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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 8, 21 |
| AgsI | TTSAA | 2 cut(s) | 76, 130 |
| AluBI | AGCT | 3 cut(s) | 98, 214, 248 |
| AluI | AGCT | 3 cut(s) | 98, 214, 248 |
| AlwI | GGATC | 2 cut(s) | 8, 21 |
| ApeKI | GCWGC | 1 cut(s) | 85 |
| BamHI | GGATCC | 1 cut(s) | 13 |
| BbvI | GCAGC | 1 cut(s) | 97 |
| BccI | CCATC | 2 cut(s) | 36, 353 |
| BfaI | CTAG | 1 cut(s) | 385 |
| BfmI | CTRYAG | 1 cut(s) | 357 |
| BisI | GCNGC | 1 cut(s) | 86 |
| BlsI | GCNGC | 1 cut(s) | 87 |
| BmiI | GGNNCC | 1 cut(s) | 15 |
| BpuEI | CTTGAG | 1 cut(s) | 7 |
| BsaBI | GATNNNNATC | 1 cut(s) | 129 |
| BsaJI | CCNNGG | 1 cut(s) | 267 |
| Bse8I | GATNNNNATC | 1 cut(s) | 129 |
| BseDI | CCNNGG | 1 cut(s) | 267 |
| BseGI | GGATG | 2 cut(s) | 47, 373 |
| BseJI | GATNNNNATC | 1 cut(s) | 129 |
| BseRI | GAGGAG | 1 cut(s) | 242 |
| BseXI | GCAGC | 1 cut(s) | 97 |
| BslFI | GGGAC | 1 cut(s) | 338 |
| BsmFI | GGGAC | 1 cut(s) | 338 |
| Bsp143I | GATC | 1 cut(s) | 13 |
| BspHI | TCATGA | 2 cut(s) | 121, 289 |
| BspLI | GGNNCC | 1 cut(s) | 15 |
| BspPI | GGATC | 2 cut(s) | 8, 21 |
| BspQI | GCTCTTC | 2 cut(s) | 77, 288 |
| BssECI | CCNNGG | 1 cut(s) | 267 |
| BssMI | GATC | 1 cut(s) | 13 |
| BssT1I | CCWWGG | 1 cut(s) | 267 |
| Bst6I | CTCTTC | 4 cut(s) | 67, 77, 288, 330 |
| BstC8I | GCNNGC | 1 cut(s) | 162 |
| BstDEI | CTNAG | 1 cut(s) | 331 |
| BstF5I | GGATG | 2 cut(s) | 47, 373 |
| BstKTI | GATC | 1 cut(s) | 16 |
| BstMBI | GATC | 1 cut(s) | 13 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 272 |
| BstNSI | RCATGY | 1 cut(s) | 380 |
| BstSFI | CTRYAG | 1 cut(s) | 357 |
| BstV1I | GCAGC | 1 cut(s) | 97 |
| BstX2I | RGATCY | 1 cut(s) | 13 |
| BstYI | RGATCY | 1 cut(s) | 13 |
| BtsCI | GGATG | 2 cut(s) | 47, 373 |
| Cac8I | GCNNGC | 1 cut(s) | 162 |
| CciI | TCATGA | 2 cut(s) | 121, 289 |
| CseI | GACGC | 1 cut(s) | 16 |
| CviAII | CATG | 9 cut(s) | 6, 66, 81, 122, 139, 175, 218, 290, 377 |
| CviJI | RGCY | 7 cut(s) | 70, 88, 98, 214, 248, 256, 266 |
| CviKI_1 | RGCY | 7 cut(s) | 70, 88, 98, 214, 248, 256, 266 |
| DdeI | CTNAG | 1 cut(s) | 331 |
| DpnI | GATC | 1 cut(s) | 15 |
| DpnII | GATC | 1 cut(s) | 13 |
| Eam1104I | CTCTTC | 4 cut(s) | 67, 77, 288, 330 |
| EarI | CTCTTC | 4 cut(s) | 67, 77, 288, 330 |
| Eco130I | CCWWGG | 1 cut(s) | 267 |
| EcoT14I | CCWWGG | 1 cut(s) | 267 |
| ErhI | CCWWGG | 1 cut(s) | 267 |
| FaeI | CATG | 9 cut(s) | 9, 69, 84, 125, 142, 178, 221, 293, 380 |
| FaqI | GGGAC | 1 cut(s) | 338 |
| FatI | CATG | 9 cut(s) | 5, 65, 80, 121, 138, 174, 217, 289, 376 |
| Fnu4HI | GCNGC | 1 cut(s) | 86 |
| FokI | GGATG | 2 cut(s) | 54, 380 |
| Fsp4HI | GCNGC | 1 cut(s) | 86 |
| FspBI | CTAG | 1 cut(s) | 385 |
| GluI | GCNGC | 1 cut(s) | 86 |
| HgaI | GACGC | 1 cut(s) | 16 |
| Hin1II | CATG | 9 cut(s) | 9, 69, 84, 125, 142, 178, 221, 293, 380 |
| HinfI | GANTC | 1 cut(s) | 341 |
| Hpy188III | TCNNGA | 4 cut(s) | 122, 155, 209, 290 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 272 |
| HpyF3I | CTNAG | 1 cut(s) | 331 |
| Hsp92II | CATG | 9 cut(s) | 9, 69, 84, 125, 142, 178, 221, 293, 380 |
| Kzo9I | GATC | 1 cut(s) | 13 |
| LguI | GCTCTTC | 2 cut(s) | 77, 288 |
| LmnI | GCTCC | 1 cut(s) | 178 |
| Lsp1109I | GCAGC | 1 cut(s) | 97 |
| MaeI | CTAG | 1 cut(s) | 385 |
| MalI | GATC | 1 cut(s) | 15 |
| MboI | GATC | 1 cut(s) | 13 |
| MboII | GAAGA | 6 cut(s) | 54, 64, 245, 305, 335, 347 |
| MflI | RGATCY | 1 cut(s) | 13 |
| MlyI | GAGTC | 1 cut(s) | 335 |
| MnlI | CCTC | 6 cut(s) | 4, 220, 223, 297, 331, 358 |
| MslI | CAYNNNNRTG | 1 cut(s) | 222 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 272 |
| NdeII | GATC | 1 cut(s) | 13 |
| NlaIII | CATG | 9 cut(s) | 9, 69, 84, 125, 142, 178, 221, 293, 380 |
| NlaIV | GGNNCC | 1 cut(s) | 15 |
| NspI | RCATGY | 1 cut(s) | 380 |
| PagI | TCATGA | 2 cut(s) | 121, 289 |
| PciSI | GCTCTTC | 2 cut(s) | 77, 288 |
| PkrI | GCNGC | 1 cut(s) | 87 |
| PleI | GAGTC | 1 cut(s) | 335 |
| PpsI | GAGTC | 1 cut(s) | 335 |
| PspN4I | GGNNCC | 1 cut(s) | 15 |
| PsuI | RGATCY | 1 cut(s) | 13 |
| RseI | CAYNNNNRTG | 1 cut(s) | 222 |
| SapI | GCTCTTC | 2 cut(s) | 77, 288 |
| SatI | GCNGC | 1 cut(s) | 86 |
| Sau3AI | GATC | 1 cut(s) | 13 |
| SchI | GAGTC | 1 cut(s) | 335 |
| SetI | ASST | 3 cut(s) | 100, 216, 250 |
| SfcI | CTRYAG | 1 cut(s) | 357 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 222 |
| SmlI | CTYRAG | 1 cut(s) | 22 |
| SmoI | CTYRAG | 1 cut(s) | 22 |
| SspMI | CTAG | 1 cut(s) | 385 |
| StyI | CCWWGG | 1 cut(s) | 267 |
| TseI | GCWGC | 1 cut(s) | 85 |
| TspDTI | ATGAA | 5 cut(s) | 54, 119, 127, 206, 306 |
| TspGWI | ACGGA | 1 cut(s) | 123 |
| XceI | RCATGY | 1 cut(s) | 380 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 63 |
| XspI | CTAG | 1 cut(s) | 385 |