pycom10g16170

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000837
Physical Location & Seq
Reverse (-)
19343907 .. 19345213
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g16170.2

Sequence Viewer

Length: 1041 bp
ATGGTGGATGTGTTGGATCATGAGCTGGAGAAGGAACTGAAACAGTTCGACGAAACCAAAGCTGGTGTGAAAGGGCTTGTAGATTCTGGGGTGACCAAGCTCCCAAGAATGTTCAAACACCCTCCGGAGTGTCTACCATCCCCGAACCAAAACAATGGAATTCACGACCTGCAAGTTCCTGTCATCGATCTCAAAGATGCGGAGAACAACGAAAGGAGAAGCGAAATCATCAGTAACCTCCGCAAAGCAGCTGAAGAATGGGGTTTCTTTCAAATTGTGAATCATCCCATTCCACTTGACGTCATGGATGATGTGCTGAAAGGTGTTCGGCGGTTTCATGAGCAGCCCCAGGAAGCCAAGGAGGAGTGGTATTCGCGTGATTTCACGAAGAAGGTCAACTTCTTCTGCAATGGAGAATTGAAGGTAGACACTCCAGCTGACTGGAGAGACACCTTGTCATGCAAGGTCCTCGAAGATGAACGCAACTTTGAAGAAATCCCTGAAGTCTGCAGAACGGAAATACGTGAGTATATGAAGTACATCGTTCAAGTGAAGGAGAAGCTATCCGGATTATTTTCGGAAGCTTTAGGCCTAAGCAGTGATTACCTTGAAAATCTAAGGTGCTTCAAATCTAGGTCATTGGCATGCCACTACTACCCGGTATGCCCGGAGCCCCATTTAACCCTAGGAGGAACCAAACACTCCGATCTCGGCTTTCTAACCTTGCTCTTGCAAGATAGTGCTGGCCTACAAGTTCTTCATCAAAATATTTGGATTGATGTTCCCCCAGTGGAGGGAGCTTTGCTCATCAATCTAGCCGACATGTTGCAGTTTATTACTAATGGCAAGTTCAAGAGTGTACAGCACAGAGTACTGATGCCACTGTCGACCCTCGAGCCCCGTACGTCAATTGCATGTTTTGTTGGCACCGACGACCTCCAAAAACCTTACGGTCCGTTAAAGGAGCTCATCTCCGAAAACAATCCGGCAAAATACGAAGATGTCTGTTTTGGAGAATACATGAAACGTTATAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

347

Amino Acids

39.76

Weight (kDa)

5.33

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 926
AccI GTMKAC 3 cut(s) 133, 426, 887
AccII CGCG 1 cut(s) 376
AccIII TCCGGA 2 cut(s) 124, 566
AciI CCGC 3 cut(s) 200, 241, 331
AclI AACGTT 1 cut(s) 1027
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 1 cut(s) 159
AcuI CTGAAG 2 cut(s) 273, 522
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 4 cut(s) 539, 861, 873, 904
AfiI CCNNNNNNNGG 2 cut(s) 793, 794
AflIII ACRYGT 1 cut(s) 822
AgsI TTSAA 8 cut(s) 115, 272, 421, 491, 548, 611, 628, 853
AhdI GACNNNNNGTC 1 cut(s) 454
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 9 cut(s) 25, 62, 100, 251, 437, 562, 584, 800, 967
AluI AGCT 9 cut(s) 25, 62, 100, 251, 437, 562, 584, 800, 967
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 1 cut(s) 441
AlwI GGATC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 893
Aor13HI TCCGGA 2 cut(s) 124, 566
AoxI GGCC 2 cut(s) 589, 745
ApeKI GCWGC 2 cut(s) 248, 343
ApoI RAATTY 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 44
AspA2I CCTAGG 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 466, 953
AsuC2I CCSGG 2 cut(s) 659, 668
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 893
AvaII GGWCC 2 cut(s) 466, 953
AvrII CCTAGG 1 cut(s) 685
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 3 cut(s) 675, 900, 969
Bbv12I GWGCWC 1 cut(s) 969
BbvI GCAGC 2 cut(s) 260, 355
BccI CCATC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 2 cut(s) 659, 668
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 3 cut(s) 633, 686, 815
BfmI CTRYAG 1 cut(s) 508
BfuAI ACCTGC 1 cut(s) 177
BisI GCNGC 2 cut(s) 249, 344
BlnI CCTAGG 1 cut(s) 685
BlsI GCNGC 2 cut(s) 250, 345
BmcAI AGTACT 1 cut(s) 873
Bme1390I CCNGG 3 cut(s) 350, 659, 668
Bme18I GGWCC 2 cut(s) 466, 953
BmeRI GACNNNNNGTC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 893
BmgT120I GGNCC 2 cut(s) 466, 953
BmiI GGNNCC 3 cut(s) 672, 694, 928
BmrFI CCNGG 3 cut(s) 350, 659, 668
BmrI ACTGGG 1 cut(s) 782
BmsI GCATC 2 cut(s) 187, 867
BmuI ACTGGG 1 cut(s) 782
BplI GAGNNNNNCTC 4 cut(s) 789, 821, 956, 988
BpmI CTGGAG 3 cut(s) 47, 417, 463
Bpu10I CCTNAGC 1 cut(s) 593
BpuMI CCSGG 2 cut(s) 659, 668
Bsa29I ATCGAT 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 3 cut(s) 348, 357, 685
BsaWI WCCGGW 2 cut(s) 124, 566
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 793, 794
Bse1I ACTGG 2 cut(s) 446, 788
Bse3DI GCAATG 1 cut(s) 415
BseAI TCCGGA 2 cut(s) 124, 566
BseBI CCWGG 1 cut(s) 350
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 348, 357, 685
BseGI GGATG 4 cut(s) 13, 137, 283, 313
BseLI CCNNNNNNNGG 2 cut(s) 793, 794
BseMI GCAATG 1 cut(s) 415
BseNI ACTGG 2 cut(s) 446, 788
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 260, 355
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 2 cut(s) 591, 747
BshNI GGYRCC 1 cut(s) 926
BshVI ATCGAT 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 969
BsiHKCI CYCGRG 1 cut(s) 893
BsiSI CCGG 5 cut(s) 125, 567, 659, 668, 986
BsiWI CGTACG 1 cut(s) 902
BslI CCNNNNNNNGG 2 cut(s) 793, 794
BsmAI GTCTC 1 cut(s) 441
BsnI GGCC 2 cut(s) 591, 747
BsoBI CYCGRG 1 cut(s) 893
Bsp1286I GDGCHC 3 cut(s) 675, 900, 969
Bsp13I TCCGGA 2 cut(s) 124, 566
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 3 cut(s) 16, 187, 706
BspACI CCGC 3 cut(s) 200, 241, 331
BspANI GGCC 2 cut(s) 591, 747
BspDI ATCGAT 1 cut(s) 186
BspEI TCCGGA 2 cut(s) 124, 566
BspFNI CGCG 1 cut(s) 376
BspHI TCATGA 2 cut(s) 19, 337
BspLI GGNNCC 3 cut(s) 672, 694, 928
BspMAI CTGCAG 1 cut(s) 512
BspMI ACCTGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 926
BsrDI GCAATG 1 cut(s) 415
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 2 cut(s) 446, 788
BssECI CCNNGG 3 cut(s) 348, 357, 685
BssMI GATC 3 cut(s) 16, 187, 706
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 357, 685
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 3 cut(s) 45, 885, 953
BstACI GRCGYC 1 cut(s) 300
BstAUI TGTACA 1 cut(s) 859
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 2 cut(s) 646, 745
BstDEI CTNAG 2 cut(s) 593, 617
BstEII GGTNACC 1 cut(s) 91
BstF5I GGATG 4 cut(s) 13, 137, 283, 313
BstFNI CGCG 1 cut(s) 376
BstKTI GATC 3 cut(s) 19, 190, 709
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 3 cut(s) 16, 187, 706
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 3 cut(s) 648, 826, 918
BstPI GGTNACC 1 cut(s) 91
BstSCI CCNGG 3 cut(s) 348, 657, 666
BstSFI CTRYAG 1 cut(s) 508
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 2 cut(s) 260, 355
BstXI CCANNNNNNTGG 2 cut(s) 155, 441
Bsu15I ATCGAT 1 cut(s) 186
BsuRI GGCC 2 cut(s) 591, 747
BsuTUI ATCGAT 1 cut(s) 186
BtsCI GGATG 4 cut(s) 13, 137, 283, 313
BtsI GCAGTG 1 cut(s) 604
BtsIMutI CAGTG 3 cut(s) 604, 795, 881
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 646, 745
CciI TCATGA 2 cut(s) 19, 337
Cfr13I GGNCC 2 cut(s) 466, 953
ClaI ATCGAT 1 cut(s) 186
CpoI CGGWCCG 1 cut(s) 953
Csp6I GTAC 4 cut(s) 538, 860, 872, 903
CspI CGGWCCG 1 cut(s) 953
CviAII CATG 8 cut(s) 20, 304, 338, 459, 645, 823, 915, 1021
CviQI GTAC 4 cut(s) 538, 860, 872, 903
DdeI CTNAG 2 cut(s) 593, 617
DpnI GATC 3 cut(s) 18, 189, 708
DpnII GATC 3 cut(s) 16, 187, 706
DriI GACNNNNNGTC 1 cut(s) 454
Eam1105I GACNNNNNGTC 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 967
Eco130I CCWWGG 2 cut(s) 357, 685
Eco147I AGGCCT 1 cut(s) 591
Eco24I GRGCYC 3 cut(s) 675, 900, 969
Eco47I GGWCC 2 cut(s) 466, 953
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 2 cut(s) 273, 522
Eco88I CYCGRG 1 cut(s) 893
Eco91I GGTNACC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 967
EcoO109I RGGNCCY 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 2 cut(s) 357, 685
EcoT38I GRGCYC 3 cut(s) 675, 900, 969
ErhI CCWWGG 2 cut(s) 357, 685
FaeI CATG 8 cut(s) 23, 307, 341, 462, 648, 826, 918, 1024
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 8 cut(s) 19, 303, 337, 458, 644, 822, 914, 1020
FblI GTMKAC 3 cut(s) 133, 426, 887
Fnu4HI GCNGC 2 cut(s) 249, 344
FokI GGATG 4 cut(s) 20, 124, 270, 320
FriOI GRGCYC 3 cut(s) 675, 900, 969
Fsp4HI GCNGC 2 cut(s) 249, 344
FspBI CTAG 3 cut(s) 633, 686, 815
GluI GCNGC 2 cut(s) 249, 344
GsuI CTGGAG 3 cut(s) 47, 417, 463
HaeIII GGCC 2 cut(s) 591, 747
HapII CCGG 5 cut(s) 125, 567, 659, 668, 986
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 8 cut(s) 23, 307, 341, 462, 648, 826, 918, 1024
HincII GTYRAC 2 cut(s) 397, 888
HindII GTYRAC 2 cut(s) 397, 888
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 2 cut(s) 83, 280
HpaII CCGG 5 cut(s) 125, 567, 659, 668, 986
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 5 cut(s) 134, 397, 427, 860, 888
Hpy188I TCNGA 3 cut(s) 580, 706, 976
Hpy188III TCNNGA 7 cut(s) 20, 125, 164, 338, 385, 567, 853
Hpy8I GTNNAC 5 cut(s) 134, 397, 427, 860, 888
Hpy99I CGWCG 2 cut(s) 53, 935
HpyAV CCTTC 4 cut(s) 25, 385, 415, 547
HpyCH4III ACNGT 3 cut(s) 45, 885, 953
HpyCH4IV ACGT 4 cut(s) 300, 523, 905, 1027
HpyCH4V TGCA 7 cut(s) 172, 408, 462, 510, 733, 829, 914
HpyF3I CTNAG 2 cut(s) 593, 617
HpySE526I ACGT 4 cut(s) 300, 523, 905, 1027
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 8 cut(s) 23, 307, 341, 462, 648, 826, 918, 1024
Kpn2I TCCGGA 2 cut(s) 124, 566
Kzo9I GATC 3 cut(s) 16, 187, 706
LmnI GCTCC 4 cut(s) 105, 670, 797, 964
Lsp1109I GCAGC 2 cut(s) 260, 355
LweI GCATC 2 cut(s) 187, 867
MaeI CTAG 3 cut(s) 633, 686, 815
MaeII ACGT 4 cut(s) 300, 523, 905, 1027
MaeIII GTNAC 2 cut(s) 91, 233
MalI GATC 3 cut(s) 18, 189, 708
MboI GATC 3 cut(s) 16, 187, 706
MboII GAAGA 7 cut(s) 266, 394, 400, 485, 503, 749, 1010
MfeI CAATTG 1 cut(s) 909
MhlI GDGCHC 3 cut(s) 675, 900, 969
MluCI AATT 4 cut(s) 159, 273, 416, 909
MnlI CCTC 8 cut(s) 132, 248, 355, 479, 683, 787, 902, 947
MroI TCCGGA 2 cut(s) 124, 566
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 2 cut(s) 680, 959
MslI CAYNNNNRTG 1 cut(s) 643
MspA1I CMGCKG 2 cut(s) 251, 437
MspI CCGG 5 cut(s) 125, 567, 659, 668, 986
MspR9I CCNGG 3 cut(s) 350, 659, 668
MunI CAATTG 1 cut(s) 909
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 376
NciI CCSGG 2 cut(s) 659, 668
NdeII GATC 3 cut(s) 16, 187, 706
NlaIII CATG 8 cut(s) 23, 307, 341, 462, 648, 826, 918, 1024
NlaIV GGNNCC 3 cut(s) 672, 694, 928
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 3 cut(s) 648, 826, 918
PaeI GCATGC 1 cut(s) 648
PaeR7I CTCGAG 1 cut(s) 893
PagI TCATGA 2 cut(s) 19, 337
PceI AGGCCT 1 cut(s) 591
PciI ACATGT 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 83, 280
Pfl23II CGTACG 1 cut(s) 902
PkrI GCNGC 2 cut(s) 250, 345
Ppu21I YACGTR 1 cut(s) 524
PpuMI RGGWCCY 1 cut(s) 466
PscI ACATGT 1 cut(s) 822
PsiI TTATAA 1 cut(s) 1032
Psp124BI GAGCTC 1 cut(s) 969
Psp1406I AACGTT 1 cut(s) 1027
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 348
PspEI GGTNACC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 348
PspLI CGTACG 1 cut(s) 902
PspN4I GGNNCC 3 cut(s) 672, 694, 928
PspPI GGNCC 2 cut(s) 466, 953
PspPPI RGGWCCY 1 cut(s) 466
PspXI VCTCGAGB 1 cut(s) 893
PstI CTGCAG 1 cut(s) 512
PvuII CAGCTG 2 cut(s) 251, 437
RsaI GTAC 4 cut(s) 539, 861, 873, 904
RsaNI GTAC 4 cut(s) 538, 860, 872, 903
RseI CAYNNNNRTG 1 cut(s) 643
Rsr2I CGGWCCG 1 cut(s) 953
RsrII CGGWCCG 1 cut(s) 953
SacI GAGCTC 1 cut(s) 969
SalI GTCGAC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 680, 959
SatI GCNGC 2 cut(s) 249, 344
Sau3AI GATC 3 cut(s) 16, 187, 706
Sau96I GGNCC 2 cut(s) 466, 953
ScaI AGTACT 1 cut(s) 873
ScrFI CCNGG 3 cut(s) 350, 659, 668
SduI GDGCHC 3 cut(s) 675, 900, 969
SfaNI GCATC 2 cut(s) 187, 867
SfcI CTRYAG 1 cut(s) 508
Sfr274I CTCGAG 1 cut(s) 893
SinI GGWCC 2 cut(s) 466, 953
SlaI CTCGAG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 643
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
SphI GCATGC 1 cut(s) 648
Sse9I AATT 4 cut(s) 159, 273, 416, 909
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 3 cut(s) 200, 241, 331
SspI AATATT 1 cut(s) 769
SspMI CTAG 3 cut(s) 633, 686, 815
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 348, 657, 666
StyI CCWWGG 2 cut(s) 357, 685
TaaI ACNGT 3 cut(s) 45, 885, 953
TaiI ACGT 4 cut(s) 303, 526, 908, 1030
TaqI TCGA 5 cut(s) 48, 186, 471, 887, 894
TasI AATT 4 cut(s) 159, 273, 416, 909
TatI WGTACW 3 cut(s) 537, 859, 871
TfiI GAWTC 2 cut(s) 83, 280
Tru1I TTAA 2 cut(s) 680, 959
Tru9I TTAA 2 cut(s) 680, 959
TscAI CASTG 3 cut(s) 604, 795, 888
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 2 cut(s) 248, 343
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 5 cut(s) 326, 492, 548, 749, 1037
TspGWI ACGGA 2 cut(s) 530, 945
TspRI CASTG 3 cut(s) 604, 795, 888
VpaK11BI GGWCC 2 cut(s) 466, 953
XapI RAATTY 1 cut(s) 159
XceI RCATGY 3 cut(s) 648, 826, 918
XhoI CTCGAG 1 cut(s) 893
XmaJI CCTAGG 1 cut(s) 685
XmiI GTMKAC 3 cut(s) 133, 426, 887
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 3 cut(s) 633, 686, 815
ZraI GACGTC 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.