RchiOBHm_Chr6g0288671

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000837
Physical Location & Seq
Forward (+)
51869351 .. 51870155
805 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25899

Sequence Viewer

Length: 336 bp
ATGGTGAAGGCAATTAAAGATGCATGTGAAACATGGAGGTTCTTTCAGATAGTTTACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGTATTCGTAGATTTCACGAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATTCAGCCAACTTTGATTGCAGTGGAGATCTGAAATTGCGTGCAAAATCAGCAGCAGATTGGAGAGACACCTTATCTTGTAGAGCCGTAGATGATAAATGGGACTTTGAAGCACTGCCTCAAGTGTGCAGGTATTTCTATAGCTCCAGAGCCTATGCTATACTAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

111

Amino Acids

13.04

Weight (kDa)

5.67

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 2 - 84 7.5e-16 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 282
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 1 cut(s) 146
AgsI TTSAA 3 cut(s) 95, 160, 272
AluBI AGCT 1 cut(s) 306
AluI AGCT 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 118, 215
ApoI RAATTY 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 16
BauI CACGAG 2 cut(s) 113, 150
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 130, 227
BccI CCATC 1 cut(s) 130
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 123, 301
BfuAI ACCTGC 1 cut(s) 282
BglII AGATCT 1 cut(s) 190
BisI GCNGC 2 cut(s) 119, 216
BlsI GCNGC 2 cut(s) 120, 217
BmsI GCATC 2 cut(s) 10, 78
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 2 cut(s) 130, 227
BsgI GTGCAG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 278
Bsp143I GATC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 1 cut(s) 190
BssSI CACGAG 2 cut(s) 113, 150
BssT1I CCWWGG 1 cut(s) 58
Bst2BI CACGAG 2 cut(s) 113, 150
BstC8I GCNNGC 2 cut(s) 123, 204
BstDSI CCRYGG 1 cut(s) 58
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 27
BstSFI CTRYAG 2 cut(s) 123, 301
BstV1I GCAGC 2 cut(s) 130, 227
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 58
BtsI GCAGTG 2 cut(s) 190, 275
BtsIMutI CAGTG 2 cut(s) 190, 275
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 2 cut(s) 123, 204
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 24, 33, 59
CviJI RGCY 6 cut(s) 121, 170, 248, 306, 314, 331
CviKI_1 RGCY 6 cut(s) 121, 170, 248, 306, 314, 331
CviQI GTAC 1 cut(s) 145
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 3 cut(s) 27, 36, 62
FaiI YATR 7 cut(s) 25, 34, 60, 80, 303, 318, 323
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 23, 32, 58
Fnu4HI GCNGC 2 cut(s) 119, 216
Fsp4HI GCNGC 2 cut(s) 119, 216
FspBI CTAG 1 cut(s) 326
GluI GCNGC 2 cut(s) 119, 216
GsuI CTGGAG 1 cut(s) 292
Hin1II CATG 3 cut(s) 27, 36, 62
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 2 cut(s) 48, 195
Hpy188III TCNNGA 4 cut(s) 113, 152, 160, 309
Hpy8I GTNNAC 1 cut(s) 55
HpyCH4V TGCA 5 cut(s) 23, 125, 183, 206, 291
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
Hsp92II CATG 3 cut(s) 27, 36, 62
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 3 cut(s) 135, 277, 322
Lsp1109I GCAGC 2 cut(s) 130, 227
LweI GCATC 2 cut(s) 10, 78
MaeI CTAG 1 cut(s) 326
MaeIII GTNAC 1 cut(s) 152
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 1 cut(s) 140
MflI RGATCY 1 cut(s) 190
MluCI AATT 3 cut(s) 12, 163, 197
MnlI CCTC 3 cut(s) 30, 76, 291
Mph1103I ATGCAT 1 cut(s) 25
MseI TTAA 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 212
NcoI CCATGG 1 cut(s) 58
NdeII GATC 1 cut(s) 190
NlaIII CATG 3 cut(s) 27, 36, 62
NmuCI GTSAC 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 1 cut(s) 27
PkrI GCNGC 2 cut(s) 120, 217
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 2 cut(s) 119, 216
Sau3AI GATC 1 cut(s) 190
SetI ASST 5 cut(s) 41, 100, 236, 296, 308
SfaNI GCATC 2 cut(s) 10, 78
SfcI CTRYAG 2 cut(s) 123, 301
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 3 cut(s) 12, 163, 197
SspMI CTAG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 58
TasI AATT 3 cut(s) 12, 163, 197
TatI WGTACW 1 cut(s) 144
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 190, 282
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 2 cut(s) 118, 215
Tsp45I GTSAC 1 cut(s) 152
TspRI CASTG 2 cut(s) 190, 282
XapI RAATTY 1 cut(s) 163
XceI RCATGY 1 cut(s) 27
XspI CTAG 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.