RchiOBHm_Chr5g0075951

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000756
Physical Location & Seq
Reverse (-)
81656009 .. 81657228
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35065

Sequence Viewer

Length: 900 bp
ATGGGATCAGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGACACTGTAACATTATATGCACACAACCATGGAGAATAGCGGCTATTTCTGTGGCCAACAGAGTCGCTGATGAGCGTTGTGAACCTTCACCTGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTGCGCCTTGATAATGCAAGTAATGAAAAGACTAAGCTTCTGTTTTGCACGACAGATATTCTTCTGAGAAAATTTGTGGGAGACAGAAACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACACGAGCGGTCTCTTTTGGGTGATTTCCTACTCATAGTTTTGAAGAATCTGATTGAGAAGCAATCAGCTCTTAACACACCAAAACTGAAAGTTATTCTAATGTCTGCAACTGTTGATTCCAATTTGTTTTCAAATTATTTTGGTGGTTGTCCTGTGATTACTGCAGAAGGTAGAACACATCCAGTGACAACTTCTTATGATAAAAAGCACACTGAAAGAGAATTTGGAGTAGGAGATTGGGTGTACTTGAAGCTGCAGCCATATAAGCAGCATTCTATGCACAAAGGTGAGTTTCACAAATTGTCCCAGAGGTACTATGGGCCCTTCCAAGTGGTGGAAAGAGTGGGAAAAGTGGCTTACAGATTGCAGCTGCCAGTTACTGCAAAAATTCATGATGTGTTTCATGTCTCTCTGCTCAAAAAGAAATTGGGGGACCTCATCTCTGCACAACCTCAGCTACCCCATATTATTGATCCAGAGAATCCAAAGTGGGAACCCTTTGCTGTGCTTGATAGGAGGGTGCTCATGAAAAGAGGAGCTGTTATTAACCAATGGTTAGTGCAGTGTAGGCCTGACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

299

Amino Acids

33.74

Weight (kDa)

9.23

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 2 - 147 7.3e-07 DEAD/DEAH box helicase
SH3_Tf2-1 PF24626 184 - 247 4e-23 Tf2-1-like, SH3 domain
Integrase_p58_C PF22938 213 - 247 7.4e-08 Integrase p58, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 325
AciI CCGC 2 cut(s) 110, 325
AclWI GGATC 3 cut(s) 13, 46, 785
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 3 cut(s) 263, 539, 705
AfaI GTAC 3 cut(s) 318, 563, 632
AfiI CCNNNNNNNGG 2 cut(s) 161, 652
AgsI TTSAA 4 cut(s) 168, 361, 450, 568
AjiI CACGTC 1 cut(s) 65
AjnI CCWGG 1 cut(s) 160
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 6 cut(s) 229, 386, 571, 688, 775, 857
AluI AGCT 6 cut(s) 229, 386, 571, 688, 775, 857
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 3 cut(s) 267, 333, 730
AlwI GGATC 3 cut(s) 13, 46, 785
AlwNI CAGNNNCTG 1 cut(s) 698
AoxI GGCC 3 cut(s) 123, 638, 887
ApaI GGGCCC 1 cut(s) 642
ApeKI GCWGC 5 cut(s) 571, 574, 586, 685, 688
ApoI RAATTY 3 cut(s) 263, 539, 705
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 3 cut(s) 638, 639, 751
AsuHPI GGTGA 3 cut(s) 150, 350, 617
AvaII GGWCC 1 cut(s) 751
BaeGI GKGCMC 1 cut(s) 642
BalI TGGCCA 1 cut(s) 125
BanII GRGCYC 1 cut(s) 642
BauI CACGAG 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 843
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 5 cut(s) 558, 586, 598, 675, 697
BciT130I CCWGG 1 cut(s) 162
BcoDI GTCTC 3 cut(s) 267, 333, 730
BfmI CTRYAG 2 cut(s) 480, 572
BisI GCNGC 6 cut(s) 111, 572, 575, 587, 686, 689
BlsI GCNGC 6 cut(s) 112, 573, 576, 588, 687, 690
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 751
BmgBI CACGTC 1 cut(s) 65
BmgT120I GGNCC 3 cut(s) 638, 639, 751
BmiI GGNNCC 4 cut(s) 27, 640, 752, 813
BmrFI CCNGG 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 652
Bse1I ACTGG 4 cut(s) 55, 292, 500, 692
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 2 cut(s) 49, 496
BseLI CCNNNNNNNGG 2 cut(s) 161, 652
BseMII CTCAG 3 cut(s) 35, 248, 785
BseNI ACTGG 4 cut(s) 55, 292, 500, 692
BseRI GAGGAG 1 cut(s) 867
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 5 cut(s) 558, 586, 598, 675, 697
BsgI GTGCAG 2 cut(s) 747, 899
BshFI GGCC 3 cut(s) 125, 640, 889
BsiHKAI GWGCWC 1 cut(s) 843
BslFI GGGAC 2 cut(s) 607, 764
BslI CCNNNNNNNGG 2 cut(s) 161, 652
BsmAI GTCTC 3 cut(s) 267, 333, 730
BsmFI GGGAC 2 cut(s) 607, 764
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 3 cut(s) 125, 640, 889
Bso31I GGTCTC 1 cut(s) 333
Bsp120I GGGCCC 1 cut(s) 638
Bsp1286I GDGCHC 2 cut(s) 642, 843
Bsp143I GATC 3 cut(s) 5, 51, 790
Bsp19I CCATGG 1 cut(s) 98
BspACI CCGC 2 cut(s) 110, 325
BspANI GGCC 3 cut(s) 125, 640, 889
BspCNI CTCAG 3 cut(s) 34, 249, 784
BspHI TCATGA 2 cut(s) 709, 843
BspLI GGNNCC 4 cut(s) 27, 640, 752, 813
BspMAI CTGCAG 2 cut(s) 484, 576
BspPI GGATC 3 cut(s) 13, 46, 785
BspTNI GGTCTC 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 325
BsrI ACTGG 4 cut(s) 55, 292, 500, 692
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 3 cut(s) 5, 51, 790
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 77, 430
BstAPI GCANNNNNTGC 1 cut(s) 595
BstDEI CTNAG 4 cut(s) 21, 225, 257, 771
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 49, 496
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 8, 54, 793
BstMAI GTCTC 3 cut(s) 267, 333, 730
BstMBI GATC 3 cut(s) 5, 51, 790
BstMWI GCNNNNNNNGC 3 cut(s) 583, 595, 886
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 2 cut(s) 480, 572
BstSLI GKGCMC 1 cut(s) 642
BstV1I GCAGC 5 cut(s) 558, 586, 598, 675, 697
BsuRI GGCC 3 cut(s) 125, 640, 889
BtgI CCRYGG 1 cut(s) 98
BtrI CACGTC 1 cut(s) 65
BtsCI GGATG 2 cut(s) 49, 496
BtsI GCAGTG 1 cut(s) 887
BtsIMutI CAGTG 4 cut(s) 73, 507, 528, 887
CaiI CAGNNNCTG 1 cut(s) 698
CciI TCATGA 2 cut(s) 709, 843
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 3 cut(s) 638, 639, 751
CsiI ACCWGGT 1 cut(s) 160
Csp6I GTAC 3 cut(s) 317, 562, 631
CviAII CATG 6 cut(s) 49, 99, 299, 710, 722, 844
CviQI GTAC 3 cut(s) 317, 562, 631
DdeI CTNAG 4 cut(s) 21, 225, 257, 771
DpnI GATC 3 cut(s) 7, 53, 792
DpnII GATC 3 cut(s) 5, 51, 790
EaeI YGGCCR 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 98
Eco147I AGGCCT 1 cut(s) 889
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 751
EcoO109I RGGNCCY 2 cut(s) 639, 751
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 6 cut(s) 52, 102, 302, 713, 725, 847
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FaqI GGGAC 2 cut(s) 607, 764
FatI CATG 6 cut(s) 48, 98, 298, 709, 721, 843
Fnu4HI GCNGC 6 cut(s) 111, 572, 575, 587, 686, 689
FokI GGATG 2 cut(s) 56, 483
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 6 cut(s) 111, 572, 575, 587, 686, 689
GlaI GCGC 1 cut(s) 197
GluI GCNGC 6 cut(s) 111, 572, 575, 587, 686, 689
HaeIII GGCC 3 cut(s) 125, 640, 889
HhaI GCGC 1 cut(s) 198
Hin1II CATG 6 cut(s) 52, 102, 302, 713, 725, 847
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 132, 364, 434, 799
HphI GGTGA 3 cut(s) 150, 350, 617
Hpy166II GTNNAC 4 cut(s) 71, 152, 319, 562
Hpy188I TCNGA 2 cut(s) 258, 369
Hpy188III TCNNGA 5 cut(s) 9, 60, 710, 794, 844
Hpy8I GTNNAC 4 cut(s) 71, 152, 319, 562
HpyAV CCTTC 3 cut(s) 165, 479, 652
HpyCH4III ACNGT 2 cut(s) 77, 430
HpyCH4IV ACGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 3 cut(s) 583, 595, 886
HpyF3I CTNAG 4 cut(s) 21, 225, 257, 771
HpySE526I ACGT 1 cut(s) 64
Hsp92II CATG 6 cut(s) 52, 102, 302, 713, 725, 847
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 3 cut(s) 5, 51, 790
LmnI GCTCC 1 cut(s) 854
Lsp1109I GCAGC 5 cut(s) 558, 586, 598, 675, 697
MabI ACCWGGT 1 cut(s) 160
MaeII ACGT 1 cut(s) 64
MaeIII GTNAC 3 cut(s) 77, 502, 694
MalI GATC 3 cut(s) 7, 53, 792
MbiI CCGCTC 1 cut(s) 325
MboI GATC 3 cut(s) 5, 51, 790
MboII GAAGA 2 cut(s) 245, 373
MhlI GDGCHC 2 cut(s) 642, 843
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 9 cut(s) 32, 263, 291, 439, 451, 539, 617, 705, 743
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 1 cut(s) 141
MnlI CCTC 5 cut(s) 621, 764, 780, 828, 845
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 3 cut(s) 390, 864, 898
MslI CAYNNNNRTG 2 cut(s) 97, 603
Msp20I TGGCCA 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 688
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 583, 595, 886
NcoI CCATGG 1 cut(s) 98
NdeII GATC 3 cut(s) 5, 51, 790
NlaIII CATG 6 cut(s) 52, 102, 302, 713, 725, 847
NlaIV GGNNCC 4 cut(s) 27, 640, 752, 813
NmuCI GTSAC 1 cut(s) 502
OliI CACNNNNGTG 1 cut(s) 603
PagI TCATGA 2 cut(s) 709, 843
PceI AGGCCT 1 cut(s) 889
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 3 cut(s) 364, 434, 799
PflMI CCANNNNNTGG 1 cut(s) 652
PkrI GCNGC 6 cut(s) 112, 573, 576, 588, 687, 690
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 751
Psp5II RGGWCCY 1 cut(s) 751
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 4 cut(s) 27, 640, 752, 813
PspOMI GGGCCC 1 cut(s) 638
PspPI GGNCC 3 cut(s) 638, 639, 751
PspPPI RGGWCCY 1 cut(s) 751
PstI CTGCAG 2 cut(s) 484, 576
PstNI CAGNNNCTG 1 cut(s) 698
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 3 cut(s) 318, 563, 632
RsaNI GTAC 3 cut(s) 317, 562, 631
RseI CAYNNNNRTG 2 cut(s) 97, 603
SaqAI TTAA 3 cut(s) 390, 864, 898
SatI GCNGC 6 cut(s) 111, 572, 575, 587, 686, 689
Sau3AI GATC 3 cut(s) 5, 51, 790
Sau96I GGNCC 3 cut(s) 638, 639, 751
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 2 cut(s) 642, 843
SexAI ACCWGGT 1 cut(s) 160
SfcI CTRYAG 2 cut(s) 480, 572
SinI GGWCC 1 cut(s) 751
SmiMI CAYNNNNRTG 2 cut(s) 97, 603
Sse9I AATT 9 cut(s) 32, 263, 291, 439, 451, 539, 617, 705, 743
SseBI AGGCCT 1 cut(s) 889
SsiI CCGC 2 cut(s) 110, 325
StuI AGGCCT 1 cut(s) 889
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 2 cut(s) 77, 430
TaiI ACGT 1 cut(s) 67
TasI AATT 9 cut(s) 32, 263, 291, 439, 451, 539, 617, 705, 743
TatI WGTACW 2 cut(s) 316, 561
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 364, 434, 799
Tru1I TTAA 3 cut(s) 390, 864, 898
Tru9I TTAA 3 cut(s) 390, 864, 898
TscAI CASTG 4 cut(s) 80, 507, 535, 887
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 5 cut(s) 571, 574, 586, 685, 688
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 5 cut(s) 231, 327, 698, 710, 860
TspRI CASTG 4 cut(s) 80, 507, 535, 887
Van91I CCANNNNNTGG 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 751
XapI RAATTY 3 cut(s) 263, 539, 705
XcmI CCANNNNNNNNNTGG 2 cut(s) 62, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.