Rroxscaffold_7G00197340

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000756
Physical Location & Seq
Forward (+)
41520986 .. 41528441
7456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197340.1

Sequence Viewer

Length: 537 bp
ATGGGAGATGGGCATAACTCCCCTGCTACTATGCAACTCAAGGGGATGTTTAACAACAAAGGTGTGCATGTGCTTATTGACTCGGGGGCCACACATAACTTCATACATCCTCGGCTGCTCAAGGGTACCAAAACTCAAGTGCACAAGCTACCTCCATTGAATGTGGTTTTGGCCGGTGGTGCTAAGATGAAAACTCAAGGTGAGGTACAGGTAAATTTGCAGTTACAAGATTTTCAATTTTTAGCCGACTACTATGTGTTGCCTGTGACTGGTTGTGAGGTTGTGTTGGGGGCCAGCTGGTTAAGGTCTCTTGGTGACATTTTATGGAACTTTGAAACAATGAGGATGAAATTCACGGTACAAGGTTCCGAGTTCCAGCTGCAAGGTGAAAGCGAGGCTAAAGCTATGGTCATTAGTTGCAAAGCTATGACTCGGCTACTTAGAAAGGAAAGAGAAGCTATGCTAGTACGGATGCAGCCAACTTTCCCAAGTCAAACAAGTAACCCCACGGGACCCAAAACTGCAAGCCGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

178

Amino Acids

19.75

Weight (kDa)

9.51

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVP_2 PF08284 11 - 103 2.2e-09 Retroviral aspartyl protease
gag-asp_proteas PF13975 14 - 102 7.7e-09 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 14 - 99 4.2e-06 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 2 cut(s) 214, 350
AfaI GTAC 4 cut(s) 127, 207, 360, 468
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 3 cut(s) 160, 236, 335
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 6 cut(s) 148, 297, 379, 404, 425, 458
AluI AGCT 6 cut(s) 148, 297, 379, 404, 425, 458
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 312
Alw44I GTGCAC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 3 cut(s) 87, 171, 291
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 3 cut(s) 115, 379, 475
ApoI RAATTY 2 cut(s) 214, 350
Asp718I GGTACC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 87, 291, 512
AsuHPI GGTGA 3 cut(s) 212, 326, 398
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 102, 366, 487
BccI CCATC 1 cut(s) 2
BceAI ACGGC 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 464
BisI GCNGC 3 cut(s) 116, 380, 476
BlsI GCNGC 3 cut(s) 117, 381, 477
Bme18I GGWCC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 3 cut(s) 87, 291, 512
BmiI GGNNCC 6 cut(s) 88, 127, 292, 367, 513, 514
BmsI GCATC 1 cut(s) 462
BpuEI CTTGAG 4 cut(s) 23, 104, 120, 180
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 110, 507
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 110, 507
BseGI GGATG 4 cut(s) 51, 106, 351, 477
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 274
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 3 cut(s) 102, 366, 487
BshFI GGCC 3 cut(s) 89, 173, 293
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 3 cut(s) 89, 173, 293
Bso31I GGTCTC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 144
BspANI GGCC 3 cut(s) 89, 173, 293
BspLI GGNNCC 6 cut(s) 88, 127, 292, 367, 513, 514
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 110, 507
Bst4CI ACNGT 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 295, 526
BstDEI CTNAG 2 cut(s) 183, 440
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 4 cut(s) 51, 106, 351, 477
BstMAI GTCTC 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 102, 366, 487
BsuRI GGCC 3 cut(s) 89, 173, 293
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 4 cut(s) 51, 106, 351, 477
Cac8I GCNNGC 2 cut(s) 295, 526
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 3 cut(s) 87, 291, 512
Csp6I GTAC 4 cut(s) 126, 206, 359, 467
CviAII CATG 1 cut(s) 68
CviQI GTAC 4 cut(s) 126, 206, 359, 467
DdeI CTNAG 2 cut(s) 183, 440
EaeI YGGCCR 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 512
FaeI CATG 1 cut(s) 71
FaqI GGGAC 1 cut(s) 525
FatI CATG 1 cut(s) 67
Fnu4HI GCNGC 3 cut(s) 116, 380, 476
FokI GGATG 4 cut(s) 58, 93, 358, 484
Fsp4HI GCNGC 3 cut(s) 116, 380, 476
FspBI CTAG 1 cut(s) 464
GluI GCNGC 3 cut(s) 116, 380, 476
HaeIII GGCC 3 cut(s) 89, 173, 293
HapII CCGG 1 cut(s) 174
Hin1II CATG 1 cut(s) 71
HinfI GANTC 2 cut(s) 80, 430
HpaII CCGG 1 cut(s) 174
HphI GGTGA 3 cut(s) 212, 326, 398
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 8 cut(s) 34, 67, 142, 220, 382, 420, 475, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 183, 440
Hsp92II CATG 1 cut(s) 71
KflI GGGWCCC 1 cut(s) 512
KpnI GGTACC 1 cut(s) 129
LpnPI CCDG 8 cut(s) 36, 187, 194, 255, 276, 283, 307, 389
Lsp1109I GCAGC 3 cut(s) 102, 366, 487
LweI GCATC 1 cut(s) 462
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 4 cut(s) 222, 265, 314, 500
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 214, 236, 350
MlyI GAGTC 2 cut(s) 74, 424
MnlI CCTC 6 cut(s) 120, 162, 196, 271, 336, 388
MseI TTAA 3 cut(s) 51, 302, 535
MspA1I CMGCKG 2 cut(s) 297, 379
MspI CCGG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 179
NlaIII CATG 1 cut(s) 71
NlaIV GGNNCC 6 cut(s) 88, 127, 292, 367, 513, 514
NmeAIII GCCGAG 2 cut(s) 91, 412
NmuCI GTSAC 2 cut(s) 265, 314
NspI RCATGY 1 cut(s) 71
PkrI GCNGC 3 cut(s) 117, 381, 477
PleI GAGTC 2 cut(s) 74, 424
PpsI GAGTC 2 cut(s) 74, 424
PpuMI RGGWCCY 1 cut(s) 512
Psp5II RGGWCCY 1 cut(s) 512
PspN4I GGNNCC 6 cut(s) 88, 127, 292, 367, 513, 514
PspPI GGNCC 3 cut(s) 87, 291, 512
PspPPI RGGWCCY 1 cut(s) 512
PvuII CAGCTG 2 cut(s) 297, 379
RsaI GTAC 4 cut(s) 127, 207, 360, 468
RsaNI GTAC 4 cut(s) 126, 206, 359, 467
SaqAI TTAA 3 cut(s) 51, 302, 535
SatI GCNGC 3 cut(s) 116, 380, 476
Sau96I GGNCC 3 cut(s) 87, 291, 512
SchI GAGTC 2 cut(s) 74, 424
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 1 cut(s) 462
SinI GGWCC 1 cut(s) 512
SmlI CTYRAG 4 cut(s) 38, 119, 135, 195
SmoI CTYRAG 4 cut(s) 38, 119, 135, 195
Sse9I AATT 3 cut(s) 214, 236, 350
SspMI CTAG 1 cut(s) 464
TaaI ACNGT 1 cut(s) 358
TasI AATT 3 cut(s) 214, 236, 350
Tru1I TTAA 3 cut(s) 51, 302, 535
Tru9I TTAA 3 cut(s) 51, 302, 535
TseFI GTSAC 2 cut(s) 265, 314
TseI GCWGC 3 cut(s) 115, 379, 475
Tsp45I GTSAC 2 cut(s) 265, 314
TspDTI ATGAA 3 cut(s) 91, 203, 362
TspGWI ACGGA 1 cut(s) 484
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 512
XapI RAATTY 2 cut(s) 214, 350
XceI RCATGY 1 cut(s) 71
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.