Rh6BG314400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000837
Physical Location & Seq
Forward (+)
54464021 .. 54466527
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG314400.1

Sequence Viewer

Length: 993 bp
ATGGCAGATGCAGACTACATGGTAGAAAAAGAAAAAGAACTGTATGCGTTTGATGACCTTAAAATAGGTGTCAAAGGGCTCTCAGACTCCGGCGTGGCCAAGATTCCCAGAATCTTCATCCACCCACCGGAGACTGTAAAGAACACAGCACCAGATGATGGTGTCAAACTTCAAATCCCAATTGTTGATCTCAGATGCATTGCCAATGATCACGCTCGAGAAGGAATAGTGAAGGCAATTAAAGATGCATGTGAAACATGGGGGTTCTTTCAGATAGTCAACCATGGAGTTCCACTTGGCACTTGCTGTTATGGAGGAGATGCTTCAAGCAGATTGGAGAAGGTGTCCTGCAGAGCCGTAGATGATAAATGGGACTTTGAAGCACTGCCTCAAGTGTGCAGACGAGAAATAAGTGAGTACATCAAAAACTTGATTGAACTTCAGAATGTGCTATCCGAATTACTATCGGAAGCATTAGGGCTTAGCAAGGATCACCTAGCACGCATGGGGTGCTTGAAAGCTTTCTCATTATCATGCTTCTATTATCCAATTTGCCCCGAGCCTGAACTGACTCTAGGCACAATCAAACACTCAGATCCCTCATTTCTTACCTTAGTCCTGCAAGACGAGATTGGAGGGCTCCAAGTTCTCCATAAGGATCAATGGGTTGACGTGCCGCCAGTGGATGGAGCATTTGTAGCAAATCTAGGTGACCTTATGCAGCTCGTTACTAATAACAAGTTCAAGAGTGTGGAACATAGAGTGCTAGCTACCCCGCTTGTCAAGCCACGAATATCAGTTGCATCTTTCTTTGCTCCTATGGATAGCGACAAACGTGTCAAGCCATACGGGCCTATAAAGGAGCTTCTATCTGAGAACAATCCCCCAATATACAGGGACACCCTTTACGGAGAATATAGTCAATATACTGGGTATTATAGAGTTAATGGACAAAATGGCAGTTCTGCCCTGCCTAATTTCAGACTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

330

Amino Acids

36.82

Weight (kDa)

5.55

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 99 1.1e-10 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 178 - 273 7.8e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 836
AccB7I CCANNNNNTGG 2 cut(s) 158, 686
AciI CCGC 2 cut(s) 677, 776
AclWI GGATC 3 cut(s) 498, 590, 666
AcoI YGGCCR 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 425
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 127, 158, 686
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 6 cut(s) 173, 327, 380, 437, 517, 745
AjiI CACGTC 1 cut(s) 673
AluBI AGCT 4 cut(s) 521, 724, 770, 865
AluI AGCT 4 cut(s) 521, 724, 770, 865
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 3 cut(s) 498, 590, 666
Ama87I CYCGRG 2 cut(s) 216, 557
AoxI GGCC 2 cut(s) 96, 851
ApeKI GCWGC 1 cut(s) 721
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 485, 722
AsuNHI GCTAGC 1 cut(s) 766
AvaI CYCGRG 2 cut(s) 216, 557
BalI TGGCCA 1 cut(s) 98
BanII GRGCYC 2 cut(s) 81, 642
BbvI GCAGC 1 cut(s) 733
BccI CCATC 2 cut(s) 152, 680
BceAI ACGGC 1 cut(s) 341
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 4 cut(s) 497, 575, 707, 767
BfmI CTRYAG 1 cut(s) 349
BglI GCCNNNNNGGC 1 cut(s) 850
BisI GCNGC 2 cut(s) 677, 722
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 2 cut(s) 678, 723
BmeT110I CYCGRG 2 cut(s) 216, 557
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 851
BmiI GGNNCC 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 4 cut(s) 185, 235, 310, 812
BmtI GCTAGC 1 cut(s) 770
BmuI ACTGGG 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 283
BsaWI WCCGGW 1 cut(s) 127
BsaXI ACNNNNNCTCC 4 cut(s) 329, 359, 681, 711
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 158, 686
Bse1I ACTGG 2 cut(s) 680, 934
Bse3DI GCAATG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 2 cut(s) 117, 691
BseLI CCNNNNNNNGG 3 cut(s) 127, 158, 686
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 4 cut(s) 96, 205, 606, 864
BseNI ACTGG 2 cut(s) 680, 934
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 733
BsgI GTGCAG 1 cut(s) 418
BshFI GGCC 2 cut(s) 98, 853
BsiHKCI CYCGRG 2 cut(s) 216, 557
BsiSI CCGG 2 cut(s) 90, 128
BslFI GGGAC 2 cut(s) 386, 911
BslI CCNNNNNNNGG 3 cut(s) 127, 158, 686
BsmAI GTCTC 1 cut(s) 125
BsmFI GGGAC 2 cut(s) 386, 911
BsnI GGCC 2 cut(s) 98, 853
BsoBI CYCGRG 2 cut(s) 216, 557
Bsp1286I GDGCHC 2 cut(s) 81, 642
Bsp143I GATC 5 cut(s) 187, 208, 490, 595, 658
Bsp1720I GCTNAGC 1 cut(s) 482
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 2 cut(s) 677, 776
BspANI GGCC 2 cut(s) 98, 853
BspCNI CTCAG 4 cut(s) 95, 204, 605, 865
BspLI GGNNCC 1 cut(s) 641
BspMAI CTGCAG 1 cut(s) 353
BspOI GCTAGC 1 cut(s) 770
BspPI GGATC 3 cut(s) 498, 590, 666
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 2 cut(s) 680, 934
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 5 cut(s) 187, 208, 490, 595, 658
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 42, 136
BstAPI GCANNNNNTGC 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 502, 768
BstDEI CTNAG 6 cut(s) 82, 191, 482, 592, 613, 873
BstDSI CCRYGG 1 cut(s) 283
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 2 cut(s) 117, 691
BstKTI GATC 5 cut(s) 190, 211, 493, 598, 661
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 5 cut(s) 187, 208, 490, 595, 658
BstMWI GCNNNNNNNGC 4 cut(s) 510, 698, 784, 850
BstNSI RCATGY 1 cut(s) 252
BstPI GGTNACC 1 cut(s) 710
BstSFI CTRYAG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 2 cut(s) 98, 853
BtgI CCRYGG 1 cut(s) 283
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 2 cut(s) 117, 691
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 2 cut(s) 383, 687
Cac8I GCNNGC 2 cut(s) 502, 768
Cfr13I GGNCC 1 cut(s) 851
Csp6I GTAC 1 cut(s) 418
CviAII CATG 6 cut(s) 19, 249, 258, 284, 505, 534
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 6 cut(s) 82, 191, 482, 592, 613, 873
DpnI GATC 5 cut(s) 189, 210, 492, 597, 660
DpnII GATC 5 cut(s) 187, 208, 490, 595, 658
DrdI GACNNNNNNGTC 1 cut(s) 836
DseDI GACNNNNNNGTC 1 cut(s) 836
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 2 cut(s) 81, 642
Eco57I CTGAAG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 216, 557
Eco91I GGTNACC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 710
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 2 cut(s) 200, 250
EcoT38I GRGCYC 2 cut(s) 81, 642
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 6 cut(s) 22, 252, 261, 287, 508, 537
FaqI GGGAC 2 cut(s) 386, 911
FatI CATG 6 cut(s) 18, 248, 257, 283, 504, 533
FauI CCCGC 1 cut(s) 783
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 677, 722
FokI GGATG 2 cut(s) 104, 698
FriOI GRGCYC 2 cut(s) 81, 642
Fsp4HI GCNGC 2 cut(s) 677, 722
FspBI CTAG 4 cut(s) 497, 575, 707, 767
GluI GCNGC 2 cut(s) 677, 722
HaeIII GGCC 2 cut(s) 98, 853
HapII CCGG 2 cut(s) 90, 128
Hin1II CATG 6 cut(s) 22, 252, 261, 287, 508, 537
HincII GTYRAC 2 cut(s) 280, 670
HindII GTYRAC 2 cut(s) 280, 670
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 4 cut(s) 86, 103, 111, 571
HpaII CCGG 2 cut(s) 90, 128
HphI GGTGA 2 cut(s) 485, 722
Hpy166II GTNNAC 2 cut(s) 280, 670
Hpy188I TCNGA 9 cut(s) 85, 194, 273, 444, 457, 469, 595, 874, 983
Hpy188III TCNNGA 2 cut(s) 218, 745
Hpy8I GTNNAC 2 cut(s) 280, 670
HpyAV CCTTC 3 cut(s) 215, 226, 334
HpyCH4III ACNGT 2 cut(s) 42, 136
HpyCH4IV ACGT 2 cut(s) 672, 835
HpyCH4V TGCA 9 cut(s) 11, 198, 248, 351, 399, 622, 721, 803, 988
HpyF10VI GCNNNNNNNGC 4 cut(s) 510, 698, 784, 850
HpyF3I CTNAG 6 cut(s) 82, 191, 482, 592, 613, 873
HpySE526I ACGT 2 cut(s) 672, 835
Hsp92II CATG 6 cut(s) 22, 252, 261, 287, 508, 537
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 5 cut(s) 187, 208, 490, 595, 658
LmnI GCTCC 4 cut(s) 645, 689, 820, 862
Lsp1109I GCAGC 1 cut(s) 733
LweI GCATC 4 cut(s) 185, 235, 310, 812
MaeI CTAG 4 cut(s) 497, 575, 707, 767
MaeII ACGT 2 cut(s) 672, 835
MaeIII GTNAC 2 cut(s) 710, 727
MalI GATC 5 cut(s) 189, 210, 492, 597, 660
MboI GATC 5 cut(s) 187, 208, 490, 595, 658
MboII GAAGA 1 cut(s) 106
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 2 cut(s) 81, 642
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 5 cut(s) 180, 237, 458, 549, 976
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 80, 565
MnlI CCTC 4 cut(s) 308, 399, 610, 629
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 200, 250
MroXI GAANNNNTTC 1 cut(s) 521
MscI TGGCCA 1 cut(s) 98
MseI TTAA 3 cut(s) 60, 240, 945
MslI CAYNNNNRTG 1 cut(s) 532
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 2 cut(s) 90, 128
MunI CAATTG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 510, 698, 784, 850
NcoI CCATGG 1 cut(s) 283
NdeII GATC 5 cut(s) 187, 208, 490, 595, 658
NheI GCTAGC 1 cut(s) 766
NlaIII CATG 6 cut(s) 22, 252, 261, 287, 508, 537
NlaIV GGNNCC 1 cut(s) 641
NmuCI GTSAC 1 cut(s) 710
NsiI ATGCAT 2 cut(s) 200, 250
NspI RCATGY 1 cut(s) 252
PaeR7I CTCGAG 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 2 cut(s) 103, 111
PflMI CCANNNNNTGG 2 cut(s) 158, 686
PkrI GCNGC 2 cut(s) 678, 723
PleI GAGTC 2 cut(s) 80, 565
PpsI GAGTC 2 cut(s) 80, 565
PspEI GGTNACC 1 cut(s) 710
PspN4I GGNNCC 1 cut(s) 641
PspPI GGNCC 1 cut(s) 851
PstI CTGCAG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 532
SaqAI TTAA 3 cut(s) 60, 240, 945
SatI GCNGC 2 cut(s) 677, 722
Sau3AI GATC 5 cut(s) 187, 208, 490, 595, 658
Sau96I GGNCC 1 cut(s) 851
SchI GAGTC 2 cut(s) 80, 565
SduI GDGCHC 2 cut(s) 81, 642
SfaNI GCATC 4 cut(s) 185, 235, 310, 812
SfcI CTRYAG 1 cut(s) 349
Sfr274I CTCGAG 1 cut(s) 216
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 532
SmlI CTYRAG 2 cut(s) 216, 390
SmoI CTYRAG 2 cut(s) 216, 390
Sse9I AATT 5 cut(s) 180, 237, 458, 549, 976
SsiI CCGC 2 cut(s) 677, 776
SspMI CTAG 4 cut(s) 497, 575, 707, 767
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 42, 136
TaiI ACGT 2 cut(s) 675, 838
TaqI TCGA 1 cut(s) 217
TasI AATT 5 cut(s) 180, 237, 458, 549, 976
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 2 cut(s) 103, 111
Tru1I TTAA 3 cut(s) 60, 240, 945
Tru9I TTAA 3 cut(s) 60, 240, 945
TscAI CASTG 2 cut(s) 390, 687
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 1 cut(s) 721
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 390, 687
Van91I CCANNNNNTGG 2 cut(s) 158, 686
XceI RCATGY 1 cut(s) 252
XhoI CTCGAG 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 4 cut(s) 497, 575, 707, 767
Zsp2I ATGCAT 2 cut(s) 200, 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.