Rroxscaffold_1G00071870

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000756
Physical Location & Seq
Reverse (-)
93015004 .. 93020942
5939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071870.1

Sequence Viewer

Length: 2697 bp
ATGATGAATGAGCTCAGATCCATGAAGGATAGCTCGGTTGCAACTGCTGCTCACCTCGATTCTTCAATCCTTAAGTTTGGATCCCTACCTCCTACATCCGGCTCACCGGGTTTAGCAGCACGAGCGAACTCAGCCTCCTCCACAAGAGCCAACACAACAAAGGCCATCCTGCCTACTGATGGTAGTATATCTCTTCCTCTACATACCTTCTCCTTAAATCCTCTCAACCAAGAAGTACCTATTGCGGTTACCAGCGAGATTCCCACTGAGACCATTAATGCATCTGTTGCTTTGGGGTCCTCTAGGGCCTTAATGACTAATGCATCTGGGTCATTGATACCAAACCCCGTTGATTTAGATGGTGGTGATAAGGAGGAAATTAGTTTACATCGCTCTCAAGAGAGGGCTGAGCAATCACAAACCCTTTACTATCAACATTCTAATTTTGGATCTCAACCTTTCAATCATTTTGCTGGACCATATGTGACACCTAATAGCTATCAGTTCTTTGCCAGTCCACAGTTCCACCAAATTCCATCAAGCAATAGTTTGGATCCATTTACTATGACCTATTCAGGACCTTACTCATACACTCCCATGAACTTTGTGAACGCACCCACACAACCACCGCAGTCATCAACAATTCAAGGCTACCCTAGCTCTTATATTCCACAATCCATGGGGACAGCTCAACCTACATTTGCCACTTCCACAACATCCATGCCTCACTACAGCTATACACCCTACATATCTCAGTCACACCATACCCACACTGTGGCTCCAAGTTCACCAAACCAACAGCCACATGGATATCCACCCCGACAATTCCATCAGCAAGTTCCTCACTCTTTTGATCCAAACCTACCTACCATGAAGCAAATGAAGTTGGAATTCAACGTTTTCAGCGGCGAGGACCCGGTTGAGTGGTTGAACAAGGCAGAGCAATATTTTGATTTTTATCAAATACCAAATGACAGAAAGTTAGCTATTGCTACTATGCACTTAACAGAGAAAGCTTCTGATAGATGGTTCATGTTCAAGCATGAGTTCCCAAATACATGGCCAGGATTAGCTGATCTATTGATGAGAGAGTTCAGTGGGCACAATGTGATTGATTACCAAGCCACCTTAGCCAGGTCTCAAGTAGGAAGTGTGGGACAATACATTGACCAATTCACTAGACTGTCCAGAAGAGCTCCAGGTTTCTCTCAACAGGTGCTGCTATCTTGTTTCCTAGGGGGACTTAAGGATAACATAAGAGCCGAAGTCAAGGCTCAGAAGCCAAAAACACTGTATGAGGCTTGTGAGTTAGCCAAAATCTATGAGGAGAGAGAATTAAAGAAACAACAAGCGAGAGGAACTTTTGTTAACAGAAACAACACTGGGGTCCAACATAGAAATGTTAATGCAGGAGGTCAAGCTCGGGGTATTCCACTAGCCAATCTAGCTAGACCACAAGCTGCCCAACCAGTGGGGGCAGTCAATCAAGTTGGTGGAAATAGGAGGTTAACACAAGATGAATATCAAGAGAGGAGAGCAAGAAACCAATGTTTCTTCTGTGATGATATTTTTAGGCCAGGCCACAATTGCAGAAGAGGTCAAGCAATGTTGATAATTGAAGTGCTACAAGATGAGGTGAACATGCAGGGTCCAATTCATGATGAGGAGTTGGAGGAAGCTCATCCAACTACAGAAGAAGTTGAAAATCGGGACGTGGAATTACAAGTCATGGGAGATGGGAGCAGTACATCAACCGTGCAACTCAAGGGAGAAATCAACCACAGAATTGCACATGTATTAATAGATTCGGGTGCATCCCACAACTTCATTCACCCTGCACTCATAAAAAAATCAAAGACCACTGTTCATTCCATCAACCCTCTCAGAGTAAGGCTTGCTAGTGGGGCAATCATGCACACTAAAGGGCAGGTGAGGCTTGATCTGTGTTCACAACAATTCCATTTTTCTGCTGATTTCTATGTCTTACCTGTTTCAAGATGTGAAATAGTTTTGGGGGCCACTTGGCTAAGAACCTTGGGGGACATAACCTGGAATTTTGATACGATGAGAATGAGGTTTCACAACAAGGGCAAGGAATATTTGTTGCAGGGTGAAACTGAAACACAAGCTGCAGTTGTAGGCTGCAAAGTTATGTCTCGGATACTCAAAAAAGAGAGAGAAGCCATCTTGGTCCAGCTTAATCAGATAACCACTCCCCAAGCCAAGGAAGCCATTCATCTTGATATTAAAAGGTTGATACAGAAATATTCTGGAGTATTTGAAACACCAAGATCACTACCACCTGCAAGGCCACAAGACCACAGGATTGAGTTGGTGCCTAACACAGCCCCTATTAATGTTAGGCCTTACAGATATCCACATTTCCAGAAAACAGAAATTGAGAAGATTGTGCAAGAGCTGCTGGATTCAGGAGTTATCAGGCCTAGTGTTAGCCCTTTTTCTTCTCATGTGTTACTAGTTAAGAAGAAAGATGGGACTTGGAGGATGTGTGTTGACTATAGGTCCCTCAATGCAGCTACTATGAAAGATAAGTACCCAATACCAGTGGTGGATGAGTTGATTGATGAGGTACATGGTGCCACTGTGTATACAAAATTGGATCTCCGGTCTGGATACCACCAAATTAGGATGGCAGAACAAGACATAAACAAAACTGCATTTAGAACTCATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

898

Amino Acids

100.78

Weight (kDa)

8.36

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 316 - 442 1.2e-08 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 330 - 416 4.5e-07 Retrotransposon gag protein
RVP_2 PF08284 586 - 677 5.4e-08 Retroviral aspartyl protease
gag-asp_proteas PF13975 589 - 677 5.8e-06 gag-polyprotein putative aspartyl protease
RVT_1 PF00078 827 - 897 4.1e-12 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1918, 2311
Acc36I ACCTGC 2 cut(s) 1918, 2311
AccB1I GGYRCC 2 cut(s) 2335, 2597
AccB7I CCANNNNNTGG 2 cut(s) 775, 1471
AccI GTMKAC 1 cut(s) 2609
AciI CCGC 3 cut(s) 245, 629, 906
AclI AACGTT 1 cut(s) 897
AclWI GGATC 8 cut(s) 12, 75, 88, 457, 548, 561, 848, 2628
AcoI YGGCCR 1 cut(s) 1061
AcsI RAATTY 3 cut(s) 531, 890, 2053
AdeI CACNNNGTG 2 cut(s) 775, 1108
AfaI GTAC 4 cut(s) 237, 1747, 2555, 2592
AfiI CCNNNNNNNGG 6 cut(s) 98, 179, 775, 1134, 1471, 2224
AflII CTTAAG 2 cut(s) 71, 1244
AflIII ACRYGT 1 cut(s) 1792
AhdI GACNNNNNGTC 1 cut(s) 2521
AhlI ACTAGT 1 cut(s) 2476
AjiI CACGTC 1 cut(s) 1714
AjnI CCWGG 5 cut(s) 1063, 1133, 1198, 1576, 2048
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
Alw21I GWGCWC 2 cut(s) 15, 1198
Alw26I GTCTC 3 cut(s) 263, 1143, 2160
AlwI GGATC 8 cut(s) 12, 75, 88, 457, 548, 561, 848, 2628
AlwNI CAGNNNCTG 1 cut(s) 1219
Ama87I CYCGRG 1 cut(s) 1422
AoxI GGCC 9 cut(s) 162, 306, 1061, 1574, 1579, 2016, 2309, 2363, 2441
ApeKI GCWGC 8 cut(s) 47, 116, 1219, 1460, 2129, 2142, 2419, 2534
ApoI RAATTY 3 cut(s) 531, 890, 2053
AseI ATTAAT 3 cut(s) 276, 1799, 2355
Asp700I GAANNNNTTC 2 cut(s) 2232, 2689
AspA2I CCTAGG 1 cut(s) 1234
AsuC2I CCSGG 2 cut(s) 108, 917
AsuHPI GGTGA 8 cut(s) 44, 96, 377, 780, 1648, 1823, 1942, 2123
AvaI CYCGRG 1 cut(s) 1422
AvaII GGWCC 8 cut(s) 297, 476, 578, 913, 1387, 1649, 2191, 2523
AvrII CCTAGG 1 cut(s) 1234
BaeGI GKGCMC 1 cut(s) 1104
BalI TGGCCA 1 cut(s) 1063
BamHI GGATCC 2 cut(s) 80, 553
BanI GGYRCC 2 cut(s) 2335, 2597
BanII GRGCYC 2 cut(s) 15, 1198
BauI CACGAG 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 15, 1198
BbvI GCAGC 8 cut(s) 34, 128, 1206, 1447, 2116, 2129, 2406, 2546
BciT130I CCWGG 5 cut(s) 1065, 1135, 1200, 1578, 2050
BciVI GTATCC 2 cut(s) 2154, 2627
BcnI CCSGG 2 cut(s) 108, 917
BcoDI GTCTC 3 cut(s) 263, 1143, 2160
BcuI ACTAGT 1 cut(s) 2476
BfmI CTRYAG 4 cut(s) 730, 1689, 2130, 2518
BfrI CTTAAG 2 cut(s) 71, 1244
BfuAI ACCTGC 2 cut(s) 1918, 2311
BfuI GTATCC 2 cut(s) 2154, 2627
BisI GCNGC 9 cut(s) 48, 117, 907, 1220, 1461, 2130, 2143, 2420, 2535
BlnI CCTAGG 1 cut(s) 1234
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 9 cut(s) 49, 118, 908, 1221, 1462, 2131, 2144, 2421, 2536
Bme1390I CCNGG 7 cut(s) 108, 917, 1065, 1135, 1200, 1578, 2050
Bme18I GGWCC 8 cut(s) 297, 476, 578, 913, 1387, 1649, 2191, 2523
BmeRI GACNNNNNGTC 1 cut(s) 2521
BmeT110I CYCGRG 1 cut(s) 1422
BmgBI CACGTC 1 cut(s) 1714
BmrFI CCNGG 7 cut(s) 108, 917, 1065, 1135, 1200, 1578, 2050
BmrI ACTGGG 1 cut(s) 1392
BmsI GCATC 3 cut(s) 290, 332, 1823
BmuI ACTGGG 1 cut(s) 1392
BpmI CTGGAG 2 cut(s) 1182, 2292
Bpu10I CCTNAGC 1 cut(s) 1129
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 381, 1125, 1748
BpuMI CCSGG 2 cut(s) 108, 917
BsaBI GATNNNNATC 2 cut(s) 957, 1521
BsaI GGTCTC 2 cut(s) 263, 1143
BsaJI CCNNGG 4 cut(s) 678, 1234, 2034, 2223
BsaWI WCCGGW 1 cut(s) 2625
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 763, 793, 2494, 2524
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 179, 775, 1134, 1471, 2224
Bse1I ACTGG 4 cut(s) 513, 1387, 1469, 2564
Bse3DI GCAATG 1 cut(s) 1611
Bse8I GATNNNNATC 2 cut(s) 957, 1521
BseBI CCWGG 5 cut(s) 1065, 1135, 1200, 1578, 2050
BseDI CCNNGG 4 cut(s) 678, 1234, 2034, 2223
BseGI GGATG 8 cut(s) 95, 165, 716, 1681, 1814, 2511, 2578, 2655
BseJI GATNNNNATC 2 cut(s) 957, 1521
BseLI CCNNNNNNNGG 6 cut(s) 98, 179, 775, 1134, 1471, 2224
BseMI GCAATG 1 cut(s) 1611
BseMII CTCAG 7 cut(s) 28, 144, 258, 399, 767, 1289, 1897
BseNI ACTGG 4 cut(s) 513, 1387, 1469, 2564
BseRI GAGGAG 4 cut(s) 127, 1340, 1546, 1679
BseSI GKGCMC 1 cut(s) 1104
BseXI GCAGC 8 cut(s) 34, 128, 1206, 1447, 2116, 2129, 2406, 2546
BsgI GTGCAG 1 cut(s) 1821
BshFI GGCC 9 cut(s) 164, 308, 1063, 1576, 1581, 2018, 2311, 2365, 2443
BshNI GGYRCC 2 cut(s) 2335, 2597
BsiHKAI GWGCWC 2 cut(s) 15, 1198
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 4 cut(s) 99, 107, 917, 2626
BslFI GGGAC 7 cut(s) 697, 1170, 1254, 1724, 2054, 2509, 2509
BslI CCNNNNNNNGG 6 cut(s) 98, 179, 775, 1134, 1471, 2224
BsmAI GTCTC 3 cut(s) 263, 1143, 2160
BsmFI GGGAC 7 cut(s) 697, 1170, 1254, 1724, 2054, 2509, 2509
BsnI GGCC 9 cut(s) 164, 308, 1063, 1576, 1581, 2018, 2311, 2365, 2443
Bso31I GGTCTC 2 cut(s) 263, 1143
BsoBI CYCGRG 1 cut(s) 1422
Bsp1286I GDGCHC 3 cut(s) 15, 1104, 1198
Bsp143I GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 3 cut(s) 245, 629, 906
BspANI GGCC 9 cut(s) 164, 308, 1063, 1576, 1581, 2018, 2311, 2365, 2443
BspCNI CTCAG 7 cut(s) 27, 143, 259, 400, 766, 1288, 1896
BspHI TCATGA 1 cut(s) 1657
BspMAI CTGCAG 1 cut(s) 2134
BspMI ACCTGC 2 cut(s) 1918, 2311
BspPI GGATC 8 cut(s) 12, 75, 88, 457, 548, 561, 848, 2628
BspQI GCTCTTC 1 cut(s) 1186
BspT107I GGYRCC 2 cut(s) 2335, 2597
BspTI CTTAAG 2 cut(s) 71, 1244
BspTNI GGTCTC 2 cut(s) 263, 1143
BsrDI GCAATG 1 cut(s) 1611
BsrI ACTGG 4 cut(s) 513, 1387, 1469, 2564
BssECI CCNNGG 4 cut(s) 678, 1234, 2034, 2223
BssMI GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
BssNAI GTATAC 1 cut(s) 2610
BssSI CACGAG 1 cut(s) 120
BssT1I CCWWGG 4 cut(s) 678, 1234, 2034, 2223
Bst1107I GTATAC 1 cut(s) 2610
Bst2BI CACGAG 1 cut(s) 120
Bst2UI CCWGG 5 cut(s) 1065, 1135, 1200, 1578, 2050
Bst4CI ACNGT 7 cut(s) 522, 775, 1185, 1293, 1756, 1864, 2605
Bst6I CTCTTC 3 cut(s) 198, 1186, 1588
BstAFI CTTAAG 2 cut(s) 71, 1244
BstAPI GCANNNNNTGC 3 cut(s) 47, 287, 2419
BstC8I GCNNGC 1 cut(s) 1896
BstDEI CTNAG 9 cut(s) 14, 130, 267, 408, 753, 1129, 1275, 1883, 2027
BstDSI CCRYGG 1 cut(s) 678
BstEII GGTNACC 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 1132
BstF5I GGATG 8 cut(s) 95, 165, 716, 1681, 1814, 2511, 2578, 2655
BstKTI GATC 9 cut(s) 20, 83, 452, 556, 856, 1078, 1942, 2294, 2623
BstMAI GTCTC 3 cut(s) 263, 1143, 2160
BstMBI GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
BstNI CCWGG 5 cut(s) 1065, 1135, 1200, 1578, 2050
BstNSI RCATGY 2 cut(s) 1645, 1796
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 7 cut(s) 106, 915, 1063, 1133, 1198, 1576, 2048
BstSFI CTRYAG 4 cut(s) 730, 1689, 2130, 2518
BstSLI GKGCMC 1 cut(s) 1104
BstV1I GCAGC 8 cut(s) 34, 128, 1206, 1447, 2116, 2129, 2406, 2546
BstX2I RGATCY 5 cut(s) 17, 80, 449, 553, 2620
BstXI CCANNNNNNTGG 1 cut(s) 1059
BstYI RGATCY 5 cut(s) 17, 80, 449, 553, 2620
BstZ17I GTATAC 1 cut(s) 2610
BsuI GTATCC 2 cut(s) 2154, 2627
BsuRI GGCC 9 cut(s) 164, 308, 1063, 1576, 1581, 2018, 2311, 2365, 2443
BtgI CCRYGG 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 374
BtrI CACGTC 1 cut(s) 1714
BtsCI GGATG 8 cut(s) 95, 165, 716, 1681, 1814, 2511, 2578, 2655
BtsIMutI CAGTG 9 cut(s) 264, 771, 1102, 1289, 1380, 1476, 1860, 2571, 2601
BveI ACCTGC 2 cut(s) 1918, 2311
Cac8I GCNNGC 1 cut(s) 1896
CaiI CAGNNNCTG 1 cut(s) 1219
CciI TCATGA 1 cut(s) 1657
Csp6I GTAC 4 cut(s) 236, 1746, 2554, 2591
CspCI CAANNNNNGTGG 2 cut(s) 2547, 2582
CviQI GTAC 4 cut(s) 236, 1746, 2554, 2591
DdeI CTNAG 9 cut(s) 14, 130, 267, 408, 753, 1129, 1275, 1883, 2027
DpnI GATC 9 cut(s) 19, 82, 451, 555, 855, 1077, 1941, 2293, 2622
DpnII GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
DraIII CACNNNGTG 2 cut(s) 775, 1108
DriI GACNNNNNGTC 1 cut(s) 2521
EaeI YGGCCR 1 cut(s) 1061
Eam1104I CTCTTC 3 cut(s) 198, 1186, 1588
Eam1105I GACNNNNNGTC 1 cut(s) 2521
EarI CTCTTC 3 cut(s) 198, 1186, 1588
Ecl136II GAGCTC 2 cut(s) 13, 1196
Eco130I CCWWGG 4 cut(s) 678, 1234, 2034, 2223
Eco147I AGGCCT 2 cut(s) 2365, 2443
Eco24I GRGCYC 2 cut(s) 15, 1198
Eco31I GGTCTC 2 cut(s) 263, 1143
Eco32I GATATC 2 cut(s) 812, 2375
Eco47I GGWCC 8 cut(s) 297, 476, 578, 913, 1387, 1649, 2191, 2523
Eco53kI GAGCTC 2 cut(s) 13, 1196
Eco88I CYCGRG 1 cut(s) 1422
Eco91I GGTNACC 1 cut(s) 247
EcoICRI GAGCTC 2 cut(s) 13, 1196
EcoNI CCTNNNNNAGG 1 cut(s) 1132
EcoO109I RGGNCCY 5 cut(s) 297, 306, 578, 913, 2523
EcoO65I GGTNACC 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 890
EcoRII CCWGG 5 cut(s) 1063, 1133, 1198, 1576, 2048
EcoRV GATATC 2 cut(s) 812, 2375
EcoT14I CCWWGG 4 cut(s) 678, 1234, 2034, 2223
EcoT22I ATGCAT 2 cut(s) 283, 325
EcoT38I GRGCYC 2 cut(s) 15, 1198
ErhI CCWWGG 4 cut(s) 678, 1234, 2034, 2223
FaqI GGGAC 7 cut(s) 697, 1170, 1254, 1724, 2054, 2509, 2509
FauNDI CATATG 1 cut(s) 481
FblI GTMKAC 1 cut(s) 2609
Fnu4HI GCNGC 9 cut(s) 48, 117, 907, 1220, 1461, 2130, 2143, 2420, 2535
FokI GGATG 8 cut(s) 82, 152, 703, 1668, 1801, 2518, 2585, 2662
FriOI GRGCYC 2 cut(s) 15, 1198
Fsp4HI GCNGC 9 cut(s) 48, 117, 907, 1220, 1461, 2130, 2143, 2420, 2535
GluI GCNGC 9 cut(s) 48, 117, 907, 1220, 1461, 2130, 2143, 2420, 2535
GsuI CTGGAG 2 cut(s) 1182, 2292
HaeIII GGCC 9 cut(s) 164, 308, 1063, 1576, 1581, 2018, 2311, 2365, 2443
HapII CCGG 4 cut(s) 99, 107, 917, 2626
HincII GTYRAC 3 cut(s) 1369, 1509, 2515
HindII GTYRAC 3 cut(s) 1369, 1509, 2515
HindIII AAGCTT 1 cut(s) 1014
HinfI GANTC 4 cut(s) 59, 259, 1805, 2426
HpaI GTTAAC 2 cut(s) 1369, 1509
HpaII CCGG 4 cut(s) 99, 107, 917, 2626
HphI GGTGA 8 cut(s) 44, 96, 377, 780, 1648, 1823, 1942, 2123
Hpy188I TCNGA 6 cut(s) 17, 1021, 1278, 1886, 2160, 2205
HpyAV CCTTC 2 cut(s) 19, 217
HpyCH4III ACNGT 7 cut(s) 522, 775, 1185, 1293, 1756, 1864, 2605
HpyCH4IV ACGT 2 cut(s) 897, 1713
HpyF3I CTNAG 9 cut(s) 14, 130, 267, 408, 753, 1129, 1275, 1883, 2027
HpySE526I ACGT 2 cut(s) 897, 1713
KspAI GTTAAC 2 cut(s) 1369, 1509
Kzo9I GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
LguI GCTCTTC 1 cut(s) 1186
LmnI GCTCC 3 cut(s) 784, 1201, 1740
Lsp1109I GCAGC 8 cut(s) 34, 128, 1206, 1447, 2116, 2129, 2406, 2546
LweI GCATC 3 cut(s) 290, 332, 1823
MaeII ACGT 2 cut(s) 897, 1713
MaeIII GTNAC 4 cut(s) 247, 484, 756, 2472
MalI GATC 9 cut(s) 19, 82, 451, 555, 855, 1077, 1941, 2293, 2622
MboI GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
MboII GAAGA 9 cut(s) 54, 185, 1203, 1546, 1605, 1706, 2416, 2454, 2497
MfeI CAATTG 1 cut(s) 1585
MflI RGATCY 5 cut(s) 17, 80, 449, 553, 2620
MhlI GDGCHC 3 cut(s) 15, 1104, 1198
MlsI TGGCCA 1 cut(s) 1063
MluNI TGGCCA 1 cut(s) 1063
MmeI TCCRAC 4 cut(s) 867, 1414, 1650, 1709
Mox20I TGGCCA 1 cut(s) 1063
Mph1103I ATGCAT 2 cut(s) 283, 325
MroXI GAANNNNTTC 2 cut(s) 2232, 2689
MscI TGGCCA 1 cut(s) 1063
MslI CAYNNNNRTG 2 cut(s) 596, 1398
Msp20I TGGCCA 1 cut(s) 1063
MspA1I CMGCKG 1 cut(s) 906
MspCI CTTAAG 2 cut(s) 71, 1244
MspI CCGG 4 cut(s) 99, 107, 917, 2626
MspR9I CCNGG 7 cut(s) 108, 917, 1065, 1135, 1200, 1578, 2050
MunI CAATTG 1 cut(s) 1585
MvaI CCWGG 5 cut(s) 1065, 1135, 1200, 1578, 2050
NciI CCSGG 2 cut(s) 108, 917
NcoI CCATGG 1 cut(s) 678
NdeI CATATG 1 cut(s) 481
NdeII GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
NmuCI GTSAC 2 cut(s) 484, 756
NsiI ATGCAT 2 cut(s) 283, 325
NspI RCATGY 2 cut(s) 1645, 1796
PagI TCATGA 1 cut(s) 1657
PaqCI CACCTGC 2 cut(s) 1918, 2311
PceI AGGCCT 2 cut(s) 2365, 2443
PciI ACATGT 1 cut(s) 1792
PciSI GCTCTTC 1 cut(s) 1186
PdmI GAANNNNTTC 2 cut(s) 2232, 2689
PfeI GAWTC 4 cut(s) 59, 259, 1805, 2426
PflMI CCANNNNNTGG 2 cut(s) 775, 1471
PkrI GCNGC 9 cut(s) 49, 118, 908, 1221, 1462, 2131, 2144, 2421, 2536
PpuMI RGGWCCY 4 cut(s) 297, 578, 913, 2523
PscI ACATGT 1 cut(s) 1792
PshBI ATTAAT 3 cut(s) 276, 1799, 2355
Psp124BI GAGCTC 2 cut(s) 15, 1198
Psp1406I AACGTT 1 cut(s) 897
Psp5II RGGWCCY 4 cut(s) 297, 578, 913, 2523
Psp6I CCWGG 5 cut(s) 1063, 1133, 1198, 1576, 2048
PspEI GGTNACC 1 cut(s) 247
PspGI CCWGG 5 cut(s) 1063, 1133, 1198, 1576, 2048
PspPPI RGGWCCY 4 cut(s) 297, 578, 913, 2523
PstI CTGCAG 1 cut(s) 2134
PstNI CAGNNNCTG 1 cut(s) 1219
PsuI RGATCY 5 cut(s) 17, 80, 449, 553, 2620
RsaI GTAC 4 cut(s) 237, 1747, 2555, 2592
RsaNI GTAC 4 cut(s) 236, 1746, 2554, 2591
RseI CAYNNNNRTG 2 cut(s) 596, 1398
SacI GAGCTC 2 cut(s) 15, 1198
SapI GCTCTTC 1 cut(s) 1186
SatI GCNGC 9 cut(s) 48, 117, 907, 1220, 1461, 2130, 2143, 2420, 2535
Sau3AI GATC 9 cut(s) 17, 80, 449, 553, 853, 1075, 1939, 2291, 2620
ScrFI CCNGG 7 cut(s) 108, 917, 1065, 1135, 1200, 1578, 2050
SduI GDGCHC 3 cut(s) 15, 1104, 1198
SfaNI GCATC 3 cut(s) 290, 332, 1823
SfcI CTRYAG 4 cut(s) 730, 1689, 2130, 2518
SinI GGWCC 8 cut(s) 297, 476, 578, 913, 1387, 1649, 2191, 2523
SmiMI CAYNNNNRTG 2 cut(s) 596, 1398
SmlI CTYRAG 5 cut(s) 71, 396, 1140, 1244, 1763
SmoI CTYRAG 5 cut(s) 71, 396, 1140, 1244, 1763
SpeI ACTAGT 1 cut(s) 2476
SseBI AGGCCT 2 cut(s) 2365, 2443
SsiI CCGC 3 cut(s) 245, 629, 906
SspI AATATT 3 cut(s) 947, 2099, 2267
SstI GAGCTC 2 cut(s) 15, 1198
StuI AGGCCT 2 cut(s) 2365, 2443
StyD4I CCNGG 7 cut(s) 106, 915, 1063, 1133, 1198, 1576, 2048
StyI CCWWGG 4 cut(s) 678, 1234, 2034, 2223
TaaI ACNGT 7 cut(s) 522, 775, 1185, 1293, 1756, 1864, 2605
TaiI ACGT 2 cut(s) 900, 1716
TaqI TCGA 1 cut(s) 57
TatI WGTACW 1 cut(s) 1745
TauI GCSGC 1 cut(s) 909
TfiI GAWTC 4 cut(s) 59, 259, 1805, 2426
TscAI CASTG 9 cut(s) 271, 778, 1102, 1296, 1387, 1476, 1867, 2571, 2608
TseFI GTSAC 2 cut(s) 484, 756
TseI GCWGC 8 cut(s) 47, 116, 1219, 1460, 2129, 2142, 2419, 2534
Tsp45I GTSAC 2 cut(s) 484, 756
TspRI CASTG 9 cut(s) 271, 778, 1102, 1296, 1387, 1476, 1867, 2571, 2608
Van91I CCANNNNNTGG 2 cut(s) 775, 1471
Vha464I CTTAAG 2 cut(s) 71, 1244
VpaK11BI GGWCC 8 cut(s) 297, 476, 578, 913, 1387, 1649, 2191, 2523
VspI ATTAAT 3 cut(s) 276, 1799, 2355
XagI CCTNNNNNAGG 1 cut(s) 1132
XapI RAATTY 3 cut(s) 531, 890, 2053
XceI RCATGY 2 cut(s) 1645, 1796
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XmaJI CCTAGG 1 cut(s) 1234
XmiI GTMKAC 1 cut(s) 2609
XmnI GAANNNNTTC 2 cut(s) 2232, 2689
Zsp2I ATGCAT 2 cut(s) 283, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.