RLG00000012383

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000837
Physical Location & Seq
Reverse (-)
18131045 .. 18132277
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012383

Sequence Viewer

Length: 798 bp
ATGGTGAAGGCAATTAAAGATGCATGTGAAACATGGAGGTTCTTTCAGAAAGTTTACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTGAAGGTATTCGTAGATTTCACAAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATTCAGTCAACTTTTATTGCAGTGGAGATCTGAAATTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCTTGCAGAGCCGTAGATGATAAACGGGACTTTGAAGCACTGCCTCAAGTGTGCAGACGTGAAATAAGTGAGTATATTAAAAACTTAATTGAACTTCAAAATGATCAACTAGCACGCATGGGGTGCTTGAAAGCTTTCTCATTGTCATGCGACTATTATCCAATTTCCCCTAATCCCTCATTTCTTACCTTAGTCCTGCAAGACGAGATTGGAGGGCTCCAAGTTCTCAGTAAGGATCAATGGGTTGACGTGCTGCCAGTGGGTGGAGCATTTGTAGCAAATCTTGGTGACCTTATGCAGCTCGTTACTAATAACAAGTTCAAGAGTGAGGAACATAGAGTGCTAGCTACCCCGCTTGTCAAGCCACGAATATCAGTTGCATCTTTCTTTGCTCCTGTGGATAGCGACAAACGTGTCAAGCCATATGGGCCTATAAAGGAGCTTCTATCTGAGAACAATCCCCCAATATACAGGGACACCCTTTACGGAGAGTATACTGGGTATTACAAAGTTAATGGACAAAATGGCAGCTCTGCCCTGCCTAATTTCAGACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

266

Amino Acids

30.26

Weight (kDa)

7.57

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 2 - 85 1.9e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 138 - 211 2.9e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 1 cut(s) 731
AciI CCGC 1 cut(s) 590
AclWI GGATC 1 cut(s) 480
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 500
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 7 cut(s) 95, 160, 272, 329, 335, 367, 559
AjiI CACGTC 2 cut(s) 296, 487
AluBI AGCT 5 cut(s) 371, 538, 584, 679, 768
AluI AGCT 5 cut(s) 371, 538, 584, 679, 768
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 1 cut(s) 480
AoxI GGCC 1 cut(s) 665
ApeKI GCWGC 5 cut(s) 118, 215, 490, 535, 765
ApoI RAATTY 1 cut(s) 163
Asp700I GAANNNNTTC 2 cut(s) 99, 371
AspS9I GGNCC 1 cut(s) 665
AsuHPI GGTGA 2 cut(s) 16, 536
AsuNHI GCTAGC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 456
BauI CACGAG 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 5 cut(s) 130, 227, 477, 547, 777
BccI CCATC 1 cut(s) 130
BceAI ACGGC 1 cut(s) 233
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 2 cut(s) 347, 581
BfmI CTRYAG 1 cut(s) 123
BglI GCCNNNNNGGC 1 cut(s) 664
BglII AGATCT 1 cut(s) 190
BisI GCNGC 5 cut(s) 119, 216, 491, 536, 766
BlsI GCNGC 5 cut(s) 120, 217, 492, 537, 767
BmgBI CACGTC 2 cut(s) 296, 487
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 455
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 3 cut(s) 10, 78, 626
BmtI GCTAGC 1 cut(s) 584
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpuEI CTTGAG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 494, 739
BseDI CCNNGG 1 cut(s) 58
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMII CTCAG 2 cut(s) 478, 678
BseNI ACTGG 2 cut(s) 494, 739
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 5 cut(s) 130, 227, 477, 547, 777
BsgI GTGCAG 1 cut(s) 310
BshFI GGCC 1 cut(s) 667
BslFI GGGAC 2 cut(s) 278, 725
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 222
BsmFI GGGAC 2 cut(s) 278, 725
BsnI GGCC 1 cut(s) 667
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 3 cut(s) 190, 340, 472
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 1 cut(s) 590
BspANI GGCC 1 cut(s) 667
BspCNI CTCAG 2 cut(s) 477, 679
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 127
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 1 cut(s) 480
BsrI ACTGG 2 cut(s) 494, 739
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 190, 340, 472
BssNAI GTATAC 1 cut(s) 732
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 58
Bst1107I GTATAC 1 cut(s) 732
Bst2BI CACGAG 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 4 cut(s) 123, 204, 352, 582
BstDEI CTNAG 3 cut(s) 427, 464, 687
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 524
BstKTI GATC 3 cut(s) 193, 343, 475
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 3 cut(s) 190, 340, 472
BstMWI GCNNNNNNNGC 5 cut(s) 212, 360, 512, 598, 664
BstNSI RCATGY 1 cut(s) 27
BstPI GGTNACC 1 cut(s) 524
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 5 cut(s) 130, 227, 477, 547, 777
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BstZ17I GTATAC 1 cut(s) 732
BsuRI GGCC 1 cut(s) 667
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 2 cut(s) 296, 487
BtsI GCAGTG 2 cut(s) 190, 275
BtsIMutI CAGTG 3 cut(s) 190, 275, 501
Cac8I GCNNGC 4 cut(s) 123, 204, 352, 582
Cfr13I GGNCC 1 cut(s) 665
Csp6I GTAC 1 cut(s) 145
CviAII CATG 5 cut(s) 24, 33, 59, 355, 384
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 3 cut(s) 427, 464, 687
DpnI GATC 3 cut(s) 192, 342, 474
DpnII GATC 3 cut(s) 190, 340, 472
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 456
Eco91I GGTNACC 1 cut(s) 524
EcoO65I GGTNACC 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 5 cut(s) 27, 36, 62, 358, 387
FaqI GGGAC 2 cut(s) 278, 725
FatI CATG 5 cut(s) 23, 32, 58, 354, 383
FauI CCCGC 1 cut(s) 597
FauNDI CATATG 1 cut(s) 661
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 731
Fnu4HI GCNGC 5 cut(s) 119, 216, 491, 536, 766
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 5 cut(s) 119, 216, 491, 536, 766
FspBI CTAG 2 cut(s) 347, 581
GluI GCNGC 5 cut(s) 119, 216, 491, 536, 766
HaeIII GGCC 1 cut(s) 667
Hin1II CATG 5 cut(s) 27, 36, 62, 358, 387
HincII GTYRAC 2 cut(s) 172, 484
HindII GTYRAC 2 cut(s) 172, 484
HindIII AAGCTT 1 cut(s) 369
HphI GGTGA 2 cut(s) 16, 536
Hpy166II GTNNAC 4 cut(s) 55, 172, 484, 732
Hpy188I TCNGA 4 cut(s) 48, 195, 688, 788
Hpy188III TCNNGA 3 cut(s) 152, 160, 559
Hpy8I GTNNAC 4 cut(s) 55, 172, 484, 732
HpyAV CCTTC 1 cut(s) 89
HpyCH4IV ACGT 3 cut(s) 295, 486, 649
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 360, 512, 598, 664
HpyF3I CTNAG 3 cut(s) 427, 464, 687
HpySE526I ACGT 3 cut(s) 295, 486, 649
Hsp92II CATG 5 cut(s) 27, 36, 62, 358, 387
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 3 cut(s) 190, 340, 472
LmnI GCTCC 4 cut(s) 459, 503, 634, 676
LpnPI CCDG 7 cut(s) 135, 446, 507, 645, 694, 720, 788
Lsp1109I GCAGC 5 cut(s) 130, 227, 477, 547, 777
LweI GCATC 3 cut(s) 10, 78, 626
MaeI CTAG 2 cut(s) 347, 581
MaeII ACGT 3 cut(s) 295, 486, 649
MaeIII GTNAC 3 cut(s) 152, 524, 541
MalI GATC 3 cut(s) 192, 342, 474
MboI GATC 3 cut(s) 190, 340, 472
MboII GAAGA 1 cut(s) 140
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 6 cut(s) 12, 163, 197, 324, 399, 781
MnlI CCTC 6 cut(s) 30, 76, 291, 424, 443, 559
Mph1103I ATGCAT 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 99, 371
MseI TTAA 4 cut(s) 15, 315, 323, 750
MslI CAYNNNNRTG 1 cut(s) 382
MwoI GCNNNNNNNGC 5 cut(s) 212, 360, 512, 598, 664
NcoI CCATGG 1 cut(s) 58
NdeI CATATG 1 cut(s) 661
NdeII GATC 3 cut(s) 190, 340, 472
NheI GCTAGC 1 cut(s) 580
NlaIII CATG 5 cut(s) 27, 36, 62, 358, 387
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 2 cut(s) 152, 524
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 1 cut(s) 27
PdmI GAANNNNTTC 2 cut(s) 99, 371
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 5 cut(s) 120, 217, 492, 537, 767
PspEI GGTNACC 1 cut(s) 524
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 665
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 382
SaqAI TTAA 4 cut(s) 15, 315, 323, 750
SatI GCNGC 5 cut(s) 119, 216, 491, 536, 766
Sau3AI GATC 3 cut(s) 190, 340, 472
Sau96I GGNCC 1 cut(s) 665
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 3 cut(s) 10, 78, 626
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 6 cut(s) 12, 163, 197, 324, 399, 781
SsiI CCGC 1 cut(s) 590
SspMI CTAG 2 cut(s) 347, 581
StyI CCWWGG 1 cut(s) 58
TaiI ACGT 3 cut(s) 298, 489, 652
TasI AATT 6 cut(s) 12, 163, 197, 324, 399, 781
TatI WGTACW 1 cut(s) 144
Tru1I TTAA 4 cut(s) 15, 315, 323, 750
Tru9I TTAA 4 cut(s) 15, 315, 323, 750
TscAI CASTG 3 cut(s) 190, 282, 501
TseFI GTSAC 2 cut(s) 152, 524
TseI GCWGC 5 cut(s) 118, 215, 490, 535, 765
Tsp45I GTSAC 2 cut(s) 152, 524
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 3 cut(s) 190, 282, 501
Van91I CCANNNNNTGG 1 cut(s) 500
XapI RAATTY 1 cut(s) 163
XceI RCATGY 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 731
XmnI GAANNNNTTC 2 cut(s) 99, 371
XspI CTAG 2 cut(s) 347, 581
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.