FvH4_2g35541

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001602
Physical Location & Seq
Forward (+)
26175695 .. 26183943
8249 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35541.t1

Sequence Viewer

Length: 438 bp
ATGTCGGATGAAGAAACAGATTTGGAGGAGTCCTATGAAGAGCCACGCGATGTTGAGACTTTTGAGGATTGCTTAAACGAGGAGTCCATCAAAGCTCCTACATTTGAACCTTTATTGCTGTTACATGGAAGACCACATGATTCTTCACTCCATCACTCAAGCTCTACACATGTTCATTCTGTCCAAGCATCATGTGAATTTGTTGAGCATGTGGATATGGTGGTACATGAATGTCTCAAATTAGATAAGGAGATTCTTGTTGTGATTGTTAATAGTTGGAGACAATGGAAGAAGAGAAAGAAAAAGAGAAGCTTCATATCTTATATGTCCATTGCTTCTAAGTTACTCAAATTGCTACCAAAGGACCATGAAATATCTTTGAAGAGGGTCAAGCATTTGATCGAACCGCGGCCTAAGCCTCCTGATTATTCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.97

Weight (kDa)

6.05

Isoelectric Point (pI)

67.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 48, 409
AciI CCGC 2 cut(s) 407, 409
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 2 cut(s) 107, 382
AluBI AGCT 3 cut(s) 95, 162, 312
AluI AGCT 3 cut(s) 95, 162, 312
Alw26I GTCTC 3 cut(s) 50, 239, 274
AoxI GGCC 1 cut(s) 410
ApoI RAATTY 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 136
BccI CCATC 2 cut(s) 95, 159
BcoDI GTCTC 3 cut(s) 50, 239, 274
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 364
BmsI GCATC 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 407
BseGI GGATG 1 cut(s) 13
BseMI GCAATG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 41, 95
Bsh1236I CGCG 2 cut(s) 48, 409
BshFI GGCC 1 cut(s) 412
BsmAI GTCTC 3 cut(s) 50, 239, 274
BsnI GGCC 1 cut(s) 412
Bsp143I GATC 1 cut(s) 399
BspACI CCGC 2 cut(s) 407, 409
BspANI GGCC 1 cut(s) 412
BspFNI CGCG 2 cut(s) 48, 409
BspQI GCTCTTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 407
BssMI GATC 1 cut(s) 399
Bst6I CTCTTC 3 cut(s) 33, 287, 377
BstDEI CTNAG 2 cut(s) 339, 414
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 1 cut(s) 13
BstFNI CGCG 2 cut(s) 48, 409
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 3 cut(s) 50, 239, 274
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNSI RCATGY 2 cut(s) 173, 212
BstUI CGCG 2 cut(s) 48, 409
BstV2I GAAGAC 1 cut(s) 136
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 364
Cfr42I CCGCGG 1 cut(s) 410
Csp6I GTAC 1 cut(s) 224
CviAII CATG 7 cut(s) 125, 137, 170, 192, 209, 227, 368
CviJI RGCY 6 cut(s) 43, 95, 162, 312, 412, 418
CviKI_1 RGCY 6 cut(s) 43, 95, 162, 312, 412, 418
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 2 cut(s) 339, 414
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 33, 287, 377
EarI CTCTTC 3 cut(s) 33, 287, 377
Eco47I GGWCC 1 cut(s) 364
FaeI CATG 7 cut(s) 128, 140, 173, 195, 212, 230, 371
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 7 cut(s) 124, 136, 169, 191, 208, 226, 367
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 1 cut(s) 410
GluI GCNGC 1 cut(s) 410
HaeIII GGCC 1 cut(s) 412
Hin1II CATG 7 cut(s) 128, 140, 173, 195, 212, 230, 371
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 4 cut(s) 29, 83, 140, 253
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 2 cut(s) 339, 414
Hsp92II CATG 7 cut(s) 128, 140, 173, 195, 212, 230, 371
KspI CCGCGG 1 cut(s) 410
Kzo9I GATC 1 cut(s) 399
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 1 cut(s) 100
LweI GCATC 1 cut(s) 197
MaeIII GTNAC 2 cut(s) 120, 342
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MboII GAAGA 7 cut(s) 23, 50, 135, 141, 301, 304, 394
MluCI AATT 3 cut(s) 197, 239, 350
MlyI GAGTC 2 cut(s) 38, 92
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 5 cut(s) 19, 58, 73, 378, 429
MseI TTAA 2 cut(s) 74, 270
MspA1I CMGCKG 1 cut(s) 409
MvnI CGCG 2 cut(s) 48, 409
MwoI GCNNNNNNNGC 1 cut(s) 415
NdeII GATC 1 cut(s) 399
NlaIII CATG 7 cut(s) 128, 140, 173, 195, 212, 230, 371
NspI RCATGY 2 cut(s) 173, 212
PciI ACATGT 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 140, 253
PkrI GCNGC 1 cut(s) 411
PleI GAGTC 2 cut(s) 37, 91
PpsI GAGTC 2 cut(s) 37, 91
PscI ACATGT 1 cut(s) 169
PspPI GGNCC 1 cut(s) 364
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SacII CCGCGG 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 74, 270
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 2 cut(s) 38, 92
SetI ASST 4 cut(s) 97, 112, 164, 314
SfaNI GCATC 1 cut(s) 197
Sfr303I CCGCGG 1 cut(s) 410
SgrBI CCGCGG 1 cut(s) 410
SinI GGWCC 1 cut(s) 364
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 3 cut(s) 197, 239, 350
SsiI CCGC 2 cut(s) 407, 409
TaqI TCGA 1 cut(s) 402
TasI AATT 3 cut(s) 197, 239, 350
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 140, 253
Tru1I TTAA 2 cut(s) 74, 270
Tru9I TTAA 2 cut(s) 74, 270
TspDTI ATGAA 6 cut(s) 24, 51, 164, 243, 304, 384
VpaK11BI GGWCC 1 cut(s) 364
XapI RAATTY 1 cut(s) 197
XceI RCATGY 2 cut(s) 173, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.