Rorug06G0197700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000837
Physical Location & Seq
Reverse (-)
31841106 .. 31850116
9011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0197700.1

Sequence Viewer

Length: 1218 bp
ATGTCGCCAAACTTCAAGCTTCTTCAGATTGACGAGGAATCAAATATCTTCGATGGAGTGAAGCATGTCGTCGATCTCTGTGCTGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAATTGTATCTGCCAGCAAAGCTTTTGTCAGATTCTAACCCCGTCAATGGATTCAACTCGTCTGCTCCGGAATTCACACCCTTTTACATTTACACTCGCTCCACCCATCACCAAAACCCGCCATTACTCACTCTCTCTCCCCTCCCGCAAACTCTTCTTCATGCTCAAAACGACGTCGGCCCCTTCGACGACGCAGCTTACGAGGCCGAGCGGCGCAGCCTCGACGCCAAGGCTCGGCAAACCATGGCTGAGGAGGCTTCCAGTGTTACGGAAGACCCCAAAGCTTGGAAATGGGTGATCCGGAAGAGAATTTGGGACATGATGGAGGCCCGCAACATAGCCCAGAACCCAAGGCCTGTTCACCACCGCATCCCCAACTTCGTCGGCGCCTCCGCTGCTGCCGATAAATTGGGCGGGTTGGAAGCGTTCCGTGTCGGGAACTGTGTGAAAGTGAACCCGGATTCTCCTCAGAAGCAAGTCAGGTTTCTCACACTCTCTGATGGGAAGAAGCTGTTAACTCCTCAACCCCGTTTACGGACTGGGTTTTTCTCTGTGCTTGAATCTGAAATGTTGACTCCTAGTACTATGAAAGAGGCTTGCACCTCTGTTGGGGTGGCCAAACACGGGCGGCCTATTGGATTAGATGAGAAAATCAAGGTTGATCTTATTGTCATTGGCTCCGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCAGAGCTTGAATATGGGATGCTGCGATACATGGGAGCCATTGATGATTCAACACCAGTTGTCACTACTGTGCATGACAGTCAATTAGTAGATGATATTCCAATTGAGAAGCTGTTAGTCCATGATGTACCAGTTGACATCATATGCACTCCAACCCAGGTCATTTTTACCAACACATCAATTCCAAAGCCTCAAGGGATTTACTGGGACAAATTGTCTCCGGAGAAGCTGGGGCAAATTAAAATACTCAGAGAACTGAAGAGAAGGATTGAACAAGAGACTGGACAAAAATTGCCATGCGGTCCATCAGAGAAACTGCCTCCCACAGCGCGAAGAGGAGCTAACTTGGTAGGTGGTAAAAGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

405

Amino Acids

44.79

Weight (kDa)

8.66

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 5 - 41 2.1e-09 FtsJ-like methyltransferase
5-FTHF_cyc-lig PF01812 136 - 334 5.8e-35 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 402
AccBSI CCGCTC 1 cut(s) 328
AccII CGCG 1 cut(s) 1180
AccIII TCCGGA 3 cut(s) 184, 417, 1069
AciI CCGC 9 cut(s) 236, 263, 328, 448, 484, 510, 531, 745, 1149
AclWI GGATC 2 cut(s) 409, 803
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 2 cut(s) 188, 426
AcuI CTGAAG 2 cut(s) 8, 1127
AcyI GRCGYC 3 cut(s) 291, 342, 504
AfaI GTAC 2 cut(s) 700, 978
AfiI CCNNNNNNNGG 7 cut(s) 164, 351, 402, 651, 726, 741, 818
AgsI TTSAA 6 cut(s) 16, 172, 677, 860, 900, 1121
AhdI GACNNNNNGTC 1 cut(s) 1063
AjnI CCWGG 3 cut(s) 88, 812, 1005
AleI CACNNNNGTG 1 cut(s) 917
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 2 cut(s) 1071, 1121
AlwI GGATC 2 cut(s) 409, 803
Aor13HI TCCGGA 3 cut(s) 184, 417, 1069
AoxI GGCC 6 cut(s) 295, 321, 444, 470, 732, 746
ApeKI GCWGC 6 cut(s) 83, 311, 333, 512, 515, 871
ApoI RAATTY 2 cut(s) 188, 426
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 333, 506, 1180
AspS9I GGNCC 3 cut(s) 296, 445, 1151
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 4 cut(s) 218, 424, 470, 851
AvaII GGWCC 1 cut(s) 1151
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 826
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 6 cut(s) 70, 323, 345, 499, 502, 858
BccI CCATC 5 cut(s) 47, 231, 433, 611, 1162
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 3 cut(s) 90, 814, 1007
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 2 cut(s) 1071, 1121
BfaI CTAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 507
BisI GCNGC 8 cut(s) 84, 312, 329, 334, 513, 516, 746, 872
BlsI GCNGC 8 cut(s) 85, 313, 330, 335, 514, 517, 747, 873
BmcAI AGTACT 1 cut(s) 700
Bme1390I CCNGG 4 cut(s) 90, 575, 814, 1007
Bme18I GGWCC 1 cut(s) 1151
BmeRI GACNNNNNGTC 1 cut(s) 1063
BmgT120I GGNCC 3 cut(s) 296, 445, 1151
BmiI GGNNCC 4 cut(s) 298, 505, 796, 886
BmrFI CCNGG 4 cut(s) 90, 575, 814, 1007
BmrI ACTGGG 2 cut(s) 666, 1063
BmsI GCATC 2 cut(s) 495, 858
BmuI ACTGGG 2 cut(s) 666, 1063
BpiI GAAGAC 1 cut(s) 396
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 1026
BpuMI CCSGG 1 cut(s) 575
BsaHI GRCGYC 3 cut(s) 291, 342, 504
BsaJI CCNNGG 6 cut(s) 88, 345, 360, 467, 812, 1005
BsaWI WCCGGW 3 cut(s) 184, 417, 1069
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 238, 268
Bsc4I CCNNNNNNNGG 7 cut(s) 164, 351, 402, 651, 726, 741, 818
Bse1I ACTGG 6 cut(s) 378, 661, 905, 980, 1058, 1135
BseAI TCCGGA 3 cut(s) 184, 417, 1069
BseBI CCWGG 3 cut(s) 90, 814, 1007
BseDI CCNNGG 6 cut(s) 88, 345, 360, 467, 812, 1005
BseGI GGATG 2 cut(s) 486, 873
BseLI CCNNNNNNNGG 7 cut(s) 164, 351, 402, 651, 726, 741, 818
BseMII CTCAG 3 cut(s) 357, 599, 1111
BseNI ACTGG 6 cut(s) 378, 661, 905, 980, 1058, 1135
BseRI GAGGAG 4 cut(s) 383, 573, 627, 1200
BseXI GCAGC 6 cut(s) 70, 323, 345, 499, 502, 858
BseYI CCCAGC 1 cut(s) 1078
Bsh1236I CGCG 1 cut(s) 1180
BshFI GGCC 6 cut(s) 297, 323, 446, 472, 734, 748
BshNI GGYRCC 1 cut(s) 503
BsiHKAI GWGCWC 1 cut(s) 826
BsiSI CCGG 4 cut(s) 185, 418, 575, 1070
BslFI GGGAC 2 cut(s) 446, 1070
BslI CCNNNNNNNGG 7 cut(s) 164, 351, 402, 651, 726, 741, 818
BsmAI GTCTC 2 cut(s) 1071, 1121
BsmFI GGGAC 2 cut(s) 446, 1070
BsnI GGCC 6 cut(s) 297, 323, 446, 472, 734, 748
Bsp1286I GDGCHC 1 cut(s) 826
Bsp13I TCCGGA 3 cut(s) 184, 417, 1069
Bsp143I GATC 4 cut(s) 73, 414, 778, 808
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 9 cut(s) 236, 263, 328, 448, 484, 510, 531, 745, 1149
BspANI GGCC 6 cut(s) 297, 323, 446, 472, 734, 748
BspCNI CTCAG 3 cut(s) 358, 598, 1110
BspEI TCCGGA 3 cut(s) 184, 417, 1069
BspFNI CGCG 1 cut(s) 1180
BspLI GGNNCC 4 cut(s) 298, 505, 796, 886
BspPI GGATC 2 cut(s) 409, 803
BspT107I GGYRCC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 328
BsrI ACTGG 6 cut(s) 378, 661, 905, 980, 1058, 1135
BssECI CCNNGG 6 cut(s) 88, 345, 360, 467, 812, 1005
BssMI GATC 4 cut(s) 73, 414, 778, 808
BssNI GRCGYC 3 cut(s) 291, 342, 504
BssT1I CCWWGG 3 cut(s) 345, 360, 467
Bst2UI CCWGG 3 cut(s) 90, 814, 1007
Bst4CI ACNGT 3 cut(s) 560, 919, 929
Bst6I CTCTTC 4 cut(s) 276, 416, 1103, 1177
BstACI GRCGYC 3 cut(s) 291, 342, 504
BstC8I GCNNGC 3 cut(s) 132, 448, 715
BstDEI CTNAG 3 cut(s) 366, 585, 1097
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 486, 873
BstFNI CGCG 1 cut(s) 1180
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 3 cut(s) 333, 506, 1180
BstKTI GATC 4 cut(s) 76, 417, 781, 811
BstMAI GTCTC 2 cut(s) 1071, 1121
BstMBI GATC 4 cut(s) 73, 414, 778, 808
BstMWI GCNNNNNNNGC 4 cut(s) 136, 320, 371, 512
BstNI CCWGG 3 cut(s) 90, 814, 1007
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 4 cut(s) 88, 573, 812, 1005
BstUI CGCG 1 cut(s) 1180
BstV1I GCAGC 6 cut(s) 70, 323, 345, 499, 502, 858
BstV2I GAAGAC 1 cut(s) 396
BsuRI GGCC 6 cut(s) 297, 323, 446, 472, 734, 748
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 2 cut(s) 486, 873
BtsIMutI CAGTG 1 cut(s) 385
Cac8I GCNNGC 3 cut(s) 132, 448, 715
CfoI GCGC 3 cut(s) 333, 506, 1180
Cfr13I GGNCC 3 cut(s) 296, 445, 1151
CseI GACGC 2 cut(s) 317, 350
Csp6I GTAC 2 cut(s) 699, 977
CviAII CATG 8 cut(s) 65, 278, 361, 436, 880, 923, 971, 1146
CviQI GTAC 2 cut(s) 699, 977
DdeI CTNAG 3 cut(s) 366, 585, 1097
DinI GGCGCC 1 cut(s) 505
DpnI GATC 4 cut(s) 75, 416, 780, 810
DpnII GATC 4 cut(s) 73, 414, 778, 808
DriI GACNNNNNGTC 1 cut(s) 1063
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 4 cut(s) 276, 416, 1103, 1177
Eam1105I GACNNNNNGTC 1 cut(s) 1063
EarI CTCTTC 4 cut(s) 276, 416, 1103, 1177
Eco130I CCWWGG 3 cut(s) 345, 360, 467
Eco147I AGGCCT 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 1151
Eco57I CTGAAG 2 cut(s) 8, 1127
EcoRI GAATTC 1 cut(s) 188
EcoRII CCWGG 3 cut(s) 88, 812, 1005
EcoT14I CCWWGG 3 cut(s) 345, 360, 467
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
ErhI CCWWGG 3 cut(s) 345, 360, 467
FaeI CATG 8 cut(s) 68, 281, 364, 439, 883, 926, 974, 1149
FaqI GGGAC 2 cut(s) 446, 1070
FatI CATG 8 cut(s) 64, 277, 360, 435, 879, 922, 970, 1145
FauI CCCGC 4 cut(s) 243, 270, 455, 524
FauNDI CATATG 1 cut(s) 992
Fnu4HI GCNGC 8 cut(s) 84, 312, 329, 334, 513, 516, 746, 872
FokI GGATG 2 cut(s) 473, 880
Fsp4HI GCNGC 8 cut(s) 84, 312, 329, 334, 513, 516, 746, 872
FspBI CTAG 1 cut(s) 696
GlaI GCGC 3 cut(s) 332, 505, 1179
GluI GCNGC 8 cut(s) 84, 312, 329, 334, 513, 516, 746, 872
GsaI CCCAGC 1 cut(s) 1082
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 6 cut(s) 297, 323, 446, 472, 734, 748
HapII CCGG 4 cut(s) 185, 418, 575, 1070
HgaI GACGC 2 cut(s) 317, 350
HhaI GCGC 3 cut(s) 333, 506, 1180
Hin1I GRCGYC 3 cut(s) 291, 342, 504
Hin1II CATG 8 cut(s) 68, 281, 364, 439, 883, 926, 974, 1149
Hin6I GCGC 3 cut(s) 331, 504, 1178
HinP1I GCGC 3 cut(s) 331, 504, 1178
HincII GTYRAC 3 cut(s) 633, 690, 985
HindII GTYRAC 3 cut(s) 633, 690, 985
HindIII AAGCTT 3 cut(s) 17, 137, 399
HinfI GANTC 8 cut(s) 38, 100, 149, 168, 578, 677, 691, 896
HpaI GTTAAC 1 cut(s) 633
HpaII CCGG 4 cut(s) 185, 418, 575, 1070
HphI GGTGA 4 cut(s) 218, 424, 470, 851
Hpy166II GTNNAC 6 cut(s) 478, 571, 633, 650, 690, 985
Hpy188I TCNGA 7 cut(s) 27, 148, 588, 616, 682, 1100, 1159
Hpy188III TCNNGA 4 cut(s) 185, 418, 553, 1070
Hpy8I GTNNAC 6 cut(s) 478, 571, 633, 650, 690, 985
Hpy99I CGWCG 7 cut(s) 74, 293, 296, 308, 311, 344, 503
HpyAV CCTTC 2 cut(s) 310, 1107
HpyCH4III ACNGT 3 cut(s) 560, 919, 929
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 4 cut(s) 717, 851, 922, 996
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 320, 371, 512
HpyF3I CTNAG 3 cut(s) 366, 585, 1097
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 3 cut(s) 291, 342, 504
Hsp92II CATG 8 cut(s) 68, 281, 364, 439, 883, 926, 974, 1149
HspAI GCGC 3 cut(s) 331, 504, 1178
KasI GGCGCC 1 cut(s) 503
Kpn2I TCCGGA 3 cut(s) 184, 417, 1069
KspAI GTTAAC 1 cut(s) 633
Kzo9I GATC 4 cut(s) 73, 414, 778, 808
LmnI GCTCC 5 cut(s) 187, 221, 800, 884, 1187
Lsp1109I GCAGC 6 cut(s) 70, 323, 345, 499, 502, 858
LweI GCATC 2 cut(s) 495, 858
MaeI CTAG 1 cut(s) 696
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 382, 910
MalI GATC 4 cut(s) 75, 416, 780, 810
MbiI CCGCTC 1 cut(s) 328
MboI GATC 4 cut(s) 73, 414, 778, 808
MboII GAAGA 9 cut(s) 14, 40, 263, 266, 401, 433, 634, 1120, 1194
MfeI CAATTG 1 cut(s) 951
MhlI GDGCHC 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 734
MluNI TGGCCA 1 cut(s) 734
Mly113I GGCGCC 1 cut(s) 504
MlyI GAGTC 2 cut(s) 109, 685
MmeI TCCRAC 2 cut(s) 516, 1025
Mox20I TGGCCA 1 cut(s) 734
MroI TCCGGA 3 cut(s) 184, 417, 1069
MscI TGGCCA 1 cut(s) 734
MseI TTAA 2 cut(s) 632, 1089
MslI CAYNNNNRTG 1 cut(s) 917
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 4 cut(s) 185, 418, 575, 1070
MspR9I CCNGG 4 cut(s) 90, 575, 814, 1007
MunI CAATTG 1 cut(s) 951
MvaI CCWGG 3 cut(s) 90, 814, 1007
MvnI CGCG 1 cut(s) 1180
MwoI GCNNNNNNNGC 4 cut(s) 136, 320, 371, 512
NarI GGCGCC 1 cut(s) 504
NciI CCSGG 1 cut(s) 575
NcoI CCATGG 1 cut(s) 360
NdeI CATATG 1 cut(s) 992
NdeII GATC 4 cut(s) 73, 414, 778, 808
NlaIII CATG 8 cut(s) 68, 281, 364, 439, 883, 926, 974, 1149
NlaIV GGNNCC 4 cut(s) 298, 505, 796, 886
NmeAIII GCCGAG 2 cut(s) 331, 349
NmuCI GTSAC 1 cut(s) 910
NspI RCATGY 1 cut(s) 68
OliI CACNNNNGTG 1 cut(s) 917
PceI AGGCCT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 315
PfeI GAWTC 6 cut(s) 38, 149, 168, 578, 677, 896
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 8 cut(s) 85, 313, 330, 335, 514, 517, 747, 873
PleI GAGTC 2 cut(s) 108, 685
PluTI GGCGCC 1 cut(s) 507
PpsI GAGTC 2 cut(s) 108, 685
Psp6I CCWGG 3 cut(s) 88, 812, 1005
PspFI CCCAGC 1 cut(s) 1078
PspGI CCWGG 3 cut(s) 88, 812, 1005
PspN4I GGNNCC 4 cut(s) 298, 505, 796, 886
PspPI GGNCC 3 cut(s) 296, 445, 1151
RsaI GTAC 2 cut(s) 700, 978
RsaNI GTAC 2 cut(s) 699, 977
RseI CAYNNNNRTG 1 cut(s) 917
SaqAI TTAA 2 cut(s) 632, 1089
SatI GCNGC 8 cut(s) 84, 312, 329, 334, 513, 516, 746, 872
Sau3AI GATC 4 cut(s) 73, 414, 778, 808
Sau96I GGNCC 3 cut(s) 296, 445, 1151
ScaI AGTACT 1 cut(s) 700
SchI GAGTC 2 cut(s) 109, 685
ScrFI CCNGG 4 cut(s) 90, 575, 814, 1007
SduI GDGCHC 1 cut(s) 826
SfaNI GCATC 2 cut(s) 495, 858
SfoI GGCGCC 1 cut(s) 505
SinI GGWCC 1 cut(s) 1151
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 1 cut(s) 1041
SmoI CTYRAG 1 cut(s) 1041
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 9 cut(s) 236, 263, 328, 448, 484, 510, 531, 745, 1149
SspDI GGCGCC 1 cut(s) 503
SspMI CTAG 1 cut(s) 696
StuI AGGCCT 1 cut(s) 472
StyD4I CCNGG 4 cut(s) 88, 573, 812, 1005
StyI CCWWGG 3 cut(s) 345, 360, 467
TaaI ACNGT 3 cut(s) 560, 919, 929
TaiI ACGT 1 cut(s) 294
TaqI TCGA 5 cut(s) 51, 72, 303, 339, 826
TatI WGTACW 1 cut(s) 698
TauI GCSGC 2 cut(s) 331, 748
TfiI GAWTC 6 cut(s) 38, 149, 168, 578, 677, 896
Tru1I TTAA 2 cut(s) 632, 1089
Tru9I TTAA 2 cut(s) 632, 1089
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 910
TseI GCWGC 6 cut(s) 83, 311, 333, 512, 515, 871
Tsp45I GTSAC 1 cut(s) 910
TspDTI ATGAA 2 cut(s) 266, 719
TspGWI ACGGA 4 cut(s) 401, 536, 667, 787
TspRI CASTG 1 cut(s) 385
Van91I CCANNNNNTGG 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 1151
XapI RAATTY 2 cut(s) 188, 426
XceI RCATGY 1 cut(s) 68
XspI CTAG 1 cut(s) 696
ZraI GACGTC 1 cut(s) 292
ZrmI AGTACT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.