Rw5G005440

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000756
Physical Location & Seq
Forward (+)
5703297 .. 5711061
7765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005440.1

Sequence Viewer

Length: 3054 bp
ATGCAACTTAAGGGGTTCTGCAACAAAAAGAAGGTTCATGTTTTGATTGACTCAGGGGCCTCACATAATTTCGTCCACCCTATACTACTTAAAAATGTGAGGGCTGTTACGAGGAACATTACACCAATGAGAGTAAGATTAGCTAGTGGTGAGATCTTACAAACCTCCAGAGTGGCTGAATTGGAATTGAACCTTCAGGGGTACTCGTTCACAGCCAGCTATTATGTATTGCCTATTTCAGGCAGTGAGATCATTTTGGGAGGAAGTTGGCTTAAAACACTCGGGGATATTACTTGGAACTTTGAATTTTTGACCATGAACTTTATGTGTGGTGAAACTGTTCATTTTTTGCAGGGTGAATCTACCTCTGAAGCCACAATTGTTAACTGCAAGTCCATGACAAGGTTGTTGAGGAAAGAAAGGGAGGCTGTATTAGTTCAAGTCCAAGGCCTTACAGCTCTACCTTCTGAGTTAACCACTTCCCCGGATATTCAGAGGTTGATCGGAAAGTACTCAGATGTCTTTGAAGCACCGAAACACCTACCTCCCACTCGAGAGCAGGACCACAGAATTGAACTCTTACCTAACACTGTTGCCGTCAACGTGAGACCATACAGATATCCCCATTTCCAAAAGGGGGAGATTGAAAGAATTATCCAAGACCCTTTCTCTTCACCTGTTCTTCTGGTAAAAAAGAAAGATGGGACATGGAGGCTTTGTGTCGATTATCGAGCTTTGAATGCTGCCACTATCAAGGACAAGTATCCCATTCCTGTAGTGGACGAGCTCATCGATGAAGTACATGGAGCAGTAATCTTCAGTAAATTAGATCTAAGGTCGGGTTACCACCAGATAAGAATGCGTGAGAGGGATATCAACAAAACCGCTTTTCGAACACATTCTGGCCATTATGAATTCTTGGTGATGCCTTTCGGCTTGACGAATGCTCCTGCAACATTTCAATCGGTCATGAATGATGTATTGAGGGATTTCTTAAGGAAATTTGTACTAGTGTTCTTTGATGACATTTTGATCTGCAGTACTTCTGTGGCTGAGCACCTAAGCCACTTAGAGCAAGTGTTCCAGCGGCTGCAGCAACATTCACTGAAAATCAAAGCCAGCAAGTGTAGCTTTGGCGTCCCACAGGTGGAGTATTTAGGTCACATTATCAAGGCTGAAGGTGTGGTTGTGGACCCGGCAAAAATAGAGTGTATCAAGCAATGGCCAAAACCAAAAACTCTCAAGGAACTACGTGGGTTTCTCGGATTAGCTGGCTACTACAAAAAGTATGTCAAAGGCTTCGGAGTCATAGCTAAGCCTCTAACTGACATGTTGAAAAAAGACGCTTTTCATTGGAGTGAGAAAGCAATGGTAGCCTTTGAAGAGCTTAAAACGGCTCTTATCTCTACTCCTGTACTAGCCATACCGGATTTCTCCAAAGAGTTTGTGATCGAATGCGACGCTTCTGATTTAGGCATCGGTGCCGTGCTTTCCCAAGAAGGCCATCCCATTGCATTCATGAGTAAAGCATTGTCTCAAAGACATTTGGCACATTCCGTTTATGACAAAGAAATGCTGGCAGTGGTAGCGGCTGTACAACATTGGAGACCATACCTTTTGGGTCGCCATTTCAAGATTTTCACTGACCACAAAACTATAGAACATTTCTTGAGCCACAATGCTGCACCGGATGCGTTGTCTCGTTCGCCCTACCTCTGTGGCATCACTGGGATTTCTACACCAGTGCATACTTATGTTTCTGAAATTCAGCAGGCTTGTTTAAAGGATAAGGAAGCTCACCAGATCATACAGGCACTACAACTTAATCCTGCAGGTAAGAAACATTATTCCTTACACAACTCTCAGTTGTTTTACAAAAACAAAATCTTTAAATTTGTGGCTGAATGTGAGGTGTGCCAAAAGAACCATTATGAGACCATCAAACCACCAGGTTTATTGCAGCCATTGGCTATTCCGGACAGGGCTTGGAGCACTATCTCAATGGATTTTATTGATGCTTTACCGAAATCTGAAGTGGATCACCAACTGAGGGACAGAGATATGTTATTAGCTGTGATAAAGCGAAACCTTGTGCAAGCTCAGACAAGAATGAAAAGCTTCCATGACAAAAAACACTCTGACCGGACTTTTGAGGTGGGTGACATGGTCTTTCTCAAACTCCAACCCTACAGACAACAAACTGCGCATCAAGGTAAAATCGGTTCCGTAGCTTATAGACTTGAGCTGCCGGCTGGTGCTAGAATACACAATGTATTCCATGTTTCACTTCTAAAAAAGAGAGTTGGGGAGTCTGTCCAAGTGAGCAACCACTTACCACCCATCATGGACCCAGCCAACCCCAAGTGGGAACCATCTACTGTGCTCCAAAGAAAAATGGTGAAACGCAACGGTGCTGCAGCTACTCAGTGGCTCATACATTGGCTGGGTACAGCTCCTGAGGAGGCCACGTGGGAATACGCTGATGAGATCCTTCTCCGATTTCCCAGGTTGCTACCCTATCAGATTCAGTCTATTCCTTTAGAAAATCTCAATTTATTTGATGGTCTGTGGAAATTCTGTCTTCAGATTCTGGACACAAATCCCCAATTATTTTTCCCTCTCCAACAGCAAAGGTTGATAGAACTAATTAGGAATGGAAAGGTAAAAGAGGCTCTTGAATTTGCTCAGAAAGAGCTAGCACCAAGAGGAGAAGAAAATACGATGTCCAGTCAGCTAACCCCTACCTTCAGGCAAAAATGTGACAATAAAGGGCAACTCTGCTTTTCGCCTCATGATGAACGTGCCGATTACTACAATGGCGAGTCTGATGATGACAAACTGGATCCAAATAGGTCCAGAAGAGCTCGAGCCCACACCATCATGAAGTTTTCATCAAGAGAGTCTGCAAATTACACCCGCAAACATCACGCCACGCACGTGGACCCAAGCCACGTTCATGCACTTGGGGCTTACAAGAATGGGTGTGGTCTTTATTCCATTTCGAATTCAACTTTATTTCATTTTGAAATCAGCTTTATTCCATTTCAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1017

Amino Acids

115.96

Weight (kDa)

9.02

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag-asp_proteas PF13975 7 - 92 4.4e-06 gag-polyprotein putative aspartyl protease
RVP_2 PF08284 8 - 92 2.1e-06 Retroviral aspartyl protease
RVT_1 PF00078 230 - 390 6.9e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 452 - 546 5e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 477 - 562 2.6e-28 RNase H-like domain found in reverse transcriptase
SH3_Tf2-1 PF24626 739 - 766 1.5e-08 Tf2-1-like, SH3 domain
CTLH PF10607 862 - 907 6.9e-09 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2205
Acc36I ACCTGC 1 cut(s) 1823
AccB1I GGYRCC 1 cut(s) 1481
AccIII TCCGGA 1 cut(s) 1975
AciI CCGC 4 cut(s) 885, 1087, 1589, 2917
AclWI GGATC 4 cut(s) 2046, 2482, 2837, 2850
AcoI YGGCCR 2 cut(s) 904, 1223
AcsI RAATTY 8 cut(s) 305, 914, 1001, 1764, 1892, 2573, 2678, 3004
AcuI CTGAAG 7 cut(s) 179, 390, 802, 1197, 2052, 2567, 2731
AcvI CACGTG 2 cut(s) 2469, 2938
AcyI GRCGYC 1 cut(s) 1137
AfaI GTAC 8 cut(s) 203, 512, 801, 1008, 1042, 1416, 1596, 2449
AfiI CCNNNNNNNGG 6 cut(s) 239, 402, 637, 1147, 2050, 2365
AflII CTTAAG 2 cut(s) 8, 994
AflIII ACRYGT 1 cut(s) 1329
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 2 cut(s) 1948, 2504
AleI CACNNNNGTG 1 cut(s) 2936
Alw21I GWGCWC 5 cut(s) 789, 1059, 1994, 2385, 2866
Alw26I GTCTC 5 cut(s) 601, 1539, 1600, 1704, 1928
AlwI GGATC 4 cut(s) 2046, 2482, 2837, 2850
AlwNI CAGNNNCTG 3 cut(s) 1090, 2456, 2590
Ama87I CYCGRG 3 cut(s) 281, 552, 2865
Aor13HI TCCGGA 1 cut(s) 1975
AoxI GGCC 6 cut(s) 57, 448, 904, 1223, 1501, 2463
ApeKI GCWGC 8 cut(s) 743, 1090, 1093, 1682, 1960, 2245, 2414, 2417
ApoI RAATTY 8 cut(s) 305, 914, 1001, 1764, 1892, 2573, 2678, 3004
ArsI GACNNNNNNTTYG 4 cut(s) 1275, 1307, 2132, 2164
Asp700I GAANNNNTTC 3 cut(s) 339, 898, 2117
AspLEI GCGC 1 cut(s) 2206
AspS9I GGNCC 6 cut(s) 57, 562, 1192, 2347, 2853, 2941
AsuC2I CCSGG 2 cut(s) 485, 1196
AsuHPI GGTGA 9 cut(s) 161, 344, 368, 666, 934, 1790, 2033, 2171, 2410
AsuII TTCGAA 2 cut(s) 892, 3002
AsuNHI GCTAGC 1 cut(s) 2695
AvaI CYCGRG 3 cut(s) 281, 552, 2865
AvaII GGWCC 5 cut(s) 562, 1192, 2347, 2853, 2941
AxyI CCTNAGG 1 cut(s) 2457
BalI TGGCCA 2 cut(s) 906, 1225
BamHI GGATCC 1 cut(s) 2842
BanI GGYRCC 1 cut(s) 1481
BanII GRGCYC 3 cut(s) 789, 2866, 2872
BbrPI CACGTG 2 cut(s) 2469, 2938
BbsI GAAGAC 1 cut(s) 2573
Bbv12I GWGCWC 5 cut(s) 789, 1059, 1994, 2385, 2866
BbvI GCAGC 8 cut(s) 730, 1077, 1105, 1669, 1972, 2232, 2401, 2429
BccI CCATC 7 cut(s) 695, 1512, 1946, 2348, 2380, 2555, 2885
BceAI ACGGC 3 cut(s) 581, 1410, 1469
BciT130I CCWGG 2 cut(s) 1950, 2506
BciVI GTATCC 1 cut(s) 774
BcnI CCSGG 2 cut(s) 485, 1196
BcoDI GTCTC 5 cut(s) 601, 1539, 1600, 1704, 1928
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 5 cut(s) 144, 1010, 1418, 2259, 2696
BfmI CTRYAG 7 cut(s) 774, 1036, 1091, 1656, 1830, 2188, 2415
BfrI CTTAAG 2 cut(s) 8, 994
BfuAI ACCTGC 1 cut(s) 1823
BfuI GTATCC 1 cut(s) 774
BglII AGATCT 2 cut(s) 153, 829
BlpI GCTNAGC 2 cut(s) 1053, 1314
BmcAI AGTACT 2 cut(s) 512, 1042
Bme1390I CCNGG 4 cut(s) 485, 1196, 1950, 2506
Bme18I GGWCC 5 cut(s) 562, 1192, 2347, 2853, 2941
BmeT110I CYCGRG 3 cut(s) 281, 552, 2865
BmgT120I GGNCC 6 cut(s) 57, 562, 1192, 2347, 2853, 2941
BmiI GGNNCC 8 cut(s) 58, 1194, 1483, 2224, 2349, 2370, 2844, 2943
BmrFI CCNGG 4 cut(s) 485, 1196, 1950, 2506
BmrI ACTGGG 1 cut(s) 1737
BmsI GCATC 6 cut(s) 915, 1485, 1681, 1731, 2005, 2215
BmtI GCTAGC 1 cut(s) 2699
BmuI ACTGGG 1 cut(s) 1737
BpiI GAAGAC 1 cut(s) 2573
BpmI CTGGAG 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 1061
Bpu1102I GCTNAGC 2 cut(s) 1053, 1314
Bpu14I TTCGAA 2 cut(s) 892, 3002
BpuEI CTTGAG 3 cut(s) 1226, 1690, 2261
BpuMI CCSGG 2 cut(s) 485, 1196
Bsa29I ATCGAT 1 cut(s) 792
BsaAI YACGTR 3 cut(s) 1253, 2469, 2938
BsaHI GRCGYC 1 cut(s) 1137
BsaI GGTCTC 3 cut(s) 601, 1600, 1928
BsaJI CCNNGG 3 cut(s) 445, 483, 2504
BsaWI WCCGGW 4 cut(s) 1426, 1687, 1975, 2142
BsaXI ACNNNNNCTCC 6 cut(s) 703, 733, 931, 961, 2453, 2483
Bsc4I CCNNNNNNNGG 6 cut(s) 239, 402, 637, 1147, 2050, 2365
Bse118I RCCGGY 1 cut(s) 2248
Bse1I ACTGG 4 cut(s) 1732, 1742, 2727, 2844
Bse21I CCTNAGG 1 cut(s) 2457
Bse3DI GCAATG 3 cut(s) 1226, 1374, 1509
BseAI TCCGGA 1 cut(s) 1975
BseBI CCWGG 2 cut(s) 1950, 2506
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 3 cut(s) 445, 483, 2504
BseGI GGATG 2 cut(s) 1504, 1696
BseLI CCNNNNNNNGG 6 cut(s) 239, 402, 637, 1147, 2050, 2365
BseMI GCAATG 3 cut(s) 1226, 1374, 1509
BseNI ACTGG 4 cut(s) 1732, 1742, 2727, 2844
BseRI GAGGAG 2 cut(s) 2474, 2721
BseXI GCAGC 8 cut(s) 730, 1077, 1105, 1669, 1972, 2232, 2401, 2429
BseYI CCCAGC 2 cut(s) 2350, 2443
BsgI GTGCAG 1 cut(s) 1668
BshFI GGCC 6 cut(s) 59, 450, 906, 1225, 1503, 2465
BshNI GGYRCC 1 cut(s) 1481
BshVI ATCGAT 1 cut(s) 792
BsiHKAI GWGCWC 5 cut(s) 789, 1059, 1994, 2385, 2866
BsiHKCI CYCGRG 3 cut(s) 281, 552, 2865
BsiSI CCGG 7 cut(s) 485, 1196, 1427, 1688, 1976, 2143, 2249
BslFI GGGAC 3 cut(s) 718, 1124, 2066
BslI CCNNNNNNNGG 6 cut(s) 239, 402, 637, 1147, 2050, 2365
BsmAI GTCTC 5 cut(s) 601, 1539, 1600, 1704, 1928
BsmFI GGGAC 3 cut(s) 718, 1124, 2066
BsmI GAATGC 5 cut(s) 745, 864, 949, 1460, 1514
BsnI GGCC 6 cut(s) 59, 450, 906, 1225, 1503, 2465
Bso31I GGTCTC 3 cut(s) 601, 1600, 1928
BsoBI CYCGRG 3 cut(s) 281, 552, 2865
Bsp119I TTCGAA 2 cut(s) 892, 3002
Bsp1286I GDGCHC 6 cut(s) 789, 1059, 1994, 2385, 2866, 2872
Bsp13I TCCGGA 1 cut(s) 1975
Bsp1407I TGTACA 1 cut(s) 1594
Bsp1720I GCTNAGC 2 cut(s) 1053, 1314
BspACI CCGC 4 cut(s) 885, 1087, 1589, 2917
BspANI GGCC 6 cut(s) 59, 450, 906, 1225, 1503, 2465
BspDI ATCGAT 1 cut(s) 792
BspEI TCCGGA 1 cut(s) 1975
BspHI TCATGA 4 cut(s) 969, 1518, 2791, 2880
BspLI GGNNCC 8 cut(s) 58, 1194, 1483, 2224, 2349, 2370, 2844, 2943
BspMAI CTGCAG 4 cut(s) 1040, 1095, 1834, 2419
BspMI ACCTGC 1 cut(s) 1823
BspOI GCTAGC 1 cut(s) 2699
BspPI GGATC 4 cut(s) 2046, 2482, 2837, 2850
BspQI GCTCTTC 2 cut(s) 1377, 2854
BspT104I TTCGAA 2 cut(s) 892, 3002
BspT107I GGYRCC 1 cut(s) 1481
BspTI CTTAAG 2 cut(s) 8, 994
BspTNI GGTCTC 3 cut(s) 601, 1600, 1928
BsrDI GCAATG 3 cut(s) 1226, 1374, 1509
BsrFI RCCGGY 1 cut(s) 2248
BsrGI TGTACA 1 cut(s) 1594
BsrI ACTGG 4 cut(s) 1732, 1742, 2727, 2844
BssAI RCCGGY 1 cut(s) 2248
BssECI CCNNGG 3 cut(s) 445, 483, 2504
BssNI GRCGYC 1 cut(s) 1137
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 1950, 2506
Bst4CI ACNGT 4 cut(s) 340, 592, 2380, 2411
Bst6I CTCTTC 3 cut(s) 676, 1377, 2854
BstACI GRCGYC 1 cut(s) 1137
BstAFI CTTAAG 2 cut(s) 8, 994
BstAPI GCANNNNNTGC 1 cut(s) 1691
BstAUI TGTACA 1 cut(s) 1594
BstBAI YACGTR 3 cut(s) 1253, 2469, 2938
BstBI TTCGAA 2 cut(s) 892, 3002
BstC8I GCNNGC 8 cut(s) 217, 1120, 1273, 1578, 1773, 2097, 2250, 2697
BstEII GGTNACC 1 cut(s) 842
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 1504, 1696
BstHHI GCGC 1 cut(s) 2206
BstMAI GTCTC 5 cut(s) 601, 1539, 1600, 1704, 1928
BstMWI GCNNNNNNNGC 7 cut(s) 740, 1093, 1128, 1373, 1586, 1691, 2966
BstNI CCWGG 2 cut(s) 1950, 2506
BstNSI RCATGY 1 cut(s) 1333
BstPI GGTNACC 1 cut(s) 842
BstSCI CCNGG 4 cut(s) 483, 1194, 1948, 2504
BstSFI CTRYAG 7 cut(s) 774, 1036, 1091, 1656, 1830, 2188, 2415
BstV1I GCAGC 8 cut(s) 730, 1077, 1105, 1669, 1972, 2232, 2401, 2429
BstV2I GAAGAC 1 cut(s) 2573
BstX2I RGATCY 4 cut(s) 153, 829, 2487, 2842
BstXI CCANNNNNNTGG 1 cut(s) 2938
BstYI RGATCY 4 cut(s) 153, 829, 2487, 2842
Bsu15I ATCGAT 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 2457
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 6 cut(s) 59, 450, 906, 1225, 1503, 2465
BsuTUI ATCGAT 1 cut(s) 792
BtsCI GGATG 2 cut(s) 1504, 1696
BtsI GCAGTG 2 cut(s) 250, 1587
BtsIMutI CAGTG 8 cut(s) 250, 588, 1103, 1587, 1641, 1725, 1749, 2432
BveI ACCTGC 1 cut(s) 1823
Cac8I GCNNGC 8 cut(s) 217, 1120, 1273, 1578, 1773, 2097, 2250, 2697
CaiI CAGNNNCTG 3 cut(s) 1090, 2456, 2590
CciI TCATGA 4 cut(s) 969, 1518, 2791, 2880
CfoI GCGC 1 cut(s) 2206
Cfr10I RCCGGY 1 cut(s) 2248
Cfr13I GGNCC 6 cut(s) 57, 562, 1192, 2347, 2853, 2941
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 3 cut(s) 1126, 1352, 1469
CsiI ACCWGGT 1 cut(s) 1948
Csp6I GTAC 8 cut(s) 202, 511, 800, 1007, 1041, 1415, 1595, 2448
CviQI GTAC 8 cut(s) 202, 511, 800, 1007, 1041, 1415, 1595, 2448
DraI TTTAAA 2 cut(s) 1782, 1891
EaeI YGGCCR 2 cut(s) 904, 1223
Eam1104I CTCTTC 3 cut(s) 676, 1377, 2854
EarI CTCTTC 3 cut(s) 676, 1377, 2854
Ecl136II GAGCTC 2 cut(s) 787, 2864
Eco130I CCWWGG 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 450
Eco24I GRGCYC 3 cut(s) 789, 2866, 2872
Eco31I GGTCTC 3 cut(s) 601, 1600, 1928
Eco32I GATATC 2 cut(s) 620, 874
Eco47I GGWCC 5 cut(s) 562, 1192, 2347, 2853, 2941
Eco53kI GAGCTC 2 cut(s) 787, 2864
Eco57I CTGAAG 7 cut(s) 179, 390, 802, 1197, 2052, 2567, 2731
Eco72I CACGTG 2 cut(s) 2469, 2938
Eco81I CCTNAGG 1 cut(s) 2457
Eco88I CYCGRG 3 cut(s) 281, 552, 2865
Eco91I GGTNACC 1 cut(s) 842
EcoICRI GAGCTC 2 cut(s) 787, 2864
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 57
EcoO65I GGTNACC 1 cut(s) 842
EcoRI GAATTC 2 cut(s) 914, 3004
EcoRII CCWGG 2 cut(s) 1948, 2504
EcoRV GATATC 2 cut(s) 620, 874
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 3 cut(s) 789, 2866, 2872
ErhI CCWWGG 1 cut(s) 445
FalI AAGNNNNNCTT 4 cut(s) 1115, 1147, 2658, 2690
FaqI GGGAC 3 cut(s) 718, 1124, 2066
FauI CCCGC 1 cut(s) 2924
FokI GGATG 2 cut(s) 1491, 1703
FriOI GRGCYC 3 cut(s) 789, 2866, 2872
FspBI CTAG 5 cut(s) 144, 1010, 1418, 2259, 2696
FspI TGCGCA 1 cut(s) 2205
GlaI GCGC 1 cut(s) 2205
GsaI CCCAGC 2 cut(s) 2354, 2447
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 6 cut(s) 59, 450, 906, 1225, 1503, 2465
HapII CCGG 7 cut(s) 485, 1196, 1427, 1688, 1976, 2143, 2249
HgaI GACGC 3 cut(s) 1126, 1352, 1469
HhaI GCGC 1 cut(s) 2206
Hin1I GRCGYC 1 cut(s) 1137
Hin6I GCGC 1 cut(s) 2204
HinP1I GCGC 1 cut(s) 2204
HincII GTYRAC 3 cut(s) 385, 474, 601
HindII GTYRAC 3 cut(s) 385, 474, 601
HindIII AAGCTT 1 cut(s) 2116
HinfI GANTC 8 cut(s) 50, 359, 1305, 2309, 2524, 2587, 2822, 2900
HpaI GTTAAC 2 cut(s) 385, 474
HpaII CCGG 7 cut(s) 485, 1196, 1427, 1688, 1976, 2143, 2249
HphI GGTGA 9 cut(s) 161, 344, 368, 666, 934, 1790, 2033, 2171, 2410
Hpy166II GTNNAC 8 cut(s) 76, 210, 385, 474, 601, 781, 1192, 2941
Hpy8I GTNNAC 8 cut(s) 76, 210, 385, 474, 601, 781, 1192, 2941
Hpy99I CGWCG 1 cut(s) 1463
HpyAV CCTTC 7 cut(s) 25, 203, 474, 1172, 1493, 2501, 2755
HpyCH4III ACNGT 4 cut(s) 340, 592, 2380, 2411
HpyCH4IV ACGT 6 cut(s) 603, 1252, 2468, 2800, 2937, 2952
HpyF10VI GCNNNNNNNGC 7 cut(s) 740, 1093, 1128, 1373, 1586, 1691, 2966
HpySE526I ACGT 6 cut(s) 603, 1252, 2468, 2800, 2937, 2952
Hsp92I GRCGYC 1 cut(s) 1137
HspAI GCGC 1 cut(s) 2204
Kpn2I TCCGGA 1 cut(s) 1975
KroI GCCGGC 1 cut(s) 2248
KroNI GCCGGC 1 cut(s) 2250
KspAI GTTAAC 2 cut(s) 385, 474
LguI GCTCTTC 2 cut(s) 1377, 2854
LmnI GCTCC 5 cut(s) 806, 952, 1989, 2388, 2458
Lsp1109I GCAGC 8 cut(s) 730, 1077, 1105, 1669, 1972, 2232, 2401, 2429
LweI GCATC 6 cut(s) 915, 1485, 1681, 1731, 2005, 2215
MabI ACCWGGT 1 cut(s) 1948
MaeI CTAG 5 cut(s) 144, 1010, 1418, 2259, 2696
MaeII ACGT 6 cut(s) 603, 1252, 2468, 2800, 2937, 2952
MaeIII GTNAC 5 cut(s) 106, 842, 1160, 2159, 2759
MboII GAAGA 7 cut(s) 663, 674, 808, 1394, 2573, 2723, 2871
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 4 cut(s) 153, 829, 2487, 2842
MhlI GDGCHC 6 cut(s) 789, 1059, 1994, 2385, 2866, 2872
MlsI TGGCCA 2 cut(s) 906, 1225
MluNI TGGCCA 2 cut(s) 906, 1225
MlyI GAGTC 5 cut(s) 44, 1314, 2318, 2831, 2909
MmeI TCCRAC 2 cut(s) 2206, 2647
Mox20I TGGCCA 2 cut(s) 906, 1225
MroI TCCGGA 1 cut(s) 1975
MroNI GCCGGC 1 cut(s) 2248
MroXI GAANNNNTTC 3 cut(s) 339, 898, 2117
MscI TGGCCA 2 cut(s) 906, 1225
MslI CAYNNNNRTG 5 cut(s) 1356, 1752, 2879, 2936, 2955
Msp20I TGGCCA 2 cut(s) 906, 1225
MspA1I CMGCKG 1 cut(s) 1087
MspCI CTTAAG 2 cut(s) 8, 994
MspI CCGG 7 cut(s) 485, 1196, 1427, 1688, 1976, 2143, 2249
MspR9I CCNGG 4 cut(s) 485, 1196, 1950, 2506
MunI CAATTG 1 cut(s) 378
Mva1269I GAATGC 5 cut(s) 745, 864, 949, 1460, 1514
MvaI CCWGG 2 cut(s) 1950, 2506
MwoI GCNNNNNNNGC 7 cut(s) 740, 1093, 1128, 1373, 1586, 1691, 2966
NaeI GCCGGC 1 cut(s) 2250
NciI CCSGG 2 cut(s) 485, 1196
NgoMIV GCCGGC 1 cut(s) 2248
NheI GCTAGC 1 cut(s) 2695
NlaIV GGNNCC 8 cut(s) 58, 1194, 1483, 2224, 2349, 2370, 2844, 2943
NmuCI GTSAC 3 cut(s) 1160, 2159, 2759
NsbI TGCGCA 1 cut(s) 2205
NspI RCATGY 1 cut(s) 1333
NspV TTCGAA 2 cut(s) 892, 3002
OliI CACNNNNGTG 1 cut(s) 2936
PaeR7I CTCGAG 2 cut(s) 552, 2865
PagI TCATGA 4 cut(s) 969, 1518, 2791, 2880
PceI AGGCCT 1 cut(s) 450
PciI ACATGT 1 cut(s) 1329
PciSI GCTCTTC 2 cut(s) 1377, 2854
PcsI WCGNNNNNNNCGW 2 cut(s) 789, 2934
PctI GAATGC 5 cut(s) 745, 864, 949, 1460, 1514
PdiI GCCGGC 1 cut(s) 2250
PdmI GAANNNNTTC 3 cut(s) 339, 898, 2117
PfeI GAWTC 3 cut(s) 359, 2524, 2587
PflFI GACNNNGTC 1 cut(s) 2165
PleI GAGTC 5 cut(s) 44, 1313, 2317, 2830, 2908
PmaCI CACGTG 2 cut(s) 2469, 2938
PmlI CACGTG 2 cut(s) 2469, 2938
PpsI GAGTC 5 cut(s) 44, 1313, 2317, 2830, 2908
Ppu21I YACGTR 3 cut(s) 1253, 2469, 2938
PscI ACATGT 1 cut(s) 1329
Psp124BI GAGCTC 2 cut(s) 789, 2866
Psp6I CCWGG 2 cut(s) 1948, 2504
PspCI CACGTG 2 cut(s) 2469, 2938
PspEI GGTNACC 1 cut(s) 842
PspFI CCCAGC 2 cut(s) 2350, 2443
PspGI CCWGG 2 cut(s) 1948, 2504
PspN4I GGNNCC 8 cut(s) 58, 1194, 1483, 2224, 2349, 2370, 2844, 2943
PspPI GGNCC 6 cut(s) 57, 562, 1192, 2347, 2853, 2941
PspXI VCTCGAGB 1 cut(s) 2865
PsrI GAACNNNNNNTAC 2 cut(s) 2206, 2238
PstI CTGCAG 4 cut(s) 1040, 1095, 1834, 2419
PstNI CAGNNNCTG 3 cut(s) 1090, 2456, 2590
PsuI RGATCY 4 cut(s) 153, 829, 2487, 2842
PsyI GACNNNGTC 1 cut(s) 2165
RsaI GTAC 8 cut(s) 203, 512, 801, 1008, 1042, 1416, 1596, 2449
RsaNI GTAC 8 cut(s) 202, 511, 800, 1007, 1041, 1415, 1595, 2448
RseI CAYNNNNRTG 5 cut(s) 1356, 1752, 2879, 2936, 2955
SacI GAGCTC 2 cut(s) 789, 2866
SapI GCTCTTC 2 cut(s) 1377, 2854
Sau96I GGNCC 6 cut(s) 57, 562, 1192, 2347, 2853, 2941
SbfI CCTGCAGG 1 cut(s) 1834
ScaI AGTACT 2 cut(s) 512, 1042
SchI GAGTC 5 cut(s) 44, 1314, 2318, 2831, 2909
ScrFI CCNGG 4 cut(s) 485, 1196, 1950, 2506
SdaI CCTGCAGG 1 cut(s) 1834
SduI GDGCHC 6 cut(s) 789, 1059, 1994, 2385, 2866, 2872
SexAI ACCWGGT 1 cut(s) 1948
SfaNI GCATC 6 cut(s) 915, 1485, 1681, 1731, 2005, 2215
SfcI CTRYAG 7 cut(s) 774, 1036, 1091, 1656, 1830, 2188, 2415
Sfr274I CTCGAG 2 cut(s) 552, 2865
SfuI TTCGAA 2 cut(s) 892, 3002
SinI GGWCC 5 cut(s) 562, 1192, 2347, 2853, 2941
SlaI CTCGAG 2 cut(s) 552, 2865
SmiMI CAYNNNNRTG 5 cut(s) 1356, 1752, 2879, 2936, 2955
SmlI CTYRAG 7 cut(s) 8, 552, 994, 1241, 1669, 2240, 2865
SmoI CTYRAG 7 cut(s) 8, 552, 994, 1241, 1669, 2240, 2865
SpeI ACTAGT 1 cut(s) 1009
Sse8387I CCTGCAGG 1 cut(s) 1834
SseBI AGGCCT 1 cut(s) 450
SsiI CCGC 4 cut(s) 885, 1087, 1589, 2917
SspMI CTAG 5 cut(s) 144, 1010, 1418, 2259, 2696
SstI GAGCTC 2 cut(s) 789, 2866
StuI AGGCCT 1 cut(s) 450
StyD4I CCNGG 4 cut(s) 483, 1194, 1948, 2504
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 4 cut(s) 340, 592, 2380, 2411
TaiI ACGT 6 cut(s) 606, 1255, 2471, 2803, 2940, 2955
TaqI TCGA 8 cut(s) 553, 723, 730, 792, 892, 1452, 2866, 3002
TaqII GACCGA 1 cut(s) 955
TatI WGTACW 6 cut(s) 510, 799, 1006, 1040, 1414, 1594
TauI GCSGC 2 cut(s) 1090, 1592
TfiI GAWTC 3 cut(s) 359, 2524, 2587
TscAI CASTG 8 cut(s) 250, 595, 1110, 1587, 1648, 1732, 1749, 2432
TseFI GTSAC 3 cut(s) 1160, 2159, 2759
TseI GCWGC 8 cut(s) 743, 1090, 1093, 1682, 1960, 2245, 2414, 2417
Tsp45I GTSAC 3 cut(s) 1160, 2159, 2759
TspGWI ACGGA 2 cut(s) 1546, 2215
TspRI CASTG 8 cut(s) 250, 595, 1110, 1587, 1648, 1732, 1749, 2432
Tth111I GACNNNGTC 1 cut(s) 2165
Vha464I CTTAAG 2 cut(s) 8, 994
VpaK11BI GGWCC 5 cut(s) 562, 1192, 2347, 2853, 2941
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 8 cut(s) 305, 914, 1001, 1764, 1892, 2573, 2678, 3004
XceI RCATGY 1 cut(s) 1333
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 2 cut(s) 552, 2865
XmnI GAANNNNTTC 3 cut(s) 339, 898, 2117
XspI CTAG 5 cut(s) 144, 1010, 1418, 2259, 2696
ZrmI AGTACT 2 cut(s) 512, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.