Rh6BG314500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000837
Physical Location & Seq
Forward (+)
54466564 .. 54468077
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG314500.1

Sequence Viewer

Length: 459 bp
ATGGATGAGATCTGGCTTTCAGACTCTGAGGTATCCAAGATTCCCAGAATTTTCATCCACCCACCAGAGACTGTAAAGAACAAAGCACCAGAAAATGGTGTCAAACTTCAAATCCCAGTTATTGATCTCAAAGACGTCGGGAATGAACACAGTCGAGAGGAAACGGTCAATGCAATTAAAGATGCATGTGAAACATGGAGGTTCTTTCAGATAGTTTACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGTATTCGTAGATTTCATGAGCAGCCGGCAGAAGACAAGATGGAGTCGTACTCTCGTGACTTCAAGAATCCGGTCAACTTTTATTGCAGTGGAGATCTGAAAGTGCGTGCAAAATCAGCAGCAGACTGGAGAGACACCTTATTCTGCAGAGAAGTAGATGATGAAAGGGATTTTGAAGCACTGCCTCAAGTCTGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

152

Amino Acids

17.73

Weight (kDa)

5.05

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 140 8.9e-21 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 95
AcsI RAATTY 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 4 cut(s) 110, 257, 322, 434
Alw26I GTCTC 2 cut(s) 62, 384
AlwNI CAGNNNCTG 2 cut(s) 26, 71
ApeKI GCWGC 2 cut(s) 280, 377
ApoI RAATTY 1 cut(s) 48
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 292, 389
BccI CCATC 1 cut(s) 292
BciVI GTATCC 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 62, 384
BfmI CTRYAG 2 cut(s) 403, 451
BfuAI ACCTGC 1 cut(s) 444
BfuI GTATCC 1 cut(s) 43
BglII AGATCT 2 cut(s) 9, 352
BisI GCNGC 2 cut(s) 281, 378
BlsI GCNGC 2 cut(s) 282, 379
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 2 cut(s) 172, 240
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 2 cut(s) 116, 389
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 10, 54
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 116, 389
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 292, 389
BsiSI CCGG 2 cut(s) 284, 329
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 62, 384
Bsp143I GATC 3 cut(s) 9, 124, 352
Bsp19I CCATGG 1 cut(s) 220
BspCNI CTCAG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 274
BspMAI CTGCAG 2 cut(s) 407, 455
BspMI ACCTGC 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 2 cut(s) 116, 389
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 9, 124, 352
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 312
Bst4CI ACNGT 3 cut(s) 73, 152, 166
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 285, 366
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 2 cut(s) 10, 54
BstKTI GATC 3 cut(s) 12, 127, 355
BstMAI GTCTC 2 cut(s) 62, 384
BstMBI GATC 3 cut(s) 9, 124, 352
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 189
BstSFI CTRYAG 2 cut(s) 403, 451
BstV1I GCAGC 2 cut(s) 292, 389
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 9, 352
BstYI RGATCY 2 cut(s) 9, 352
BsuI GTATCC 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 10, 54
BtsI GCAGTG 2 cut(s) 352, 437
BtsIMutI CAGTG 2 cut(s) 352, 437
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 285, 366
CaiI CAGNNNCTG 2 cut(s) 26, 71
CciI TCATGA 1 cut(s) 274
Cfr10I RCCGGY 1 cut(s) 283
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 186, 195, 221, 275
CviJI RGCY 2 cut(s) 16, 283
CviKI_1 RGCY 2 cut(s) 16, 283
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 27
DpnI GATC 3 cut(s) 11, 126, 354
DpnII GATC 3 cut(s) 9, 124, 352
Eco130I CCWWGG 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 4 cut(s) 189, 198, 224, 278
FaiI YATR 5 cut(s) 187, 196, 222, 242, 276
FatI CATG 4 cut(s) 185, 194, 220, 274
Fnu4HI GCNGC 2 cut(s) 281, 378
FokI GGATG 2 cut(s) 17, 41
Fsp4HI GCNGC 2 cut(s) 281, 378
GluI GCNGC 2 cut(s) 281, 378
GsuI CTGGAG 1 cut(s) 406
HapII CCGG 2 cut(s) 284, 329
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 4 cut(s) 189, 198, 224, 278
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 4 cut(s) 23, 40, 302, 325
HpaII CCGG 2 cut(s) 284, 329
Hpy166II GTNNAC 2 cut(s) 217, 334
Hpy188I TCNGA 4 cut(s) 22, 28, 210, 357
Hpy188III TCNNGA 5 cut(s) 139, 155, 275, 314, 322
Hpy8I GTNNAC 2 cut(s) 217, 334
Hpy99I CGWCG 1 cut(s) 140
HpyCH4III ACNGT 3 cut(s) 73, 152, 166
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 6 cut(s) 173, 185, 345, 368, 405, 453
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 4 cut(s) 189, 198, 224, 278
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Kzo9I GATC 3 cut(s) 9, 124, 352
LpnPI CCDG 8 cut(s) 58, 78, 102, 129, 297, 342, 370, 439
Lsp1109I GCAGC 2 cut(s) 292, 389
LweI GCATC 2 cut(s) 172, 240
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 314
MalI GATC 3 cut(s) 11, 126, 354
MboI GATC 3 cut(s) 9, 124, 352
MboII GAAGA 1 cut(s) 302
MflI RGATCY 2 cut(s) 9, 352
MluCI AATT 2 cut(s) 48, 174
MlyI GAGTC 2 cut(s) 17, 311
MnlI CCTC 5 cut(s) 22, 151, 192, 238, 453
Mph1103I ATGCAT 1 cut(s) 187
MroNI GCCGGC 1 cut(s) 283
MseI TTAA 1 cut(s) 177
MspI CCGG 2 cut(s) 284, 329
MwoI GCNNNNNNNGC 1 cut(s) 374
NaeI GCCGGC 1 cut(s) 285
NcoI CCATGG 1 cut(s) 220
NdeII GATC 3 cut(s) 9, 124, 352
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 4 cut(s) 189, 198, 224, 278
NmuCI GTSAC 1 cut(s) 314
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 1 cut(s) 189
PagI TCATGA 1 cut(s) 274
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 40, 325
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 2 cut(s) 282, 379
PleI GAGTC 2 cut(s) 17, 310
PpsI GAGTC 2 cut(s) 17, 310
PstI CTGCAG 2 cut(s) 407, 455
PstNI CAGNNNCTG 2 cut(s) 26, 71
PsuI RGATCY 2 cut(s) 9, 352
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 2 cut(s) 281, 378
Sau3AI GATC 3 cut(s) 9, 124, 352
SchI GAGTC 2 cut(s) 17, 311
SetI ASST 6 cut(s) 33, 138, 203, 262, 398, 458
SfaNI GCATC 2 cut(s) 172, 240
SfcI CTRYAG 2 cut(s) 403, 451
SmlI CTYRAG 1 cut(s) 444
SmoI CTYRAG 1 cut(s) 444
Sse9I AATT 2 cut(s) 48, 174
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 3 cut(s) 73, 152, 166
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 154
TasI AATT 2 cut(s) 48, 174
TfiI GAWTC 2 cut(s) 40, 325
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 2 cut(s) 352, 444
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 280, 377
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 4 cut(s) 43, 159, 263, 435
TspRI CASTG 2 cut(s) 352, 444
Van91I CCANNNNNTGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 48
XceI RCATGY 1 cut(s) 189
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.