FvH4_2g21910

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000837
Physical Location & Seq
Reverse (-)
18095175 .. 18097582
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21910.t1

Sequence Viewer

Length: 1137 bp
ATGATCGAAGGAGTCCATACTCATCTTCAACCTGCAGACTTGTTCTTGGCTAACAATATTCCAAACACTAAGATGGCAGCGGTTGTGGATTATGATCTGCGTGAGAAGGAGCTGAAGGCATTCGACGAAAGCAAAGCTGGCGTCAAAGGATTTGTAGACGCTGGAGTGACCAAGCTCCCAGGCATGTTCATGCATCCTCCAGAGAATCTGCTGAACTCTCCATTCCCGGGAGAAGAAGAGCAGTATCAGAAAGTTCGTAGCCTACAATTTCCGGTGATTGATCTTAGCGGTTTTGATAACAGCGAGAAGAGAAAAGAAATCATCAATGACATAAACAAAGCAGCTGAATCATGGGGATTCTTTCAGCTGGTGAACCATGGAATCCCACTTGAAGCTATGGAGGGGATCCAGAGAAGTATCCGAGAGTTTCATGAGCTTCCCCAGGAGGAGAAGGCCAAGTGGTACTCGCGCGATTTTACGAAGAAGGTTAACTTCTTCAGCTTTCATGGAGATTTGAAGGTGACGACGCCAGCTGACTGGAGGGATACCTTGTCATGCAAGGTCCTAGAAGACCCGAAAAGCTTTGAAGAAATACCACAAGTTTGCAGAGGAGAAGTGAGTGAGTATATGAAACACATCGATGGAGTGCTACAGAAGTTATCTGAGTTATTTTCAGAAGCTTTAGGTCTTAGTAGTGATTATCTTGCAAGCACAAGGTGCTTCAGAGCGAGGTCATTGGCGTGCCACTATTTCCCGATATGCCCGGAACCCCATCTGACCATGGGGGGAACTAAGCATACGGACCTCGGCTTTCTGACGTTGCTCCTCCAAGACAGCGTCGGCGGCCTACAAGTTCGTCATCAAGGTTTTTGGATTGATGTTCCTCCGGTGAAGGGAGCTTTGCTAGCAAATCTTGCTGACATGATGGAGATAGTTACAAATGGTAAGTTTAAGAGCGTGGAACACAGAGTTTTGTTGAAACCAACTGTGGAGCCTCGTATCTCAATTGCATGTTTCTTGAGTACAGACGACCTCGAAAAATCATATGGGCCGATAAAAGAGCTCATATCGGAAGACAATCCGCCGAAGTTCAGCGAAGTCAAATTCGGAGAATACATGAAAAAGTATAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

379

Amino Acids

42.86

Weight (kDa)

5.71

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 91 - 191 8e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 246 - 341 1.2e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 156
AccII CGCG 2 cut(s) 469, 471
AciI CCGC 4 cut(s) 80, 288, 843, 1082
AclWI GGATC 2 cut(s) 400, 413
AcsI RAATTY 1 cut(s) 1103
AcuI CTGAAG 3 cut(s) 134, 481, 706
AcyI GRCGYC 2 cut(s) 141, 527
AdeI CACNNNGTG 1 cut(s) 717
AfaI GTAC 2 cut(s) 464, 1024
AfiI CCNNNNNNNGG 2 cut(s) 227, 893
AgsI TTSAA 5 cut(s) 29, 392, 517, 587, 979
AjnI CCWGG 2 cut(s) 178, 441
Alw21I GWGCWC 1 cut(s) 1065
AlwI GGATC 2 cut(s) 400, 413
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 3 cut(s) 453, 844, 1049
ApeKI GCWGC 2 cut(s) 77, 341
ApoI RAATTY 1 cut(s) 1103
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 3 cut(s) 562, 802, 1049
AsuC2I CCSGG 3 cut(s) 227, 228, 764
AsuHPI GGTGA 4 cut(s) 286, 382, 532, 901
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 2 cut(s) 562, 802
BamHI GGATCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 1065
BbsI GAAGAC 2 cut(s) 576, 1080
Bbv12I GWGCWC 1 cut(s) 1065
BbvI GCAGC 2 cut(s) 89, 353
BccI CCATC 4 cut(s) 67, 635, 780, 919
BciT130I CCWGG 2 cut(s) 180, 443
BciVI GTATCC 2 cut(s) 428, 538
BcnI CCSGG 3 cut(s) 227, 228, 764
BfaI CTAG 2 cut(s) 566, 905
BfmI CTRYAG 2 cut(s) 33, 650
BfuAI ACCTGC 1 cut(s) 40
BfuI GTATCC 2 cut(s) 428, 538
BisI GCNGC 3 cut(s) 78, 342, 844
BlsI GCNGC 3 cut(s) 79, 343, 845
Bme1390I CCNGG 5 cut(s) 180, 227, 228, 443, 764
Bme18I GGWCC 2 cut(s) 562, 802
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 562, 802, 1049
BmiI GGNNCC 3 cut(s) 407, 768, 993
BmrFI CCNGG 5 cut(s) 180, 227, 228, 443, 764
BmsI GCATC 1 cut(s) 202
BmtI GCTAGC 1 cut(s) 908
BpiI GAAGAC 2 cut(s) 576, 1080
BpmI CTGGAG 3 cut(s) 183, 183, 559
BpuEI CTTGAG 1 cut(s) 1039
BpuMI CCSGG 3 cut(s) 227, 228, 764
Bsa29I ATCGAT 1 cut(s) 639
BsaBI GATNNNNATC 1 cut(s) 93
BsaHI GRCGYC 2 cut(s) 141, 527
BsaJI CCNNGG 6 cut(s) 178, 226, 376, 441, 780, 805
BsaWI WCCGGW 2 cut(s) 271, 886
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 893
Bse1I ACTGG 1 cut(s) 542
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 2 cut(s) 180, 443
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 6 cut(s) 178, 226, 376, 441, 780, 805
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 227, 893
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 3 cut(s) 461, 624, 815
BseXI GCAGC 2 cut(s) 89, 353
Bsh1236I CGCG 2 cut(s) 469, 471
BshFI GGCC 3 cut(s) 455, 846, 1051
BshVI ATCGAT 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 1065
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 4 cut(s) 227, 272, 764, 887
BslI CCNNNNNNNGG 2 cut(s) 227, 893
BsmI GAATGC 1 cut(s) 119
BsnI GGCC 3 cut(s) 455, 846, 1051
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 1065
Bsp143I GATC 4 cut(s) 3, 94, 280, 405
Bsp19I CCATGG 2 cut(s) 376, 780
BspACI CCGC 4 cut(s) 80, 288, 843, 1082
BspANI GGCC 3 cut(s) 455, 846, 1051
BspCNI CTCAG 1 cut(s) 655
BspDI ATCGAT 1 cut(s) 639
BspFNI CGCG 2 cut(s) 469, 471
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 407, 768, 993
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 40
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 2 cut(s) 400, 413
BspQI GCTCTTC 1 cut(s) 231
BsrI ACTGG 1 cut(s) 542
BssECI CCNNGG 6 cut(s) 178, 226, 376, 441, 780, 805
BssMI GATC 4 cut(s) 3, 94, 280, 405
BssNI GRCGYC 2 cut(s) 141, 527
BssT1I CCWWGG 2 cut(s) 376, 780
Bst2UI CCWGG 2 cut(s) 180, 443
Bst4CI ACNGT 1 cut(s) 988
Bst6I CTCTTC 2 cut(s) 231, 302
BstACI GRCGYC 2 cut(s) 141, 527
BstAPI GCANNNNNTGC 2 cut(s) 717, 914
BstC8I GCNNGC 5 cut(s) 139, 531, 709, 742, 906
BstDEI CTNAG 5 cut(s) 69, 284, 663, 689, 792
BstDSI CCRYGG 2 cut(s) 376, 780
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 2 cut(s) 469, 471
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 4 cut(s) 6, 97, 283, 408
BstMBI GATC 4 cut(s) 3, 94, 280, 405
BstMWI GCNNNNNNNGC 5 cut(s) 138, 717, 843, 905, 914
BstNI CCWGG 2 cut(s) 180, 443
BstNSI RCATGY 2 cut(s) 187, 1014
BstSCI CCNGG 5 cut(s) 178, 225, 226, 441, 762
BstSFI CTRYAG 2 cut(s) 33, 650
BstUI CGCG 2 cut(s) 469, 471
BstV1I GCAGC 2 cut(s) 89, 353
BstV2I GAAGAC 2 cut(s) 576, 1080
BstX2I RGATCY 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 537
BstYI RGATCY 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 639
BsuI GTATCC 2 cut(s) 428, 538
BsuRI GGCC 3 cut(s) 455, 846, 1051
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 2 cut(s) 376, 780
BtsCI GGATG 1 cut(s) 193
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 5 cut(s) 139, 531, 709, 742, 906
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 471
Cfr13I GGNCC 3 cut(s) 562, 802, 1049
Cfr9I CCCGGG 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 639
CseI GACGC 4 cut(s) 130, 167, 535, 826
Csp6I GTAC 2 cut(s) 463, 1023
CviQI GTAC 2 cut(s) 463, 1023
DdeI CTNAG 5 cut(s) 69, 284, 663, 689, 792
DpnI GATC 4 cut(s) 5, 96, 282, 407
DpnII GATC 4 cut(s) 3, 94, 280, 405
DraIII CACNNNGTG 1 cut(s) 717
Eam1104I CTCTTC 2 cut(s) 231, 302
EarI CTCTTC 2 cut(s) 231, 302
EciI GGCGGA 1 cut(s) 1071
Ecl136II GAGCTC 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 376, 780
Eco24I GRGCYC 1 cut(s) 1065
Eco47I GGWCC 2 cut(s) 562, 802
Eco53kI GAGCTC 1 cut(s) 1063
Eco57I CTGAAG 3 cut(s) 134, 481, 706
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 1063
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 178, 441
EcoT14I CCWWGG 2 cut(s) 376, 780
EcoT22I ATGCAT 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 1065
ErhI CCWWGG 2 cut(s) 376, 780
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FauNDI CATATG 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 78, 342, 844
FokI GGATG 1 cut(s) 180
FriOI GRGCYC 1 cut(s) 1065
Fsp4HI GCNGC 3 cut(s) 78, 342, 844
FspBI CTAG 2 cut(s) 566, 905
GlaI GCGC 1 cut(s) 470
GluI GCNGC 3 cut(s) 78, 342, 844
GsuI CTGGAG 3 cut(s) 183, 183, 559
HaeIII GGCC 3 cut(s) 455, 846, 1051
HapII CCGG 4 cut(s) 227, 272, 764, 887
HgaI GACGC 4 cut(s) 130, 167, 535, 826
HhaI GCGC 1 cut(s) 471
Hin1I GRCGYC 2 cut(s) 141, 527
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 580, 678
HinfI GANTC 5 cut(s) 12, 205, 347, 357, 381
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 4 cut(s) 227, 272, 764, 887
HphI GGTGA 4 cut(s) 286, 382, 532, 901
Hpy166II GTNNAC 3 cut(s) 157, 373, 490
Hpy188I TCNGA 9 cut(s) 249, 422, 664, 676, 725, 777, 816, 1072, 1109
Hpy188III TCNNGA 5 cut(s) 200, 409, 431, 754, 1018
Hpy8I GTNNAC 3 cut(s) 157, 373, 490
Hpy99I CGWCG 3 cut(s) 128, 529, 842
HpyAV CCTTC 6 cut(s) 100, 109, 445, 478, 511, 886
HpyCH4III ACNGT 1 cut(s) 988
HpyCH4IV ACGT 1 cut(s) 818
HpyCH4V TGCA 6 cut(s) 35, 193, 558, 606, 707, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 717, 843, 905, 914
HpyF3I CTNAG 5 cut(s) 69, 284, 663, 689, 792
HpySE526I ACGT 1 cut(s) 818
Hsp92I GRCGYC 2 cut(s) 141, 527
HspAI GCGC 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 4 cut(s) 3, 94, 280, 405
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 5 cut(s) 109, 180, 828, 896, 991
Lsp1109I GCAGC 2 cut(s) 89, 353
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 566, 905
MaeII ACGT 1 cut(s) 818
MaeIII GTNAC 3 cut(s) 166, 520, 934
MalI GATC 4 cut(s) 5, 96, 282, 407
MboI GATC 4 cut(s) 3, 94, 280, 405
MboII GAAGA 9 cut(s) 17, 245, 248, 319, 487, 493, 581, 599, 1085
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 1 cut(s) 1065
MluCI AATT 3 cut(s) 266, 1005, 1103
MlyI GAGTC 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 2 cut(s) 489, 951
MslI CAYNNNNRTG 4 cut(s) 71, 188, 639, 739
MspA1I CMGCKG 4 cut(s) 80, 344, 367, 533
MspI CCGG 4 cut(s) 227, 272, 764, 887
MspR9I CCNGG 5 cut(s) 180, 227, 228, 443, 764
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 2 cut(s) 180, 443
MvnI CGCG 2 cut(s) 469, 471
MwoI GCNNNNNNNGC 5 cut(s) 138, 717, 843, 905, 914
NciI CCSGG 3 cut(s) 227, 228, 764
NcoI CCATGG 2 cut(s) 376, 780
NdeI CATATG 1 cut(s) 1045
NdeII GATC 4 cut(s) 3, 94, 280, 405
NheI GCTAGC 1 cut(s) 904
NlaIV GGNNCC 3 cut(s) 407, 768, 993
NmeAIII GCCGAG 1 cut(s) 786
NmuCI GTSAC 2 cut(s) 166, 520
NsiI ATGCAT 1 cut(s) 195
NspI RCATGY 2 cut(s) 187, 1014
PagI TCATGA 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 231
PctI GAATGC 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 4 cut(s) 205, 347, 357, 381
PflFI GACNNNGTC 1 cut(s) 836
PkrI GCNGC 3 cut(s) 79, 343, 845
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 562
Psp124BI GAGCTC 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 178, 441
PspGI CCWGG 2 cut(s) 178, 441
PspN4I GGNNCC 3 cut(s) 407, 768, 993
PspPI GGNCC 3 cut(s) 562, 802, 1049
PspPPI RGGWCCY 1 cut(s) 562
PsrI GAACNNNNNNTAC 2 cut(s) 781, 813
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 836
PvuII CAGCTG 3 cut(s) 344, 367, 533
RsaI GTAC 2 cut(s) 464, 1024
RsaNI GTAC 2 cut(s) 463, 1023
RseI CAYNNNNRTG 4 cut(s) 71, 188, 639, 739
SacI GAGCTC 1 cut(s) 1065
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 489, 951
SatI GCNGC 3 cut(s) 78, 342, 844
Sau3AI GATC 4 cut(s) 3, 94, 280, 405
Sau96I GGNCC 3 cut(s) 562, 802, 1049
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 5 cut(s) 180, 227, 228, 443, 764
SduI GDGCHC 1 cut(s) 1065
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 2 cut(s) 33, 650
SinI GGWCC 2 cut(s) 562, 802
SmaI CCCGGG 1 cut(s) 228
SmiMI CAYNNNNRTG 4 cut(s) 71, 188, 639, 739
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
Sse9I AATT 3 cut(s) 266, 1005, 1103
SsiI CCGC 4 cut(s) 80, 288, 843, 1082
SspI AATATT 1 cut(s) 58
SspMI CTAG 2 cut(s) 566, 905
SstI GAGCTC 1 cut(s) 1065
StyD4I CCNGG 5 cut(s) 178, 225, 226, 441, 762
StyI CCWWGG 2 cut(s) 376, 780
TaaI ACNGT 1 cut(s) 988
TaiI ACGT 1 cut(s) 821
TaqI TCGA 4 cut(s) 6, 123, 639, 1035
TasI AATT 3 cut(s) 266, 1005, 1103
TatI WGTACW 1 cut(s) 1022
TauI GCSGC 1 cut(s) 846
TfiI GAWTC 4 cut(s) 205, 347, 357, 381
Tru1I TTAA 2 cut(s) 489, 951
Tru9I TTAA 2 cut(s) 489, 951
TseFI GTSAC 2 cut(s) 166, 520
TseI GCWGC 2 cut(s) 77, 341
Tsp45I GTSAC 2 cut(s) 166, 520
TspDTI ATGAA 5 cut(s) 178, 419, 494, 644, 1133
TspGWI ACGGA 1 cut(s) 815
TspMI CCCGGG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 562, 802
XapI RAATTY 1 cut(s) 1103
XceI RCATGY 2 cut(s) 187, 1014
XmaI CCCGGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 2 cut(s) 566, 905
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.