FvH4_6g24731

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000756
Physical Location & Seq
Forward (+)
18736637 .. 18743688
7052 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24731.t1

Sequence Viewer

Length: 1236 bp
ATGCTGATTGTAAGTGTAATATACAATGCCAGGAAACATGTTGCAGGTCCTTCCCCTATGCAGACTTGGCATACCAACCTATTGAATGTCCAAGAAGCTCTCCAATCTTGGAGTAAAACGAAATTTCCAAACACTAATGCTCAGCTATACACAACCACAGGTCCACAACAGTGGGAAAAGGTGCTCGACTTCATTGATCCCCTAGTTACTAAGGAGATGAATGAGACTCTCAATGTCCCTATCACCATGGAGGAAGTTCAAGCTGCTACATTTAATCTTGGTGCTCTGAAAGCTCCAGGCCCAGATGGTTTCTCTGGCGTTTTTCTCAAATTCCATCCAAGCAAAAGATCTTTGTTTCCATTGCGCCAAAGCGGGGCTACGGGAGGTCTACATATGGATAAGTCAGTTCATATTTGTTTGAGAGCTGAGGTTTTTGTCATTGCCGCCGGTAGTCCGATGAATGAGTTGAAGCTCTGGCAGAGGTTAATCGCGAGCAGAATTCGGATTTCAAAGACGGTAATTTGGTCGCGGATTGACCTTGAATCGGATAAGGGAAGCTACAGGACTCTATTGAAATCCTTGTGTCAGAGAGGTGAATTGAACAAAGATAAAGAGTTGTTGAGATTGATGACGGGCAAAGTTGGTGACTGGGTTTACCCTCGTTTGCACCCTTACCGGCAAAGCTCATTGGCAAAGCACCATTCCCATAAACTCTCACTGAAGTATTTCAGCCCATTCTTGGTTTTGGAGCGCATTGGTGCTTCTCTTCCAACTTCCCAAATATTACCTCTTGTCTATGACAATGGTTCTTTCAGTTGGATTCTTGCCAAGGTGTTAGATTTGGCAACGCGAAAAGAGCAAAAGAAATCAGTGTCTTACTGGTTAATTAAATGGAAGGGGATGCCGGAGGACGATGCAACTTGGGAATCGGCATCGGCGATAGTGGATTTGCCACGGTTCAATGGTGAAGATGCATTAGGATGGCTAACAATGGCAGAGAGGTACTTGCGTTCTCAACGCGTTCCTCCCTTGGAGTATATACCCACGGTAGCCTCTCATTTCGGCCCAGATGCTTGCAGCGTCACAGACATTCAAACTGCATTGGCATGTTTAGAGCAGACTACAACTGTAGATGCTTTTATTGTTGCATTTACAAAACTTTCAAGCAGAGCTTACGGATGGTCTGAAGCACAACTCTTACCAAATTTTCTGGGAGGACTCAAACCAGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

412

Amino Acids

46.24

Weight (kDa)

9.23

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 277 - 316 4.6e-07 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 388
AccII CGCG 4 cut(s) 491, 529, 850, 1020
AciI CCGC 3 cut(s) 372, 444, 529
AclWI GGATC 1 cut(s) 191
AcsI RAATTY 4 cut(s) 122, 329, 498, 1204
AcuI CTGAAG 2 cut(s) 740, 1206
AfaI GTAC 1 cut(s) 1004
AfiI CCNNNNNNNGG 3 cut(s) 373, 544, 739
AflIII ACRYGT 2 cut(s) 37, 1018
AjnI CCWGG 2 cut(s) 29, 295
AleI CACNNNNGTG 1 cut(s) 169
AluBI AGCT 9 cut(s) 98, 145, 263, 293, 425, 472, 558, 684, 1172
AluI AGCT 9 cut(s) 98, 145, 263, 293, 425, 472, 558, 684, 1172
Alw21I GWGCWC 2 cut(s) 186, 286
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 1 cut(s) 191
AoxI GGCC 2 cut(s) 298, 1063
ApeKI GCWGC 2 cut(s) 263, 1077
ApoI RAATTY 4 cut(s) 122, 329, 498, 1204
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 2 cut(s) 366, 753
AspS9I GGNCC 4 cut(s) 47, 161, 299, 1064
AsuHPI GGTGA 4 cut(s) 235, 605, 656, 977
AvaII GGWCC 2 cut(s) 47, 161
BaeI ACNNNNGTAYC 2 cut(s) 994, 1027
Bbv12I GWGCWC 2 cut(s) 186, 286
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 250, 1089
BccI CCATC 4 cut(s) 299, 342, 975, 1173
BciT130I CCWGG 2 cut(s) 31, 297
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 2 cut(s) 559, 1128
BfuAI ACCTGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 347
BisI GCNGC 3 cut(s) 264, 444, 1078
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 3 cut(s) 265, 445, 1079
Bme1390I CCNGG 2 cut(s) 31, 297
Bme18I GGWCC 2 cut(s) 47, 161
BmgT120I GGNCC 4 cut(s) 47, 161, 299, 1064
BmrFI CCNGG 2 cut(s) 31, 297
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 6 cut(s) 891, 904, 941, 961, 1060, 1123
BmuI ACTGGG 1 cut(s) 658
BpmI CTGGAG 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 141
BsaJI CCNNGG 5 cut(s) 246, 828, 953, 1029, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 544, 739
Bse118I RCCGGY 2 cut(s) 446, 675
Bse1I ACTGG 2 cut(s) 653, 884
Bse3DI GCAATG 2 cut(s) 359, 438
BseBI CCWGG 2 cut(s) 31, 297
BseDI CCNNGG 5 cut(s) 246, 828, 953, 1029, 1044
BseGI GGATG 4 cut(s) 334, 906, 986, 1184
BseLI CCNNNNNNNGG 3 cut(s) 373, 544, 739
BseMI GCAATG 2 cut(s) 359, 438
BseMII CTCAG 2 cut(s) 155, 417
BseNI ACTGG 2 cut(s) 653, 884
BseXI GCAGC 2 cut(s) 250, 1089
Bsh1236I CGCG 4 cut(s) 491, 529, 850, 1020
BshFI GGCC 2 cut(s) 300, 1065
BsiHKAI GWGCWC 2 cut(s) 186, 286
BsiSI CCGG 3 cut(s) 447, 676, 905
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 373, 544, 739
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 2 cut(s) 300, 1065
Bsp1286I GDGCHC 2 cut(s) 186, 286
Bsp143I GATC 2 cut(s) 196, 347
Bsp1720I GCTNAGC 1 cut(s) 141
Bsp19I CCATGG 1 cut(s) 246
Bsp68I TCGCGA 1 cut(s) 491
BspACI CCGC 3 cut(s) 372, 444, 529
BspANI GGCC 2 cut(s) 300, 1065
BspCNI CTCAG 2 cut(s) 154, 418
BspFNI CGCG 4 cut(s) 491, 529, 850, 1020
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 359, 438
BsrFI RCCGGY 2 cut(s) 446, 675
BsrI ACTGG 2 cut(s) 653, 884
BssAI RCCGGY 2 cut(s) 446, 675
BssECI CCNNGG 5 cut(s) 246, 828, 953, 1029, 1044
BssMI GATC 2 cut(s) 196, 347
BssT1I CCWWGG 3 cut(s) 246, 828, 1029
Bst2UI CCWGG 2 cut(s) 31, 297
Bst4CI ACNGT 5 cut(s) 171, 517, 957, 1048, 1129
Bst6I CTCTTC 1 cut(s) 771
BstC8I GCNNGC 2 cut(s) 493, 1075
BstDEI CTNAG 3 cut(s) 141, 210, 426
BstDSI CCRYGG 3 cut(s) 246, 953, 1044
BstF5I GGATG 4 cut(s) 334, 906, 986, 1184
BstFNI CGCG 4 cut(s) 491, 529, 850, 1020
BstHHI GCGC 2 cut(s) 366, 753
BstKTI GATC 2 cut(s) 199, 350
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 2 cut(s) 196, 347
BstMWI GCNNNNNNNGC 3 cut(s) 67, 290, 856
BstNI CCWGG 2 cut(s) 31, 297
BstNSI RCATGY 2 cut(s) 41, 1110
BstSCI CCNGG 2 cut(s) 29, 295
BstSFI CTRYAG 2 cut(s) 559, 1128
BstUI CGCG 4 cut(s) 491, 529, 850, 1020
BstV1I GCAGC 2 cut(s) 250, 1089
BstX2I RGATCY 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 1 cut(s) 347
BsuRI GGCC 2 cut(s) 300, 1065
BtgI CCRYGG 3 cut(s) 246, 953, 1044
BtsCI GGATG 4 cut(s) 334, 906, 986, 1184
BtsIMutI CAGTG 3 cut(s) 176, 716, 876
BtuMI TCGCGA 1 cut(s) 491
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 493, 1075
CfoI GCGC 2 cut(s) 366, 753
Cfr10I RCCGGY 2 cut(s) 446, 675
Cfr13I GGNCC 4 cut(s) 47, 161, 299, 1064
CseI GACGC 1 cut(s) 1069
Csp6I GTAC 1 cut(s) 1003
CviAII CATG 3 cut(s) 38, 247, 1107
CviQI GTAC 1 cut(s) 1003
DdeI CTNAG 3 cut(s) 141, 210, 426
DpnI GATC 2 cut(s) 198, 349
DpnII GATC 2 cut(s) 196, 347
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Eco130I CCWWGG 3 cut(s) 246, 828, 1029
Eco47I GGWCC 2 cut(s) 47, 161
Eco57I CTGAAG 2 cut(s) 740, 1206
EcoO109I RGGNCCY 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 29, 295
EcoT14I CCWWGG 3 cut(s) 246, 828, 1029
EcoT22I ATGCAT 1 cut(s) 976
ErhI CCWWGG 3 cut(s) 246, 828, 1029
FaeI CATG 3 cut(s) 41, 250, 1110
FalI AAGNNNNNCTT 2 cut(s) 1156, 1188
FaqI GGGAC 1 cut(s) 221
FatI CATG 3 cut(s) 37, 246, 1106
FauI CCCGC 1 cut(s) 365
FauNDI CATATG 1 cut(s) 393
FblI GTMKAC 1 cut(s) 388
Fnu4HI GCNGC 3 cut(s) 264, 444, 1078
FokI GGATG 4 cut(s) 321, 913, 993, 1191
Fsp4HI GCNGC 3 cut(s) 264, 444, 1078
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 365, 752
GluI GCNGC 3 cut(s) 264, 444, 1078
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 2 cut(s) 300, 1065
HapII CCGG 3 cut(s) 447, 676, 905
HgaI GACGC 1 cut(s) 1069
HhaI GCGC 2 cut(s) 366, 753
Hin1II CATG 3 cut(s) 41, 250, 1110
Hin6I GCGC 2 cut(s) 364, 751
HinP1I GCGC 2 cut(s) 364, 751
HinfI GANTC 6 cut(s) 226, 542, 565, 820, 926, 1218
HpaII CCGG 3 cut(s) 447, 676, 905
HphI GGTGA 4 cut(s) 235, 605, 656, 977
Hpy166II GTNNAC 3 cut(s) 164, 389, 655
Hpy188I TCNGA 6 cut(s) 288, 456, 504, 547, 588, 1186
Hpy188III TCNNGA 1 cut(s) 490
Hpy8I GTNNAC 3 cut(s) 164, 389, 655
HpyAV CCTTC 2 cut(s) 60, 889
HpyCH4III ACNGT 5 cut(s) 171, 517, 957, 1048, 1129
HpyCH4V TGCA 8 cut(s) 44, 61, 667, 917, 974, 1077, 1100, 1148
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 290, 856
HpyF3I CTNAG 3 cut(s) 141, 210, 426
Hsp92II CATG 3 cut(s) 41, 250, 1110
HspAI GCGC 2 cut(s) 364, 751
Kzo9I GATC 2 cut(s) 196, 347
LmnI GCTCC 2 cut(s) 298, 748
Lsp1109I GCAGC 2 cut(s) 250, 1089
LweI GCATC 6 cut(s) 891, 904, 941, 961, 1060, 1123
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 3 cut(s) 205, 644, 1081
MalI GATC 2 cut(s) 198, 349
MboI GATC 2 cut(s) 196, 347
MboII GAAGA 2 cut(s) 758, 980
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 2 cut(s) 186, 286
MluCI AATT 7 cut(s) 122, 329, 498, 519, 596, 885, 1204
MluI ACGCGT 1 cut(s) 1018
MlyI GAGTC 3 cut(s) 220, 559, 1212
MmeI TCCRAC 2 cut(s) 794, 797
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 4 cut(s) 273, 485, 884, 888
MslI CAYNNNNRTG 3 cut(s) 169, 979, 1105
MspI CCGG 3 cut(s) 447, 676, 905
MspR9I CCNGG 2 cut(s) 31, 297
MvaI CCWGG 2 cut(s) 31, 297
MvnI CGCG 4 cut(s) 491, 529, 850, 1020
MwoI GCNNNNNNNGC 3 cut(s) 67, 290, 856
NcoI CCATGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 393
NdeII GATC 2 cut(s) 196, 347
NlaIII CATG 3 cut(s) 41, 250, 1110
NmuCI GTSAC 2 cut(s) 644, 1081
NruI TCGCGA 1 cut(s) 491
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 2 cut(s) 41, 1110
OliI CACNNNNGTG 1 cut(s) 169
PacI TTAATTAA 1 cut(s) 888
PciI ACATGT 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 3 cut(s) 542, 820, 926
PkrI GCNGC 3 cut(s) 265, 445, 1079
PleI GAGTC 3 cut(s) 220, 559, 1212
PpsI GAGTC 3 cut(s) 220, 559, 1212
PpuMI RGGWCCY 1 cut(s) 47
PscI ACATGT 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 29, 295
PspGI CCWGG 2 cut(s) 29, 295
PspPI GGNCC 4 cut(s) 47, 161, 299, 1064
PspPPI RGGWCCY 1 cut(s) 47
PsuI RGATCY 1 cut(s) 347
RruI TCGCGA 1 cut(s) 491
RsaI GTAC 1 cut(s) 1004
RsaNI GTAC 1 cut(s) 1003
RseI CAYNNNNRTG 3 cut(s) 169, 979, 1105
SaqAI TTAA 4 cut(s) 273, 485, 884, 888
SatI GCNGC 3 cut(s) 264, 444, 1078
Sau3AI GATC 2 cut(s) 196, 347
Sau96I GGNCC 4 cut(s) 47, 161, 299, 1064
SchI GAGTC 3 cut(s) 220, 559, 1212
ScrFI CCNGG 2 cut(s) 31, 297
SduI GDGCHC 2 cut(s) 186, 286
SfaNI GCATC 6 cut(s) 891, 904, 941, 961, 1060, 1123
SfcI CTRYAG 2 cut(s) 559, 1128
SinI GGWCC 2 cut(s) 47, 161
SmiMI CAYNNNNRTG 3 cut(s) 169, 979, 1105
Sse9I AATT 7 cut(s) 122, 329, 498, 519, 596, 885, 1204
SsiI CCGC 3 cut(s) 372, 444, 529
SspI AATATT 1 cut(s) 783
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 29, 295
StyI CCWWGG 3 cut(s) 246, 828, 1029
TaaI ACNGT 5 cut(s) 171, 517, 957, 1048, 1129
TaqI TCGA 1 cut(s) 186
TasI AATT 7 cut(s) 122, 329, 498, 519, 596, 885, 1204
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 3 cut(s) 542, 820, 926
Tru1I TTAA 4 cut(s) 273, 485, 884, 888
Tru9I TTAA 4 cut(s) 273, 485, 884, 888
TscAI CASTG 3 cut(s) 176, 723, 876
TseFI GTSAC 2 cut(s) 644, 1081
TseI GCWGC 2 cut(s) 263, 1077
Tsp45I GTSAC 2 cut(s) 644, 1081
TspDTI ATGAA 4 cut(s) 181, 233, 398, 473
TspGWI ACGGA 1 cut(s) 1191
TspRI CASTG 3 cut(s) 176, 723, 876
VpaK11BI GGWCC 2 cut(s) 47, 161
XapI RAATTY 4 cut(s) 122, 329, 498, 1204
XceI RCATGY 2 cut(s) 41, 1110
XmiI GTMKAC 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.