RchiOBHm_Chr6g0288651

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000837
Physical Location & Seq
Forward (+)
51846661 .. 51848917
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25897

Sequence Viewer

Length: 639 bp
ATGGGGTTCTTTCAGATAGTCAACCATGGAGTTCCACTTGCTGTTATGGAGGAGATGCTTCAAGGAATTTGTAGATTTCATGAGCAGCCTGCAGAAGACAAGATGGAGTTGTACTCTCGTGACTTCAAGAATCCGGTCAACTTTTATTGCAGTGGAGATCTGAAAGTGCGTACAAAATCAGCAGTAGACTGGAGAGACACCTTATTCTGCAGAGAAGTAGATGATGAATGGGATTTTGAAGCACTGCCTCAAGTCTGCAGCAAGTACGATCACCTGGCACACTTGGGCTACTTGAAATCTTTCTCATGTCATGCCATGCCACTATTATATATCCAATTTGCGTGCCCTGAGCTCGATTTGACTCTAGGCACAATAAAACATTCAGAACCCTCATTTCTTACCTTAGTTCTGCAGGACGAGATTGGGGGCCTCCAAGTTCTCCATAAAGATCAATTGGTTGATGTGCCACCAGTGAATGGAGCTTTTGTAGCAAATCTCGGTGACTTTGTGCAGCTGATTACTAATAACAAGTTCAATAGTGTGCAACATAGAGTGCTTGCTACCTCGCATGTCAAGCCACGAATATCAGTTGTGTCTTTCTTCGTTCCTATGAATGGTGACAAACGTGTCAGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

212

Amino Acids

24.22

Weight (kDa)

5.96

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 1 - 73 4.7e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 114 - 202 5.2e-24 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 626
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 66
AfaI GTAC 3 cut(s) 113, 172, 266
AfiI CCNNNNNNNGG 2 cut(s) 476, 614
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 5 cut(s) 62, 127, 239, 295, 535
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 3 cut(s) 352, 482, 514
AluI AGCT 3 cut(s) 352, 482, 514
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 189
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 3 cut(s) 85, 258, 511
ApoI RAATTY 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 263, 512, 629
BaeGI GKGCMC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 3 cut(s) 97, 270, 523
BccI CCATC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 4 cut(s) 90, 208, 256, 410
BglII AGATCT 1 cut(s) 157
BisI GCNGC 3 cut(s) 86, 259, 512
BlsI GCNGC 3 cut(s) 87, 260, 513
Bme1390I CCNGG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 428
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 102
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 211
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 25
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 614
Bse1I ACTGG 2 cut(s) 194, 470
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 25
BseLI CCNNNNNNNGG 2 cut(s) 476, 614
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 2 cut(s) 194, 470
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 3 cut(s) 97, 270, 523
BsgI GTGCAG 1 cut(s) 530
BshFI GGCC 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 476, 614
BsmAI GTCTC 1 cut(s) 189
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 347, 354
Bsp143I GATC 3 cut(s) 157, 268, 448
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 1 cut(s) 340
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 428
BspMAI CTGCAG 4 cut(s) 94, 212, 260, 414
BsrI ACTGG 2 cut(s) 194, 470
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 3 cut(s) 157, 268, 448
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 90, 343, 558
BstDEI CTNAG 2 cut(s) 348, 403
BstDSI CCRYGG 1 cut(s) 25
BstKTI GATC 3 cut(s) 160, 271, 451
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 3 cut(s) 157, 268, 448
BstMWI GCNNNNNNNGC 2 cut(s) 488, 574
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 273
BstSFI CTRYAG 4 cut(s) 90, 208, 256, 410
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 97, 270, 523
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 25
BtsI GCAGTG 2 cut(s) 157, 242
BtsIMutI CAGTG 3 cut(s) 157, 242, 477
Cac8I GCNNGC 3 cut(s) 90, 343, 558
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 3 cut(s) 112, 171, 265
CviAII CATG 6 cut(s) 26, 80, 306, 311, 316, 569
CviJI RGCY 7 cut(s) 88, 288, 352, 429, 482, 514, 577
CviKI_1 RGCY 7 cut(s) 88, 288, 352, 429, 482, 514, 577
CviQI GTAC 3 cut(s) 112, 171, 265
DdeI CTNAG 2 cut(s) 348, 403
DpnI GATC 3 cut(s) 159, 270, 450
DpnII GATC 3 cut(s) 157, 268, 448
DrdI GACNNNNNNGTC 1 cut(s) 626
DseDI GACNNNNNNGTC 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 25
FaeI CATG 6 cut(s) 29, 83, 309, 314, 319, 572
FatI CATG 6 cut(s) 25, 79, 305, 310, 315, 568
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 86, 259, 512
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 3 cut(s) 86, 259, 512
FspBI CTAG 1 cut(s) 365
GluI GCNGC 3 cut(s) 86, 259, 512
GsuI CTGGAG 1 cut(s) 211
HaeIII GGCC 1 cut(s) 429
HapII CCGG 1 cut(s) 134
Hin1II CATG 6 cut(s) 29, 83, 309, 314, 319, 572
HincII GTYRAC 2 cut(s) 22, 139
HindII GTYRAC 2 cut(s) 22, 139
HinfI GANTC 2 cut(s) 130, 361
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 263, 512, 629
Hpy166II GTNNAC 3 cut(s) 22, 139, 187
Hpy188I TCNGA 3 cut(s) 15, 162, 385
Hpy188III TCNNGA 3 cut(s) 80, 119, 127
Hpy8I GTNNAC 3 cut(s) 22, 139, 187
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 7 cut(s) 92, 150, 210, 258, 412, 511, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 574
HpyF3I CTNAG 2 cut(s) 348, 403
HpySE526I ACGT 1 cut(s) 625
Hsp92II CATG 6 cut(s) 29, 83, 309, 314, 319, 572
Kzo9I GATC 3 cut(s) 157, 268, 448
LmnI GCTCC 1 cut(s) 479
LpnPI CCDG 9 cut(s) 102, 147, 175, 260, 287, 360, 398, 483, 616
Lsp1109I GCAGC 3 cut(s) 97, 270, 523
LweI GCATC 1 cut(s) 45
MaeI CTAG 1 cut(s) 365
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 3 cut(s) 119, 500, 617
MalI GATC 3 cut(s) 159, 270, 450
MboI GATC 3 cut(s) 157, 268, 448
MboII GAAGA 2 cut(s) 107, 592
MfeI CAATTG 1 cut(s) 452
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 347, 354
MluCI AATT 3 cut(s) 66, 335, 452
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 5 cut(s) 43, 258, 400, 440, 574
MroXI GAANNNNTTC 1 cut(s) 299
MseI TTAA 1 cut(s) 637
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 275
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 488, 574
NcoI CCATGG 1 cut(s) 25
NdeII GATC 3 cut(s) 157, 268, 448
NlaIII CATG 6 cut(s) 29, 83, 309, 314, 319, 572
NlaIV GGNNCC 1 cut(s) 428
NmuCI GTSAC 3 cut(s) 119, 500, 617
NspI RCATGY 1 cut(s) 572
PagI TCATGA 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 631
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 130
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 3 cut(s) 87, 260, 513
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 428
PspPI GGNCC 1 cut(s) 427
PstI CTGCAG 4 cut(s) 94, 212, 260, 414
PsuI RGATCY 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 514
RsaI GTAC 3 cut(s) 113, 172, 266
RsaNI GTAC 3 cut(s) 112, 171, 265
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 637
SatI GCNGC 3 cut(s) 86, 259, 512
Sau3AI GATC 3 cut(s) 157, 268, 448
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 2 cut(s) 347, 354
SetI ASST 8 cut(s) 203, 276, 354, 404, 484, 516, 566, 628
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 4 cut(s) 90, 208, 256, 410
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 3 cut(s) 66, 335, 452
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 1 cut(s) 25
TaiI ACGT 1 cut(s) 628
TaqI TCGA 1 cut(s) 354
TasI AATT 3 cut(s) 66, 335, 452
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 637
Tru9I TTAA 1 cut(s) 637
TscAI CASTG 3 cut(s) 157, 249, 477
TseFI GTSAC 3 cut(s) 119, 500, 617
TseI GCWGC 3 cut(s) 85, 258, 511
Tsp45I GTSAC 3 cut(s) 119, 500, 617
TspDTI ATGAA 3 cut(s) 68, 240, 626
TspRI CASTG 3 cut(s) 157, 249, 477
Van91I CCANNNNNTGG 1 cut(s) 476
XapI RAATTY 1 cut(s) 66
XceI RCATGY 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 299
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.