FvH4_3g30933

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000756
Physical Location & Seq
Forward (+)
24552775 .. 24557304
4530 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30933.t1

Sequence Viewer

Length: 4530 bp
ATGGGTAAGAACAAGGTAGCTGGTGCAAATCAGCTCGCAACAGGTTCTCAGAGTGGTGATTTACCCACTTTGGAGACTGAGCTTCACAATCACAAGGAGCACACTGCTGCGCAGATCGGGGAGCTTCAGACCACTCTTAATGTGTATCAGGAGTCCTTGAATGCTTTCCGTACTGAGATGATGGCTGAGTTTCGAGCCTTGAGGGAGTCTACAGTGGCACCAGTGCAACCGGTGAGCTCTACACCTCTCAGGCTTGGATCTGCTCTCCCTGAAGGTGGAGCTACTGTTTTGGGGGCTTTGCAGGACTCTGCTGGTTCCACTCATGCTATGGCGAGCTCAGGGACTAGTTCACCGGGGGGTAACAGTTCTCAAAACCCACCTTTGGTCAATACTAGTGTTGGGGATGTTGAGCCATTGTTGCAGTTTAACACTAGTCAGCCCATTATTACAAATGATTCTGGGGTGGTTATGAATACCCTTACAGTGACCAAGGTGACTACACCAAAACCAGTGTTGGATAAGGGAAAGAGAGTCAGTTTGGAGAAAGGTCAAACGAGTGGAAGCAAGTCCCAAGTGAATGGATCGGCTAGACCACATCCTAACCAGATTTTTCAGAATGCCAATTTTGGAAACCAACCACCACCATATACACCAAATTGTGCAAATTTTTCACAAGATTTTGTGCAATCCCAAACCGCCATGCCAAATTTTGCTCAAAACCAGTTTGCCCCAAATTTTCCTCAAACCAGTTTTGTCCAACCATACACACATACCAGTTTTGTCCAACCTTATACACAAACCAGTTTTGCCCATACCTATACACCAGCTTCTTTTGGTACTCATATTCCACAGCCACAAACCATACCATATCCAAAATATACCATGGTTTCCACAACCACGGCTAGTCCATACCATAGTAACCAGATGAGTGCTAATACATACCCAAATATGCAGCCTTATGTAGCACAACAGTATCATCAGAATCCATATGACCCCAGTTTACCCACAATGAAACAGATGAAGTTAGAATGGAGTACTTTTGGAGGAGGAGATCCGGTTGAGTGGCTGAATAAAGCTGAACAGATTTTTGCATTCTACCAAATCCCTGAGGACCGAAAGCTAACTCTTGCCACATTGCACTTGACAGAGAAGGCCTCGGATAGGTGGTTCATGTTCAAACATGAATTCCCTAATTCATGGGCAGGGTTAGCAGAATTACTTATGAGAGAGTTTGGTAGTCATCGAAGAGGGGATTATCAGACTGCTTTGGCTAGAATGGAGCAATCAGGCTCGGTTGAGAGCTACATGGAAGAATTCACCAAGCTATCAAGGAGGTCTCATGGTTTCTCTTCCCAGAATCTGTTGTCATTCTTCATTGAAGGGTTGAAAGAAACCATACATTCTGATGTGAGAGCTCTAAAACCAACTTCTTTATATGAGGCATGTGAGCTGGCCAAGATCTATGAAGAGAAAGAGATGAAAACAAGAGGTCATTTTCAAGTTATGACTCAGGCCAGACCACCAGTGGCACCTCTGAAACAAGCATACAATTACAACCACAATGCCAACCTTCAGAAAGTTCAACAACCTGCAAGATTGGCACAACCGCAAGGGGGTATTATGGGAGGCCTTAATAAGAGATTGACACAAGTTGAGTACCAAGAGAGAAGGGCGAGAAACCAGTGTTTTTTCTGTGATGAAATCTTTAGGCCTGGACATAATTGCAGGAAGGGTCAAGCTTTGATGGTGATTGAGGTGGTACAAGAAGAGGATGAGGTTGAGGTGCCGGTGGAACAAATGACGGCAGGGATGGAAATTGACCAGGGAGTGGAGTTGAATGCTATAGAAGGAGTTGAATTGCAGCTACATGCTGTGGGAGATTGTATCAATAGTCAAACAATGCAGCTAAAGGGTGAATGTCACACACACCAGGTGCATGTGTTGGTAGATTCAGGAGCTTCCCATAATTTCATCCATCCAACAGTTGTCAAACGAGCCAAGGTGGTGATTCAACCAATCAAGCCACTTAGGGTGAGACTGGCCAGTGGTGATGTCATGGAGACAAAAGGGATGGCGCAGGTAACAATCAAACTCCAACAATATGCTCTTACTACTGCATATTACATCCTACCTATTTCAGGTTGTGAGGTAATTCTAGGGGCTCAGTGGTTGAAAAGTCTTGGAGACATAGTGTGGAATTTTGAAACAATGTGCATGAAGTTTATTCAGCAGGGTCATCAATATTTGCTTCAGGGGGAAACTGAATTGCAAGCTAGCATGGTCAGTTGCAAATCCATGACTAGGCTGTTGCGAAAGGAGCGAGAAGTTATGTTGATTCAACTGAATTCTGCACATCATGAGCCCCAAACACTAAAAACAACTGAAATACACCCTCTGATTAACCAAGTCTTACAGAAGTATCCCCAAGTTTTTGACACCCCTAACCAGTTGCCACCAGCCAGAAATCAAGACCACAAAATCGAGTTACTTCCCAACACCCCTCCTGTGAATGTGAGACCTTACAGATACCCCCATTTTCAAAAAACTGAAATTGAAAAAATCATCCAAGATTTGTTGGAGAATGGAGTGATAAGACCAAGTGTTAGCCCTTTTTCATCCCCAGTTTTGTTGGTAAAAAAGAAAGATGGTTCGTGGCGGTTATGTGTAGACTACAGGGCTCTTAATGCTGTCACTGTGAAGGACAAGTACCCAATTCCGATGGTAGATGAGCTAATCGATGAGTTACATGGGGCTGTGATCTTTACTAAGCTGGACTTGAGGTCTGGTTATTACCAAATCAGGATGCATGGGGAGGCTGTGGCTAAGACTGCTTTTCGGACACATTCAGGACATTATGAGTTTTTAGTGATGCCCTTTGGACTCACTAATGCCCCTTCAACATTTCAGGCTGTTATGAATGATGTTTTGAGACCTTGCTTGAGAAAATGCACCCTTGTTTTTATGGATGACATCCTGGTGTACAGTCCTTTTGTGGAAACTCATGTGGAGCACTTAGATTTGGTGTTATCCAAATTGCAGCAGCATTCATTGAAAGTGAAAGAAAGTAAGTGTGAGTTTGGGACAGACAAAGTAGAATACTTGGGACATGTGATCAGTGCTGCCGGAGTGGCTGTAGATCCGGTCAAGATTGAGTGCATCAAACTGTGGGAACAACCAAAAACTCTTAAGCAACTGAGGGGGTTTTTGGGATTGGCTGGATATTATAGAAAGTTTGTTAAGGGTTTTGGCATTATAGCTAAACCTCTCACTGACATGCTTAAGAAAGGTGGATTCAAGTGGACCCAGGCTGCAGTCATAGCATTTGAAGAACTGAAGGAGGCTCTAACATCCACACCAGTGCTAGCAATTCCAGATTTTACAAAAGAATTTGTTGTGGAAACTGATGCTTCAGATGTAGGCATTGGAGCAGTATTGTCCCAAGAAGGTCATCCAATTGCCTTCATGAGCAAGGCATTAGCTCAAAAACACTTGGCTCTATCGGTTTACGACAAGGAGATGTTGGCAGTGGTATCAGCTGTACAACAGTGGAGGCCTTATCTCCTGGGGCATCATTTCAAGATTCTGACTGACCACAGAACCATTGAGTACTTCTTAAAGCAGAAAATCACTACACCAGCTCAACAAAAGTGGTTAATCAAGTTAATGGGATATGATTATACCATTCAATACAAAGCTGGAAAGAACAATGCAGGTCCTGATTCCTTATCAAGAAGGCCTTCGCTGGCTGTGATCATGGGAATATCAAAACCTGTGCAGGAGTATGTCAATGAGTTAAAACAGGCATGTCTCAACCATGGAGAAACAGCCATAATTGTGAGATTATTGCAGCAAGGAGGGGAGAAGAAACACTACAAGCTGGTAAACTCTTTGCTCTACTACAAAGATAGGATCTTTGTGCCTCCAGTTGATGAATGGCGTAGTAAGGTGATTGAGGAGCTACATGGAGGGAAGTTGGGAGGTCATGCTGGGAGAGCTAGGACATACAAGAGGGTCACAAGAAATTTCAGGTGGCCAGGGGTGCAGAAAAGTGTTAAGGAGTTTGTAGCTTTCTGTGATGTGTGTCAACAACAACATGCAGAAACAGTCATGCCACCTGGGCTCTTGCAACCGGTAAGCATACCTCCACAAGCGTGGCACACAATCTCCATGGATTTCATAGAAGCTTTGCCAAAGTCAGAAGGAAAAACAGTGATCTGGGTCATCATTGACAAACTAACAAAGTATGCTCACTTCATTCCTTTGTCTCATCCATATTCAGCTGCAACATTGGTTAAGGTTTTTGTGCAAGAGGTAGCCAAACTGCATGGAATGCCAGTTAACATCATCTCTGATAGAGATCCCATATTCTTAAGTCTTTTTTGGCAAGCATTCTTTGAGCTTCAAGGAACTCAGTTAAGCAGATCTTCTGCTTACCACCCACAATCAGATGGCCAGACTGAGAACCTCAACAAGACCTTGGAACAGTACTTGAGATGTGTTATTGGGGAGAAACCCCACAGCTGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1510

Amino Acids

169.6

Weight (kDa)

8.56

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 363 - 499 1.1e-07 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 376 - 464 4e-10 Retrotransposon gag protein
gag-asp_proteas PF13975 638 - 725 3.4e-08 gag-polyprotein putative aspartyl protease
RVP_2 PF08284 643 - 726 7.3e-09 Retroviral aspartyl protease
RVT_1 PF00078 878 - 1038 2.3e-40 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 1100 - 1194 5.1e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 1125 - 1224 6.3e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 1311 - 1365 3.1e-15 Integrase zinc binding domain
rve PF00665 1386 - 1480 9.7e-07 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 111
Acc36I ACCTGC 3 cut(s) 1597, 2068, 3701
AccB1I GGYRCC 3 cut(s) 217, 1528, 1783
AccB7I CCANNNNNTGG 1 cut(s) 1828
AccI GTMKAC 2 cut(s) 209, 2667
AciI CCGC 3 cut(s) 696, 1607, 2656
AclWI GGATC 6 cut(s) 265, 589, 1046, 3131, 3917, 4352
AcoI YGGCCR 4 cut(s) 1452, 2040, 4031, 4450
AcsI RAATTY 9 cut(s) 664, 706, 733, 1184, 1313, 2197, 2344, 3386, 4021
AcuI CTGAAG 6 cut(s) 110, 291, 1556, 2234, 3353, 3393
AdeI CACNNNGTG 1 cut(s) 1933
AfiI CCNNNNNNNGG 9 cut(s) 275, 382, 1161, 1613, 1828, 2138, 2301, 4521, 4522
AflII CTTAAG 3 cut(s) 3185, 3278, 4369
AflIII ACRYGT 1 cut(s) 3106
AgeI ACCGGT 2 cut(s) 229, 4129
AhdI GACNNNNNGTC 1 cut(s) 2779
AhlI ACTAGT 3 cut(s) 344, 392, 431
AjnI CCWGG 8 cut(s) 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
AleI CACNNNNGTG 2 cut(s) 3356, 4150
AloI GAACNNNNNNTCC 2 cut(s) 1151, 1183
Alw21I GWGCWC 5 cut(s) 102, 239, 338, 1417, 3012
Alw26I GTCTC 9 cut(s) 68, 1341, 2029, 2054, 2178, 2509, 2923, 3812, 4269
AlwI GGATC 6 cut(s) 265, 589, 1046, 3131, 3917, 4352
AlwNI CAGNNNCTG 2 cut(s) 1360, 3716
ApoI RAATTY 9 cut(s) 664, 706, 733, 1184, 1313, 2197, 2344, 3386, 4021
AsiGI ACCGGT 2 cut(s) 229, 4129
Asp700I GAANNNNTTC 2 cut(s) 164, 3736
AspLEI GCGC 2 cut(s) 112, 2077
AspS9I GGNCC 3 cut(s) 1111, 3300, 3713
AsuC2I CCSGG 1 cut(s) 354
AsuNHI GCTAGC 2 cut(s) 2273, 3361
AvaII GGWCC 3 cut(s) 1111, 3300, 3713
AxyI CCTNAGG 1 cut(s) 1107
BaeI ACNNNNGTAYC 4 cut(s) 956, 989, 2402, 2435
BalI TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
BanI GGYRCC 3 cut(s) 217, 1528, 1783
BanII GRGCYC 7 cut(s) 239, 338, 1417, 2164, 2364, 2680, 4122
BarI GAAGNNNNNNTAC 6 cut(s) 2690, 2722, 3854, 3886, 4235, 4267
Bbv12I GWGCWC 5 cut(s) 102, 239, 338, 1417, 3012
BccI CCATC 8 cut(s) 175, 1738, 1804, 1983, 2065, 2639, 2713, 4442
BceAI ACGGC 2 cut(s) 915, 1818
BciT130I CCWGG 8 cut(s) 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
BciVI GTATCC 1 cut(s) 2430
BclI TGATCA 2 cut(s) 3111, 3750
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 9 cut(s) 68, 1341, 2029, 2054, 2178, 2509, 2923, 3812, 4269
BcuI ACTAGT 3 cut(s) 344, 392, 431
BfmI CTRYAG 5 cut(s) 210, 1842, 2671, 3132, 3309
BfrI CTTAAG 3 cut(s) 3185, 3278, 4369
BfuAI ACCTGC 3 cut(s) 1597, 2068, 3701
BfuI GTATCC 1 cut(s) 2430
BglI GCCNNNNNGGC 2 cut(s) 3128, 4117
BglII AGATCT 2 cut(s) 1458, 4421
BmcAI AGTACT 3 cut(s) 1036, 3608, 4487
Bme1390I CCNGG 9 cut(s) 354, 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
Bme18I GGWCC 3 cut(s) 1111, 3300, 3713
BmeRI GACNNNNNGTC 1 cut(s) 2779
BmgT120I GGNCC 3 cut(s) 1111, 3300, 3713
BmiI GGNNCC 5 cut(s) 219, 316, 1530, 1785, 3302
BmrFI CCNGG 9 cut(s) 354, 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
BmrI ACTGGG 2 cut(s) 990, 2615
BmsI GCATC 5 cut(s) 2793, 2859, 3165, 3394, 3577
BmtI GCTAGC 2 cut(s) 2277, 3365
BmuI ACTGGG 2 cut(s) 990, 2615
BplI GAGNNNNNCTC 2 cut(s) 1139, 1171
BpmI CTGGAG 1 cut(s) 3906
Bpu10I CCTNAGC 1 cut(s) 337
BpuEI CTTGAG 4 cut(s) 220, 2797, 2959, 4510
BpuMI CCSGG 1 cut(s) 354
Bsa29I ATCGAT 1 cut(s) 2736
BsaBI GATNNNNATC 1 cut(s) 4356
BsaI GGTCTC 3 cut(s) 1341, 2509, 2923
BsaWI WCCGGW 4 cut(s) 229, 1054, 3139, 4129
Bsc4I CCNNNNNNNGG 9 cut(s) 275, 382, 1161, 1613, 1828, 2138, 2301, 4521, 4522
Bse118I RCCGGY 3 cut(s) 229, 1786, 4129
Bse21I CCTNAGG 1 cut(s) 1107
Bse3DI GCAATG 1 cut(s) 1133
Bse8I GATNNNNATC 1 cut(s) 4356
BseBI CCWGG 8 cut(s) 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
BseCI ATCGAT 1 cut(s) 2736
BseJI GATNNNNATC 1 cut(s) 4356
BseLI CCNNNNNNNGG 9 cut(s) 275, 382, 1161, 1613, 1828, 2138, 2301, 4521, 4522
BseMI GCAATG 1 cut(s) 1133
BseRI GAGGAG 3 cut(s) 1059, 1062, 3968
BseYI CCCAGC 2 cut(s) 3986, 4521
BsgI GTGCAG 3 cut(s) 2334, 3794, 4061
BshNI GGYRCC 3 cut(s) 217, 1528, 1783
BshTI ACCGGT 2 cut(s) 229, 4129
BshVI ATCGAT 1 cut(s) 2736
BsiHKAI GWGCWC 5 cut(s) 102, 239, 338, 1417, 3012
BsiSI CCGG 7 cut(s) 230, 353, 1055, 1787, 3123, 3140, 4130
BslFI GGGAC 5 cut(s) 355, 553, 3094, 3117, 3421
BslI CCNNNNNNNGG 9 cut(s) 275, 382, 1161, 1613, 1828, 2138, 2301, 4521, 4522
BsmAI GTCTC 9 cut(s) 68, 1341, 2029, 2054, 2178, 2509, 2923, 3812, 4269
BsmFI GGGAC 5 cut(s) 355, 553, 3094, 3117, 3421
BsmI GAATGC 7 cut(s) 166, 622, 1091, 1843, 3043, 4335, 4388
Bso31I GGTCTC 3 cut(s) 1341, 2509, 2923
Bsp1286I GDGCHC 9 cut(s) 102, 239, 338, 1417, 2164, 2364, 2680, 3012, 4122
Bsp1407I TGTACA 2 cut(s) 2979, 3538
Bsp19I CCATGG 3 cut(s) 882, 3814, 4167
BspACI CCGC 3 cut(s) 696, 1607, 2656
BspDI ATCGAT 1 cut(s) 2736
BspHI TCATGA 2 cut(s) 2356, 3462
BspLI GGNNCC 5 cut(s) 219, 316, 1530, 1785, 3302
BspMAI CTGCAG 1 cut(s) 3313
BspMI ACCTGC 3 cut(s) 1597, 2068, 3701
BspOI GCTAGC 2 cut(s) 2277, 3365
BspPI GGATC 6 cut(s) 265, 589, 1046, 3131, 3917, 4352
BspT107I GGYRCC 3 cut(s) 217, 1528, 1783
BspTI CTTAAG 3 cut(s) 3185, 3278, 4369
BspTNI GGTCTC 3 cut(s) 1341, 2509, 2923
BsrDI GCAATG 1 cut(s) 1133
BsrFI RCCGGY 3 cut(s) 229, 1786, 4129
BsrGI TGTACA 2 cut(s) 2979, 3538
BssAI RCCGGY 3 cut(s) 229, 1786, 4129
BssT1I CCWWGG 6 cut(s) 489, 882, 1998, 3814, 4167, 4476
Bst2UI CCWGG 8 cut(s) 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
Bst6I CTCTTC 4 cut(s) 1240, 1354, 1461, 1761
BstAFI CTTAAG 3 cut(s) 3185, 3278, 4369
BstAPI GCANNNNNTGC 1 cut(s) 4330
BstAUI TGTACA 2 cut(s) 2979, 3538
BstC8I GCNNGC 8 cut(s) 36, 334, 1452, 2271, 2275, 3363, 3744, 4386
BstDSI CCRYGG 4 cut(s) 882, 897, 3814, 4167
BstENI CCTNNNNNAGG 2 cut(s) 1159, 2136
BstHHI GCGC 2 cut(s) 112, 2077
BstMAI GTCTC 9 cut(s) 68, 1341, 2029, 2054, 2178, 2509, 2923, 3812, 4269
BstNI CCWGG 8 cut(s) 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
BstNSI RCATGY 7 cut(s) 1446, 1871, 1940, 3110, 3277, 3807, 4097
BstSCI CCNGG 9 cut(s) 352, 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
BstSFI CTRYAG 5 cut(s) 210, 1842, 2671, 3132, 3309
BstX2I RGATCY 7 cut(s) 257, 1051, 1458, 3136, 3909, 4357, 4421
BstXI CCANNNNNNTGG 2 cut(s) 578, 4152
BstYI RGATCY 7 cut(s) 257, 1051, 1458, 3136, 3909, 4357, 4421
Bsu15I ATCGAT 1 cut(s) 2736
Bsu36I CCTNAGG 1 cut(s) 1107
BsuI GTATCC 1 cut(s) 2430
BsuTUI ATCGAT 1 cut(s) 2736
BtgI CCRYGG 4 cut(s) 882, 897, 3814, 4167
BtsI GCAGTG 2 cut(s) 102, 3531
BveI ACCTGC 3 cut(s) 1597, 2068, 3701
Cac8I GCNNGC 8 cut(s) 36, 334, 1452, 2271, 2275, 3363, 3744, 4386
CaiI CAGNNNCTG 2 cut(s) 1360, 3716
CciI TCATGA 2 cut(s) 2356, 3462
CfoI GCGC 2 cut(s) 112, 2077
Cfr10I RCCGGY 3 cut(s) 229, 1786, 4129
Cfr13I GGNCC 3 cut(s) 1111, 3300, 3713
ClaI ATCGAT 1 cut(s) 2736
CsiI ACCWGGT 1 cut(s) 1929
CspAI ACCGGT 2 cut(s) 229, 4129
CspCI CAANNNNNGTGG 2 cut(s) 3629, 3664
DraIII CACNNNGTG 1 cut(s) 1933
DriI GACNNNNNGTC 1 cut(s) 2779
EaeI YGGCCR 4 cut(s) 1452, 2040, 4031, 4450
Eam1104I CTCTTC 4 cut(s) 1240, 1354, 1461, 1761
Eam1105I GACNNNNNGTC 1 cut(s) 2779
EarI CTCTTC 4 cut(s) 1240, 1354, 1461, 1761
Ecl136II GAGCTC 3 cut(s) 237, 336, 1415
Eco130I CCWWGG 6 cut(s) 489, 882, 1998, 3814, 4167, 4476
Eco147I AGGCCT 5 cut(s) 1154, 1629, 1711, 3553, 3736
Eco24I GRGCYC 7 cut(s) 239, 338, 1417, 2164, 2364, 2680, 4122
Eco31I GGTCTC 3 cut(s) 1341, 2509, 2923
Eco47I GGWCC 3 cut(s) 1111, 3300, 3713
Eco53kI GAGCTC 3 cut(s) 237, 336, 1415
Eco57I CTGAAG 6 cut(s) 110, 291, 1556, 2234, 3353, 3393
Eco81I CCTNAGG 1 cut(s) 1107
EcoICRI GAGCTC 3 cut(s) 237, 336, 1415
EcoNI CCTNNNNNAGG 2 cut(s) 1159, 2136
EcoO109I RGGNCCY 1 cut(s) 3713
EcoRI GAATTC 3 cut(s) 1184, 1313, 2344
EcoRII CCWGG 8 cut(s) 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
EcoT14I CCWWGG 6 cut(s) 489, 882, 1998, 3814, 4167, 4476
EcoT22I ATGCAT 1 cut(s) 2808
EcoT38I GRGCYC 7 cut(s) 239, 338, 1417, 2164, 2364, 2680, 4122
ErhI CCWWGG 6 cut(s) 489, 882, 1998, 3814, 4167, 4476
FalI AAGNNNNNCTT 6 cut(s) 2759, 2791, 3721, 3753, 4408, 4440
FaqI GGGAC 5 cut(s) 355, 553, 3094, 3117, 3421
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 2 cut(s) 3111, 3750
FblI GTMKAC 2 cut(s) 209, 2667
FriOI GRGCYC 7 cut(s) 239, 338, 1417, 2164, 2364, 2680, 4122
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 2 cut(s) 111, 2076
GsaI CCCAGC 2 cut(s) 3990, 4525
GsuI CTGGAG 1 cut(s) 3906
HapII CCGG 7 cut(s) 230, 353, 1055, 1787, 3123, 3140, 4130
HhaI GCGC 2 cut(s) 112, 2077
Hin6I GCGC 2 cut(s) 110, 2075
HinP1I GCGC 2 cut(s) 110, 2075
HincII GTYRAC 2 cut(s) 4085, 4339
HindII GTYRAC 2 cut(s) 4085, 4339
HindIII AAGCTT 2 cut(s) 1737, 4182
HpaI GTTAAC 1 cut(s) 4339
HpaII CCGG 7 cut(s) 230, 353, 1055, 1787, 3123, 3140, 4130
HspAI GCGC 2 cut(s) 110, 2075
Ksp22I TGATCA 2 cut(s) 3111, 3750
KspAI GTTAAC 1 cut(s) 4339
LmnI GCTCC 9 cut(s) 97, 121, 278, 1279, 1955, 2317, 3007, 3425, 3955
LweI GCATC 5 cut(s) 2793, 2859, 3165, 3394, 3577
MabI ACCWGGT 1 cut(s) 1929
MboII GAAGA 9 cut(s) 1257, 1322, 1341, 1363, 1478, 1778, 3338, 3874, 4416
MfeI CAATTG 1 cut(s) 3453
MflI RGATCY 7 cut(s) 257, 1051, 1458, 3136, 3909, 4357, 4421
MhlI GDGCHC 9 cut(s) 102, 239, 338, 1417, 2164, 2364, 2680, 3012, 4122
MlsI TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
MluNI TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
MlyI GAGTC 6 cut(s) 161, 215, 299, 540, 1501, 2874
MmeI TCCRAC 6 cut(s) 495, 781, 808, 2003, 2119, 2556
Mox20I TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
Mph1103I ATGCAT 1 cut(s) 2808
MroXI GAANNNNTTC 2 cut(s) 164, 3736
MscI TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
MslI CAYNNNNRTG 6 cut(s) 1404, 2975, 3272, 3356, 3833, 4150
Msp20I TGGCCA 4 cut(s) 1454, 2042, 4033, 4452
MspA1I CMGCKG 3 cut(s) 3536, 4280, 4521
MspCI CTTAAG 3 cut(s) 3185, 3278, 4369
MspI CCGG 7 cut(s) 230, 353, 1055, 1787, 3123, 3140, 4130
MspR9I CCNGG 9 cut(s) 354, 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
MunI CAATTG 1 cut(s) 3453
Mva1269I GAATGC 7 cut(s) 166, 622, 1091, 1843, 3043, 4335, 4388
MvaI CCWGG 8 cut(s) 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
NciI CCSGG 1 cut(s) 354
NcoI CCATGG 3 cut(s) 882, 3814, 4167
NdeI CATATG 1 cut(s) 988
NheI GCTAGC 2 cut(s) 2273, 3361
NlaIV GGNNCC 5 cut(s) 219, 316, 1530, 1785, 3302
NmuCI GTSAC 5 cut(s) 484, 493, 1919, 2689, 4012
NsbI TGCGCA 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 2808
NspI RCATGY 7 cut(s) 1446, 1871, 1940, 3110, 3277, 3807, 4097
OliI CACNNNNGTG 2 cut(s) 3356, 4150
PagI TCATGA 2 cut(s) 2356, 3462
PceI AGGCCT 5 cut(s) 1154, 1629, 1711, 3553, 3736
PciI ACATGT 1 cut(s) 3106
PctI GAATGC 7 cut(s) 166, 622, 1091, 1843, 3043, 4335, 4388
PdmI GAANNNNTTC 2 cut(s) 164, 3736
PfeI GAWTC 9 cut(s) 455, 982, 1357, 1949, 2008, 2335, 3291, 3580, 3719
PflMI CCANNNNNTGG 1 cut(s) 1828
PinAI ACCGGT 2 cut(s) 229, 4129
PleI GAGTC 6 cut(s) 160, 214, 299, 539, 1501, 2874
PpsI GAGTC 6 cut(s) 160, 214, 299, 539, 1501, 2874
PpuMI RGGWCCY 1 cut(s) 3713
PscI ACATGT 1 cut(s) 3106
Psp124BI GAGCTC 3 cut(s) 239, 338, 1417
Psp5II RGGWCCY 1 cut(s) 3713
Psp6I CCWGG 8 cut(s) 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
PspFI CCCAGC 2 cut(s) 3986, 4521
PspGI CCWGG 8 cut(s) 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
PspN4I GGNNCC 5 cut(s) 219, 316, 1530, 1785, 3302
PspPI GGNCC 3 cut(s) 1111, 3300, 3713
PspPPI RGGWCCY 1 cut(s) 3713
PstI CTGCAG 1 cut(s) 3313
PstNI CAGNNNCTG 2 cut(s) 1360, 3716
PsuI RGATCY 7 cut(s) 257, 1051, 1458, 3136, 3909, 4357, 4421
PvuII CAGCTG 3 cut(s) 3536, 4280, 4521
RseI CAYNNNNRTG 6 cut(s) 1404, 2975, 3272, 3356, 3833, 4150
SacI GAGCTC 3 cut(s) 239, 338, 1417
Sau96I GGNCC 3 cut(s) 1111, 3300, 3713
ScaI AGTACT 3 cut(s) 1036, 3608, 4487
SchI GAGTC 6 cut(s) 161, 215, 299, 540, 1501, 2874
ScrFI CCNGG 9 cut(s) 354, 1713, 1823, 1931, 2975, 3305, 3563, 4035, 4116
SduI GDGCHC 9 cut(s) 102, 239, 338, 1417, 2164, 2364, 2680, 3012, 4122
SexAI ACCWGGT 1 cut(s) 1929
SfaNI GCATC 5 cut(s) 2793, 2859, 3165, 3394, 3577
SfcI CTRYAG 5 cut(s) 210, 1842, 2671, 3132, 3309
SinI GGWCC 3 cut(s) 1111, 3300, 3713
SmiMI CAYNNNNRTG 6 cut(s) 1404, 2975, 3272, 3356, 3833, 4150
SmlI CTYRAG 7 cut(s) 199, 2776, 2938, 3185, 3278, 4369, 4489
SmoI CTYRAG 7 cut(s) 199, 2776, 2938, 3185, 3278, 4369, 4489
SpeI ACTAGT 3 cut(s) 344, 392, 431
SseBI AGGCCT 5 cut(s) 1154, 1629, 1711, 3553, 3736
SsiI CCGC 3 cut(s) 696, 1607, 2656
SspI AATATT 1 cut(s) 2243
SstI GAGCTC 3 cut(s) 239, 338, 1417
StuI AGGCCT 5 cut(s) 1154, 1629, 1711, 3553, 3736
StyD4I CCNGG 9 cut(s) 352, 1711, 1821, 1929, 2973, 3303, 3561, 4033, 4114
StyI CCWWGG 6 cut(s) 489, 882, 1998, 3814, 4167, 4476
TaqI TCGA 4 cut(s) 193, 1243, 2481, 2736
TaqII GACCGA 1 cut(s) 1128
TatI WGTACW 5 cut(s) 1034, 2979, 3538, 3606, 4485
TfiI GAWTC 9 cut(s) 455, 982, 1357, 1949, 2008, 2335, 3291, 3580, 3719
TseFI GTSAC 5 cut(s) 484, 493, 1919, 2689, 4012
Tsp45I GTSAC 5 cut(s) 484, 493, 1919, 2689, 4012
TspGWI ACGGA 1 cut(s) 158
Van91I CCANNNNNTGG 1 cut(s) 1828
Vha464I CTTAAG 3 cut(s) 3185, 3278, 4369
VpaK11BI GGWCC 3 cut(s) 1111, 3300, 3713
XagI CCTNNNNNAGG 2 cut(s) 1159, 2136
XapI RAATTY 9 cut(s) 664, 706, 733, 1184, 1313, 2197, 2344, 3386, 4021
XceI RCATGY 7 cut(s) 1446, 1871, 1940, 3110, 3277, 3807, 4097
XcmI CCANNNNNNNNNTGG 3 cut(s) 325, 1522, 3930
XmiI GTMKAC 2 cut(s) 209, 2667
XmnI GAANNNNTTC 2 cut(s) 164, 3736
ZrmI AGTACT 3 cut(s) 1036, 3608, 4487
Zsp2I ATGCAT 1 cut(s) 2808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.