MD05G1200300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000837
Physical Location & Seq
Reverse (-)
32772373 .. 32773630
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1200300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGCCTGGGTTGGATCATGAGCTGGAGGAAGAAATGAAAAAATTCGACGAAACAAAAGCTGGTGTGAAAGGCCTTGTAGATTCGGGGGTGACCAAGCTCCCAAGAATGTTCAAACACCCTCCAGAGGATCTACCGTCCCCTAACCATAACAATGGCATTAGCAACCGCCAAGTTCCTGTCATTGATCTCGAAGATGTCGAGGACAGCGAAATGCGGAGCAAAATCATCAATGACCTCAGAAAGGCAGCTGAGGAATGGGGTTTCTTTCAGATTGTGAACCATTCAGTTCCACTTGGTGTGATGGATGATGTGCTGAAGGGCATTCGACGATTTCATGAGCAGCCCCAGGAAGCCAAGGAGGTGTGGTATTCACGCGATTTCACGAAGAAGGTCAACTTCGTTAGCAATGCAGAATTCAAGGTGTCTCTTCCAGCTTGTTGGAGAGACACATTATCTTGCAAGGTCCTAGAAGATGAGCATAACTTCGAGGCAATCCCTGAAATCTGCAGGGCGGAAATAAAAGAGTATATGAAATACCTTGTTCAAGTGAAGGATAAGCTTTCCAAATTAATTTCGGAATCTTTAGGTCTAAGCAGCGATTACCTTGACAACCTAAGGTGCTTCGAATCGAGGTCATTGGCATGCCACTATTATCCAGTTTGCCCAGAGCCCCATTTGACTCTCGGCGGACACAAGCATTCTGACCTCGGCTTCATCACCTTGCTCTTACGAGCTAGTGAAGGCCTACAGGTACTTCATCAAAATGTTTGGATTGACATTCACCCAGTGGAGGGAGCTTTGCTTGCCAATGTATCTGATATGCTGCAGATCATTACTAATGGCAAGTTCAAGAGTGCAGAGCACAGAGTAATAATTCCACCGACCCTCGATGCCCTTACATCAATTGCATGTTTCATTGGCACAGACGACCTCCAAAAACCATACGGGCCGTTAAAGGAGCTTATATCCGAAAACAATCCACCAATTTACAAAGAAGTCGTTTTTGGAGAGTACGTGAAACGTTACAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.21

Weight (kDa)

5.58

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 157 4.1e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 307 3.3e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 375
AciI CCGC 4 cut(s) 166, 214, 512, 687
AclI AACGTT 1 cut(s) 1021
AclWI GGATC 2 cut(s) 21, 135
AcsI RAATTY 2 cut(s) 41, 413
AcuI CTGAAG 1 cut(s) 335
AdeI CACNNNGTG 2 cut(s) 296, 787
AfaI GTAC 2 cut(s) 753, 1013
AfiI CCNNNNNNNGG 4 cut(s) 124, 241, 790, 791
AgsI TTSAA 4 cut(s) 112, 418, 545, 850
AjnI CCWGG 2 cut(s) 4, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
AluBI AGCT 9 cut(s) 22, 59, 97, 248, 434, 559, 734, 797, 961
AluI AGCT 9 cut(s) 22, 59, 97, 248, 434, 559, 734, 797, 961
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 2 cut(s) 429, 438
AlwI GGATC 2 cut(s) 21, 135
AoxI GGCC 3 cut(s) 70, 742, 947
ApeKI GCWGC 4 cut(s) 245, 340, 594, 823
ApoI RAATTY 2 cut(s) 41, 413
AseI ATTAAT 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 463, 947
AsuHPI GGTGA 3 cut(s) 100, 709, 773
AsuII TTCGAA 1 cut(s) 624
AvaII GGWCC 1 cut(s) 463
AxyI CCTNAGG 1 cut(s) 614
BanII GRGCYC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 864
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 257, 352, 606, 810
BccI CCATC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 2 cut(s) 6, 347
BcoDI GTCTC 2 cut(s) 429, 438
BfaI CTAG 2 cut(s) 467, 735
BfmI CTRYAG 4 cut(s) 505, 746, 824, 1031
BisI GCNGC 4 cut(s) 246, 341, 595, 824
BlsI GCNGC 4 cut(s) 247, 342, 596, 825
Bme1390I CCNGG 2 cut(s) 6, 347
Bme18I GGWCC 1 cut(s) 463
BmgT120I GGNCC 2 cut(s) 463, 947
BmrFI CCNGG 2 cut(s) 6, 347
BmrI ACTGGG 1 cut(s) 779
BmsI GCATC 1 cut(s) 880
BmuI ACTGGG 1 cut(s) 779
BpmI CTGGAG 2 cut(s) 44, 105
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 1015
BsaJI CCNNGG 4 cut(s) 5, 345, 354, 706
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 999, 1029
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 241, 790, 791
Bse1I ACTGG 2 cut(s) 656, 785
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 2 cut(s) 6, 347
BseDI CCNNGG 4 cut(s) 5, 345, 354, 706
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 4 cut(s) 124, 241, 790, 791
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 240, 250
BseNI ACTGG 2 cut(s) 656, 785
BseXI GCAGC 4 cut(s) 257, 352, 606, 810
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 3 cut(s) 72, 744, 949
BsiHKAI GWGCWC 1 cut(s) 864
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 4 cut(s) 124, 241, 790, 791
BsmAI GTCTC 2 cut(s) 429, 438
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 2 cut(s) 321, 697
BsnI GGCC 3 cut(s) 72, 744, 949
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 672, 864
Bsp143I GATC 4 cut(s) 13, 127, 184, 828
BspACI CCGC 4 cut(s) 166, 214, 512, 687
BspANI GGCC 3 cut(s) 72, 744, 949
BspCNI CTCAG 2 cut(s) 241, 249
BspFNI CGCG 1 cut(s) 375
BspHI TCATGA 2 cut(s) 16, 334
BspMAI CTGCAG 2 cut(s) 509, 828
BspPI GGATC 2 cut(s) 21, 135
BspT104I TTCGAA 1 cut(s) 624
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 2 cut(s) 656, 785
BssECI CCNNGG 4 cut(s) 5, 345, 354, 706
BssMI GATC 4 cut(s) 13, 127, 184, 828
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 6, 347
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 432
BstBAI YACGTR 1 cut(s) 1015
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 2 cut(s) 643, 804
BstDEI CTNAG 4 cut(s) 236, 249, 590, 614
BstEII GGTNACC 1 cut(s) 88
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 1 cut(s) 375
BstKTI GATC 4 cut(s) 16, 130, 187, 831
BstMAI GTCTC 2 cut(s) 429, 438
BstMBI GATC 4 cut(s) 13, 127, 184, 828
BstMWI GCNNNNNNNGC 1 cut(s) 803
BstNI CCWGG 2 cut(s) 6, 347
BstNSI RCATGY 2 cut(s) 645, 912
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 4, 345
BstSFI CTRYAG 4 cut(s) 505, 746, 824, 1031
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 257, 352, 606, 810
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 2 cut(s) 152, 438
BstYI RGATCY 1 cut(s) 127
Bsu36I CCTNAGG 1 cut(s) 614
BsuRI GGCC 3 cut(s) 72, 744, 949
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 2 cut(s) 643, 804
CciI TCATGA 2 cut(s) 16, 334
Cfr13I GGNCC 2 cut(s) 463, 947
Csp6I GTAC 2 cut(s) 752, 1012
CviAII CATG 4 cut(s) 17, 335, 642, 909
CviQI GTAC 2 cut(s) 752, 1012
DdeI CTNAG 4 cut(s) 236, 249, 590, 614
DpnI GATC 4 cut(s) 15, 129, 186, 830
DpnII GATC 4 cut(s) 13, 127, 184, 828
DraIII CACNNNGTG 2 cut(s) 296, 787
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
EciI GGCGGA 2 cut(s) 527, 702
Eco130I CCWWGG 1 cut(s) 354
Eco147I AGGCCT 2 cut(s) 72, 744
Eco24I GRGCYC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 335
Eco81I CCTNAGG 1 cut(s) 614
Eco91I GGTNACC 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 4, 345
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 672
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 4 cut(s) 20, 338, 645, 912
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 121
FatI CATG 4 cut(s) 16, 334, 641, 908
Fnu4HI GCNGC 4 cut(s) 246, 341, 595, 824
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 4 cut(s) 246, 341, 595, 824
FspBI CTAG 2 cut(s) 467, 735
GluI GCNGC 4 cut(s) 246, 341, 595, 824
GsuI CTGGAG 2 cut(s) 44, 105
HaeIII GGCC 3 cut(s) 72, 744, 949
Hin1II CATG 4 cut(s) 20, 338, 645, 912
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 4 cut(s) 80, 578, 626, 679
HphI GGTGA 3 cut(s) 100, 709, 773
Hpy166II GTNNAC 2 cut(s) 277, 394
Hpy188I TCNGA 6 cut(s) 239, 270, 577, 703, 817, 970
Hpy188III TCNNGA 6 cut(s) 17, 122, 188, 335, 382, 850
Hpy8I GTNNAC 2 cut(s) 277, 394
Hpy99I CGWCG 2 cut(s) 50, 330
HpyAV CCTTC 4 cut(s) 310, 382, 544, 734
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 2 cut(s) 1014, 1021
HpyCH4V TGCA 6 cut(s) 410, 459, 507, 826, 857, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 803
HpyF3I CTNAG 4 cut(s) 236, 249, 590, 614
HpySE526I ACGT 2 cut(s) 1014, 1021
Hsp92II CATG 4 cut(s) 20, 338, 645, 912
Kzo9I GATC 4 cut(s) 13, 127, 184, 828
LmnI GCTCC 4 cut(s) 102, 216, 794, 958
Lsp1109I GCAGC 4 cut(s) 257, 352, 606, 810
LweI GCATC 1 cut(s) 880
MaeI CTAG 2 cut(s) 467, 735
MaeII ACGT 2 cut(s) 1014, 1021
MaeIII GTNAC 2 cut(s) 88, 1022
MalI GATC 4 cut(s) 15, 129, 186, 830
MboI GATC 4 cut(s) 13, 127, 184, 828
MboII GAAGA 5 cut(s) 41, 203, 397, 419, 482
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 672, 864
MluCI AATT 7 cut(s) 41, 413, 566, 570, 873, 903, 984
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 2 cut(s) 569, 953
MslI CAYNNNNRTG 3 cut(s) 150, 640, 762
MspA1I CMGCKG 1 cut(s) 248
MspR9I CCNGG 2 cut(s) 6, 347
MunI CAATTG 1 cut(s) 903
Mva1269I GAATGC 2 cut(s) 321, 697
MvaI CCWGG 2 cut(s) 6, 347
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 1 cut(s) 803
NdeII GATC 4 cut(s) 13, 127, 184, 828
NlaIII CATG 4 cut(s) 20, 338, 645, 912
NmeAIII GCCGAG 2 cut(s) 663, 687
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 2 cut(s) 645, 912
NspV TTCGAA 1 cut(s) 624
PaeI GCATGC 1 cut(s) 645
PagI TCATGA 2 cut(s) 16, 334
PceI AGGCCT 2 cut(s) 72, 744
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 204
PctI GAATGC 2 cut(s) 321, 697
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 3 cut(s) 80, 578, 626
PkrI GCNGC 4 cut(s) 247, 342, 596, 825
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 1015
PpuMI RGGWCCY 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 1021
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 4, 345
PspEI GGTNACC 1 cut(s) 88
PspGI CCWGG 2 cut(s) 4, 345
PspPI GGNCC 2 cut(s) 463, 947
PspPPI RGGWCCY 1 cut(s) 463
PstI CTGCAG 2 cut(s) 509, 828
PsuI RGATCY 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 2 cut(s) 753, 1013
RsaNI GTAC 2 cut(s) 752, 1012
RseI CAYNNNNRTG 3 cut(s) 150, 640, 762
SaqAI TTAA 2 cut(s) 569, 953
SatI GCNGC 4 cut(s) 246, 341, 595, 824
Sau3AI GATC 4 cut(s) 13, 127, 184, 828
Sau96I GGNCC 2 cut(s) 463, 947
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 2 cut(s) 6, 347
SduI GDGCHC 2 cut(s) 672, 864
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 4 cut(s) 505, 746, 824, 1031
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 1 cut(s) 463
SmiMI CAYNNNNRTG 3 cut(s) 150, 640, 762
SphI GCATGC 1 cut(s) 645
Sse9I AATT 7 cut(s) 41, 413, 566, 570, 873, 903, 984
SseBI AGGCCT 2 cut(s) 72, 744
SsiI CCGC 4 cut(s) 166, 214, 512, 687
SspMI CTAG 2 cut(s) 467, 735
StuI AGGCCT 2 cut(s) 72, 744
StyD4I CCNGG 2 cut(s) 4, 345
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 2 cut(s) 1017, 1024
TaqI TCGA 8 cut(s) 45, 189, 198, 325, 486, 624, 629, 888
TasI AATT 7 cut(s) 41, 413, 566, 570, 873, 903, 984
TfiI GAWTC 3 cut(s) 80, 578, 626
Tru1I TTAA 2 cut(s) 569, 953
Tru9I TTAA 2 cut(s) 569, 953
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 4 cut(s) 245, 340, 594, 823
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 6 cut(s) 50, 323, 545, 703, 746, 904
TspRI CASTG 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 463
VspI ATTAAT 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 2 cut(s) 41, 413
XceI RCATGY 2 cut(s) 645, 912
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 2 cut(s) 467, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.