Rw3G002230

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000756
Physical Location & Seq
Forward (+)
1962325 .. 1965775
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G002230.1

Sequence Viewer

Length: 3048 bp
ATGGCCAAACTCAAGGGTGGGGGGTCTAAACCCTCGTCTCCACCGCGTCCTTCCGATGAACCTCACGTTTCTGGTGCACTCTCTCACCTGGAATATGAGCTGCAAGTCCATCGTGAGGAAACTAGTGCTAAATTAGAGGAGATCCAAGCTTCCATACACGATTTCATGGCTGCTTCCTTCGCTAGTTTCAAAACCCTCTTATTGGATGATTTCAAGCTGCAGCTCACCTCCTTCATCAAAGAGACCGGCAGTACGCCTCAACCACAGGCCACTAAGCACGGCGACGTAGCCATCACCCAACCGGACGGATCGGTCCGCTTTGGCTCCTTACCATCTCCGGTGCTCCAACAACGATTGGTGAGCTTCACGGAGTCCTCTTCATCTACTGTGGCCAGATCTGAGGTTAATGCTACTGTGGAGATGACTAATGTGGATACATTCATGGTGGATGGTTCCGCTAGTCGAACCAAGAATGCTTTCCGTCCTCTAGGCTTTAAACATGGGGGGAGCAGCGTGCTTATAGTCACTTCTGTGGGGGAACCTCAGTTTCACCAGGGGCTAGTGTCACAGCCCTTTACCATGACCTCGAGTCCACAGTATGTTGAGGGTTGCTTTGGTTTTGCACAGGTTACGAGCCAACCTATACCCGAGCACAACCCCTTTGTCATGACCTATTCTGGGCCTTACTTAGTTTCCATGATCAATATACCATCCCAAATAGCTCAACCTACCTACTCATCTGTTATGCCACCCTATACATACCCTAAGAGTGCTCCACCTAATTCCACTGTGATGCCTACTTTTGCCACCACTACAACAACCCAGTTGGTTCAACCACCTTATCAACAACAGACTGCTCCCTTTCAAAACTTCTTACAACCCCATCAACCCTATTTGCTACCTCCTTACTATAGTGTTTCACACATTGACCCAAATTTACCTACTATGAAACAGATGAGATTGGAATTCAGTGTGTTAAGTGGGGGAGATCCTGTTGAGTGGCTTAACAAGGCTGATCAATATTTTGAGTTTTATCATATTCCAGAGGATAGAAAGCTTTCCATTGCCACAATGCATTTATCTGATAAAGCTTTAGACAGATGGTACATGTTTAGGCATGAGTTCCTTAATACTTGGCAGGGTCTAGCTGATTTATTGATGAGGGAATTCAGTGGCTACAATAGATCCGAGTATCAAGCTGCCTTGGCAAGGATGAACCAAACTGGTTCAGTGGAAAAAAGGGCTCCAGGATTCCCACCTGAAGTGCTACTGTCCTGCTTTATTGGAGGATTGAAGGAAGAGATTAGGGTGGATGTTCAGGCTCAAAAGCCTAGATCCTTATATGAAGCATGTGAGTTAGCCAGGGTTGGAGCACAAGGCAAATTCACACAAATCACCAATGCTAGTTCTAAACTAGGAAATGCCACATTCAGTTCCAGAGGGTCACAATCACACTCTAGCAATTCTGGAGGGAGCAATAATATCAGGATCAAATTATCTGATGCAGAATTCGAAGCTCGAAGACCGGGCCACAACTGTAGAAAGAAAGGCCAGCTCATGAGCATGGAAATAGTACCCCATGAAAGTGAGTTGGCTGAAGTCCTAAATGAGAGGCCATTGGTGGAAGTACCCCCTATCACAGATTTGGATGAGCCCTTAATTAGGCTACAGGTAATGGGGAATGGCCATTCCAAGACCTCAACTATGCAGTTGAAAGGCAATTTCTATAAGAAAGTGGTTCATGTCTTAATTGATTCTGGAGCAACACATAATTTCATACATCCCCAGCTGCTCAAGGGCACTAAACTCCAAGTACACAAGCTTTCTCCCTTGAATGTAATATTAACTAGTGGAGCTGAAGTACAAACAAGTGGAGAAACACAGGTAGGACTCAAACTCCAACAATTTACATTCACTAATGACTTTTATGTATTGCATGTGACAGGTTGTCAAATAGTACTTGGAGCTGGTTGGTTAAGGTCCCTGGGGGACATTTTATGGAATTTTGAAACCATGAGGATGAAATTCATACTTAAAGGCACGGAGTATCAGTTACAGGGAGAAACTGAACATGAGGCCACTGTGATCAATTGTTATTCTATGACCAAACTTCTCAGAAAAGAGAGAGAGGCTATGATTGTGGAGGTATCTCCTGCTCAAATGAATGAAAAGGTGGCACTACAGGATCCCCAAATACAGGCTCTCTTGGATAAATATGCAGATCTTTTTGTAGCTCCTACTTCTTCACCCCCATCCAGATCACAGGATCACAAGATTGAACTGCTACCTAACACACCACCAATTAGTAAAACTGAAATAGAGAAAATTGTCCAAGAACTGCTTGCAAATGGAGTTATTAGACCAAGTGTTAGTCCATTTTCCTCACCAGTCTTGTTGGTAAAAAAGAAAGATGGCACATGGAGAATGTGTGTGGATTACAGATCTTTAAATGCTGCCACAGTGAAAGATAAGCACCCCATACCAGTTGTTGATGAACTTTTGGATGAAGTTCATGGGTCTACCATTTTCTCTAAGCTTGATCTACGATCTAGATATCACCAGATTATGATGCATGCTGATGATGTGAGTAAAACTTCATTCAGAACACACAATGGACACTACGTGTTTATCAGATTGCAGCAACACGCTCTGAAGGTCAAGCAGAGTAAATGTAGCTTTGGAGTGAAATCAGTGGAGTACCTTGGCCATGTTATTAGAGCAGAAGGAGTATCAGTTGACCCTGCAAAGATTGAATGTATCAAGAATTGGAGCAAGCCACAGTCTGTCAAGGGCCTTAGAGGTTTTTTGGGTCTTGCTGGTTATTATAGAAAATATGTCAAACACTTTGGCACCATAGCCAAACCACTCACTGACATGTTGAAAACTGGGGGGTTTCATTGGACATCAGAATCAGAAAGAGCCTTTGAGACTCTCAAACATGCTCTCATGCATACTCCGGTGTTAGCCTTGCCCGATTTTTTAAAGAAATTTGTCATTGAATGTGACGCTTCAGGAGTGGGAATTGGAGCTGTTTTGTCTCAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1015

Amino Acids

113.02

Weight (kDa)

8.27

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVP_2 PF08284 580 - 661 2.7e-08 Retroviral aspartyl protease
RVT_1 PF00078 800 - 870 1.2e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 967 - 1015 1e-14 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 2849
AccI GTMKAC 1 cut(s) 2519
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 316, 456
AclWI GGATC 9 cut(s) 136, 316, 983, 1179, 1329, 1496, 2180, 2193, 2274
AcoI YGGCCR 4 cut(s) 3, 390, 1684, 2704
AcsI RAATTY 8 cut(s) 934, 965, 1166, 1382, 1508, 2002, 2024, 2987
AcuI CTGAAG 5 cut(s) 1281, 1617, 1878, 2672, 2994
AdeI CACNNNGTG 1 cut(s) 2623
AfaI GTAC 8 cut(s) 253, 1106, 1575, 1629, 1815, 1863, 1959, 2699
AfiI CCNNNNNNNGG 6 cut(s) 115, 202, 678, 1209, 1662, 2197
AflIII ACRYGT 3 cut(s) 1107, 2622, 2874
AhdI GACNNNNNGTC 2 cut(s) 588, 1947
AhlI ACTAGT 2 cut(s) 122, 1847
AjnI CCWGG 5 cut(s) 87, 552, 1246, 1361, 1983
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AleI CACNNNNGTG 1 cut(s) 530
Alw21I GWGCWC 5 cut(s) 79, 345, 654, 775, 1375
Alw26I GTCTC 4 cut(s) 42, 236, 2921, 3042
Alw44I GTGCAC 1 cut(s) 75
AlwI GGATC 9 cut(s) 136, 316, 983, 1179, 1329, 1496, 2180, 2193, 2274
Ama87I CYCGRG 2 cut(s) 586, 647
ApaLI GTGCAC 1 cut(s) 75
ApeKI GCWGC 9 cut(s) 100, 170, 217, 220, 510, 1199, 1789, 2453, 2638
ApoI RAATTY 8 cut(s) 934, 965, 1166, 1382, 1508, 2002, 2024, 2987
Asp700I GAANNNNTTC 2 cut(s) 476, 1057
AspS9I GGNCC 5 cut(s) 313, 680, 1528, 1980, 2791
AsuC2I CCSGG 1 cut(s) 1527
AsuHPI GGTGA 9 cut(s) 77, 217, 286, 370, 542, 1387, 2238, 2376, 2549
AsuII TTCGAA 1 cut(s) 1512
AvaI CYCGRG 2 cut(s) 586, 647
AvaII GGWCC 2 cut(s) 313, 1980
BaeGI GKGCMC 2 cut(s) 79, 1802
BalI TGGCCA 4 cut(s) 5, 392, 1686, 2706
BamHI GGATCC 1 cut(s) 2185
BanI GGYRCC 1 cut(s) 2849
BanII GRGCYC 2 cut(s) 1246, 1656
BbsI GAAGAC 1 cut(s) 1528
Bbv12I GWGCWC 5 cut(s) 79, 345, 654, 775, 1375
BbvI GCAGC 9 cut(s) 87, 157, 204, 232, 522, 1186, 1776, 2440, 2650
BccI CCATC 9 cut(s) 117, 299, 340, 443, 718, 891, 1095, 2260, 2405
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 5 cut(s) 89, 554, 1248, 1363, 1985
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 3 cut(s) 699, 1015, 2085
BcnI CCSGG 1 cut(s) 1527
BcoDI GTCTC 4 cut(s) 42, 236, 2921, 3042
BcuI ACTAGT 2 cut(s) 122, 1847
BfmI CTRYAG 5 cut(s) 218, 910, 1537, 1667, 2180
BfuI GTATCC 1 cut(s) 427
BglII AGATCT 3 cut(s) 395, 2221, 2441
BisI GCNGC 9 cut(s) 101, 171, 218, 221, 511, 1200, 1790, 2454, 2639
BlsI GCNGC 9 cut(s) 102, 172, 219, 222, 512, 1201, 1791, 2455, 2640
BmcAI AGTACT 1 cut(s) 1959
Bme1390I CCNGG 6 cut(s) 89, 554, 1248, 1363, 1527, 1985
Bme18I GGWCC 2 cut(s) 313, 1980
BmeRI GACNNNNNGTC 2 cut(s) 588, 1947
BmeT110I CYCGRG 2 cut(s) 586, 647
BmgT120I GGNCC 5 cut(s) 313, 680, 1528, 1980, 2791
BmiI GGNNCC 7 cut(s) 325, 454, 540, 1245, 1982, 2187, 2851
BmrFI CCNGG 6 cut(s) 89, 554, 1248, 1363, 1527, 1985
BmrI ACTGGG 2 cut(s) 817, 2895
BmsI GCATC 3 cut(s) 783, 1492, 2559
BmuI ACTGGG 2 cut(s) 817, 2895
BpiI GAAGAC 1 cut(s) 1528
BpmI CTGGAG 3 cut(s) 1230, 1488, 1779
Bpu14I TTCGAA 1 cut(s) 1512
BpuEI CTTGAG 2 cut(s) 1778, 3024
BpuMI CCSGG 1 cut(s) 1527
BsaAI YACGTR 1 cut(s) 2623
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 6 cut(s) 553, 1203, 1362, 1983, 1984, 2701
BsaWI WCCGGW 3 cut(s) 301, 337, 2956
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 202, 678, 1209, 1662, 2197
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 5 cut(s) 823, 1228, 2387, 2483, 2890
Bse3DI GCAATG 1 cut(s) 1062
BseBI CCWGG 5 cut(s) 89, 554, 1248, 1363, 1985
BseDI CCNNGG 6 cut(s) 553, 1203, 1362, 1983, 1984, 2701
BseLI CCNNNNNNNGG 6 cut(s) 115, 202, 678, 1209, 1662, 2197
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 3 cut(s) 390, 557, 2128
BseNI ACTGG 5 cut(s) 823, 1228, 2387, 2483, 2890
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 2 cut(s) 79, 1802
BseXI GCAGC 9 cut(s) 87, 157, 204, 232, 522, 1186, 1776, 2440, 2650
BseYI CCCAGC 1 cut(s) 1785
Bsh1236I CGCG 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 2849
BsiHKAI GWGCWC 5 cut(s) 79, 345, 654, 775, 1375
BsiHKCI CYCGRG 2 cut(s) 586, 647
BsiSI CCGG 5 cut(s) 246, 302, 338, 1526, 2957
BslFI GGGAC 2 cut(s) 1966, 2003
BslI CCNNNNNNNGG 6 cut(s) 115, 202, 678, 1209, 1662, 2197
BsmAI GTCTC 4 cut(s) 42, 236, 2921, 3042
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 1966, 2003
BsmI GAATGC 1 cut(s) 478
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 2 cut(s) 586, 647
Bsp119I TTCGAA 1 cut(s) 1512
Bsp1286I GDGCHC 8 cut(s) 79, 345, 654, 775, 1246, 1375, 1656, 1802
BspACI CCGC 3 cut(s) 44, 316, 456
BspCNI CTCAG 3 cut(s) 391, 556, 2127
BspFNI CGCG 1 cut(s) 46
BspHI TCATGA 2 cut(s) 666, 1557
BspLI GGNNCC 7 cut(s) 325, 454, 540, 1245, 1982, 2187, 2851
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 9 cut(s) 136, 316, 983, 1179, 1329, 1496, 2180, 2193, 2274
BspT104I TTCGAA 1 cut(s) 1512
BspT107I GGYRCC 1 cut(s) 2849
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 5 cut(s) 823, 1228, 2387, 2483, 2890
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 6 cut(s) 553, 1203, 1362, 1983, 1984, 2701
BssT1I CCWWGG 2 cut(s) 1203, 2701
Bst2UI CCWGG 5 cut(s) 89, 554, 1248, 1363, 1985
Bst4CI ACNGT 9 cut(s) 388, 415, 597, 790, 1272, 1538, 2083, 2461, 2781
Bst6I CTCTTC 2 cut(s) 382, 1293
BstBAI YACGTR 1 cut(s) 2623
BstBI TTCGAA 1 cut(s) 1512
BstC8I GCNNGC 5 cut(s) 515, 1553, 2343, 2574, 2774
BstDEI CTNAG 8 cut(s) 273, 399, 543, 688, 765, 2114, 2532, 2795
BstENI CCTNNNNNAGG 2 cut(s) 1207, 1660
BstFNI CGCG 1 cut(s) 46
BstMAI GTCTC 4 cut(s) 42, 236, 2921, 3042
BstMWI GCNNNNNNNGC 2 cut(s) 179, 1205
BstNI CCWGG 5 cut(s) 89, 554, 1248, 1363, 1985
BstNSI RCATGY 6 cut(s) 1111, 1353, 1940, 2576, 2878, 2942
BstSCI CCNGG 6 cut(s) 87, 552, 1246, 1361, 1525, 1983
BstSFI CTRYAG 5 cut(s) 218, 910, 1537, 1667, 2180
BstSLI GKGCMC 2 cut(s) 79, 1802
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 9 cut(s) 87, 157, 204, 232, 522, 1186, 1776, 2440, 2650
BstV2I GAAGAC 1 cut(s) 1528
BstX2I RGATCY 8 cut(s) 141, 395, 988, 1184, 1334, 2185, 2221, 2441
BstYI RGATCY 8 cut(s) 141, 395, 988, 1184, 1334, 2185, 2221, 2441
BsuI GTATCC 1 cut(s) 427
BtsIMutI CAGTG 8 cut(s) 786, 976, 1177, 1236, 2079, 2466, 2697, 2868
Cac8I GCNNGC 5 cut(s) 515, 1553, 2343, 2574, 2774
CciI TCATGA 2 cut(s) 666, 1557
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 5 cut(s) 313, 680, 1528, 1980, 2791
CpoI CGGWCCG 1 cut(s) 313
CseI GACGC 2 cut(s) 35, 3014
Csp6I GTAC 8 cut(s) 252, 1105, 1574, 1628, 1814, 1862, 1958, 2698
CspI CGGWCCG 1 cut(s) 313
CviQI GTAC 8 cut(s) 252, 1105, 1574, 1628, 1814, 1862, 1958, 2698
DdeI CTNAG 8 cut(s) 273, 399, 543, 688, 765, 2114, 2532, 2795
DraI TTTAAA 3 cut(s) 496, 2448, 2982
DraIII CACNNNGTG 1 cut(s) 2623
DrdI GACNNNNNNGTC 1 cut(s) 311
DriI GACNNNNNGTC 2 cut(s) 588, 1947
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 4 cut(s) 3, 390, 1684, 2704
Eam1104I CTCTTC 2 cut(s) 382, 1293
Eam1105I GACNNNNNGTC 2 cut(s) 588, 1947
EarI CTCTTC 2 cut(s) 382, 1293
Eco130I CCWWGG 2 cut(s) 1203, 2701
Eco24I GRGCYC 2 cut(s) 1246, 1656
Eco31I GGTCTC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 2555
Eco47I GGWCC 2 cut(s) 313, 1980
Eco57I CTGAAG 5 cut(s) 1281, 1617, 1878, 2672, 2994
Eco88I CYCGRG 2 cut(s) 586, 647
EcoNI CCTNNNNNAGG 2 cut(s) 1207, 1660
EcoO109I RGGNCCY 2 cut(s) 1980, 2791
EcoRI GAATTC 3 cut(s) 965, 1166, 1508
EcoRII CCWGG 5 cut(s) 87, 552, 1246, 1361, 1983
EcoRV GATATC 1 cut(s) 2555
EcoT14I CCWWGG 2 cut(s) 1203, 2701
EcoT22I ATGCAT 3 cut(s) 1077, 2574, 2952
EcoT38I GRGCYC 2 cut(s) 1246, 1656
ErhI CCWWGG 2 cut(s) 1203, 2701
Esp3I CGTCTC 1 cut(s) 42
FalI AAGNNNNNCTT 2 cut(s) 2325, 2357
FaqI GGGAC 2 cut(s) 1966, 2003
FbaI TGATCA 3 cut(s) 699, 1015, 2085
FblI GTMKAC 1 cut(s) 2519
Fnu4HI GCNGC 9 cut(s) 101, 171, 218, 221, 511, 1200, 1790, 2454, 2639
FriOI GRGCYC 2 cut(s) 1246, 1656
Fsp4HI GCNGC 9 cut(s) 101, 171, 218, 221, 511, 1200, 1790, 2454, 2639
GluI GCNGC 9 cut(s) 101, 171, 218, 221, 511, 1200, 1790, 2454, 2639
GsaI CCCAGC 1 cut(s) 1789
GsuI CTGGAG 3 cut(s) 1230, 1488, 1779
HapII CCGG 5 cut(s) 246, 302, 338, 1526, 2957
HgaI GACGC 2 cut(s) 35, 3014
HincII GTYRAC 1 cut(s) 2737
HindII GTYRAC 1 cut(s) 2737
HindIII AAGCTT 5 cut(s) 147, 1055, 1089, 1820, 2534
HinfI GANTC 7 cut(s) 371, 589, 1251, 1754, 1890, 2909, 2929
HpaII CCGG 5 cut(s) 246, 302, 338, 1526, 2957
HphI GGTGA 9 cut(s) 77, 217, 286, 370, 542, 1387, 2238, 2376, 2549
Hpy166II GTNNAC 5 cut(s) 77, 593, 1816, 2520, 2737
Hpy8I GTNNAC 5 cut(s) 77, 593, 1816, 2520, 2737
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 6 cut(s) 60, 187, 241, 1288, 2647, 2717
HpyCH4III ACNGT 9 cut(s) 388, 415, 597, 790, 1272, 1538, 2083, 2461, 2781
HpyCH4IV ACGT 3 cut(s) 66, 285, 2622
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 1205
HpyF3I CTNAG 8 cut(s) 273, 399, 543, 688, 765, 2114, 2532, 2795
HpySE526I ACGT 3 cut(s) 66, 285, 2622
Ksp22I TGATCA 3 cut(s) 699, 1015, 2085
Lsp1109I GCAGC 9 cut(s) 87, 157, 204, 232, 522, 1186, 1776, 2440, 2650
LweI GCATC 3 cut(s) 783, 1492, 2559
MaeII ACGT 3 cut(s) 66, 285, 2622
MaeIII GTNAC 7 cut(s) 523, 564, 628, 1443, 1939, 2052, 3002
MboII GAAGA 4 cut(s) 369, 1310, 1533, 2235
MfeI CAATTG 1 cut(s) 2089
MflI RGATCY 8 cut(s) 141, 395, 988, 1184, 1334, 2185, 2221, 2441
MhlI GDGCHC 8 cut(s) 79, 345, 654, 775, 1246, 1375, 1656, 1802
MlsI TGGCCA 4 cut(s) 5, 392, 1686, 2706
MluNI TGGCCA 4 cut(s) 5, 392, 1686, 2706
MlyI GAGTC 4 cut(s) 380, 598, 1884, 2923
MmeI TCCRAC 3 cut(s) 370, 1348, 1924
Mox20I TGGCCA 4 cut(s) 5, 392, 1686, 2706
Mph1103I ATGCAT 3 cut(s) 1077, 2574, 2952
MroXI GAANNNNTTC 2 cut(s) 476, 1057
MscI TGGCCA 4 cut(s) 5, 392, 1686, 2706
MslI CAYNNNNRTG 7 cut(s) 530, 791, 1562, 1584, 2018, 2577, 2873
Msp20I TGGCCA 4 cut(s) 5, 392, 1686, 2706
MspA1I CMGCKG 1 cut(s) 1789
MspI CCGG 5 cut(s) 246, 302, 338, 1526, 2957
MspR9I CCNGG 6 cut(s) 89, 554, 1248, 1363, 1527, 1985
MunI CAATTG 1 cut(s) 2089
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 5 cut(s) 89, 554, 1248, 1363, 1985
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 179, 1205
NciI CCSGG 1 cut(s) 1527
NlaIV GGNNCC 7 cut(s) 325, 454, 540, 1245, 1982, 2187, 2851
NmuCI GTSAC 5 cut(s) 523, 564, 1443, 1939, 3002
NsiI ATGCAT 3 cut(s) 1077, 2574, 2952
NspI RCATGY 6 cut(s) 1111, 1353, 1940, 2576, 2878, 2942
NspV TTCGAA 1 cut(s) 1512
OliI CACNNNNGTG 1 cut(s) 530
PaeI GCATGC 1 cut(s) 2576
PaeR7I CTCGAG 1 cut(s) 586
PagI TCATGA 2 cut(s) 666, 1557
PasI CCCWGGG 1 cut(s) 1984
PciI ACATGT 2 cut(s) 1107, 2874
PctI GAATGC 1 cut(s) 478
PdmI GAANNNNTTC 2 cut(s) 476, 1057
PfeI GAWTC 3 cut(s) 1251, 1754, 2909
PfoI TCCNGGA 1 cut(s) 1246
PkrI GCNGC 9 cut(s) 102, 172, 219, 222, 512, 1201, 1791, 2455, 2640
PleI GAGTC 4 cut(s) 379, 597, 1884, 2923
PpsI GAGTC 4 cut(s) 379, 597, 1884, 2923
Ppu21I YACGTR 1 cut(s) 2623
PpuMI RGGWCCY 1 cut(s) 1980
PscI ACATGT 2 cut(s) 1107, 2874
Psp5II RGGWCCY 1 cut(s) 1980
Psp6I CCWGG 5 cut(s) 87, 552, 1246, 1361, 1983
PspFI CCCAGC 1 cut(s) 1785
PspGI CCWGG 5 cut(s) 87, 552, 1246, 1361, 1983
PspN4I GGNNCC 7 cut(s) 325, 454, 540, 1245, 1982, 2187, 2851
PspPI GGNCC 5 cut(s) 313, 680, 1528, 1980, 2791
PspPPI RGGWCCY 1 cut(s) 1980
PspXI VCTCGAGB 1 cut(s) 586
PstI CTGCAG 1 cut(s) 222
PsuI RGATCY 8 cut(s) 141, 395, 988, 1184, 1334, 2185, 2221, 2441
PvuII CAGCTG 1 cut(s) 1789
RsaI GTAC 8 cut(s) 253, 1106, 1575, 1629, 1815, 1863, 1959, 2699
RsaNI GTAC 8 cut(s) 252, 1105, 1574, 1628, 1814, 1862, 1958, 2698
RseI CAYNNNNRTG 7 cut(s) 530, 791, 1562, 1584, 2018, 2577, 2873
Rsr2I CGGWCCG 1 cut(s) 313
RsrII CGGWCCG 1 cut(s) 313
SatI GCNGC 9 cut(s) 101, 171, 218, 221, 511, 1200, 1790, 2454, 2639
Sau96I GGNCC 5 cut(s) 313, 680, 1528, 1980, 2791
ScaI AGTACT 1 cut(s) 1959
SchI GAGTC 4 cut(s) 380, 598, 1884, 2923
ScrFI CCNGG 6 cut(s) 89, 554, 1248, 1363, 1527, 1985
SduI GDGCHC 8 cut(s) 79, 345, 654, 775, 1246, 1375, 1656, 1802
SfaNI GCATC 3 cut(s) 783, 1492, 2559
SfcI CTRYAG 5 cut(s) 218, 910, 1537, 1667, 2180
Sfr274I CTCGAG 1 cut(s) 586
SfuI TTCGAA 1 cut(s) 1512
SinI GGWCC 2 cut(s) 313, 1980
SlaI CTCGAG 1 cut(s) 586
SmiMI CAYNNNNRTG 7 cut(s) 530, 791, 1562, 1584, 2018, 2577, 2873
SmlI CTYRAG 4 cut(s) 11, 586, 1793, 3039
SmoI CTYRAG 4 cut(s) 11, 586, 1793, 3039
SpeI ACTAGT 2 cut(s) 122, 1847
SphI GCATGC 1 cut(s) 2576
SsiI CCGC 3 cut(s) 44, 316, 456
SspI AATATT 2 cut(s) 1022, 1842
StyD4I CCNGG 6 cut(s) 87, 552, 1246, 1361, 1525, 1983
StyI CCWWGG 2 cut(s) 1203, 2701
TaaI ACNGT 9 cut(s) 388, 415, 597, 790, 1272, 1538, 2083, 2461, 2781
TaiI ACGT 3 cut(s) 69, 288, 2625
TaqI TCGA 4 cut(s) 463, 587, 1512, 1519
TaqII GACCGA 1 cut(s) 301
TatI WGTACW 3 cut(s) 1813, 1861, 1957
TfiI GAWTC 3 cut(s) 1251, 1754, 2909
TscAI CASTG 8 cut(s) 793, 976, 1177, 1236, 2086, 2466, 2697, 2875
TseFI GTSAC 5 cut(s) 523, 564, 1443, 1939, 3002
TseI GCWGC 9 cut(s) 100, 170, 217, 220, 510, 1199, 1789, 2453, 2638
Tsp45I GTSAC 5 cut(s) 523, 564, 1443, 1939, 3002
TspGWI ACGGA 4 cut(s) 321, 383, 470, 2057
TspRI CASTG 8 cut(s) 793, 976, 1177, 1236, 2086, 2466, 2697, 2875
VneI GTGCAC 1 cut(s) 75
VpaK11BI GGWCC 2 cut(s) 313, 1980
XagI CCTNNNNNAGG 2 cut(s) 1207, 1660
XapI RAATTY 8 cut(s) 934, 965, 1166, 1382, 1508, 2002, 2024, 2987
XbaI TCTAGA 1 cut(s) 2549
XceI RCATGY 6 cut(s) 1111, 1353, 1940, 2576, 2878, 2942
XhoI CTCGAG 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 2519
XmnI GAANNNNTTC 2 cut(s) 476, 1057
ZrmI AGTACT 1 cut(s) 1959
Zsp2I ATGCAT 3 cut(s) 1077, 2574, 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.