Rh6CG322000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000837
Physical Location & Seq
Forward (+)
50834321 .. 50835555
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG322000.1

Sequence Viewer

Length: 693 bp
ATGGTGAAGGCAATTAAAGATGCATGTGAAACATGGAGGTTCTTTCAGATAGTTTACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGTATTCGTAGATTTCACGAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATTCAGCCAACTTTGATTGCAGTGGAGATCTGAAATTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCTTGTAGAGCCGTAGATGATAAATGGGACTTTGAAGCACTGCCTCAAGTGTGCAGACGTGAAATAAATCCCTCATTTCTTACCTTAGTCCTGCAAGACGAGATTGGAGGGCTCCAAGTTCTCAATAAGGATCAATGGGTTGACGTGTCGCCAGTGGATGGAGCATTTGTAGCAAATCTTGGTGACCTTATGCAGCTCATTACTAATAACAAGTTCAATAGTGTGGAACATAGAGTGCTAGCTACCCCGCTTGTCAAGCCACGAATATCAGTTGCATCTTTCTTTGCTCCTGCGGATAGCGACAAACGTGTCAAGCCATATGGGCCTATAAAGGAGCTTCTATCTGAGAACAATCCCCCAATATACAGGGACACCCTTTACGGAGAGTATACTGGGTATTACAGAGTTAATGGACAAAATGGCAGCTCTGCCCTGCCTAATTTCAGACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

230

Amino Acids

26.22

Weight (kDa)

5.85

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 2 - 84 5.6e-15 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 100 - 176 1.8e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 395
AccI GTMKAC 1 cut(s) 626
AciI CCGC 2 cut(s) 485, 530
AclWI GGATC 1 cut(s) 375
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 395
AflIII ACRYGT 2 cut(s) 381, 544
AgsI TTSAA 4 cut(s) 95, 160, 272, 454
AjiI CACGTC 2 cut(s) 296, 382
AluBI AGCT 4 cut(s) 433, 479, 574, 663
AluI AGCT 4 cut(s) 433, 479, 574, 663
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 1 cut(s) 375
AoxI GGCC 1 cut(s) 560
ApeKI GCWGC 4 cut(s) 118, 215, 430, 660
ApoI RAATTY 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 2 cut(s) 16, 431
AsuNHI GCTAGC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 351
BauI CACGAG 2 cut(s) 113, 150
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 4 cut(s) 130, 227, 442, 672
BccI CCATC 2 cut(s) 130, 389
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 1 cut(s) 476
BfmI CTRYAG 1 cut(s) 123
BglI GCCNNNNNGGC 1 cut(s) 559
BglII AGATCT 1 cut(s) 190
BisI GCNGC 4 cut(s) 119, 216, 431, 661
BlsI GCNGC 4 cut(s) 120, 217, 432, 662
BmgBI CACGTC 2 cut(s) 296, 382
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 350
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 3 cut(s) 10, 78, 521
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpuEI CTTGAG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 389, 634
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 2 cut(s) 389, 634
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 4 cut(s) 130, 227, 442, 672
BsgI GTGCAG 1 cut(s) 310
BshFI GGCC 1 cut(s) 562
BslFI GGGAC 2 cut(s) 278, 620
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 222
BsmFI GGGAC 2 cut(s) 278, 620
BsnI GGCC 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 2 cut(s) 190, 367
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 2 cut(s) 485, 530
BspANI GGCC 1 cut(s) 562
BspCNI CTCAG 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 350
BspMAI CTGCAG 1 cut(s) 127
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 1 cut(s) 375
BsrI ACTGG 2 cut(s) 389, 634
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 2 cut(s) 190, 367
BssNAI GTATAC 1 cut(s) 627
BssSI CACGAG 2 cut(s) 113, 150
BssT1I CCWWGG 1 cut(s) 58
Bst1107I GTATAC 1 cut(s) 627
Bst2BI CACGAG 2 cut(s) 113, 150
BstC8I GCNNGC 3 cut(s) 123, 204, 477
BstDEI CTNAG 2 cut(s) 322, 582
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 2 cut(s) 193, 370
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 2 cut(s) 190, 367
BstMWI GCNNNNNNNGC 4 cut(s) 212, 407, 493, 559
BstNSI RCATGY 1 cut(s) 27
BstPI GGTNACC 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 4 cut(s) 130, 227, 442, 672
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BstZ17I GTATAC 1 cut(s) 627
BsuRI GGCC 1 cut(s) 562
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 2 cut(s) 296, 382
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 2 cut(s) 190, 275
BtsIMutI CAGTG 3 cut(s) 190, 275, 396
Cac8I GCNNGC 3 cut(s) 123, 204, 477
Cfr13I GGNCC 1 cut(s) 560
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 24, 33, 59
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 322, 582
DpnI GATC 2 cut(s) 192, 369
DpnII GATC 2 cut(s) 190, 367
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 351
Eco91I GGTNACC 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 3 cut(s) 27, 36, 62
FaqI GGGAC 2 cut(s) 278, 620
FatI CATG 3 cut(s) 23, 32, 58
FauI CCCGC 1 cut(s) 492
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 4 cut(s) 119, 216, 431, 661
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 4 cut(s) 119, 216, 431, 661
FspBI CTAG 1 cut(s) 476
GluI GCNGC 4 cut(s) 119, 216, 431, 661
HaeIII GGCC 1 cut(s) 562
Hin1II CATG 3 cut(s) 27, 36, 62
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HphI GGTGA 2 cut(s) 16, 431
Hpy166II GTNNAC 3 cut(s) 55, 379, 627
Hpy188I TCNGA 4 cut(s) 48, 195, 583, 683
Hpy188III TCNNGA 3 cut(s) 113, 152, 160
Hpy8I GTNNAC 3 cut(s) 55, 379, 627
HpyCH4IV ACGT 3 cut(s) 295, 381, 544
HpyCH4V TGCA 9 cut(s) 23, 125, 183, 206, 291, 331, 430, 512, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 407, 493, 559
HpyF3I CTNAG 2 cut(s) 322, 582
HpySE526I ACGT 3 cut(s) 295, 381, 544
Hsp92II CATG 3 cut(s) 27, 36, 62
Kzo9I GATC 2 cut(s) 190, 367
LmnI GCTCC 4 cut(s) 354, 398, 529, 571
LpnPI CCDG 7 cut(s) 135, 341, 402, 540, 589, 615, 683
Lsp1109I GCAGC 4 cut(s) 130, 227, 442, 672
LweI GCATC 3 cut(s) 10, 78, 521
MaeI CTAG 1 cut(s) 476
MaeII ACGT 3 cut(s) 295, 381, 544
MaeIII GTNAC 2 cut(s) 152, 419
MalI GATC 2 cut(s) 192, 369
MboI GATC 2 cut(s) 190, 367
MboII GAAGA 1 cut(s) 140
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 4 cut(s) 12, 163, 197, 676
MnlI CCTC 5 cut(s) 30, 76, 291, 319, 338
Mph1103I ATGCAT 1 cut(s) 25
MseI TTAA 2 cut(s) 15, 645
MwoI GCNNNNNNNGC 4 cut(s) 212, 407, 493, 559
NcoI CCATGG 1 cut(s) 58
NdeI CATATG 1 cut(s) 556
NdeII GATC 2 cut(s) 190, 367
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 3 cut(s) 27, 36, 62
NlaIV GGNNCC 1 cut(s) 350
NmuCI GTSAC 2 cut(s) 152, 419
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 4 cut(s) 120, 217, 432, 662
PspEI GGTNACC 1 cut(s) 419
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 1 cut(s) 560
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 15, 645
SatI GCNGC 4 cut(s) 119, 216, 431, 661
Sau3AI GATC 2 cut(s) 190, 367
Sau96I GGNCC 1 cut(s) 560
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 3 cut(s) 10, 78, 521
SfcI CTRYAG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 4 cut(s) 12, 163, 197, 676
SsiI CCGC 2 cut(s) 485, 530
SspMI CTAG 1 cut(s) 476
StyI CCWWGG 1 cut(s) 58
TaiI ACGT 3 cut(s) 298, 384, 547
TasI AATT 4 cut(s) 12, 163, 197, 676
TatI WGTACW 1 cut(s) 144
Tru1I TTAA 2 cut(s) 15, 645
Tru9I TTAA 2 cut(s) 15, 645
TscAI CASTG 3 cut(s) 190, 282, 396
TseFI GTSAC 2 cut(s) 152, 419
TseI GCWGC 4 cut(s) 118, 215, 430, 660
Tsp45I GTSAC 2 cut(s) 152, 419
TspGWI ACGGA 1 cut(s) 633
TspRI CASTG 3 cut(s) 190, 282, 396
Van91I CCANNNNNTGG 1 cut(s) 395
XapI RAATTY 1 cut(s) 163
XceI RCATGY 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 626
XspI CTAG 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.