Rh6BG314600

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000837
Physical Location & Seq
Forward (+)
54480246 .. 54484695
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG314600.1

Sequence Viewer

Length: 1269 bp
ATGTTATTGTATTTTGCCCTGGCCCGGCTGGCCACGCACCTTTGTAATCAAGTCATCTTTATCTTAGTCTTTGTCCGAATGTGTCAATTATTGATTGACAGTCAATATAGATTTAATAATCTTGGCCGACTACACCTATATATGAGGCAGCTACTCTCATCTTCAACTTGCAAACTTGTTCTTGGTTTACAATCTAAACACCAAAACATGGCAGCAGTTGTGGATTGTGATCAGCGTGAGAAGGAGCTGAAAGCATTCGACGAAAGCAAAGCTGGCGTCAAAGGAATTGCAGACGCTGGGGTGACCAAGCTCCCAAGCATGTTCATACACCCTCCGGAGAATCTACTAAACTCGGCATTTCCAGAAGATGATGAGCAACATCAGAAAATTTGCAACCTTCAAGTTCCGGTGATTGATCTCAGCAGTTTTGATAACAGCGAGAGGCGAAAAGAAATCGTCAATGACATAAACAAAGCAGCTGAGTCATGGGGCTTCTTTCAGTTGGTGAACCATGGAATTCCGCTTAATGTTATGGAGGGGATCCAGAAAAGTATCCGAGGGTTTCATGAGCTGCCCCAGGAGGAGAAGGTGAAGTGGTACTCGCGTGATTTTACGCAGAAGGTTAACTTCTTCAGCAATGGAGATTTGAAGGTGTCGACGCCAGCTGACTGGAGGGATACCTTGTCATGCAAGGTCCTGGAAGACCCAGAAAGCTTTGAAGCAATACCGCAAGTGTGCAGACGAGAAGTTAGTGAGTATATGAAACACATCGATGGAGTGCAAGAGAAGTTATCCGAGTTATTTTCAGATGCTTTAGGTCTCAATAGTGATTATCTTGCAAGCACAGGGTGCTTCAAATCGAGGTCACTGGCGTGCCACTATTTCCCGATTTGCCCTGAACCGCATTTGACTATGGGCGGAACCAAGCATTCGGACCTCTCGTTTCTTACCTTGCTCCTTCAAGACAGTGTTGGCGGCCTACAAGTTTTTCATCAAAATGTTTGGATTGATGTTCCCCCAGTGAAGGGAGCTTTGCTAGCAAATATTGCTGACATGATGCAGATCATTACAAATGGCAAGTTCAAGAGCGTGGAGCATAGAGTTTTGATGAAACCAACCGTCGAGCCTCGTATCTCAATTGCATGTTTTTTGGGTACGGATGACCTCCAAAAACCATACGGGCCGATAAAAGAACTTATATTGGAAAACAATCCACCGATTTATAGCGAAGTCAAATTCGGAGAATACATGAAAAAGTATAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

422

Amino Acids

47.94

Weight (kDa)

6.24

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 135 - 235 3.3e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 289 - 385 3.7e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 208
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 604
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 5 cut(s) 521, 728, 902, 918, 975
AclWI GGATC 2 cut(s) 535, 548
AcoI YGGCCR 2 cut(s) 30, 124
AcsI RAATTY 3 cut(s) 387, 516, 1235
AcuI CTGAAG 1 cut(s) 616
AcyI GRCGYC 2 cut(s) 276, 659
AdeI CACNNNGTG 1 cut(s) 849
AfaI GTAC 2 cut(s) 599, 1156
AfiI CCNNNNNNNGG 4 cut(s) 24, 208, 1024, 1025
AgsI TTSAA 7 cut(s) 165, 401, 649, 719, 856, 962, 1084
AjnI CCWGG 3 cut(s) 18, 576, 696
AleI CACNNNNGTG 1 cut(s) 871
AluBI AGCT 9 cut(s) 151, 247, 272, 310, 479, 571, 665, 714, 1031
AluI AGCT 9 cut(s) 151, 247, 272, 310, 479, 571, 665, 714, 1031
Alw26I GTCTC 1 cut(s) 824
AlwI GGATC 2 cut(s) 535, 548
AlwNI CAGNNNCTG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 5 cut(s) 21, 30, 124, 976, 1181
ApeKI GCWGC 4 cut(s) 148, 212, 476, 571
ApoI RAATTY 3 cut(s) 387, 516, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
Asp700I GAANNNNTTC 1 cut(s) 254
AspS9I GGNCC 4 cut(s) 22, 694, 934, 1181
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 4 cut(s) 313, 421, 517, 601
AsuNHI GCTAGC 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 694, 934
BaeI ACNNNNGTAYC 2 cut(s) 589, 622
BalI TGGCCA 1 cut(s) 32
BamHI GGATCC 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 708
BbvI GCAGC 4 cut(s) 160, 224, 488, 558
BccI CCATC 1 cut(s) 767
BciT130I CCWGG 3 cut(s) 20, 578, 698
BciVI GTATCC 2 cut(s) 563, 670
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 824
BfaI CTAG 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 563, 670
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 5 cut(s) 149, 213, 477, 572, 976
BlsI GCNGC 5 cut(s) 150, 214, 478, 573, 977
Bme1390I CCNGG 4 cut(s) 20, 25, 578, 698
Bme18I GGWCC 2 cut(s) 694, 934
BmgT120I GGNCC 4 cut(s) 22, 694, 934, 1181
BmiI GGNNCC 2 cut(s) 542, 922
BmrFI CCNGG 4 cut(s) 20, 25, 578, 698
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 2 cut(s) 799, 1047
BmtI GCTAGC 1 cut(s) 1040
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 691
BpuMI CCSGG 1 cut(s) 25
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 2 cut(s) 228, 1061
BsaHI GRCGYC 2 cut(s) 276, 659
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 4 cut(s) 18, 511, 556, 576
BsaWI WCCGGW 2 cut(s) 334, 406
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 208, 1024, 1025
Bse1I ACTGG 3 cut(s) 674, 873, 1019
Bse3DI GCAATG 1 cut(s) 643
Bse8I GATNNNNATC 2 cut(s) 228, 1061
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 3 cut(s) 20, 578, 698
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 4 cut(s) 18, 511, 556, 576
BseGI GGATG 1 cut(s) 1165
BseJI GATNNNNATC 2 cut(s) 228, 1061
BseLI CCNNNNNNNGG 4 cut(s) 24, 208, 1024, 1025
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 2 cut(s) 433, 471
BseNI ACTGG 3 cut(s) 674, 873, 1019
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 4 cut(s) 160, 224, 488, 558
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 604
BshFI GGCC 5 cut(s) 23, 32, 126, 978, 1183
BshVI ATCGAT 1 cut(s) 771
BsiSI CCGG 3 cut(s) 25, 335, 407
BslI CCNNNNNNNGG 4 cut(s) 24, 208, 1024, 1025
BsmAI GTCTC 1 cut(s) 824
BsmI GAATGC 2 cut(s) 254, 928
BsnI GGCC 5 cut(s) 23, 32, 126, 978, 1183
Bso31I GGTCTC 1 cut(s) 824
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 4 cut(s) 229, 415, 540, 1062
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 5 cut(s) 521, 728, 902, 918, 975
BspANI GGCC 5 cut(s) 23, 32, 126, 978, 1183
BspCNI CTCAG 2 cut(s) 432, 472
BspDI ATCGAT 1 cut(s) 771
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 604
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 2 cut(s) 542, 922
BspOI GCTAGC 1 cut(s) 1040
BspPI GGATC 2 cut(s) 535, 548
BspTNI GGTCTC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 643
BsrI ACTGG 3 cut(s) 674, 873, 1019
BssECI CCNNGG 4 cut(s) 18, 511, 556, 576
BssMI GATC 4 cut(s) 229, 415, 540, 1062
BssNI GRCGYC 2 cut(s) 276, 659
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 3 cut(s) 20, 578, 698
Bst4CI ACNGT 3 cut(s) 101, 968, 1120
BstACI GRCGYC 2 cut(s) 276, 659
BstAPI GCANNNNNTGC 2 cut(s) 849, 1046
BstC8I GCNNGC 6 cut(s) 30, 274, 663, 841, 874, 1038
BstDEI CTNAG 3 cut(s) 64, 419, 480
BstDSI CCRYGG 1 cut(s) 511
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 1 cut(s) 1165
BstFNI CGCG 1 cut(s) 604
BstKTI GATC 4 cut(s) 232, 418, 543, 1065
BstMAI GTCTC 1 cut(s) 824
BstMBI GATC 4 cut(s) 229, 415, 540, 1062
BstMWI GCNNNNNNNGC 6 cut(s) 29, 34, 273, 849, 1037, 1046
BstNI CCWGG 3 cut(s) 20, 578, 698
BstNSI RCATGY 2 cut(s) 322, 1146
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 4 cut(s) 18, 23, 576, 696
BstUI CGCG 1 cut(s) 604
BstV1I GCAGC 4 cut(s) 160, 224, 488, 558
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 540
BstXI CCANNNNNNTGG 1 cut(s) 669
BstYI RGATCY 1 cut(s) 540
Bsu15I ATCGAT 1 cut(s) 771
BsuI GTATCC 2 cut(s) 563, 670
BsuRI GGCC 5 cut(s) 23, 32, 126, 978, 1183
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 1 cut(s) 1165
BtsIMutI CAGTG 3 cut(s) 866, 973, 1026
Cac8I GCNNGC 6 cut(s) 30, 274, 663, 841, 874, 1038
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 565
Cfr13I GGNCC 4 cut(s) 22, 694, 934, 1181
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 3 cut(s) 265, 302, 667
Csp6I GTAC 2 cut(s) 598, 1155
CviAII CATG 9 cut(s) 208, 319, 486, 512, 566, 687, 1054, 1143, 1249
CviQI GTAC 2 cut(s) 598, 1155
DdeI CTNAG 3 cut(s) 64, 419, 480
DpnI GATC 4 cut(s) 231, 417, 542, 1064
DpnII GATC 4 cut(s) 229, 415, 540, 1062
DraIII CACNNNGTG 1 cut(s) 849
EaeI YGGCCR 2 cut(s) 30, 124
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 824
Eco47I GGWCC 2 cut(s) 694, 934
Eco57I CTGAAG 1 cut(s) 616
Eco91I GGTNACC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 694
EcoO65I GGTNACC 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 516
EcoRII CCWGG 3 cut(s) 18, 576, 696
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 9 cut(s) 211, 322, 489, 515, 569, 690, 1057, 1146, 1252
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FatI CATG 9 cut(s) 207, 318, 485, 511, 565, 686, 1053, 1142, 1248
FbaI TGATCA 1 cut(s) 229
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 5 cut(s) 149, 213, 477, 572, 976
FokI GGATG 1 cut(s) 1172
Fsp4HI GCNGC 5 cut(s) 149, 213, 477, 572, 976
FspBI CTAG 1 cut(s) 1037
GluI GCNGC 5 cut(s) 149, 213, 477, 572, 976
GsaI CCCAGC 1 cut(s) 300
GsuI CTGGAG 1 cut(s) 691
HaeIII GGCC 5 cut(s) 23, 32, 126, 978, 1183
HapII CCGG 3 cut(s) 25, 335, 407
HgaI GACGC 3 cut(s) 265, 302, 667
Hin1I GRCGYC 2 cut(s) 276, 659
Hin1II CATG 9 cut(s) 211, 322, 489, 515, 569, 690, 1057, 1146, 1252
HincII GTYRAC 2 cut(s) 625, 657
HindII GTYRAC 2 cut(s) 625, 657
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 2 cut(s) 340, 482
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 3 cut(s) 25, 335, 407
HphI GGTGA 4 cut(s) 313, 421, 517, 601
Hpy166II GTNNAC 4 cut(s) 188, 508, 625, 657
Hpy188I TCNGA 7 cut(s) 77, 384, 557, 796, 808, 934, 1241
Hpy188III TCNNGA 7 cut(s) 335, 362, 544, 566, 886, 962, 1084
Hpy8I GTNNAC 4 cut(s) 188, 508, 625, 657
Hpy99I CGWCG 3 cut(s) 263, 661, 1124
HpyAV CCTTC 7 cut(s) 235, 407, 580, 613, 643, 968, 1018
HpyCH4III ACNGT 3 cut(s) 101, 968, 1120
HpyCH4V TGCA 9 cut(s) 171, 290, 393, 690, 738, 781, 839, 1060, 1142
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 34, 273, 849, 1037, 1046
HpyF3I CTNAG 3 cut(s) 64, 419, 480
Hsp92I GRCGYC 2 cut(s) 276, 659
Hsp92II CATG 9 cut(s) 211, 322, 489, 515, 569, 690, 1057, 1146, 1252
Kpn2I TCCGGA 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 229
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 4 cut(s) 229, 415, 540, 1062
LmnI GCTCC 5 cut(s) 244, 315, 960, 1028, 1093
Lsp1109I GCAGC 4 cut(s) 160, 224, 488, 558
LweI GCATC 2 cut(s) 799, 1047
MaeI CTAG 1 cut(s) 1037
MaeIII GTNAC 2 cut(s) 301, 864
MalI GATC 4 cut(s) 231, 417, 542, 1064
MboI GATC 4 cut(s) 229, 415, 540, 1062
MboII GAAGA 4 cut(s) 153, 377, 622, 713
MfeI CAATTG 1 cut(s) 1137
MflI RGATCY 1 cut(s) 540
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 6 cut(s) 86, 285, 387, 516, 1137, 1235
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 1 cut(s) 491
Mox20I TGGCCA 1 cut(s) 32
MroI TCCGGA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 32
MseI TTAA 3 cut(s) 114, 525, 624
MslI CAYNNNNRTG 3 cut(s) 771, 871, 996
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 2 cut(s) 479, 665
MspI CCGG 3 cut(s) 25, 335, 407
MspR9I CCNGG 4 cut(s) 20, 25, 578, 698
MunI CAATTG 1 cut(s) 1137
Mva1269I GAATGC 2 cut(s) 254, 928
MvaI CCWGG 3 cut(s) 20, 578, 698
MvnI CGCG 1 cut(s) 604
MwoI GCNNNNNNNGC 6 cut(s) 29, 34, 273, 849, 1037, 1046
NciI CCSGG 1 cut(s) 25
NcoI CCATGG 1 cut(s) 511
NdeII GATC 4 cut(s) 229, 415, 540, 1062
NheI GCTAGC 1 cut(s) 1036
NlaIII CATG 9 cut(s) 211, 322, 489, 515, 569, 690, 1057, 1146, 1252
NlaIV GGNNCC 2 cut(s) 542, 922
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 2 cut(s) 301, 864
NspI RCATGY 2 cut(s) 322, 1146
OliI CACNNNNGTG 1 cut(s) 871
PagI TCATGA 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 938
PctI GAATGC 2 cut(s) 254, 928
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 340
PflMI CCANNNNNTGG 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 696
PkrI GCNGC 5 cut(s) 150, 214, 478, 573, 977
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PpuMI RGGWCCY 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 3 cut(s) 18, 576, 696
PspEI GGTNACC 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 3 cut(s) 18, 576, 696
PspN4I GGNNCC 2 cut(s) 542, 922
PspPI GGNCC 4 cut(s) 22, 694, 934, 1181
PspPPI RGGWCCY 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 540
PvuII CAGCTG 2 cut(s) 479, 665
RsaI GTAC 2 cut(s) 599, 1156
RsaNI GTAC 2 cut(s) 598, 1155
RseI CAYNNNNRTG 3 cut(s) 771, 871, 996
SalI GTCGAC 1 cut(s) 655
SaqAI TTAA 3 cut(s) 114, 525, 624
SatI GCNGC 5 cut(s) 149, 213, 477, 572, 976
Sau3AI GATC 4 cut(s) 229, 415, 540, 1062
Sau96I GGNCC 4 cut(s) 22, 694, 934, 1181
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 4 cut(s) 20, 25, 578, 698
SfaNI GCATC 2 cut(s) 799, 1047
SfiI GGCCNNNNNGGCC 1 cut(s) 29
SinI GGWCC 2 cut(s) 694, 934
SmiMI CAYNNNNRTG 3 cut(s) 771, 871, 996
Sse9I AATT 6 cut(s) 86, 285, 387, 516, 1137, 1235
SsiI CCGC 5 cut(s) 521, 728, 902, 918, 975
SspI AATATT 1 cut(s) 1045
SspMI CTAG 1 cut(s) 1037
StyD4I CCNGG 4 cut(s) 18, 23, 576, 696
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 101, 968, 1120
TaqI TCGA 5 cut(s) 258, 656, 771, 860, 1122
TasI AATT 6 cut(s) 86, 285, 387, 516, 1137, 1235
TauI GCSGC 1 cut(s) 978
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 3 cut(s) 114, 525, 624
Tru9I TTAA 3 cut(s) 114, 525, 624
TscAI CASTG 3 cut(s) 873, 973, 1026
TseFI GTSAC 2 cut(s) 301, 864
TseI GCWGC 4 cut(s) 148, 212, 476, 571
Tsp45I GTSAC 2 cut(s) 301, 864
TspDTI ATGAA 6 cut(s) 313, 554, 776, 980, 1124, 1265
TspGWI ACGGA 1 cut(s) 1172
TspRI CASTG 3 cut(s) 873, 973, 1026
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 694, 934
XapI RAATTY 3 cut(s) 387, 516, 1235
XceI RCATGY 2 cut(s) 322, 1146
XmiI GTMKAC 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.