Rorug06G0197500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000837
Physical Location & Seq
Reverse (-)
31820486 .. 31821268
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0197500.1

Sequence Viewer

Length: 783 bp
ATGTACTGGCACCAGAGATCGAGGATTAACTGGCTTAATCTTGGTGACCAAAATTCTAGATTTTTCCATCAGACCACCCTTCAGAGAAGACAGTACAACAAAATTCTGCGCATTCAAGATCATGCTGGACATTGGTTGGATAGCGCGGCTGATATCAGCATGCTTTTCACTCTGCACTTTCAACAGCTCTACACTTCAAATGGCCCTCAAATCTTGGATGAAGTTCTAAACTTTGTCGATCCGCTCGTGACTCCAGATATGAACACGTCCTTAACTTCTCCTGTTACTCTCCAAGAAGTCAAGGATGCTGTTTTTGACTTGGGGGGCTTTAAAGCACCGGGACCTGATGGCTTTTCTGGCATTTTTTATCAAACTCATTGGGATATAGTCAAGTCTGTTGTCCATGAATCTGCTATCAACCATCTTGCTGCACAAAACATTCTCTCTCACTTTAACCAAACTCATATAGCCTTAGTTCCCAAGGTCCAGTGCCCTGTTTCTGCCTCACAATACCGACCTATTGCTTTATGCAATTTCTCATACAAAATCCTTGCCAAAATTATGGTAAGCAGACTAAAGCCTTTCATGTCTGACTTAATCACTGAGAATCAGTCAGCTTTTATCCAACAGAGGCAAATCCAAGACAACATCATTGTTGCTCATGAAGCATTCCACCATCTCAAACTGCTTCGCTCTGGTCATGATGGTGCTTTTGGGTTGAAATTAGATATGAGCAAAGCTTTTGACAGAGTGGAATGGACCTTCCTAGATGCTGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

260

Amino Acids

29.79

Weight (kDa)

6.92

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 161 - 258 3.3e-14 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 9
AccBSI CCGCTC 1 cut(s) 244
AccII CGCG 1 cut(s) 146
AciI CCGC 2 cut(s) 146, 242
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 2 cut(s) 52, 102
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 5, 95
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 4 cut(s) 116, 182, 198, 721
AjiI CACGTC 1 cut(s) 267
AluBI AGCT 3 cut(s) 187, 617, 740
AluI AGCT 3 cut(s) 187, 617, 740
AlwI GGATC 1 cut(s) 233
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 2 cut(s) 52, 102
AspLEI GCGC 2 cut(s) 111, 146
AspS9I GGNCC 4 cut(s) 203, 341, 484, 759
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 56
AvaII GGWCC 3 cut(s) 341, 484, 759
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 9
BauI CACGAG 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 415
BccI CCATC 5 cut(s) 75, 341, 429, 684, 698
BcnI CCSGG 1 cut(s) 339
BfaI CTAG 2 cut(s) 57, 767
BisI GCNGC 2 cut(s) 147, 429
BlsI GCNGC 2 cut(s) 148, 430
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 3 cut(s) 341, 484, 759
BmgBI CACGTC 1 cut(s) 267
BmgT120I GGNCC 4 cut(s) 203, 341, 484, 759
BmiI GGNNCC 2 cut(s) 11, 342
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 295, 760
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 480
Bse1I ACTGG 3 cut(s) 11, 35, 487
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 2 cut(s) 223, 310
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 3 cut(s) 11, 35, 487
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 1 cut(s) 415
BsgI GTGCAG 2 cut(s) 158, 414
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 354
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 2 cut(s) 111, 668
BsnI GGCC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 3 cut(s) 17, 118, 238
BspACI CCGC 2 cut(s) 146, 242
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 595
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 2 cut(s) 661, 700
BspLI GGNNCC 2 cut(s) 11, 342
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 9
BsrBI CCGCTC 1 cut(s) 244
BsrI ACTGG 3 cut(s) 11, 35, 487
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 3 cut(s) 17, 118, 238
BssSI CACGAG 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 472, 603
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 2 cut(s) 223, 310
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 111, 146
BstKTI GATC 3 cut(s) 20, 121, 241
BstMBI GATC 3 cut(s) 17, 118, 238
BstMWI GCNNNNNNNGC 2 cut(s) 357, 665
BstNSI RCATGY 1 cut(s) 163
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 562
BsuRI GGCC 1 cut(s) 204
BtrI CACGTC 1 cut(s) 267
BtsCI GGATG 2 cut(s) 223, 310
BtsIMutI CAGTG 2 cut(s) 494, 600
Cac8I GCNNGC 1 cut(s) 161
CciI TCATGA 2 cut(s) 661, 700
CfoI GCGC 2 cut(s) 111, 146
Cfr13I GGNCC 4 cut(s) 203, 341, 484, 759
Csp6I GTAC 2 cut(s) 4, 94
CviAII CATG 6 cut(s) 122, 160, 404, 586, 662, 701
CviQI GTAC 2 cut(s) 4, 94
DdeI CTNAG 2 cut(s) 472, 603
DpnI GATC 3 cut(s) 19, 120, 240
DpnII GATC 3 cut(s) 17, 118, 238
DraI TTTAAA 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 480
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 3 cut(s) 341, 484, 759
Eco57I CTGAAG 1 cut(s) 65
Eco91I GGTNACC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 44
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 6 cut(s) 125, 163, 407, 589, 665, 704
FaqI GGGAC 1 cut(s) 354
FatI CATG 6 cut(s) 121, 159, 403, 585, 661, 700
Fnu4HI GCNGC 2 cut(s) 147, 429
FokI GGATG 2 cut(s) 230, 317
Fsp4HI GCNGC 2 cut(s) 147, 429
FspBI CTAG 2 cut(s) 57, 767
FspI TGCGCA 1 cut(s) 110
GlaI GCGC 2 cut(s) 110, 145
GluI GCNGC 2 cut(s) 147, 429
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 338
HhaI GCGC 2 cut(s) 111, 146
Hin1II CATG 6 cut(s) 125, 163, 407, 589, 665, 704
Hin6I GCGC 2 cut(s) 109, 144
HinP1I GCGC 2 cut(s) 109, 144
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 3 cut(s) 250, 407, 607
HpaII CCGG 1 cut(s) 338
HphI GGTGA 1 cut(s) 56
Hpy188I TCNGA 4 cut(s) 72, 84, 592, 782
Hpy188III TCNNGA 6 cut(s) 57, 116, 247, 254, 662, 701
HpyAV CCTTC 2 cut(s) 89, 772
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 175, 431, 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 665
HpyF3I CTNAG 2 cut(s) 472, 603
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 6 cut(s) 125, 163, 407, 589, 665, 704
HspAI GCGC 2 cut(s) 109, 144
Kzo9I GATC 3 cut(s) 17, 118, 238
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 295, 760
MaeI CTAG 2 cut(s) 57, 767
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 44, 247, 283
MalI GATC 3 cut(s) 19, 120, 240
MbiI CCGCTC 1 cut(s) 244
MboI GATC 3 cut(s) 17, 118, 238
MboII GAAGA 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 5 cut(s) 52, 102, 532, 558, 722
MlyI GAGTC 1 cut(s) 244
MmeI TCCRAC 2 cut(s) 117, 649
MnlI CCTC 4 cut(s) 15, 216, 514, 624
MseI TTAA 6 cut(s) 27, 36, 272, 330, 453, 596
MslI CAYNNNNRTG 1 cut(s) 705
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 111, 668
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 2 cut(s) 357, 665
NciI CCSGG 1 cut(s) 339
NdeII GATC 3 cut(s) 17, 118, 238
NlaIII CATG 6 cut(s) 125, 163, 407, 589, 665, 704
NlaIV GGNNCC 2 cut(s) 11, 342
NmuCI GTSAC 2 cut(s) 44, 247
NsbI TGCGCA 1 cut(s) 110
NspI RCATGY 1 cut(s) 163
PaeI GCATGC 1 cut(s) 163
PagI TCATGA 2 cut(s) 661, 700
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 2 cut(s) 111, 668
PfeI GAWTC 2 cut(s) 407, 607
PkrI GCNGC 2 cut(s) 148, 430
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PpuMI RGGWCCY 1 cut(s) 341
Psp5II RGGWCCY 1 cut(s) 341
PspEI GGTNACC 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 11, 342
PspPI GGNCC 4 cut(s) 203, 341, 484, 759
PspPPI RGGWCCY 1 cut(s) 341
RsaI GTAC 2 cut(s) 5, 95
RsaNI GTAC 2 cut(s) 4, 94
RseI CAYNNNNRTG 1 cut(s) 705
SaqAI TTAA 6 cut(s) 27, 36, 272, 330, 453, 596
SatI GCNGC 2 cut(s) 147, 429
Sau3AI GATC 3 cut(s) 17, 118, 238
Sau96I GGNCC 4 cut(s) 203, 341, 484, 759
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 494
SetI ASST 8 cut(s) 189, 269, 346, 486, 520, 619, 742, 764
SfaNI GCATC 2 cut(s) 295, 760
SinI GGWCC 3 cut(s) 341, 484, 759
SmiMI CAYNNNNRTG 1 cut(s) 705
SphI GCATGC 1 cut(s) 163
Sse9I AATT 5 cut(s) 52, 102, 532, 558, 722
SsiI CCGC 2 cut(s) 146, 242
SspMI CTAG 2 cut(s) 57, 767
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 1 cut(s) 269
TaqI TCGA 2 cut(s) 20, 237
TasI AATT 5 cut(s) 52, 102, 532, 558, 722
TatI WGTACW 2 cut(s) 3, 93
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 2 cut(s) 407, 607
Tru1I TTAA 6 cut(s) 27, 36, 272, 330, 453, 596
Tru9I TTAA 6 cut(s) 27, 36, 272, 330, 453, 596
TscAI CASTG 2 cut(s) 494, 607
TseFI GTSAC 2 cut(s) 44, 247
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 2 cut(s) 44, 247
TspDTI ATGAA 5 cut(s) 234, 275, 420, 574, 678
TspRI CASTG 2 cut(s) 494, 607
VpaK11BI GGWCC 3 cut(s) 341, 484, 759
XapI RAATTY 2 cut(s) 52, 102
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 163
XspI CTAG 2 cut(s) 57, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.