RLG00000012382

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000837
Physical Location & Seq
Reverse (-)
18127111 .. 18128535
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012382

Sequence Viewer

Length: 867 bp
ATGGCAGATGCAGACCGCATTGCAGAAAAAGAAAAAGAATTAGAATTGTGTGCGTTTGATGACCTCAAAATAGGTGTCAAAGGTCTTGCAGACTCCGGGGTGACCAGGATTCCCAGAATCTTCATCCACCCACCAGAGACTGTAAAGTACACAACACCAGAAAATGGTGTCAAACTTCAGATCCCAGTCATTGATCTCAAAGGCATTGGGAATGATCACAGTCTAGAGGAAATGGTCAATGCACTTAAAGATGCATGTGAAACATGGCGGTTCTTTCAAATAGTTAGCCATGGAGTTCCACTTGCTGCTATGGAGGAGATGCTTGATATTATTCGTAGATTCCATGAGCAGGCCCCAGAAGACAAGATGGAGCTCTACTCTCGTGATTTTAAGAGGCAGAAGCAGATTGGAGAGACACTATTCTGCAGAGCCGTCGATGAAAATTGGAACTTTGGGGCACTGCCACAAGTTTGCAGGAACAAACAAACATTCAGACCCTCATTTCTTACCTTAGTTCTACAAGACGAGATTGGGGGCCTCCAAGTCTTCCATAAAGATCAATGGGTTGATGTGCCACCAGTGAATGGAGCTTTTGTAGCAAATCTTGGTGACTTATGCAGCAAGTTCAAGAGTGGGCAACATAGAGTGCTCGCTACCTCACTTGTGAAGCCACAGATATCAGCTGCGTCTTTCTTTGTTCCCGTGGATAGTGACAAACGTGTCAAGCCATATGGACCTATAAAGGAGCTTCTATCTGAGTTCAATCCGCCAATATACAGGGACACCCTCTACAGAGAATATACCGGGTATTACAGAGTTAATGTTCAACACAGTAGCTCTGCACTGCCTAACTTCAGACTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

289

Amino Acids

32.82

Weight (kDa)

6.26

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 61 - 129 2.2e-15 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 163 - 233 5.4e-14 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 719
AccB7I CCANNNNNTGG 2 cut(s) 164, 584
AciI CCGC 3 cut(s) 16, 268, 767
AclWI GGATC 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 161, 838
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 3 cut(s) 164, 349, 584
AflIII ACRYGT 1 cut(s) 718
AgsI TTSAA 4 cut(s) 278, 628, 763, 827
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 5 cut(s) 373, 590, 683, 748, 837
AluI AGCT 5 cut(s) 373, 590, 683, 748, 837
Alw21I GWGCWC 2 cut(s) 375, 651
Alw26I GTCTC 2 cut(s) 131, 407
AlwI GGATC 1 cut(s) 175
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 2 cut(s) 351, 535
ApeKI GCWGC 3 cut(s) 305, 618, 683
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AspS9I GGNCC 3 cut(s) 352, 535, 734
AsuC2I CCSGG 2 cut(s) 97, 805
AsuHPI GGTGA 2 cut(s) 112, 620
AvaII GGWCC 1 cut(s) 734
BaeGI GKGCMC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 375
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 2 cut(s) 366, 538
Bbv12I GWGCWC 2 cut(s) 375, 651
BbvI GCAGC 3 cut(s) 292, 630, 670
BccI CCATC 1 cut(s) 361
BceAI ACGGC 1 cut(s) 416
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 1 cut(s) 106
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 97, 805
BcoDI GTCTC 2 cut(s) 131, 407
BfaI CTAG 1 cut(s) 224
BfmI CTRYAG 2 cut(s) 424, 790
BisI GCNGC 3 cut(s) 306, 619, 684
BlsI GCNGC 3 cut(s) 307, 620, 685
Bme1390I CCNGG 3 cut(s) 97, 106, 805
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 3 cut(s) 352, 535, 734
BmiI GGNNCC 2 cut(s) 354, 536
BmrFI CCNGG 3 cut(s) 97, 106, 805
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 241, 309
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 366, 538
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpuMI CCSGG 2 cut(s) 97, 805
BsaJI CCNNGG 3 cut(s) 96, 289, 702
BsaXI ACNNNNNCTCC 2 cut(s) 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 349, 584
Bse1I ACTGG 2 cut(s) 185, 578
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 3 cut(s) 96, 289, 702
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 164, 349, 584
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 747
BseNI ACTGG 2 cut(s) 185, 578
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 292, 630, 670
BsgI GTGCAG 2 cut(s) 825, 845
BshFI GGCC 2 cut(s) 353, 537
BsiHKAI GWGCWC 2 cut(s) 375, 651
BsiSI CCGG 2 cut(s) 96, 804
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 3 cut(s) 164, 349, 584
BsmAI GTCTC 2 cut(s) 131, 407
BsmFI GGGAC 1 cut(s) 794
BsnI GGCC 2 cut(s) 353, 537
Bsp1286I GDGCHC 3 cut(s) 375, 460, 651
Bsp143I GATC 4 cut(s) 180, 193, 214, 556
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 3 cut(s) 16, 268, 767
BspANI GGCC 2 cut(s) 353, 537
BspCNI CTCAG 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 354, 536
BspMAI CTGCAG 1 cut(s) 428
BspPI GGATC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 185, 578
BssECI CCNNGG 3 cut(s) 96, 289, 702
BssMI GATC 4 cut(s) 180, 193, 214, 556
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 3 cut(s) 142, 221, 833
BstC8I GCNNGC 2 cut(s) 351, 651
BstDEI CTNAG 2 cut(s) 511, 756
BstDSI CCRYGG 2 cut(s) 289, 702
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 1 cut(s) 123
BstKTI GATC 4 cut(s) 183, 196, 217, 559
BstMAI GTCTC 2 cut(s) 131, 407
BstMBI GATC 4 cut(s) 180, 193, 214, 556
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 258
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 95, 104, 803
BstSFI CTRYAG 2 cut(s) 424, 790
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 292, 630, 670
BstV2I GAAGAC 2 cut(s) 366, 538
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 2 cut(s) 353, 537
BtgI CCRYGG 2 cut(s) 289, 702
BtsCI GGATG 1 cut(s) 123
BtsI GCAGTG 2 cut(s) 458, 842
BtsIMutI CAGTG 4 cut(s) 458, 585, 842, 862
Cac8I GCNNGC 2 cut(s) 351, 651
CaiI CAGNNNCTG 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 352, 535, 734
CseI GACGC 1 cut(s) 675
Csp6I GTAC 1 cut(s) 148
CviAII CATG 4 cut(s) 255, 264, 290, 344
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 2 cut(s) 511, 756
DpnI GATC 4 cut(s) 182, 195, 216, 558
DpnII GATC 4 cut(s) 180, 193, 214, 556
DrdI GACNNNNNNGTC 1 cut(s) 719
DseDI GACNNNNNNGTC 1 cut(s) 719
EciI GGCGGA 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 375
Eco32I GATATC 1 cut(s) 678
Eco47I GGWCC 1 cut(s) 734
Eco53kI GAGCTC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 161, 838
Eco91I GGTNACC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 2 cut(s) 352, 535
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 104
EcoRV GATATC 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 4 cut(s) 258, 267, 293, 347
FaqI GGGAC 1 cut(s) 794
FatI CATG 4 cut(s) 254, 263, 289, 343
FauNDI CATATG 1 cut(s) 730
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 306, 619, 684
FokI GGATG 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 3 cut(s) 306, 619, 684
FspBI CTAG 1 cut(s) 224
GluI GCNGC 3 cut(s) 306, 619, 684
HaeIII GGCC 2 cut(s) 353, 537
HapII CCGG 2 cut(s) 96, 804
HgaI GACGC 1 cut(s) 675
Hin1II CATG 4 cut(s) 258, 267, 293, 347
HinfI GANTC 4 cut(s) 92, 109, 117, 339
HpaII CCGG 2 cut(s) 96, 804
HphI GGTGA 2 cut(s) 112, 620
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 4 cut(s) 180, 494, 757, 857
Hpy188III TCNNGA 3 cut(s) 224, 383, 628
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 1 cut(s) 437
HpyCH4III ACNGT 3 cut(s) 142, 221, 833
HpyCH4IV ACGT 1 cut(s) 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 2 cut(s) 511, 756
HpySE526I ACGT 1 cut(s) 718
Hsp92II CATG 4 cut(s) 258, 267, 293, 347
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 180, 193, 214, 556
LmnI GCTCC 3 cut(s) 370, 587, 745
Lsp1109I GCAGC 3 cut(s) 292, 630, 670
LweI GCATC 2 cut(s) 241, 309
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 718
MaeIII GTNAC 3 cut(s) 100, 608, 710
MalI GATC 4 cut(s) 182, 195, 216, 558
MboI GATC 4 cut(s) 180, 193, 214, 556
MboII GAAGA 3 cut(s) 112, 371, 538
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 3 cut(s) 375, 460, 651
MluCI AATT 3 cut(s) 38, 44, 442
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 8 cut(s) 74, 220, 307, 387, 508, 548, 667, 797
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 3 cut(s) 246, 390, 819
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 2 cut(s) 96, 804
MspR9I CCNGG 3 cut(s) 97, 106, 805
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 596
NciI CCSGG 2 cut(s) 97, 805
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 730
NdeII GATC 4 cut(s) 180, 193, 214, 556
NlaIII CATG 4 cut(s) 258, 267, 293, 347
NlaIV GGNNCC 2 cut(s) 354, 536
NmuCI GTSAC 3 cut(s) 100, 608, 710
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 1 cut(s) 258
PfeI GAWTC 3 cut(s) 109, 117, 339
PflMI CCANNNNNTGG 2 cut(s) 164, 584
PkrI GCNGC 3 cut(s) 307, 620, 685
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 104
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 354, 536
PspPI GGNCC 3 cut(s) 352, 535, 734
PstI CTGCAG 1 cut(s) 428
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 246, 390, 819
SatI GCNGC 3 cut(s) 306, 619, 684
Sau3AI GATC 4 cut(s) 180, 193, 214, 556
Sau96I GGNCC 3 cut(s) 352, 535, 734
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 97, 106, 805
SduI GDGCHC 3 cut(s) 375, 460, 651
SfaNI GCATC 2 cut(s) 241, 309
SfcI CTRYAG 2 cut(s) 424, 790
SinI GGWCC 1 cut(s) 734
Sse9I AATT 3 cut(s) 38, 44, 442
SsiI CCGC 3 cut(s) 16, 268, 767
SspMI CTAG 1 cut(s) 224
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 3 cut(s) 95, 104, 803
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 142, 221, 833
TaiI ACGT 1 cut(s) 721
TaqI TCGA 1 cut(s) 435
TasI AATT 3 cut(s) 38, 44, 442
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 3 cut(s) 109, 117, 339
Tru1I TTAA 3 cut(s) 246, 390, 819
Tru9I TTAA 3 cut(s) 246, 390, 819
TscAI CASTG 3 cut(s) 465, 585, 849
TseFI GTSAC 3 cut(s) 100, 608, 710
TseI GCWGC 3 cut(s) 305, 618, 683
Tsp45I GTSAC 3 cut(s) 100, 608, 710
TspDTI ATGAA 2 cut(s) 112, 453
TspRI CASTG 3 cut(s) 465, 585, 849
Van91I CCANNNNNTGG 2 cut(s) 164, 584
VpaK11BI GGWCC 1 cut(s) 734
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 258
XspI CTAG 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.