pycom10g16150

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000837
Physical Location & Seq
Forward (+)
19322631 .. 19325855
3225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g16150.1

Sequence Viewer

Length: 1053 bp
ATGGACTGGGTGCCAACGCATTTTGTAGGGATGGATCGATCTCACATGGCAGCTCCGGATTACAGTGCCGAAAAGGAGTTGTACGCATTCGATGACCTCAAAATAGGCGTTAAAGGGCTCGTGGACTCTGGGGTGACCAAGATTCCCAGGATCTTCATCCACCCGCCGGAGAGCCTGAAGAACTCTCAGCCAGAAAATGGCGTTCACCTTCAAATCCCCGTCATTGATCTCAAAGGCTTTAATGAGACTGATGGCGGGCGAGAAGAGATTGTGAATGCCATTAAAGATGCGTGTGAAACGTGGGGTTTCTTTCAGATAGTTAACCATGGCGTTCCACTTGCTGTAATGGAGGAGATGCTGGAAGGTATAAGAAAATTTCACGAGCAGCCCCCGGAGGCTAAGAGGGAGTTGTACTCCCGTGACTATACGCGACACGTGAACTATTATTGCAGCGAAGATCTGAAGGTGCGTGCGAAATCAGCAGCAGACTGGAGAGACACCTTGTCCTTCAAGGCCGTAGATGATCACTGGGACTTTGAAGCACTTCCTCAAGTCTGCAGAAATGAAGTGAGTGAGTACACTGTCAGAAGCGTTAGGGCTCACCACGGATCACCTAGCGCAGATGGGGTGCTTGAAATCTTTCTCGTTATCATGCCACTACTACCCTGTTTGCCCGGAGCCCGATTTGACTCTTGGCACAATCAAACACTCAGACCCCTCCTTTCTCACCTTACTCCTGCAAGACAAGATTGGGGGCCTCCAACTTTAGTAGCAAACCTTGGTGACTTTATGCAGCTCGTTACTAATGACAAGTTCAAGAGTGTGGAGCACAGAGTACTCGCCACCCCACTTGTCAAGCCTCGAATATCAGTTGCATCTTTTCTCGTTCCTAGTGCCAAGGACAAACTCAACCCCTACAAGCCAATCAAGGAGCTTCTGTCTGAAACCAATCCACCCAAATACAGAGATACGCTTCACGGAGAATATACTGCGTACTACAGAGTCAATGGACAAAATGGTGGCTCTGCCCTGCCTCATTTTCAACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

351

Amino Acids

39.69

Weight (kDa)

6.15

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 72 - 173 9.7e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 255 - 296 9.5e-08 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 197
AccII CGCG 1 cut(s) 430
AccIII TCCGGA 1 cut(s) 55
AciI CCGC 2 cut(s) 164, 255
AclWI GGATC 3 cut(s) 42, 158, 616
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 2 cut(s) 197, 482
AcvI CACGTG 1 cut(s) 436
AfaI GTAC 5 cut(s) 83, 413, 578, 837, 995
AfiI CCNNNNNNNGG 2 cut(s) 166, 197
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 6 cut(s) 212, 511, 539, 635, 817, 1043
AhdI GACNNNNNGTC 1 cut(s) 502
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 3 cut(s) 53, 796, 934
AluI AGCT 3 cut(s) 53, 796, 934
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 2 cut(s) 239, 489
AlwI GGATC 3 cut(s) 42, 158, 616
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 2 cut(s) 513, 755
ApeKI GCWGC 5 cut(s) 50, 385, 450, 482, 793
ApoI RAATTY 1 cut(s) 374
Asp700I GAANNNNTTC 2 cut(s) 543, 639
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 2 cut(s) 392, 675
AsuHPI GGTGA 6 cut(s) 145, 197, 593, 603, 719, 794
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 3 cut(s) 120, 601, 682
BauI CACGAG 2 cut(s) 119, 380
BbrPI CACGTG 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 5 cut(s) 62, 397, 462, 494, 805
BccI CCATC 3 cut(s) 25, 245, 617
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 1 cut(s) 148
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 2 cut(s) 392, 675
BcoDI GTCTC 2 cut(s) 239, 489
BfaI CTAG 2 cut(s) 615, 891
BfmI CTRYAG 2 cut(s) 556, 997
BglII AGATCT 1 cut(s) 457
BisI GCNGC 5 cut(s) 51, 386, 451, 483, 794
BlsI GCNGC 5 cut(s) 52, 387, 452, 484, 795
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 3 cut(s) 148, 392, 675
BmeRI GACNNNNNGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 3 cut(s) 12, 679, 756
BmrFI CCNGG 3 cut(s) 148, 392, 675
BmrI ACTGGG 2 cut(s) 16, 538
BmsI GCATC 3 cut(s) 277, 345, 884
BmuI ACTGGG 2 cut(s) 16, 538
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpmI CTGGAG 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 2 cut(s) 392, 675
Bsa29I ATCGAT 1 cut(s) 37
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 155
BsaJI CCNNGG 6 cut(s) 146, 325, 390, 604, 778, 897
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 923, 953
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 197
Bse1I ACTGG 3 cut(s) 11, 494, 533
Bse8I GATNNNNATC 1 cut(s) 155
BseAI TCCGGA 1 cut(s) 55
BseBI CCWGG 1 cut(s) 148
BseCI ATCGAT 1 cut(s) 37
BseDI CCNNGG 6 cut(s) 146, 325, 390, 604, 778, 897
BseGI GGATG 2 cut(s) 36, 156
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 166, 197
BseMII CTCAG 2 cut(s) 200, 724
BseNI ACTGG 3 cut(s) 11, 494, 533
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 5 cut(s) 62, 397, 462, 494, 805
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 2 cut(s) 515, 757
BshNI GGYRCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 831
BsiSI CCGG 4 cut(s) 56, 167, 392, 675
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 166, 197
BsmAI GTCTC 2 cut(s) 239, 489
BsmFI GGGAC 1 cut(s) 545
BsmI GAATGC 2 cut(s) 86, 280
BsnI GGCC 2 cut(s) 515, 757
Bsp1286I GDGCHC 4 cut(s) 120, 601, 682, 831
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
Bsp19I CCATGG 1 cut(s) 325
BspACI CCGC 2 cut(s) 164, 255
BspANI GGCC 2 cut(s) 515, 757
BspCNI CTCAG 2 cut(s) 199, 723
BspDI ATCGAT 1 cut(s) 37
BspEI TCCGGA 1 cut(s) 55
BspFNI CGCG 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 12, 679, 756
BspMAI CTGCAG 1 cut(s) 560
BspPI GGATC 3 cut(s) 42, 158, 616
BspT107I GGYRCC 1 cut(s) 10
BsrI ACTGG 3 cut(s) 11, 494, 533
BssECI CCNNGG 6 cut(s) 146, 325, 390, 604, 778, 897
BssMI GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
BssSI CACGAG 2 cut(s) 119, 380
BssT1I CCWWGG 3 cut(s) 325, 778, 897
Bst2BI CACGAG 2 cut(s) 119, 380
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 2 cut(s) 65, 583
Bst6I CTCTTC 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 257, 471
BstDEI CTNAG 3 cut(s) 186, 399, 710
BstDSI CCRYGG 2 cut(s) 325, 604
BstEII GGTNACC 1 cut(s) 133
BstF5I GGATG 2 cut(s) 36, 156
BstFNI CGCG 1 cut(s) 430
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 7 cut(s) 37, 41, 153, 229, 460, 526, 611
BstMAI GTCTC 2 cut(s) 239, 489
BstMBI GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 148
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 3 cut(s) 146, 390, 673
BstSFI CTRYAG 2 cut(s) 556, 997
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 5 cut(s) 62, 397, 462, 494, 805
BstX2I RGATCY 2 cut(s) 150, 457
BstYI RGATCY 2 cut(s) 150, 457
Bsu15I ATCGAT 1 cut(s) 37
BsuRI GGCC 2 cut(s) 515, 757
BsuTUI ATCGAT 1 cut(s) 37
BtgI CCRYGG 2 cut(s) 325, 604
BtsCI GGATG 2 cut(s) 36, 156
BtsIMutI CAGTG 3 cut(s) 70, 526, 579
Cac8I GCNNGC 2 cut(s) 257, 471
CfoI GCGC 1 cut(s) 620
Cfr13I GGNCC 1 cut(s) 755
ClaI ATCGAT 1 cut(s) 37
Csp6I GTAC 5 cut(s) 82, 412, 577, 836, 994
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 3 cut(s) 46, 326, 652
CviQI GTAC 5 cut(s) 82, 412, 577, 836, 994
DdeI CTNAG 3 cut(s) 186, 399, 710
DpnI GATC 7 cut(s) 36, 40, 152, 228, 459, 525, 610
DpnII GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
DriI GACNNNNNGTC 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 258
Eam1105I GACNNNNNGTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 3 cut(s) 325, 778, 897
Eco24I GRGCYC 3 cut(s) 120, 601, 682
Eco57I CTGAAG 2 cut(s) 197, 482
Eco72I CACGTG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 755
EcoO65I GGTNACC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 3 cut(s) 325, 778, 897
EcoT38I GRGCYC 3 cut(s) 120, 601, 682
ErhI CCWWGG 3 cut(s) 325, 778, 897
FaeI CATG 3 cut(s) 49, 329, 655
FaiI YATR 7 cut(s) 47, 327, 368, 426, 653, 791, 987
FaqI GGGAC 1 cut(s) 545
FatI CATG 3 cut(s) 45, 325, 651
FauI CCCGC 2 cut(s) 171, 248
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 5 cut(s) 51, 386, 451, 483, 794
FokI GGATG 2 cut(s) 43, 143
FriOI GRGCYC 3 cut(s) 120, 601, 682
Fsp4HI GCNGC 5 cut(s) 51, 386, 451, 483, 794
FspBI CTAG 2 cut(s) 615, 891
GlaI GCGC 1 cut(s) 619
GluI GCNGC 5 cut(s) 51, 386, 451, 483, 794
GsuI CTGGAG 1 cut(s) 511
HaeIII GGCC 2 cut(s) 515, 757
HapII CCGG 4 cut(s) 56, 167, 392, 675
HhaI GCGC 1 cut(s) 620
Hin1II CATG 3 cut(s) 49, 329, 655
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 4 cut(s) 125, 142, 689, 1002
HpaI GTTAAC 1 cut(s) 322
HpaII CCGG 4 cut(s) 56, 167, 392, 675
HphI GGTGA 6 cut(s) 145, 197, 593, 603, 719, 794
Hpy166II GTNNAC 5 cut(s) 124, 205, 322, 439, 579
Hpy188I TCNGA 5 cut(s) 315, 462, 587, 713, 943
Hpy188III TCNNGA 3 cut(s) 56, 380, 817
Hpy8I GTNNAC 5 cut(s) 124, 205, 322, 439, 579
HpyAV CCTTC 4 cut(s) 218, 356, 457, 517
HpyCH4III ACNGT 2 cut(s) 65, 583
HpyCH4IV ACGT 2 cut(s) 299, 435
HpyCH4V TGCA 6 cut(s) 450, 558, 740, 793, 875, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 3 cut(s) 186, 399, 710
HpySE526I ACGT 2 cut(s) 299, 435
Hsp92II CATG 3 cut(s) 49, 329, 655
HspAI GCGC 1 cut(s) 618
Kpn2I TCCGGA 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
LmnI GCTCC 4 cut(s) 58, 677, 826, 931
Lsp1109I GCAGC 5 cut(s) 62, 397, 462, 494, 805
LweI GCATC 3 cut(s) 277, 345, 884
MaeI CTAG 2 cut(s) 615, 891
MaeII ACGT 2 cut(s) 299, 435
MaeIII GTNAC 4 cut(s) 133, 419, 782, 799
MalI GATC 7 cut(s) 36, 40, 152, 228, 459, 525, 610
MboI GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
MboII GAAGA 4 cut(s) 145, 190, 275, 467
MflI RGATCY 2 cut(s) 150, 457
MhlI GDGCHC 4 cut(s) 120, 601, 682, 831
MluCI AATT 1 cut(s) 374
MlyI GAGTC 3 cut(s) 119, 683, 1011
MmeI TCCRAC 1 cut(s) 785
MnlI CCTC 9 cut(s) 107, 343, 388, 396, 558, 728, 768, 870, 1044
MroI TCCGGA 1 cut(s) 55
MroXI GAANNNNTTC 2 cut(s) 543, 639
MseI TTAA 4 cut(s) 111, 240, 282, 321
MspI CCGG 4 cut(s) 56, 167, 392, 675
MspR9I CCNGG 3 cut(s) 148, 392, 675
Mva1269I GAATGC 2 cut(s) 86, 280
MvaI CCWGG 1 cut(s) 148
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 1 cut(s) 479
NciI CCSGG 2 cut(s) 392, 675
NcoI CCATGG 1 cut(s) 325
NdeII GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
NlaIII CATG 3 cut(s) 49, 329, 655
NlaIV GGNNCC 3 cut(s) 12, 679, 756
NmuCI GTSAC 3 cut(s) 133, 419, 782
PctI GAATGC 2 cut(s) 86, 280
PdmI GAANNNNTTC 2 cut(s) 543, 639
PfeI GAWTC 1 cut(s) 142
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 5 cut(s) 52, 387, 452, 484, 795
PleI GAGTC 3 cut(s) 119, 683, 1010
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
PpsI GAGTC 3 cut(s) 119, 683, 1010
Ppu21I YACGTR 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 146
PspCI CACGTG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 3 cut(s) 12, 679, 756
PspPI GGNCC 1 cut(s) 755
PstI CTGCAG 1 cut(s) 560
PsuI RGATCY 2 cut(s) 150, 457
RsaI GTAC 5 cut(s) 83, 413, 578, 837, 995
RsaNI GTAC 5 cut(s) 82, 412, 577, 836, 994
SaqAI TTAA 4 cut(s) 111, 240, 282, 321
SatI GCNGC 5 cut(s) 51, 386, 451, 483, 794
Sau3AI GATC 7 cut(s) 34, 38, 150, 226, 457, 523, 608
Sau96I GGNCC 1 cut(s) 755
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 3 cut(s) 119, 683, 1011
ScrFI CCNGG 3 cut(s) 148, 392, 675
SduI GDGCHC 4 cut(s) 120, 601, 682, 831
SfaNI GCATC 3 cut(s) 277, 345, 884
SfcI CTRYAG 2 cut(s) 556, 997
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 1 cut(s) 374
SsiI CCGC 2 cut(s) 164, 255
SspMI CTAG 2 cut(s) 615, 891
StyD4I CCNGG 3 cut(s) 146, 390, 673
StyI CCWWGG 3 cut(s) 325, 778, 897
TaaI ACNGT 2 cut(s) 65, 583
TaiI ACGT 2 cut(s) 302, 438
TaqI TCGA 3 cut(s) 37, 90, 862
TasI AATT 1 cut(s) 374
TatI WGTACW 3 cut(s) 411, 576, 835
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 4 cut(s) 111, 240, 282, 321
Tru9I TTAA 4 cut(s) 111, 240, 282, 321
TscAI CASTG 3 cut(s) 70, 533, 586
TseFI GTSAC 3 cut(s) 133, 419, 782
TseI GCWGC 5 cut(s) 50, 385, 450, 482, 793
Tsp45I GTSAC 3 cut(s) 133, 419, 782
TspDTI ATGAA 2 cut(s) 145, 579
TspGWI ACGGA 2 cut(s) 621, 993
TspRI CASTG 3 cut(s) 70, 533, 586
Van91I CCANNNNNTGG 1 cut(s) 197
XapI RAATTY 1 cut(s) 374
XmnI GAANNNNTTC 2 cut(s) 543, 639
XspI CTAG 2 cut(s) 615, 891
ZrmI AGTACT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.