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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 331 |
| AciI | CCGC | 1 cut(s) | 331 |
| AclWI | GGATC | 3 cut(s) | 5, 107, 179 |
| AcoI | YGGCCR | 1 cut(s) | 146 |
| AfaI | GTAC | 1 cut(s) | 216 |
| AgsI | TTSAA | 2 cut(s) | 79, 313 |
| AluBI | AGCT | 1 cut(s) | 266 |
| AluI | AGCT | 1 cut(s) | 266 |
| AlwI | GGATC | 3 cut(s) | 5, 107, 179 |
| AoxI | GGCC | 1 cut(s) | 146 |
| ApeKI | GCWGC | 1 cut(s) | 88 |
| AseI | ATTAAT | 1 cut(s) | 303 |
| BbvI | GCAGC | 1 cut(s) | 100 |
| BccI | CCATC | 1 cut(s) | 129 |
| BceAI | ACGGC | 1 cut(s) | 133 |
| BisI | GCNGC | 1 cut(s) | 89 |
| BlsI | GCNGC | 1 cut(s) | 90 |
| BpuEI | CTTGAG | 2 cut(s) | 4, 224 |
| BseRI | GAGGAG | 1 cut(s) | 341 |
| BseXI | GCAGC | 1 cut(s) | 100 |
| BshFI | GGCC | 1 cut(s) | 148 |
| BslFI | GGGAC | 2 cut(s) | 20, 86 |
| BsmFI | GGGAC | 2 cut(s) | 20, 86 |
| BsnI | GGCC | 1 cut(s) | 148 |
| Bsp1407I | TGTACA | 1 cut(s) | 214 |
| Bsp143I | GATC | 4 cut(s) | 10, 112, 160, 184 |
| BspACI | CCGC | 1 cut(s) | 331 |
| BspANI | GGCC | 1 cut(s) | 148 |
| BspPI | GGATC | 3 cut(s) | 5, 107, 179 |
| BsrBI | CCGCTC | 1 cut(s) | 331 |
| BsrGI | TGTACA | 1 cut(s) | 214 |
| BssMI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| Bst6I | CTCTTC | 1 cut(s) | 61 |
| BstAUI | TGTACA | 1 cut(s) | 214 |
| BstKTI | GATC | 4 cut(s) | 13, 115, 163, 187 |
| BstMBI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| BstV1I | GCAGC | 1 cut(s) | 100 |
| BsuRI | GGCC | 1 cut(s) | 148 |
| Csp6I | GTAC | 1 cut(s) | 215 |
| CviAII | CATG | 3 cut(s) | 60, 84, 142 |
| CviJI | RGCY | 4 cut(s) | 91, 148, 196, 266 |
| CviKI_1 | RGCY | 4 cut(s) | 91, 148, 196, 266 |
| CviQI | GTAC | 1 cut(s) | 215 |
| DpnI | GATC | 4 cut(s) | 12, 114, 162, 186 |
| DpnII | GATC | 4 cut(s) | 10, 112, 160, 184 |
| EaeI | YGGCCR | 1 cut(s) | 146 |
| Eam1104I | CTCTTC | 1 cut(s) | 61 |
| EarI | CTCTTC | 1 cut(s) | 61 |
| FaeI | CATG | 3 cut(s) | 63, 87, 145 |
| FaiI | YATR | 9 cut(s) | 61, 85, 107, 111, 143, 154, 257, 288, 340 |
| FaqI | GGGAC | 2 cut(s) | 20, 86 |
| FatI | CATG | 3 cut(s) | 59, 83, 141 |
| FauI | CCCGC | 1 cut(s) | 324 |
| Fnu4HI | GCNGC | 1 cut(s) | 89 |
| Fsp4HI | GCNGC | 1 cut(s) | 89 |
| GluI | GCNGC | 1 cut(s) | 89 |
| HaeIII | GGCC | 1 cut(s) | 148 |
| Hin1II | CATG | 3 cut(s) | 63, 87, 145 |
| HinfI | GANTC | 3 cut(s) | 64, 134, 242 |
| Hpy188III | TCNNGA | 2 cut(s) | 68, 203 |
| Hsp92II | CATG | 3 cut(s) | 63, 87, 145 |
| Kzo9I | GATC | 4 cut(s) | 10, 112, 160, 184 |
| LmnI | GCTCC | 1 cut(s) | 328 |
| LpnPI | CCDG | 2 cut(s) | 81, 305 |
| Lsp1109I | GCAGC | 1 cut(s) | 100 |
| MalI | GATC | 4 cut(s) | 12, 114, 162, 186 |
| MbiI | CCGCTC | 1 cut(s) | 331 |
| MboI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| MboII | GAAGA | 1 cut(s) | 48 |
| MfeI | CAATTG | 1 cut(s) | 210 |
| MluCI | AATT | 3 cut(s) | 210, 297, 304 |
| MmeI | TCCRAC | 1 cut(s) | 20 |
| MnlI | CCTC | 4 cut(s) | 185, 199, 208, 319 |
| MseI | TTAA | 1 cut(s) | 303 |
| MslI | CAYNNNNRTG | 1 cut(s) | 142 |
| MunI | CAATTG | 1 cut(s) | 210 |
| NdeII | GATC | 4 cut(s) | 10, 112, 160, 184 |
| NlaIII | CATG | 3 cut(s) | 63, 87, 145 |
| PfeI | GAWTC | 3 cut(s) | 64, 134, 242 |
| PkrI | GCNGC | 1 cut(s) | 90 |
| PshBI | ATTAAT | 1 cut(s) | 303 |
| RsaI | GTAC | 1 cut(s) | 216 |
| RsaNI | GTAC | 1 cut(s) | 215 |
| RseI | CAYNNNNRTG | 1 cut(s) | 142 |
| SaqAI | TTAA | 1 cut(s) | 303 |
| SatI | GCNGC | 1 cut(s) | 89 |
| Sau3AI | GATC | 4 cut(s) | 10, 112, 160, 184 |
| SetI | ASST | 1 cut(s) | 268 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 142 |
| SmlI | CTYRAG | 2 cut(s) | 19, 203 |
| SmoI | CTYRAG | 2 cut(s) | 19, 203 |
| Sse9I | AATT | 3 cut(s) | 210, 297, 304 |
| SsiI | CCGC | 1 cut(s) | 331 |
| SspI | AATATT | 2 cut(s) | 130, 277 |
| TasI | AATT | 3 cut(s) | 210, 297, 304 |
| TatI | WGTACW | 1 cut(s) | 214 |
| TfiI | GAWTC | 3 cut(s) | 64, 134, 242 |
| Tru1I | TTAA | 1 cut(s) | 303 |
| Tru9I | TTAA | 1 cut(s) | 303 |
| TseI | GCWGC | 1 cut(s) | 88 |
| TspDTI | ATGAA | 4 cut(s) | 48, 126, 130, 206 |
| VspI | ATTAAT | 1 cut(s) | 303 |