Rw6G026710

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000837
Physical Location & Seq
Reverse (-)
50199147 .. 50200645
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026710.1

Sequence Viewer

Length: 1011 bp
ATGGCAGCAGTTGTGGATTGTCATCAGCATGAGAAGGAGCTCAAAGCATTCGATGAAAGCAAAGCTGGCGTCAAAGGACTTGCAGACGCTGGGGTAACCAAGCTCCCAACCATGTTCATACACCCTCCGGAGAATCTACTAAACTCGGCATTCCCAGAAGATGATGAGCAACATCAGAAAATTCGCAACCTTCAAGTTCCGGTGATTGATCTCAGAGGTTTTGATAACAGCGAGAGGCGAAAAGAAATCGTCAATGACATAAACAAAGCAGCTCAGTCATGGGGCTTCTTTCAGTTGGTGAACCATGGAATTCCGCTTAATGTTATGGAGGGGATCCAGAAAAGTATCCGAGGGTTTCATGAGCTGCCCCAGGAGGAGAAGGTGAAGTGGTACTCGCGTGATTTTACGCAGAAGGTTAACTTCTTCAGCAATGGAGATTTGAAGGTGTCGACGCCAGCTGACTGGAGGGATACCTTGTCATGCAAGGTCCTGGAAGAGCCAGAAAGCTTTGAAGCAATACCGCAAGTGTGCAGACGAGAAGTTAGTGAGTATATGAAACACATCGATGGAGTGCAACAGAAGTTATCCGAGTTATTTTCAGAAGCTTTAGGTCTCAATAGTGATTATCTTGCAAGCACAGGGTGCTTCAAATCGAGGTCACTGGCGTGCCATTATTTCCCGATTTGCCCTGAACCGCATCTGACTATGGGCGGAACCAAGCATTCGGACCTCTCGTTTCTCACCTTGCTCCTTCAAGACAGTGTTGGCGGCCTACAAGTTTTTCATCAAAATGTTTGGATTGATATCATTACAAATGGCAAGTTCAAGAGCGTGGAGCATAGAGTTTTGATGAAACAAACCGTTGAGCCTCGTATCTCAATTGCATGTTTTTTGGGTACGGATAACCTCCAAAAACCATACGGGCCAATAAAAGAACTTATATCAGAAAACAATCCACCAATTTATAGCGAAGTCAAATTCGGAGAATACATGAAAAAGTATAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

336

Amino Acids

38.24

Weight (kDa)

6.08

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 66 - 167 7e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 220 - 299 6.3e-17 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 449
AccII CGCG 1 cut(s) 397
AccIII TCCGGA 1 cut(s) 127
AciI CCGC 5 cut(s) 314, 521, 695, 711, 768
AclWI GGATC 2 cut(s) 328, 341
AcsI RAATTY 3 cut(s) 180, 309, 977
AcuI CTGAAG 1 cut(s) 409
AcyI GRCGYC 2 cut(s) 69, 452
AdeI CACNNNGTG 1 cut(s) 642
AfaI GTAC 2 cut(s) 392, 898
AgsI TTSAA 6 cut(s) 194, 442, 512, 649, 755, 826
AjnI CCWGG 2 cut(s) 369, 489
AleI CACNNNNGTG 1 cut(s) 664
AluBI AGCT 8 cut(s) 40, 65, 103, 272, 364, 458, 507, 605
AluI AGCT 8 cut(s) 40, 65, 103, 272, 364, 458, 507, 605
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 2 cut(s) 328, 341
AlwNI CAGNNNCTG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 2 cut(s) 769, 923
ApeKI GCWGC 3 cut(s) 5, 269, 364
ApoI RAATTY 3 cut(s) 180, 309, 977
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AspS9I GGNCC 3 cut(s) 487, 727, 923
AsuHPI GGTGA 4 cut(s) 214, 310, 394, 733
AvaII GGWCC 2 cut(s) 487, 727
BaeI ACNNNNGTAYC 2 cut(s) 382, 415
BamHI GGATCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 3 cut(s) 17, 281, 351
BccI CCATC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 371, 491
BciVI GTATCC 2 cut(s) 356, 463
BcoDI GTCTC 1 cut(s) 617
BfuI GTATCC 2 cut(s) 356, 463
BisI GCNGC 4 cut(s) 6, 270, 365, 769
BlsI GCNGC 4 cut(s) 7, 271, 366, 770
Bme1390I CCNGG 2 cut(s) 371, 491
Bme18I GGWCC 2 cut(s) 487, 727
BmgT120I GGNCC 3 cut(s) 487, 727, 923
BmiI GGNNCC 2 cut(s) 335, 715
BmrFI CCNGG 2 cut(s) 371, 491
BmsI GCATC 1 cut(s) 706
BpmI CTGGAG 1 cut(s) 484
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 2 cut(s) 21, 803
BsaHI GRCGYC 2 cut(s) 69, 452
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 304, 349, 369
BsaWI WCCGGW 2 cut(s) 127, 199
Bse1I ACTGG 2 cut(s) 467, 666
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 2 cut(s) 21, 803
BseAI TCCGGA 1 cut(s) 127
BseBI CCWGG 2 cut(s) 371, 491
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 304, 349, 369
BseJI GATNNNNATC 2 cut(s) 21, 803
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 2 cut(s) 226, 287
BseNI ACTGG 2 cut(s) 467, 666
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 3 cut(s) 17, 281, 351
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 771, 925
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 2 cut(s) 128, 200
BsmAI GTCTC 1 cut(s) 617
BsmI GAATGC 3 cut(s) 47, 149, 721
BsnI GGCC 2 cut(s) 771, 925
Bso31I GGTCTC 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 2 cut(s) 208, 333
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 5 cut(s) 314, 521, 695, 711, 768
BspANI GGCC 2 cut(s) 771, 925
BspCNI CTCAG 2 cut(s) 225, 286
BspDI ATCGAT 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 127
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 335, 715
BspPI GGATC 2 cut(s) 328, 341
BspQI GCTCTTC 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 2 cut(s) 467, 666
BssECI CCNNGG 3 cut(s) 304, 349, 369
BssMI GATC 2 cut(s) 208, 333
BssNI GRCGYC 2 cut(s) 69, 452
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 2 cut(s) 371, 491
Bst4CI ACNGT 2 cut(s) 761, 862
Bst6I CTCTTC 1 cut(s) 489
BstACI GRCGYC 2 cut(s) 69, 452
BstAPI GCANNNNNTGC 1 cut(s) 642
BstC8I GCNNGC 4 cut(s) 67, 456, 634, 667
BstDEI CTNAG 2 cut(s) 212, 273
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 94
BstFNI CGCG 1 cut(s) 397
BstKTI GATC 2 cut(s) 211, 336
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 208, 333
BstMWI GCNNNNNNNGC 2 cut(s) 66, 642
BstNI CCWGG 2 cut(s) 371, 491
BstNSI RCATGY 1 cut(s) 888
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 369, 489
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 17, 281, 351
BstX2I RGATCY 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 462
BstYI RGATCY 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 2 cut(s) 356, 463
BsuRI GGCC 2 cut(s) 771, 925
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 659, 766
Cac8I GCNNGC 4 cut(s) 67, 456, 634, 667
CaiI CAGNNNCTG 1 cut(s) 89
CciI TCATGA 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 487, 727, 923
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 3 cut(s) 58, 95, 460
Csp6I GTAC 2 cut(s) 391, 897
CviAII CATG 8 cut(s) 29, 112, 279, 305, 359, 480, 885, 991
CviQI GTAC 2 cut(s) 391, 897
DdeI CTNAG 2 cut(s) 212, 273
DpnI GATC 2 cut(s) 210, 335
DpnII GATC 2 cut(s) 208, 333
DraIII CACNNNGTG 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 617
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 2 cut(s) 487, 727
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 409
Eco91I GGTNACC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 487
EcoO65I GGTNACC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 369, 489
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 8 cut(s) 32, 115, 282, 308, 362, 483, 888, 994
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FatI CATG 8 cut(s) 28, 111, 278, 304, 358, 479, 884, 990
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 4 cut(s) 6, 270, 365, 769
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 6, 270, 365, 769
GluI GCNGC 4 cut(s) 6, 270, 365, 769
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 2 cut(s) 771, 925
HapII CCGG 2 cut(s) 128, 200
HgaI GACGC 3 cut(s) 58, 95, 460
Hin1I GRCGYC 2 cut(s) 69, 452
Hin1II CATG 8 cut(s) 32, 115, 282, 308, 362, 483, 888, 994
HincII GTYRAC 2 cut(s) 418, 450
HindII GTYRAC 2 cut(s) 418, 450
HindIII AAGCTT 2 cut(s) 505, 603
HinfI GANTC 1 cut(s) 133
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 2 cut(s) 128, 200
HphI GGTGA 4 cut(s) 214, 310, 394, 733
Hpy166II GTNNAC 3 cut(s) 301, 418, 450
Hpy188I TCNGA 9 cut(s) 177, 215, 350, 589, 601, 702, 727, 946, 983
Hpy188III TCNNGA 6 cut(s) 128, 337, 359, 679, 755, 826
Hpy8I GTNNAC 3 cut(s) 301, 418, 450
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 6 cut(s) 28, 200, 373, 406, 436, 761
HpyCH4III ACNGT 2 cut(s) 761, 862
HpyCH4V TGCA 6 cut(s) 83, 483, 531, 574, 632, 884
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 642
HpyF3I CTNAG 2 cut(s) 212, 273
Hsp92I GRCGYC 2 cut(s) 69, 452
Hsp92II CATG 8 cut(s) 32, 115, 282, 308, 362, 483, 888, 994
Kpn2I TCCGGA 1 cut(s) 127
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 208, 333
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 4 cut(s) 37, 108, 753, 835
Lsp1109I GCAGC 3 cut(s) 17, 281, 351
LweI GCATC 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 94, 657
MalI GATC 2 cut(s) 210, 335
MboI GATC 2 cut(s) 208, 333
MboII GAAGA 3 cut(s) 170, 415, 506
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 5 cut(s) 180, 309, 879, 960, 977
MroI TCCGGA 1 cut(s) 127
MseI TTAA 2 cut(s) 318, 417
MslI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 2 cut(s) 128, 200
MspR9I CCNGG 2 cut(s) 371, 491
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 3 cut(s) 47, 149, 721
MvaI CCWGG 2 cut(s) 371, 491
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 2 cut(s) 66, 642
NcoI CCATGG 1 cut(s) 304
NdeII GATC 2 cut(s) 208, 333
NlaIII CATG 8 cut(s) 32, 115, 282, 308, 362, 483, 888, 994
NlaIV GGNNCC 2 cut(s) 335, 715
NmeAIII GCCGAG 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 657
NspI RCATGY 1 cut(s) 888
OliI CACNNNNGTG 1 cut(s) 664
PagI TCATGA 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 731
PctI GAATGC 3 cut(s) 47, 149, 721
PfeI GAWTC 1 cut(s) 133
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 4 cut(s) 7, 271, 366, 770
PpuMI RGGWCCY 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 369, 489
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 369, 489
PspN4I GGNNCC 2 cut(s) 335, 715
PspPI GGNCC 3 cut(s) 487, 727, 923
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 2 cut(s) 392, 898
RsaNI GTAC 2 cut(s) 391, 897
RseI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
SacI GAGCTC 1 cut(s) 42
SalI GTCGAC 1 cut(s) 448
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 2 cut(s) 318, 417
SatI GCNGC 4 cut(s) 6, 270, 365, 769
Sau3AI GATC 2 cut(s) 208, 333
Sau96I GGNCC 3 cut(s) 487, 727, 923
ScrFI CCNGG 2 cut(s) 371, 491
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 1 cut(s) 706
SinI GGWCC 2 cut(s) 487, 727
SmiMI CAYNNNNRTG 4 cut(s) 27, 564, 664, 789
Sse9I AATT 5 cut(s) 180, 309, 879, 960, 977
SsiI CCGC 5 cut(s) 314, 521, 695, 711, 768
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 369, 489
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 761, 862
TaqI TCGA 4 cut(s) 51, 449, 564, 653
TasI AATT 5 cut(s) 180, 309, 879, 960, 977
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 2 cut(s) 318, 417
Tru9I TTAA 2 cut(s) 318, 417
TscAI CASTG 2 cut(s) 666, 766
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 3 cut(s) 5, 269, 364
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 7 cut(s) 69, 106, 347, 569, 773, 866, 1007
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 2 cut(s) 666, 766
VpaK11BI GGWCC 2 cut(s) 487, 727
XapI RAATTY 3 cut(s) 180, 309, 977
XceI RCATGY 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.