706
BsaWI WCCGGW 1 cut(s) 784
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 999, 1029
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 241, 791
Bse118I RCCGGY 1 cut(s) 784
Bse1I ACTGG 2 cut(s) 290, 656
Bse21I CCTNAGG 1 cut(s) 614
BseBI CCWGG 2 cut(s) 6, 347
BseDI CCNNGG 5 cut(s) 5, 101, 345, 354, 706
BseGI GGATG 2 cut(s) 111, 310
BseLI CCNNNNNNNGG 3 cut(s) 124, 241, 791
BseMII CTCAG 2 cut(s) 240, 250
BseNI ACTGG 2 cut(s) 290, 656
BseXI GCAGC 3 cut(s) 257, 352, 810
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 3 cut(s) 214, 373, 375
BshFI GGCC 3 cut(s) 72, 744, 949
BshNI GGYRCC 1 cut(s) 534
BshTI ACCGGT 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 1 cut(s) 785
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 3 cut(s) 124, 241, 791
BsmAI GTCTC 2 cut(s) 429, 438
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 3 cut(s) 72, 744, 949
BsoBI CYCGRG 1 cut(s) 887
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 3 cut(s) 672, 864, 894
Bsp143I GATC 4 cut(s) 13, 127, 184, 828
BspACI CCGC 3 cut(s) 214, 512, 687
BspANI GGCC 3 cut(s) 72, 744, 949
BspCNI CTCAG 2 cut(s) 241, 249
BspFNI CGCG 3 cut(s) 214, 373, 375
BspHI TCATGA 2 cut(s) 16, 334
BspLI GGNNCC 1 cut(s) 536
BspMAI CTGCAG 2 cut(s) 509, 828
BspPI GGATC 2 cut(s) 21, 135
BspT104I TTCGAA 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 784
BsrI ACTGG 2 cut(s) 290, 656
BssAI RCCGGY 1 cut(s) 784
BssECI CCNNGG 5 cut(s) 5, 101, 345, 354, 706
BssMI GATC 4 cut(s) 13, 127, 184, 828
BssT1I CCWWGG 2 cut(s) 101, 354
Bst2UI CCWGG 2 cut(s) 6, 347
Bst4CI ACNGT 2 cut(s) 135, 750
Bst6I CTCTTC 1 cut(s) 432
BstBAI YACGTR 1 cut(s) 1015
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 643, 732, 804
BstDEI CTNAG 3 cut(s) 236, 249, 614
BstEII GGTNACC 1 cut(s) 88
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 111, 310
BstFNI CGCG 3 cut(s) 214, 373, 375
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 4 cut(s) 16, 130, 187, 831
BstMAI GTCTC 2 cut(s) 429, 438
BstMBI GATC 4 cut(s) 13, 127, 184, 828
BstMWI GCNNNNNNNGC 1 cut(s) 803
BstNI CCWGG 2 cut(s) 6, 347
BstNSI RCATGY 2 cut(s) 645, 912
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 4, 345
BstSFI CTRYAG 4 cut(s) 505, 746, 824, 1031
BstUI CGCG 3 cut(s) 214, 373, 375
BstV1I GCAGC 3 cut(s) 257, 352, 810
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 2 cut(s) 152, 438
BstYI RGATCY 1 cut(s) 127
Bsu36I CCTNAGG 1 cut(s) 614
BsuRI GGCC 3 cut(s) 72, 744, 949
BtsCI GGATG 2 cut(s) 111, 310
Cac8I GCNNGC 3 cut(s) 643, 732, 804
CciI TCATGA 2 cut(s) 16, 334
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 784
Cfr13I GGNCC 2 cut(s) 463, 947
Csp6I GTAC 3 cut(s) 535, 872, 1012
CspAI ACCGGT 1 cut(s) 784
CviAII CATG 4 cut(s) 17, 335, 642, 909
CviQI GTAC 3 cut(s) 535, 872, 1012
DdeI CTNAG 3 cut(s) 236, 249, 614
DpnI GATC 4 cut(s) 15, 129, 186, 830
DpnII GATC 4 cut(s) 13, 127, 184, 828
DriI GACNNNNNGTC 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 432
EciI GGCGGA 2 cut(s) 527, 702
Eco130I CCWWGG 2 cut(s) 101, 354
Eco147I AGGCCT 2 cut(s) 72, 744
Eco24I GRGCYC 2 cut(s) 672, 894
Eco47I GGWCC 1 cut(s) 463
Eco81I CCTNAGG 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 887
Eco91I GGTNACC 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 4, 345
EcoT14I CCWWGG 2 cut(s) 101, 354
EcoT38I GRGCYC 2 cut(s) 672, 894
ErhI CCWWGG 2 cut(s) 101, 354
FaeI CATG 4 cut(s) 20, 338, 645, 912
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 121
FatI CATG 4 cut(s) 16, 334, 641, 908
Fnu4HI GCNGC 3 cut(s) 246, 341, 824
FokI GGATG 2 cut(s) 118, 317
FriOI GRGCYC 2 cut(s) 672, 894
Fsp4HI GCNGC 3 cut(s) 246, 341, 824
FspBI CTAG 2 cut(s) 467, 735
GlaI GCGC 1 cut(s) 374
GluI GCNGC 3 cut(s) 246, 341, 824
GsuI CTGGAG 2 cut(s) 44, 105
HaeIII GGCC 3 cut(s) 72, 744, 949
HapII CCGG 1 cut(s) 785
HhaI GCGC 1 cut(s) 375
Hin1II CATG 4 cut(s) 20, 338, 645, 912
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 3 cut(s) 80, 626, 679
HpaII CCGG 1 cut(s) 785
HphI GGTGA 2 cut(s) 100, 709
Hpy166II GTNNAC 2 cut(s) 277, 394
Hpy188I TCNGA 6 cut(s) 239, 270, 577, 703, 817, 970
Hpy188III TCNNGA 6 cut(s) 17, 122, 188, 335, 382, 850
Hpy8I GTNNAC 2 cut(s) 277, 394
Hpy99I CGWCG 2 cut(s) 50, 330
HpyAV CCTTC 3 cut(s) 382, 544, 734
HpyCH4III ACNGT 2 cut(s) 135, 750
HpyCH4IV ACGT 3 cut(s) 777, 1014, 1021
HpyCH4V TGCA 6 cut(s) 410, 459, 507, 826, 857, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 803
HpyF3I CTNAG 3 cut(s) 236, 249, 614
HpySE526I ACGT 3 cut(s) 777, 1014, 1021
Hsp92II CATG 4 cut(s) 20, 338, 645, 912
HspAI GCGC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 538
Kzo9I GATC 4 cut(s) 13, 127, 184, 828
LmnI GCTCC 4 cut(s) 102, 216, 794, 958
Lsp1109I GCAGC 3 cut(s) 257, 352, 810
MaeI CTAG 2 cut(s) 467, 735
MaeII ACGT 3 cut(s) 777, 1014, 1021
MaeIII GTNAC 3 cut(s) 88, 750, 1022
MalI GATC 4 cut(s) 15, 129, 186, 830
MboI GATC 4 cut(s) 13, 127, 184, 828
MboII GAAGA 5 cut(s) 41, 203, 397, 419, 482
MfeI CAATTG 2 cut(s) 545, 903
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 3 cut(s) 672, 864, 894
MluCI AATT 7 cut(s) 41, 413, 545, 566, 570, 903, 984
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 3 cut(s) 141, 569, 953
MslI CAYNNNNRTG 3 cut(s) 150, 640, 762
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 785
MspR9I CCNGG 2 cut(s) 6, 347
MunI CAATTG 2 cut(s) 545, 903
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 2 cut(s) 6, 347
MvnI CGCG 3 cut(s) 214, 373, 375
MwoI GCNNNNNNNGC 1 cut(s) 803
NdeII GATC 4 cut(s) 13, 127, 184, 828
NlaIII CATG 4 cut(s) 20, 338, 645, 912
NlaIV GGNNCC 1 cut(s) 536
NmeAIII GCCGAG 2 cut(s) 663, 687
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 2 cut(s) 645, 912
NspV TTCGAA 1 cut(s) 624
PaeI GCATGC 1 cut(s) 645
PaeR7I CTCGAG 1 cut(s) 887
PagI TCATGA 2 cut(s) 16, 334
PceI AGGCCT 2 cut(s) 72, 744
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 204
PctI GAATGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 80, 626
PinAI ACCGGT 1 cut(s) 784
PkrI GCNGC 3 cut(s) 247, 342, 825
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 1015
PpuMI RGGWCCY 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 1021
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 4, 345
PspEI GGTNACC 1 cut(s) 88
PspGI CCWGG 2 cut(s) 4, 345
PspN4I GGNNCC 1 cut(s) 536
PspPI GGNCC 2 cut(s) 463, 947
PspPPI RGGWCCY 1 cut(s) 463
PspXI VCTCGAGB 1 cut(s) 887
PstI CTGCAG 2 cut(s) 509, 828
PsuI RGATCY 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 3 cut(s) 536, 873, 1013
RsaNI GTAC 3 cut(s) 535, 872, 1012
RseI CAYNNNNRTG 3 cut(s) 150, 640, 762
SaqAI TTAA 3 cut(s) 141, 569, 953
SatI GCNGC 3 cut(s) 246, 341, 824
Sau3AI GATC 4 cut(s) 13, 127, 184, 828
Sau96I GGNCC 2 cut(s) 463, 947
ScaI AGTACT 1 cut(s) 873
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 2 cut(s) 6, 347
SduI GDGCHC 3 cut(s) 672, 864, 894
SfcI CTRYAG 4 cut(s) 505, 746, 824, 1031
Sfr274I CTCGAG 1 cut(s) 887
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 1 cut(s) 463
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 3 cut(s) 150, 640, 762
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
SphI GCATGC 1 cut(s) 645
Sse9I AATT 7 cut(s) 41, 413, 545, 566, 570, 903, 984
SseBI AGGCCT 2 cut(s) 72, 744
SsiI CCGC 3 cut(s) 214, 512, 687
SspMI CTAG 2 cut(s) 467, 735
StuI AGGCCT 2 cut(s) 72, 744
StyD4I CCNGG 2 cut(s) 4, 345
StyI CCWWGG 2 cut(s) 101, 354
TaaI ACNGT 2 cut(s) 135, 750
TaiI ACGT 3 cut(s) 780, 1017, 1024
TaqI TCGA 8 cut(s) 45, 189, 198, 325, 486, 624, 629, 888
TasI AATT 7 cut(s) 41, 413, 545, 566, 570, 903, 984
TatI WGTACW 1 cut(s) 871
TfiI GAWTC 2 cut(s) 80, 626
Tru1I TTAA 3 cut(s) 141, 569, 953
Tru9I TTAA 3 cut(s) 141, 569, 953
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 3 cut(s) 245, 340, 823
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 5 cut(s) 50, 323, 703, 746, 904
VpaK11BI GGWCC 1 cut(s) 463
VspI ATTAAT 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 1 cut(s) 413
XceI RCATGY 2 cut(s) 645, 912
XhoI CTCGAG 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 2 cut(s) 467, 735
ZrmI AGTACT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.