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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 67 |
| AciI | CCGC | 1 cut(s) | 236 |
| AclWI | GGATC | 4 cut(s) | 5, 18, 107, 179 |
| AfaI | GTAC | 2 cut(s) | 168, 216 |
| AgsI | TTSAA | 2 cut(s) | 79, 313 |
| AlwI | GGATC | 4 cut(s) | 5, 18, 107, 179 |
| Aor13HI | TCCGGA | 1 cut(s) | 67 |
| AseI | ATTAAT | 1 cut(s) | 303 |
| BamHI | GGATCC | 1 cut(s) | 10 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 150, 182 |
| BceAI | ACGGC | 1 cut(s) | 251 |
| BmiI | GGNNCC | 1 cut(s) | 12 |
| BpuEI | CTTGAG | 2 cut(s) | 4, 224 |
| BsaBI | GATNNNNATC | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 1 cut(s) | 267 |
| BsaWI | WCCGGW | 1 cut(s) | 67 |
| Bse1I | ACTGG | 1 cut(s) | 170 |
| Bse8I | GATNNNNATC | 1 cut(s) | 117 |
| BseAI | TCCGGA | 1 cut(s) | 67 |
| BseDI | CCNNGG | 1 cut(s) | 267 |
| BseJI | GATNNNNATC | 1 cut(s) | 117 |
| BseNI | ACTGG | 1 cut(s) | 170 |
| BsiSI | CCGG | 1 cut(s) | 68 |
| BslFI | GGGAC | 1 cut(s) | 86 |
| BsmFI | GGGAC | 1 cut(s) | 86 |
| Bsp13I | TCCGGA | 1 cut(s) | 67 |
| Bsp1407I | TGTACA | 1 cut(s) | 214 |
| Bsp143I | GATC | 4 cut(s) | 10, 112, 160, 184 |
| BspACI | CCGC | 1 cut(s) | 236 |
| BspEI | TCCGGA | 1 cut(s) | 67 |
| BspLI | GGNNCC | 1 cut(s) | 12 |
| BspPI | GGATC | 4 cut(s) | 5, 18, 107, 179 |
| BsrGI | TGTACA | 1 cut(s) | 214 |
| BsrI | ACTGG | 1 cut(s) | 170 |
| BssECI | CCNNGG | 1 cut(s) | 267 |
| BssMI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| Bst6I | CTCTTC | 1 cut(s) | 61 |
| BstAUI | TGTACA | 1 cut(s) | 214 |
| BstDSI | CCRYGG | 1 cut(s) | 267 |
| BstKTI | GATC | 4 cut(s) | 13, 115, 163, 187 |
| BstMBI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 233 |
| BstX2I | RGATCY | 1 cut(s) | 10 |
| BstYI | RGATCY | 1 cut(s) | 10 |
| BtgI | CCRYGG | 1 cut(s) | 267 |
| Csp6I | GTAC | 2 cut(s) | 167, 215 |
| CviAII | CATG | 3 cut(s) | 60, 84, 142 |
| CviJI | RGCY | 2 cut(s) | 196, 266 |
| CviKI_1 | RGCY | 2 cut(s) | 196, 266 |
| CviQI | GTAC | 2 cut(s) | 167, 215 |
| DpnI | GATC | 4 cut(s) | 12, 114, 162, 186 |
| DpnII | GATC | 4 cut(s) | 10, 112, 160, 184 |
| Eam1104I | CTCTTC | 1 cut(s) | 61 |
| EarI | CTCTTC | 1 cut(s) | 61 |
| FaeI | CATG | 3 cut(s) | 63, 87, 145 |
| FaiI | YATR | 8 cut(s) | 61, 85, 107, 111, 143, 154, 257, 288 |
| FaqI | GGGAC | 1 cut(s) | 86 |
| FatI | CATG | 3 cut(s) | 59, 83, 141 |
| HapII | CCGG | 1 cut(s) | 68 |
| Hin1II | CATG | 3 cut(s) | 63, 87, 145 |
| HinfI | GANTC | 3 cut(s) | 64, 134, 242 |
| HpaII | CCGG | 1 cut(s) | 68 |
| Hpy188III | TCNNGA | 1 cut(s) | 68 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 233 |
| Hsp92II | CATG | 3 cut(s) | 63, 87, 145 |
| Kpn2I | TCCGGA | 1 cut(s) | 67 |
| Kzo9I | GATC | 4 cut(s) | 10, 112, 160, 184 |
| LpnPI | CCDG | 3 cut(s) | 81, 183, 305 |
| MalI | GATC | 4 cut(s) | 12, 114, 162, 186 |
| MboI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| MboII | GAAGA | 3 cut(s) | 48, 170, 251 |
| MfeI | CAATTG | 1 cut(s) | 210 |
| MflI | RGATCY | 1 cut(s) | 10 |
| MluCI | AATT | 3 cut(s) | 210, 297, 304 |
| MmeI | TCCRAC | 1 cut(s) | 20 |
| MnlI | CCTC | 3 cut(s) | 185, 199, 208 |
| MroI | TCCGGA | 1 cut(s) | 67 |
| MseI | TTAA | 1 cut(s) | 303 |
| MslI | CAYNNNNRTG | 1 cut(s) | 142 |
| MspI | CCGG | 1 cut(s) | 68 |
| MunI | CAATTG | 1 cut(s) | 210 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 233 |
| NdeII | GATC | 4 cut(s) | 10, 112, 160, 184 |
| NlaIII | CATG | 3 cut(s) | 63, 87, 145 |
| NlaIV | GGNNCC | 1 cut(s) | 12 |
| PfeI | GAWTC | 3 cut(s) | 64, 134, 242 |
| PshBI | ATTAAT | 1 cut(s) | 303 |
| PspN4I | GGNNCC | 1 cut(s) | 12 |
| PsuI | RGATCY | 1 cut(s) | 10 |
| RsaI | GTAC | 2 cut(s) | 168, 216 |
| RsaNI | GTAC | 2 cut(s) | 167, 215 |
| RseI | CAYNNNNRTG | 1 cut(s) | 142 |
| SaqAI | TTAA | 1 cut(s) | 303 |
| Sau3AI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| SetI | ASST | 1 cut(s) | 94 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 142 |
| SmlI | CTYRAG | 2 cut(s) | 19, 203 |
| SmoI | CTYRAG | 2 cut(s) | 19, 203 |
| Sse9I | AATT | 3 cut(s) | 210, 297, 304 |
| SsiI | CCGC | 1 cut(s) | 236 |
| SspI | AATATT | 2 cut(s) | 130, 277 |
| TasI | AATT | 3 cut(s) | 210, 297, 304 |
| TatI | WGTACW | 1 cut(s) | 214 |
| TfiI | GAWTC | 3 cut(s) | 64, 134, 242 |
| Tru1I | TTAA | 1 cut(s) | 303 |
| Tru9I | TTAA | 1 cut(s) | 303 |
| TspDTI | ATGAA | 4 cut(s) | 48, 126, 130, 206 |
| VspI | ATTAAT | 1 cut(s) | 303 |